통합 검색 | Korea Science

Kim, Young-Soo;Choi, Bong-Soo;Min, Kyung-Rak
- Archives of Pharmacal Research
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- 제15권1호
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- pp.48-51
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- 1992
Achromobacter xylosoxidans KF701 and Pseudomonas putida (NAH7) were significantly different in degradative capability of aromatic compounds including benzoates, biphenyls, and naphthalene. However, both of the bacterial strains can grown on catechol as the sole carbon and energy source. Catechol 2, 3-dioxygenase gene for naphthalene oxidation or biphenyl oxidation was cloned into Escherichia coli HB 701. A E. coli HB 101 clone containing catechol 2, 3-dioxygenase gene from P. putida (NAH7) contains a recombinant plasmid with 3.60kb pBR322 and 6-kb insert DNA. Another E. coli HB101 clone containing catechol 2, 3-dioxygenase gene from A. xylosoxidans KF 701 has a recombinant plasmid with 4.4kb pBR322 and 10-kb insert DNA. Physical maps of the recombinant plasmids were constructed, and catechol 2, 3-dioxygenase gene in the recombinant plasmide was further localized and subcloned int M13. The cloned-catechol 2, 3-dioxygenase game products were identified as yellow bands on nondenaturaing polyacrylamide gel after electrophoresis followed by activity staining with catechol solution.
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김관주;김정태;최승권
- 소음진동
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- 제10권5호
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- pp.843-848
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- 2000
BEA(Boundary Element Analysis) based on Kirchhoff-Helmholtz integral equation is widely used in the prediction of sound radiation problems of vibrating structures. Accurate estimation of sound pressure distribution by BEA can be [possible if and only if dynamic behavior of the relating structure was described correctly. Another plausible method of sound radiation phenomena could be the NAH(Nearfield Acoustic Holography) method. NAH also based on the identical governing equation with BEA could be one of the best acoustic imaging schemes but it has disadvantages of the complexity of measurement and of the need of large amount of measuring points. In this paper, modal expansion method is presented for taking accurate dynamic data of the structures efficiently. This method makes use of vibration principle an arbitrary dynamic behavior of the structure is described by the summation of that structures mode shapes which can be calculated by FEA easily and accurately. Sound pressure field from a vibration flat plate is calculated using the combination of vibration signal on that flat plate from experiment, and of the natural mode shapes form FEA. When sound pressure field from vibration signal is calculated the importance of the phase information was emphasized.
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