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http://dx.doi.org/10.5352/JLS.2017.27.1.50

Purification of an Antibacterial Peptide from the Gills of the Pufferfish Takifugu pardalis  

Kim, Tae Young (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University)
Go, Hye-Jin (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University)
Park, Nam Gyu (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University)
Publication Information
Journal of Life Science / v.27, no.1, 2017 , pp. 50-56 More about this Journal
Abstract
An antibacterial peptide was purified from an acidified gill extract of the pufferfish Takifugu pardalis. The acidified gill extract was put through a Sep-Pak C18 solid phase extraction cartridge using a stepwise gradient and divided into a flow-through (F.T.) and 60% methanol fraction (RM 60). Among the eluents, RM 60 had potent antibacterial activity against Bacillus subtilis KCTC 1021. RM 60 was partially purified on a cationic-exchange column (SP-5PW) by a linear gradient, and the antibacterial peptide was then further purified, using a series of cationic-exchange and $C_{18}$ reversed-phase HPLC columns. For characterization of the purified peptide, its molecular weight and amino acid sequence were analyzed by MALDI-TOF MS and Edman degradation. The molecular weight of the peptide was about 1171.6 Da. The amino acid sequence of the peptide was partially determined as: STKEKAPRKQ. A comparison of the N-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high homology with the N-terminus of the histone H3 protein, which belongs to the histone H3 family. Thus, this peptide was designated as a puffer fish gill (PFG)-related antimicrobial peptide. This is the report to describe an antimicrobial function for the N-terminus of histone H3 of an animal species. The findings suggest that this peptide plays a significant role in the innate defense system of the pufferfish.
Keywords
Antibacterial peptide; gill; pufferfish; purification;
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