• 제목/요약/키워드: nanoprecipitation

검색결과 6건 처리시간 0.024초

All-trans Retinoic Acid Release from Surfactant-free Nanoparticles of Poly(DL-lactide-co-glycolide)

  • Jeong, Young-Il;Kim, Don-Gon;Jang, Mi-Kyeong;Nah, Jae-Woon;Kim, Yong-Bae
    • Macromolecular Research
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    • 제16권8호
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    • pp.717-724
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    • 2008
  • In this study, we prepared all-trans retinoic acid (ATRA)-encapsulated, surfactant-free, PLGA nanoparticles. The nanoparticles were formed by nanoprecipitation process, after which the solvent was removed by solvent evaporation or dialysis method. When a nanoparticle was prepared by the nanoprecipitation - solvent evaporation method, the nanoparticles were bigger than the nanoparticles of the nanoprecipitation - dialysis method, despite the higher although loading efficiency. Nanoparticles from the nanoprecipitation - dialysis method were smaller than 200 nm in diameter, while the loading efficiency was not significantly changed. Especially, nanoparticles prepared from DMAc, 1,4-dioxane, and DMF had a diameter of less than 100 nm. In the transmission electron microscopy (TEM) observations, all of the nanoparticles showed spherical shapes. The loading efficiency of ATRA was higher than 90% (w/w) at all formulations with exception of THF. The drug content was increased with increasing drug-feeding amount while the loading efficiency was decreased. In the drug release study, an initial burst was observed for $2{\sim}6$ days according to the variations of the formulation, after which the drug was continuously released over one month. Nanoparticles from the nanoprecipitation - dialysis method showed faster drug release than those from the nanoprecipitation - solvent evaporation method. The decreased drug release kinetics was observed at lower drug contents. In the tumor cell cytotoxicity test, ATRA-encapsulated, surfactant-free, PLGA nanoparticles exhibited similar cytotoxicity with that of ATRA itself.

Formation of Poly(ethylene glycol)-Poly($\varepsilon$-caprolactone) Nanoparticles via Nanoprecipitation

  • Lee, Jae-Sung;Hwang, Su-Jong;Lee, Doo-Sung;Kim, Sung-Chul;Kim, Duk-Joon
    • Macromolecular Research
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    • 제17권2호
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    • pp.72-78
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    • 2009
  • Size control of therapeutic carriers in drug delivery systems has become important due to its relevance to biodistribution in the human body and therapeutic efficacy. To understand the dependence of particle size on the formation condition during nanoprecipitation method, we prepared nanoparticles from biodegradable, amphiphilic block copolymers and investigated the particle size and structure of the resultant nanoparticles according to various process parameters. We synthesized monomethoxy poly(ethylene glycol)-poly($\varepsilon$-caprolactone) block copolymer, MPEG-PCL, with different MPEG/PCL ratios via ring opening polymerization initiated from the hydroxyl end group of MPEG. Using various formulations with systematic change of the block ratio of MPEG and PCL, solvent choice, and concentration of organic phase, MPEG-PCL nanoparticles were prepared through nanoprecipitation technique. The results indicated that (i) the nanoparticles have a dual structure with an MPEG shell and a PCL core, originating from self-assembly of MPEG-PCL copolymer in aqueous condition, and (ii) the size of nanoparticles is dependent upon two sequential processes: diffusion between the organic and aqueous phases and solidification of the polymer.

코아 가교 양친성 고분자 나노입자를 이용한 고함량 유용 약물 담지 고분자 나노입자 제조 (Preparation of Valuable Compounds Encapsulated Polymer Nanoparticles with High Payload Using Core-crosslinked Amphiphilic Polymer Nanoparticles)

  • 김나혜;김주영
    • 공업화학
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    • 제27권1호
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    • pp.26-34
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    • 2016
  • 본 연구에서는 반응성 비닐기를 가지고 있는 반응성 양친성 고분자 전구체(Reactive Amphiphilic Reactive Polymer Precursor) (RARP)를 이용하여 제조된 소수성 세그먼트들이 가교된 코아 가교 양친성 고분자(Core-crosslinked Amphiphilic Polymer) (CCAP) 나노입자와 나노침전법을 사용하여서 소수성 유용물질을 고함량으로 담지할 수 있는 새로운 공정을 제안하였다. 극성이 각기 다른 유기용매(에탄올, 아세톤, 테트라하이드로퓨란(THF))들과 소수성 세그먼트 분자량이 다른 CCAP를 사용하여서, 모델 유용 약물인 ${\alpha}$-tocopherol의 담지 효율, 담지량 및 약물 담지 나노입자의 크기와 안정성 변화를 조사하였다. 소수성 세그먼트 분자량이 큰 CCAP와 소수성 용매인 THF를 용매로 사용한 경우에 가장 높은 유용 약물 담지량, 담지 효율을 나타내는 안정한 나노입자가 형성이 되었다. 즉 CCAP 나노입자들의 물리적 화학적으로 견고한 나노 구조로 인해서 33 wt%의 높은 담지량과 97% 이상의 담지 효율을 가지면서 물속에서 70 nm의 크기의 안정한 유용 약물 담지 고분자 나노입자를 제조할 수 있었다.

수분산 공액고분자 나노입자의 합성 방법론 (Methods to Formulate Waterborne Conjugated Polymer Nanoparticles)

  • 강승주;강보석
    • 접착 및 계면
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    • 제24권1호
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    • pp.1-8
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    • 2023
  • 공액고분자는 유기전자소자부터 바이오메디칼 응용분야까지 다양하게 적용가능한 차세대 반도체소재이다. 하지만 낮은 수용성으로 주로 유기용매에 녹여 사용하나 최근 유기 용매의 독성과 환경 문제 이슈로 공액고분자의 수분산 나노입자화가 주목받고 있다. 본 총설에서는 나노입자가 형성되는 두 가지 원리와 이를 이용한 대표적인 공액고분자 나노입자 합성방법들 및 관련 연구들을 소개하고자 한다.

Docetaxel-loaded PLGA nanoparticles to increase pharmacological sensitivity in MDA-MB-231 and MCF-7 breast cancer cells

  • Tran, Phuong;Nguyen, Thu Nhan;Lee, Yeseul;Tran, Phan Nhan;Park, Jeong-Sook
    • The Korean Journal of Physiology and Pharmacology
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    • 제25권5호
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    • pp.479-488
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    • 2021
  • This study aimed to develop docetaxel (DTX) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (DTX-NPs) and to evaluate the different pharmacological sensitivity of NPs to MCF-7 and MDA-MB-231 breast cancer cells. NPs containing DTX or coumarin-6 were prepared by the nanoprecipitation method using PLGA as a polymer and d-α-tocopherol polyethylene glycol 1000 succinate (TPGS) as a surfactant. The physicochemical properties of NPs were characterized. In vitro anticancer effect and cellular uptake were evaluated in breast cancer cells. The particle size and zeta potential of the DTX-NPs were 160.5 ± 3.0 nm and -26.7 ± 0.46 mV, respectively. The encapsulation efficiency and drug loading were 81.3 ± 1.85% and 10.6 ± 0.24%, respectively. The in vitro release of DTX from the DTX-NPs was sustained at pH 7.4 containing 0.5% Tween 80. The viability of MDA-MB-231 and MCF-7 cells with DTX-NPs was 37.5 ± 0.5% and 30.3 ± 1.13%, respectively. The IC50 values of DTX-NPs were 3.92- and 6.75-fold lower than that of DTX for MDA-MB-231 cells and MCF-7 cells, respectively. The cellular uptake of coumarin-6-loaded PLGA-NPs in MCF-7 cells was significantly higher than that in MDA-MB-231 cells. The pharmacological sensitivity in breast cancer cells was higher on MCF-7 cells than on MDA-MB-231 cells. In conclusion, we successfully developed DTX-NPs that showed a great potential for the controlled release of DTX. DTX-NPs are an effective formulation for improving anticancer effect in breast cancer cells.

실란 기능화 양친성 고분자 전구체를 이용한 소수성 항균물질 담지 나노 입자 제조 및 항균 특성 (Preparation of Hydrophobic Antimicrobal Compounds Encapsulated Nanoparticles Using Alkoxysilane-functionalized Amphiphilic Polymer Precursor and Their Antimicrobial Properties)

  • 김나혜;김주영
    • 접착 및 계면
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    • 제18권1호
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    • pp.13-24
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    • 2017
  • 본 연구에서는 50 wt%의 매우 높은 담지량과 70%의 높은 고형분을 가지면서도 물속이나 다양한 제품에 나노 입자 형태로 분산이 가능한 새로운 형태의 소수성 항균 물질 담지 나노 입자를 제조하였다. 실란 기능화 양친성 고분자 전구체(Alkoxysilane-functionalized Amphiphilic Polymer Precursor; 이하 AAPP)와 다양한 실란 화합물을 이용하여 전형적인 Hydrolytic Sol-Gel 공정으로 제조된 수분산 유-무기 하이브리드 나노 입자들을 제조하고, 이를 이용하여서 나노 침전법을 사용하여서 소수성 항균물질을 고함량으로 담지할 수 있는 새로운 공정으로 소수성 항균물질인 Eugenol이 담지된 유-무기 하이브리드 형태의 나노입자 제조하였다. 나노 입자 제조시 제조 조건의 변화에 따른 나노 입자들의 크기, 담지량, 항균 활성 및 방출거동 등에 영향을 미치는 인자들을 조사하였다. 나노 입자의 종류에 관계없이 Minimal Inhibitory Concentration (MIC)는 50 mg/ml로 동일하였고, 모든 균주에서 99 %에 해당하는 우수한 항균력과 Pseudomonas aeruginosa (PSE)를 제외하고는 2주 이상의 항균 지속력을 나타내었다. 특히, Tetraethoxysilane (TEOS)를 첨가한 경우에는 견고한 무기물 도메인으로 인해 가장 높은 담지량 (50 wt.%)과 서방출 (Sustained release)을 나타내었고, Hexanediol (HD)을 첨가한 경우에는 HD 자체의 항균력과 용매로서의 역할도 하였기 때문에 가장 높은 항균력과 70%의 고형분을 나타내었다.