• ALGAE
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• 제27권1호
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• pp.55-62
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• 2012

A novel lectin specific to N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine was isolated from Bryopsis plumosa and named as BPL-4. Sodium dodecyl sulfate polyacrylamide gel electrophorese (SDS-PAGE) and matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) mass spectrometry data showed that this lectin was a monomeric protein with molecular weight 12.9 kDa. The N-terminal amino acid sequences of the lectin were determined by Edman degradation and the full cDNA sequence encoding this lectin was obtained using the degenerate primers designed from the amino acid sequence. The size of the cDNA was 414 bp containing single open reading frame (ORF) encoding the lectin precursor. The homology analysis showed that this lectin might belong to H lectin group. BPL-4 showed high sequence similarity (60.6%) to BPL-3, which is a previously reported lectin from the same species. The comparative analysis on the lectin's primary structure showed two conserved domains including one possible active domain of H lectin group.

• Food Science and Biotechnology
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• 제18권2호
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• pp.519-525
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• 2009

A bacterial strain (Strain N-08) capable of extracellularly producing high level of non-reducing oligosaccharide (NR-OS) isolated from soil. The strain was identified phylogenetically by 16S rDNA sequence analysis and found to be very close to Arthrobacter crystallopoietes. The high production of NR-OS was observed in the basal culture medium containing maltose as a sole carbon source. The NR-OS in culture supernatant was purified by glucoamylase treatment and Dowex-1 (OH.) ion exchange chromatography and its structure was characterized. This oligosaccharide consisted of only glucose. Methylation analysis indicated that this fraction was composed mainly of non-reducing terminal glucopyranoside. Matrixassisted laser-induced/ionization time-of-flight (MALDI-TOF) and electrospray ionization-mass spectrometry (ESI-MS)/MS analyses suggested that this oligosaccharide comprised non-reducing disaccharide unit with 1,1-glucosidic linkage. When this disaccharide was analyzed by $^1H$-NMR and $^{13}C$-NMR, it gave the same signals with $\alpha$-D-glucopyranosyl-(1,1)-$\alpha$-Dglucopyranoside. These results indicated that the NR-OS produced by A. crystallopoietes N-08 was ${\alpha}1$,${\alpha}1$-trehalose. This is the first report of the trehalose which can be produced directly from maltose by A. crystallopoietes N-08.

• E2M - 전기 전자와 첨단 소재
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• 제10권4호
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• pp.342-348
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• 1997

In this paper, the behavior of electron swarm parameters and energy distribution function of the discharge under high E/N condition in e$^{-10}$ -CF$_{4}$ gas have been analysed over the E/N range from 1-300(Td) by the MCS and BEq methods using set of electron collision cross section determined by the authors. The swarm parameters and energy distribution function have been calculated for the pulsed Townsend, steady-state Townsend and Time of Flight methods. The results gained that the value of electron swarm parameters such as the electron drift velocity, the electron ionization and attachment coefficients and longitudinal diffusion coefficients in agreement with the experimental and theoretical data for a range of E/N. The electron energy distribution function has been explained and analysed in e$^{-10}$ -CF$_{4}$ at E/N : 5, 10, 100, 200, 300(Td) for a case of the equilibrium region in the mean electron energy and respective set of electron collision cross sections. The validity of the results has been confirmed by TOF and SST methods.

• Journal of Chest Surgery
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• 제35권6호
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• pp.430-438
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• 2002

저체중은 많은 심기형의 수술에 있어 위험인자로 알려져 있다. 이에 저체중 출생환아에 있어서의 동맥관 개존증을 제외한 여러 심기형에 대한 외과적 치료의 결과를 조사하였다. 대상 및 방법： 1994년 9월부터 2002년 2월까지 31명의 저체중 출생아를 대상으로 하였다. 심폐기를 이용한 개심술 환아(OHS군)가 12명, 비개심술환아(CHS군)가 19명이었다. 이들 환아에 대하여 술 후 중단기 성적을 알아보기 위해 환아의 병력지를 기초로 후향적 조사를 시행하였다. 태내주수는 36.9주(32.3∼42 주)였고 수술당시 평균 나이는 32.1일(0∼87 일), 출생시 체중은 1972g(1100∼2500g), 수술시 체중은 2105g(1450∼2500g)으로 OHS군과 CHS군에 차이가 없었다. 심기형은 OHS군에서는 심실중격결손증(VSD) (n=3), VSD와 대동맥궁이상(n=2), 총폐정맥환류이상(n=2), 대혈관전위증(n=2), 동맥간증(truncus arteriosus)(n=2), 삼심방증을 동반한 단심실증(n=1) 등이었고 CHS군은 대동맥축착증(n=7), 활로 4징증(n=3),활로 4징증 및 폐동맥폐쇄증(n=3), 다발성 근성 심실중격결손(n=1), 양대혈관 우심실기시증(n=1), 온전한 심실중격이 폐동맥판폐쇄증(n=2), 삼첨판폐쇄증(n=1), 대혈관전위증 및 다발성 심실중격결손(n=1) 등이었다. 13명(41.9%)의 환아에서 술 전 인공호흡이 필요하였다. 결과： 전체적으로 4례의 조기사망(30일 이내)이 발생하였다. OHS군에서 1례(8.3%), CHS군에서는 3례(15.8%)였다. 이 조기사망의 모든 예가 폐동맥교약술을 한 환아였고 완전교정이 가능하였던 환아나 폐동맥교약술을 제외한 고식적 수술에서는 수술사망이 없었다. 지연흉골봉합이 3례에서 필요하였고 술 후 7일 이상 장기간 인공호흡이 필요하였던 경우가 OHS군과 CHS 군에서 각각 7례로 58.3%, 38.8%의 빈도였다. 만기사망이 3례 발생하였는데 이 중 2례는 심장과 관련이 없는 사망이었다. OHS군 1명에서 술 후 뇌합병증이 발견되었다. 2명을 제외한 모든 생존자에서 현재 NYHA class I의 상태로 성장하고 있다. 결론： 저체중 출생 환아에서의 심기형은 완전교정술이 가능한 경우와 폐동맥교약술을 제외한 고식적 수술이 필요한 경우 낮은 수술사망률로 교정될 수 있었으며 중기성적도 양호하였다. 그러나 술 후 비교적 높은 빈도에서 장기간의 인공호흡이 필요하였다.

• 생명과학회지
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• 제27권1호
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• pp.50-56
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• 2017

졸복(Takifugu pardalis)의 아가미로부터 항균성 펩타이드를 정제하였다. 졸복 아가미의 산 추출물은 Sep-Pak C18에 의해 부분적으로 정제되었으며, 60% 메탄올 분획(RM60)은 Bacillus subtilis KCTC 1021에 대해 높은 항균활성을 나타내었다. 이 RM60을 사용하여 이온교환을 포함한 5단계의 연속적인 HPLC로 정제하였다. 정제된 펩타이드의 분자량과 아미노산 서열분석은 MALDI-TOF MS와 에드만 분해법으로 분석하였다. 이 펩타이드의 분자량은 약 1171.6 Da이었으며, 분석된 이 물질의 부분적인 일차구조서열은 다음과 같다; STKEKAPRKQ. 이 물질과 기존에 알려진 항균성 펩타이드들과 유사성을 조사한 결과, 정제된 물질은 histone H3 계열의 N-말단 부분과 유사하였다. 이러한 결과로부터 정제된 펩타이드는 졸복에서 반응하는 선천성 방어 시스템에서 중요한 역할을 하고 있다고 여겨진다.

• 한국방사선학회논문지
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• 제10권5호
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• pp.337-341
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• 2016

중성자에너지 영역 0.003 eV에서 10 eV에 대해 천연 Sm의 Sm(n,${\gamma}$) 반응에 대한 중성자 포획단면적을 측정하였다. 교토대학교 원자로실험소의 46-MeV 전자선형가속기에서 발생되는 전자의 광핵반응에 의한 중성자를 사용하였고 TOF 방법으로 측정하였다. 사용한 검출기는 12개의 BGO($Bi_4Ge_3O_{12}$) 섬광체로 구성되었고 이 검출장치로 Sm(n,${\gamma}$) 반응으로부터 나오는 즉발감마선을 측정하였다. 검출장치는 중성자 생성 위치로부터 $12.7{\pm}0.02m$ 위치에 설치되었으며 $^{10}B(n,{\alpha}{\gamma})^7Li$ 반응을 이용해 Sm 시료에 입사되는 중성자 선속을 구하였다. 또한 중성자 선속의 변화를 확인하기 위해 $BF_3$ 검출기로 모니터링 하였다. Sm(n,${\gamma}$) 반응단면적 측정결과는 BROND 2.2에 의한 평가결과와 J. C. Chou 및 V. N. Kononov 의 측정값과 비교하였다.

• Clinical and Experimental Pediatrics
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• 제61권11호
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• pp.362-365
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• 2018

Purpose: This study aimed to analyze changes in QRS duration and cardiothoracic ratio (CTR) following pulmonary valve replacement (PVR) in patients with tetralogy of Fallot (TOF). Methods: Children and adolescents who had previously undergone total repair for TOF (n=67; median age, 16 years) who required elective PVR for pulmonary regurgitation and/or right ventricular out tract obstruction were included in this study. The QRS duration and CTR were measured pre- and postoperatively and postoperative changes were evaluated. Results: Following PVR, the CTR significantly decreased (pre-PVR $57.2%{\pm}6.2%$, post-PVR $53.8%{\pm}5.5%$, P=0.002). The postoperative QRS duration showed a tendency to decrease (pre-PVR $162.7{\pm}26.4$ msec, post-PVR $156.4{\pm}24.4$ msec, P=0.124). QRS duration was greater than 180 msec in 6 patients prior to PVR. Of these, 5 patients showed a decrease in QRS duration following PVR; QRS duration was less than 180 msec in 2 patients, and QRS duration remained greater than 180 msec in 3 patients, including 2 patients with diffuse postoperative right ventricular outflow tract hypokinesis. Six patients had coexisting arrhythmias before PVR; 2 patients, atrial tachycardia; 3 patients, premature ventricular contraction; and 1 patient, premature atrial contraction. None of the patients presented with arrhythmia following PVR. Conclusion: The CTR and QRS duration reduced following PVR. However, QRS duration may not decrease below 180 msec after PVR, particularly in patients with right ventricular outflow tract hypokinesis. The CTR and ECG may provide additional clinical information on changes in right ventricular volume and/or pressure in these patients.

• Neonatal Medicine
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• 제18권2호
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• pp.365-369
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• 2011

폐동맥폐쇄가 동반된 팔로네증은 팔로네증 중에 가장 심한 형태이다. 이런 종류의 선천성 심질환에서 폐혈액 순환을 지속하기 위하여 동맥관 개존을 유지하는 것은 생명을 구하는 매우 중요한 치료이다. Milrinone은 선택적 인산이에스테르효소 3 억제제이며 강력한 혈관 확장제로 알려져 있다. 저자들은 폐동맥 폐쇄가 동반된 팔로네증 신생아에서 폐순환을 지속시키기 위하여 동맥관 개방을 유지하는 치료로 milrinone을 성공적으로 사용한 증례를 보고한다. Milrinone은 동맥관 개방효과뿐 아니라, 심근수축 촉진, 이완 촉진, 폐혈관 확장 효과가 있으며 부작용은 심하지 않아 더 이로운 치료방법이 될 가능성이 있다. Milrinone의 치료효과를 밝히기 위하여 더 많은 치료증례 조사와 타 약제와의 비교 연구 등이 필요하다.

• BMB Reports
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• 제39권1호
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• pp.46-54
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• 2006

The coenzyme A-acylating 2-oxoacid:ferredoxin oxidoreductase and ferredoxin (an effective electron acceptor) were purified from the hyperthermophilic archaeon, Sulfolobus solfataricus P1 (DSM1616). The purified ferredoxin is a monomeric protein with an apparent molecular mass of approximately 11 kDa by SDS-PAGE and of $11,180{\pm}50$ Da by MALDI-TOF mass spectrometry. Ferredoxin was identified to be a dicluster, [3Fe-4S][4Fe-4S], type ferredoxin by spectrophotometric and EPR studies, and appeared to be zinc-containing based on the shared homology of its N-terminal sequence with those of known zinc-containing ferredoxins. On the other hand, the purified 2-oxoacid: ferredoxin oxidoreductase was found to be a heterodimeric enzyme consisting of 69 kDa $\alpha$ and 34 kDa $\beta$ subunits by SDS-PAGE and MALDI-TOF mass spectrometry. The purified enzyme showed a specific activity of 52.6 units/mg for the reduction of cytochrome c with 2-oxoglutarate as substrate at $55^{\circ}C$, pH 7.0. Maximum activity was observed at $70^{\circ}C$ and the optimum pH for enzymatic activity was 7.0 -8.0. The enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutyrate, and 2-oxoglutarate. Among the 2-oxoacids tested (pyruvate, 2-oxobutyrate, and 2-oxoglutarate), 2-oxoglutarate was found to be the best substrate with $K_m$ and $k_{cat}$ values of $163\;{\mu}M$ and $452\;min^{-1}$, respectively. These results provide useful information for structural studies on these two proteins and for studies on the mechanism of electron transfer between the two.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1635-1642
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• 2013

Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (${\geq}$ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culture-positive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.