• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.127-130
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• 2004

We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.

• 생명과학회지
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• 제8권4호
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• pp.373-380
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• 1998

A considerable change observed in X-ray diffraction during the muscle contraction was that the movement of myosin head and conformational change of contractile monecules were occurred in the muscle contraction. Time slice requires tension peak after the onset of stimulation and the height of tension peak depends on the number of twitch cycle. The intensity of I$_{11}$, I$_{10}$, 143${\AA}$ reflection is measured with 5ms time resolution and is recorded in isometric tension. The peak height of I$_{11}$ and 143${\AA}$ intensity is changed after the onset of a stimulation I$_{i}$, and the length of twitch is shortened by successive twitches in the case of stimulation TI$_{i}$. On the other hand, the peak height of I$_{11}$ and 215${\AA}$ intensity starts to decrease at the 1st twitch and remains constant at low peak hight without appreciable recovery during the contraction term. In the case of uccessive twitch stimulation, the myosin heads of muscle are once moved from their resting position and never returned to their initial position.

• 한국임상수의학회지
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• 제32권1호
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• pp.62-68
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• 2015

There is no reliable indicator available for the diagnosis of horse laminitis, although the disease is common and costly. This study was performed to develop a specific diagnostic biomarker for laminitis. We have identified 33 differentially expressed proteins in plasma of a horse suffering laminitis that is experimentally induced by an overdose of oligofructose, in comparison with normal horse plasma. Among the proteins, myosin-9 mRNA was found in RNA sequencing analysis to be expressed specifically in laminitis tissues compared to other horse tissues. It is thus suggested that expression of plasma myosin-9 may be used for the diagnosis of equine laminitis.

• 한국응용과학기술학회지
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• 제24권4호
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• pp.362-368
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• 2007

To study the relationship between elementary biochemical states and structural states of the actomyosin crossbridges in muscle, the effects of binding of MgADP to myosin heads in the rigor muscle were examined by X-ray diffraction using synchrotron radiation. X-ray diffraction studies have been made to investigate the effects of binding of ADP on the structure of glycerinated rabbit skeletal muscle in the rigor state. The intensity increase was accompanied by a slight but distinct decrease in the 5.9 am layer-line intensity close to the meridian. These results strongly suggest that myosin heads altered their attached conformation in the proximal end toward the plane perpendicular to the fiber axis when MgADP was bound to them. We found that the intensity of the 14.5 nm-based meridional reflections increase by 20-50% when MgADP was added to the rigor muscle in the presence of hexokinase and myokinase inhibitor.

• PNF and Movement
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• 제6권1호
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• pp.41-51
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• 2008

Purpose : This paper reviews evidence supporting adaptive plasticity in skeletal muscle fibers induced by various exercise training and neuromuscular activity. Result : Skeletal muscle fiber demonstrates a remarkable adaptability and can adjust its physiologic and contractile makeup in response to alterations in functional demands. This adaptive plasticity results from the ability of muscle fibers to adjust their molecular, functional, and contractile properties in response to altered physiological demands, such as changes in exercise patterns and mechanical loading. The process of activity-dependent plasticity in skeletal muscle involves a multitude of signalling mechanisms initiating replication of specific genetic sequences, enabling subsequent translation of the genetic message and ultimately generating a series of myosin heavy chain isoform. Conclusions : Knowledge of the mechanisms and interaction of activity-dependent adaptive pathways in skeletal muscle is important for our understanding of the synthesis of muscle myosin protein, maintenance of metabolic and functional capacity with physical activity, and therapeutic intervention for functional improvement.

• 한국식품과학회지
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• 제21권2호
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• pp.173-179
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• 1989

본 연구는 도살직후 저장온도와 저장시간이 PSE이상 돈육의 단백질 변성에 미치는 영향을 DSC를 이용하여 육단백질의 열변성, 열에 대한 안정성과 근장단백질 용해성을 검토하였으며 그 결과를 요약하면 다음과 같다. 도살후 PSE육의 저장온도가 상승됨에 따라, 저장시간이 경과됨에 따라 DSC-thermodiagram의 myosin+sarcoplasmic protein의 변성이 나타나는 endotherm enthalpy가 감소하였다. 도살 후 $1{\sim}4$시간 동안 PSE육의 저장온도가 $20^{\circ}C$일 때 myosin+sarcoplasmic protein의 변성이 적었으나 $25^{\circ}C$ 이상일 때는 단백질 변성이 증가하였다. PSE육의 drip 손실은 도살 후 가능한 신속히 $2^{\circ}C$에 냉각시킨 육에서 최고를 나타냈다. 그러나 도살직후 수시간 동안 $32{\sim}38^{\circ}C$에 항온시킨 PSE육은 wateriness가 심하여 drip손실이 현저히 증가하였다. PSE육의 창백성은 도살직후 육온을 $20^{\circ}C$로 급속냉각함으로써 개선할 수 있었다. PSE육의 myosin+sarcoplasmic protein 의 변성이 심할 수록 발생하는 drip 양이 증가하였다.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 1997년도 제18차 정기총회 및 학술발표회
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• pp.94-94
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• 1997

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 1997년도 임시총회 및 제20차 추계학술발표회
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• pp.154-154
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• 1997

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 1997년도 임시총회 및 제20차 추계학술발표회
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• pp.155-155
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• 1997

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1703-1709
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• 2008

As genetic markers, single nucleotide polymorphisms (SNP) are very appropriate for the development of genetic tests for economic traits in livestock. Several microsatellite markers have been identified as useful markers for the genetic improvement of Hanwoo. Among those markers, ILSTS035 was recently mapped at a similar position with four SNPs (AH1_11, AH1_9, 31465_446, and 12273_165) in a linkage map of EST-based SNP in BAT6. Among the four SNPs, two SNPs (31465_446 and 12273_165) were analyzed using BLAST at the NCBI web site. The sequences including the 12273_165 SNP were identified at the intron region within the LOC534614 gene on the gene sequence map (Bos taurus NCBI Map view, build 3.1). The LOC534614 gene represents a protein similar to myosin heavy chain, fat skeletal muscle, embryonic isoform 1 in the dog, and myosin_1 (Myosin heavy chain D) in Macaca mulatta. In cattle, the myosin heavy chain was associated with muscle development. The phenotypic data for growth and carcass traits in the 415 animals were analyzed by the mixed ANCOVA (analysis of covariance) linear model using PROC GLM module in SAS v9.1. By the genotyping of Hanwoo individuals (n = 415) to evaluate the association of SNP with growth and carcass traits, it was shown that the 12273_165 SNP region within LOC534614 may be a candidate marker for growth. The results of the statistical analyses suggested that the genotype of the 12273_165 SNP significantly affected birth weight, weight of the cattle at 24 months of age, average daily gain and carcass cold weight (p<0.05). Consequently, the 12273_165 SNP polymorphisms at the LOC534614 gene may be associated with growth in Hanwoo, and functional validation of polymorphisms in LOC534614 should be performed in the future.