• Ocean and Polar Research
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• 제26권2호
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• pp.273-286
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• 2004

The effects of environmental forcing on autotrophic picoplankton distributional patterns were investigated for convergence ($5^{\circ}N$), divergence ($9^{\circ}N-10^{\circ}30'N$) and oligotrophic ($17^{\circ}N$) sites in the tropical eastern Pacific during 2001 and 2003 KODOS (Korea Deep Ocean Study) cruises. The distributions of picoplankton populations - Prochlorococcus, Synechococcus and picoeukaryotes algae - were determined by flow cytometric analyses. Latitudinal variations in abundance maxima, vertical profiles, integrated abundance (0-150 m), and estimated carbon biomass were contrasted for each site according to three hydrological conditions. Prochlorococcus showed consistently high abundance in the surface mixed layers of all sites at $1\;{\times}\;10^5{\sim}3\;{\times}\;10^5\;cells\;ml^{-1}$ and showed declining abundance below these layers. However, these decreasing rates were not particularly sharp showing considerably high abundance at $1\;{\times}\;10^4\;cells\;ml^{-1}$ or higher even at 100 m depth. Vertical profiles of Synechococcus and picoeukaryotes were generally parallel to each other in all sites. A clear abundance maximum was observed at divergence site at or slightly above the pycnocline depth. Higher abundance was observed at the surface mixed layer for convergence site but a sharp decrease was observed below the pycnocline. However, there was no significant abundance fluctuation with depth at more oligotrophic site ($17^{\circ}N$). Integrated cell abundance of Prochlorococcus was high in the oligotrophic site at $2.17\;{\times}\;10^{13}\;m^{-2}$, and low in the convergence site at $0.88\;{\times}\;10^{13}\;m^{-2}$. However, opposite pattern was observed for Synechococcus and picoeukaryotes where relatively high integrated cell abundance was shown in the convergence site. Estimated carbon biomass of Prochlorococcus contributed 30.4-80.3% of total autotrophic picoplankton carbon showing the highest contribution in the oligotrophic site and the lowest contribution in the convergence site. Synechococcus contribution of total autotrophic picoplantkon carbon biomass was lower than 5.8% for most of sites except the convergence site where Synechococcus contributed 23.2% of picoplankton carbon biomass. Carbon biomass of picoeukaryotes was 18.8-46.4% showing the highest carbon biomass at the convergence site. Overall, Prochlorococcus showed higher cell abundance and carbon biomass and exhibited different reaction to hydrological conditions when compare with the other two major autotrophic picoplankton groups.

• 정보학연구
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• 제10권2호
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• pp.1-9
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• 2007

This study analyzed major cities of Chung-cheong area in Korea, -Daejeon($36^{\circ}11'{\sim}36^{\circ}30'\;N$, $127^{\circ}15'{\sim}127{\circ}34'\;E$), Chungju($36^{\circ}54'{\sim}37{\circ}02'\;N$, $127^{\circ}52'{\sim}128^{\circ}02'\;E$), and Cheongju($36^{\circ}34'{\sim}36^{\circ}44'\;N$, $127^{\circ}22'{\sim}127^{\circ}34'\;E$)- their characteristics as a Information Site. The geology map, a summit level map, and a drainage network map are created and analyzed the Site. The results are as follows : First, the geology of these cities are related with the shapes and the characteristics of the inner basins. The relief of inner basin determines by the characteristics of the bedrocks. Second, the major cities are close to rivers and the size of the granite & alluvium area influences the relief energy of the urban areas. Finally, the drainage networks explains the process involved in forming the basin and development of urban area.

• Mass Spectrometry Letters
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• 제12권3호
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• pp.66-75
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• 2021

Interleukin-4 (IL-4) and IL-13 are cytokines secreted by immune cells. Cytokines induce the proliferation of macrophages or promote the differentiation of secretory cells. The initiation and progression of allergic inflammatory diseases, such as asthma, are dependent on cytokines acting through related receptor complexes. IL-4 and IL-13 are N-glycoproteins. Glycan structures in glycoproteins play important roles in protein folding, protein stability, enzymatic function, inflammation, and cancer development. Therefore, the glycan structure of IL-4 and IL-13 needs to be elucidated in detail for the development of effective therapies. We report the first attempt to characterize the site-specific N-glycosylation of recombinant IL-4 and IL-13 via liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The tandem mass spectra of intact N-glycopeptides were identified using the Integrated GlycoProteome Analyzer (I-GPA) platform, which can automatically and rapidly analyze multiple N-glycopeptides, including their glycan composition and amino acid sequences. The recombinant IL-4 and IL-13 were identified with amino acid sequence coverages of 84% and 96%, respectively. For IL-4, 52 glycoforms on one N-glycosylation site were identified and quantified. In IL-13, 232 N-glycopeptides from three N-glycosylation sites were characterized, with the site Asn52 being the most extensively glycosylated (~80%). The complex glycans were the most abundant glycan on IL-4 and IL-13 (~96% and 91%, respectively), and the biantennary glycans were the most abundant in both recombinant IL-4 and IL-13 proteins.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.1104-1107
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• 2020

The N-terminal domain of the Pseudomonas sp. FB15 phytase increases low-temperature activity and catalytic efficiency. In this study, the 3D structure of the N-terminal domain was predicted and substitutions for the amino acid residues of the region assumed to be the active site were made. The activity of mutants, in which alanine (A) was substituted for the original residue, was investigated at various temperatures and pH values. Significant differences in enzymatic activity were observed only in mutant E263A, suggesting that the amino acid residue at position 263 of the N-terminal domain is important in enzyme activity.

• Geomechanics and Engineering
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• 제34권1호
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• pp.29-41
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• 2023

The 3D geospatial modeling of geotechnical information can aid in understanding the geotechnical characteristic values of the continuous subsurface at construction sites. In this study, a geostatistical optimization model for the three-dimensional (3D) mapping of subsurface stratification and the SPT-N value based on a trial-and-error rule was developed and applied to a dam emergency spillway site in South Korea. Geospatial database development for a geotechnical investigation, reconstitution of the target grid volume, and detection of outliers in the borehole dataset were implemented prior to the 3D modeling. For the site-specific subsurface stratification of the engineering geo-layer, we developed an integration method for the borehole and geophysical survey datasets based on the geostatistical optimization procedure of ordinary kriging and sequential Gaussian simulation (SGS) by comparing their cross-validation-based prediction residuals. We also developed an optimization technique based on SGS for estimating the 3D geometry of the SPT-N value. This method involves quantitatively testing the reliability of SGS and selecting the realizations with a high estimation accuracy. Boring tests were performed for validation, and the proposed method yielded more accurate prediction results and reproduced the spatial distribution of geotechnical information more effectively than the conventional geostatistical approach.

• Journal of Chest Surgery
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• 제26권5호
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• pp.343-348
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• 1993

Growth of suture line and anastomosis is required for long-term success after the tracheal and bronchial surgery in infant and pediatric patient. We used various suture materials in these cases, but the results were differ. To select the adequate suture material in tracheal surgery, we tried next. Tracheal anastomoses were performed in 150 Sprague Dawley rats, aged 4 to 8 [mean 5.8] weeks and weight 62 to 106[mean 83.6] gram, to compare polydioxanone[PDS] 7-0, polyglactin 910[Vicryl]7-0, and polypropylene [prolene] 8-0 suture materials. In 150 rats, only 29[20%] were lived over 300 days, and the weight was 250 to 320[mean 289.5]gram. Cross sectional area of the anastomoses and two or three tracheal rings below anastomosis site were measured under microscope, and calculated and compared as Hsieh`s equition. Cross-sectional area,anastomosis site/normal site 100, were 89.4 $\pm$ 5.34% in PDS group[n=9], 75.7 $\pm$ 6.06% in Vicrylgroup [n = 10], and 80.8$\pm$ 4.06% in Prolene group[n = 10]. Histopathologic studies were done for all autopsies or put in death around 300 days postoperatively. PDS absorblion was not seen 16 weeks after suture but disappeared over 24 weeks slide. Vicryl absorbtion was noted postoperative 8 to 16 weeks, with marked tissue reaction. Prolene showed least tissue reaction, but the suture material was persisted with regional fibrotic capsule.Causes of death were respiratory failure in 76 cases, tracheal rupture in 22 cases, hemorrhage, biting, starvation and etc. in 23 cases. With the brief review of literatures, we report the results.

• 생약학회지
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• 제32권3호통권126호
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• pp.212-218
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• 2001

The water extracts of 82 Korean medicinal herbs were prepared and were examined for the binding affinity on the glycine binding site of NMDA (N-methyl-D-aspartate) receptor prepared by the synaptic membranes from the forebrains of male Sprague-Dawley rats. Among the tested, the extracts of Dioscoreae Rhizoma, Hoveniae Semen cum Fructus, Astragali Radix, Armeniacae Semen, Huttuynia cordata Herba, Acanthopanacis Cortex, Aurantii nobilis Pericarpium, Phellinus linteus, Amomi Fructus, Artemisiae capillaris Herba, Polyporus, Agastachis Herba and of Galli Stomachichum Corium were found to exhibit significant competitions with $[^3H]-MDL$ 105,519 for the glycine specific binding site of NMDA receptor in a dose dependent manner, respectively.

• BMB Reports
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• 제40권1호
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• pp.44-52
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• 2007

Kinesins, as a kind of microtubule-based motor proteins, have a conserved microtubule-binding site in their motor domain. Here we report that two homologous kinesins in Arabidopsis thaliana, KatB and KatC, contain a second microtubule-binding site in their tail domains. The prokaryotic-expressed N-terminal tail domain of the KatC heavy chain can bind to microtubules in an ATP-insensitive manner. To identify the precise region responsible for the binding, a serious of truncated KatC cDNAs encoding KatC N-terminal regions in different lengths, KatC1-128, KatC1-86, KatC1-73 and KatC1-63, fused to Histidine-tags, were expressed in E. coli and affinity-purified. Microtubule cosedimentation assays show that the site at amino acid residues 74-86 in KatC is important for microtubule-binding. By similarity, we obtained three different lengths of KatB N-terminal regions, KatB1-384, KatB1-77, and KatB1-63, and analyzed their microtubule-binding ability. Cosedimentation assays indicate that the KatB tail domain can also bind to microtubules at the same site as and in a similar manner to KatC. Fluorescence microscopic observations show that the microtubule-binding site at the tail domain of KatB or KatC can induce microtubules bundling only when the stalk domain is present. Through pull-down assays, we show that KatB1-385 and KatC1-394 are able to interact specifically with themselves and with each other in vitro. These findings are significant for identifying a previously uncharacterized microtubule-binding site in the two kinesin proteins, KatB and KatC, and the functional relations between them.

• Journal of Korean Society for Atmospheric Environment
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• 제18권E4호
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• pp.191-201
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• 2002

The pH, electric conductivity. and the major ionic components were analyzed for the precipitation samples collected at 1100 Site of Mt. Halla and Jeju city. The quality of analytical data was verified by the comparison of ion balances, conductivities and acid fractions, all of which correlation coefficients were over 0.952. The ionic strengths lower than 10$^{-4}$ M were found in 57 and 28% at 1100 Site and Jeju city respectively. The precipitation in Jeju city was influenced more by the oceanic effect than those in 1100 Site. The acidification of precipitation was caused mostly by S $O_4$$^{2-}$and N $O_3$$^{[-10]}$ in both areas, and the organic acids have contributed to the acidity with only 7~8%. The neutralization factors by N $H_3$ were about 44 and 47％ at the 1100 site and the Jeju city, respectively, whereas those by CaC $O_3$were 21 and 24%, and the free acidity were about 38 and 28％ at two sites. From the investigation of seawater and soil enrichment factors, the S $O_4$$^{2-}$, N $O_3$$^{[-10]}$ and N $E_4$$^{+}$ were immigrated by other sources rather than from the seawater or soil origins. but not in the case of $Mg^{2+}$, C $l^{[-10]}$ , N $a^{+}$, and $K^{+}$. Factor analysis has shown that the precipitation at the 1100 site had been influenced mostly by anthropogenic sources, followed by soil and sea-water sources. On the other hand, the precipitation at the Jeju city was mainly influenced by oceanic sources, followed by anthropogenic and soil sources.urces.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.139-146
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• 2000

7- Bromomethylbenz[a]anthracene is a known mutagen and carcinogen. The two major DNA adducts produced by this carcinogen, i.e., $N^2$-(benz[a]anthracen-7-yl methyl)-2'-deoxyguanosine (2, b[a]$a^2$G) and $N^6$-(benz[a]anthracen-7-ylmethyl)-2'-deoxyadenosine (4, b[a]$a^6$/A), as wel 1 as the simpler benzylated analogs,$N^2$-benzyl-2'deoxyguanosine (1, $bn^2$G) and $N^6$-benzyl-2'-deoxyadenosine (3, $bn^6$/A), were prepared by direct aralkylation of 2'-deoxyguanosine and 2'-deoxyadenosine. To determine the site-specific mutagenicity of these bulky exocyclic amino-substituted adducts, the suitably protected nucleosides were incorporated into 16-base oligodeoxyribonucleotides in place of a normal guanine or adenine residues which respectively are part of the ATG initiation codon for the lac Z' \alpha-complementation gene by using an in situ activation approach and automated phosphite triester synthetic methods. The base composition and the incorporation of the bulky adducts into synthetic oligonucleotides were characterized after purification of the modified oligonucleotides by enzymatic digestion and HPLC analysis.