• Korean Journal of Microbiology
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• v.41 no.3
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• pp.164-167
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• 2005

The culture condition and medium composition for the enhanced mycelial growth of Lepista nuda DGUM 26501 were investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and $7.0\~8.0$, respectively. The partial pressure of oxygen for the enhanced mycelial growth was more than $10\%\;O_2$. When Czapek-Dox medium was used as a minimal medium, manitol and xylitol were very good carbon sources. Organic nitrogen sources were better than inorganic ones for mycelial growth. As the nitrogen source tested, com steep liquor, soytone and protease peptone were the best as a source of organic nitrogen sources. When ammonium phosphate as phosphorus sources was used, the enhanced mycelial growth was shown. Nicotinic acid was proved to be the most appropriate source of vitamin. After the mycelia of L. nuda DGUM 26501 was cultivated at $24^{\circ}C$ for 10 days in LNM broth (pH 7.0), the activities of extracellular enzyme were determined. The specific activity of $\alpha-amylase$ was much higher than those of other enzymes. However, little or no enzyme activities of $\beta-glucosidase$, CMCase, laccase and lipase were found.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.45-50
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• 2008

This study was carried out to investigate the promoting effect of wood flour on the mycelial growth of Lentinus lepideus and Lentinus edodes. To determine the optimal culture condition, we first examined the tissue origin of pine flour (Pinus densiflora) including needle, bark, root and xylem. Only the xylem-derived flour increased mycelial growth compared to no treatment control. The addition of the xylem flour (5 g/l) showed the highest increase and the glucose level in the basal medium was best at 10 g/l. The smaller particle size of the xylem flour showed the positive effect on mycelial growth; two-fold increase when supplemented with flour of which particle size is less than $106\;{\mu}m$ in diameter compared to $425\;{\mu}m$. The addition of the xylem flour continuously increased the mycelial production for 25 days while mycelia stopped growing within 15 days without the xylem flour. In addition, when woody flour obtained from the different tree species was applied to L. edodes mycelial culture, all treatments accelerated mycelial production compared to the control. Based on all results described above, we conclude that the supplementation of woody flour to culture medium may be an another promising way to increase mycelial production of economically important fungi.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.96.1-96
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• 2003

Serratia plymuthim A21-4 strongly inhibits the mycelial growth, zoospore formation, and cystospore germination of Phytophthor spp and Pythium species. The bacterial isolate produced antifungal substance and chitinase. The bacteria also enhanced to plant growth remarkably in low nutritional condition. The application of cell suspension of A21-4 to pepper seedlings in greenhouse experiments and soil drenching in farmer's field was proved successfully to control the phythophthora blight of pepper. For the effective control, however, relatively high density of cell number(10$\^$9/cfu/$m\ell$) is required. Density effect was similar in plant growth promoting activity of A21-4. Though this investigation we improved the problem with changes of culture condition of bacteria and some nutritional amendment.

• The Korean Journal of Mycology
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• v.35 no.2
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• pp.81-84
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• 2007

In some cases, the problem with the mycelium of Tricholoma giganteum is delayed mycelial growth or non-regeneration. Therefore, this study was conducted to understand the mycelial storage condition of T. giganteum and to investigate the regeneration ratio of mycelial growth. T. giganteum obtained from KACC in RDA was evaluated for its simple preservation at $10^{\circ}C$ and subcultured on different media. Mycelium of T. giganteum was cultured on the PBA (potato bamboo extract medium) for seven days at $30^{\circ}C$. Using simple preservation method, the mycelium of T. giganteum (MKACC 50852) and Pleurotus ostreatus (Chunchu No. 2) were stored on six different media in two kinds of storage vessels (tube and vial) for a period of 1-12 months at $4^{\circ}C,\;15^{\circ}C$, and $25^{\circ}C$ storage temperatures. At $4^{\circ}C$ storage condition, mycelial regeneration was failed in all agar media, but was successful in the sawdust medium for 3 months. At $15^{\circ}C$ storage, mycelial activity was maintained up to six months. On the other hand, P. ostreatus produced well-regenerated mycelia both at $4^{\circ}C$ and $15^{\circ}C$ storage up to 12 months. In conclusion, it is estimated that the mycelia storage condition of T. giganteum must be done at $15^{\circ}C$ and subcultured within six months.

• Journal of Mushroom
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• v.1 no.1
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• pp.21-27
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• 2003

The compost dextrose(CD) medium was selected a an optimum liquid culture media for Portobella(Agaricus brunescens). The optimal conditions for the mycelial growth of Agaricus brunescens were 25 and pH 7.0. Sucrose and soluble starch were favorable to mycelial growth. Malt extract and ammonium nitrate appeared to be more favorable than the other kinds of nitrogen source. The optimum C/N ratio as about 10.When the collected Portobella trains were compared with growth characteristics, KME 49007 appeared the highest of $44.6kg/3.3m^2$.

• Microbiology and Biotechnology Letters
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• v.19 no.2
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• pp.193-199
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• 1991

The effects of pH on the cell growth, the elaboration of pullulan, and the morphology of Aureobasidium pullulans IFO 4464 were examined. A. pulluans grew in yeast-like form at constant pH7.5 and in mycelial form at constant pH2.5. At the both pH conditions, the elaboration of pullulan was very low, about 6.0~6.5g/l. The mixture of yeast-like form and mycelial form of cells was found at the constant pH4.5, at which condition, the elaboration of pullulan was high, about 24.5g/l. The pH shift experimemts showed that the specific production rates of pullulan were 0.048($hr^{-1}$)for the mycelial form and 0.058($hr^{-1}$)for the yeast like form, which indicated that the yeast-like form has the similar, only slightly higher, biosynthetic activity of pullulan to the mycelial form at pH4.5 and the pH of culture broth is more important factor for the elaboration of pullulan than the morphology of A. pullulans.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.57-63
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• 2014

This study was carried out to obtain morphological and physiological characteristics of ear mushrooms for an artificial cultivation. Eighteen strains were cultivated with bag culture and classified into mainly five groups such as brown, black, white, purple and others group. The highest yield was shown in 43007 strain as 98.3 g/bag and 43009, 43016, 43025 and 44035 strains were more than 60 g/bag. Among collected strains, 43007, 43009 and 43035 were selected in this study as superior strains. Three selected strains were investigated for optimal mycelial growth conditions. MCM and GPYM media were selected for the favorable culture medium. The carbon sources of 43007, 43009 and 43035 on mycelial growth were maltose, fructose and glucose, respectively and peptone was selected as a nitrogen source. Highest mycelial growth was observed when the C/N ratio was 10 for 43007 and 20 for 43009 and 43035.

• The Korean Journal of Mycology
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• v.26 no.2 s.85
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• pp.187-193
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• 1998

The effects of medium, incubation temperature, incubation period, pH of medium and $CO_2$ ondition during mycelial growth were investigated to study the factors associated with the formation of oidia in Flammulina velutipes. Oidia formation was increased when mycelial growth was poor, while oidia formation was inhibited in optimum condition of mycelial growth. Mating type of oidia was investigated to examine the effect of oidia formation on dikaryotic strain. Di-mon matings between oidia strains and original dikaryotic strain were carried out. Monokaryotic strains derived from oidia showed only one genotype. Seventy percent among Dimon mating strains showed slow mycelial growth and low yield of fruit-body, but others showed similar or high mycelial growth and yields in comparision with original dikaryon strain. One strain from di-mon mating demonstrated some differences in isozyme band pattern.

• The Korean Journal of Mycology
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• v.39 no.2
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• pp.91-98
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• 2011

Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were $25^{\circ}C$ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.285-290
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• 2008

This study was carried out to obtain an optimal liquid culture condition for Sarcodon aspratus mycelia. Among various basal culture media the GYS (glucoe-yeast extract-soytone) medium was the best for mycelial growth. The appropriate temperature was $25^{\circ}C$. Starch, maltose or glucose was excellent carbon sources for the mycelial culture, compared to others tested. As nitrogen nutrients, soytone and $NH_4-N$ were the best organic and inorganic nitrogen sources, respectively. Moreover, the optimal concentration of soytone was 3 g per one-liter medium. In addition, we also found that alanine, $(NH_4)H_2PO_4$, and nicotinic acid were the best aminoacid, phosphorus salt, and vitamin, respectively. When all optimal conditions described above were applied to culture medium, we were able to produce 5.7 g dry weight of S. aspratus mycelia per one-liter liquid medium within 20 days.