DOI QR코드

DOI QR Code

목이류 인공재배를 위한 우량균주 선발 및 균사생장 최적 조건 구명

Selection of Superior Strains from Collected Ear Mushrooms for Artificial Cultivation and Their Optimal Condition of Mycelial Growth

  • 김정한 (경기도농업기술원 버섯연구소) ;
  • 이윤혜 (경기도농업기술원 버섯연구소) ;
  • 장명준 (경기도농업기술원 환경농업연구과) ;
  • 원선이 (경기도농업기술원 환경농업연구과) ;
  • 주영철 (경기도농업기술원 환경농업연구과)
  • Kim, Jeong-Han (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Lee, Yun-Hae (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Jang, Myoung-Jun (Division of Environmental Agricultural Research, Gyeonggido Agricultural Research & Extension Services) ;
  • Won, Seon-Yi (Division of Environmental Agricultural Research, Gyeonggido Agricultural Research & Extension Services) ;
  • Ju, Young-Cheoul (Division of Environmental Agricultural Research, Gyeonggido Agricultural Research & Extension Services)
  • 투고 : 2013.10.01
  • 심사 : 2014.01.27
  • 발행 : 2014.03.31

초록

목이의 국내 재배생산 기반을 마련하기 위해 국내외의 수집균주들의 재배적 특성을 조사하여 우량 균주를 선발하고, 선발된 계통에 대한 생리적 특성을 조사한 결과는 다음과 같다. 수집계통별 자실체 형태에서 갈색계통 9계통, 흑색계통 5계통, 백색계통 1계통, 털목이 1계통, 기타 2계통으로 크게 5그룹으로 분류되었다. 수량이 가장 우수한 계통은 43007균주로 98.3 g/bag이었고, 43009, 43016, 43025와 44035계통도 60 g/bag 이상이었다. 이 중에서 재배기간이 짧고 수량이 우수한 계통으로 흑색 계통 43009, 백색계통 43009, 털목이 계통 43035를 재배시험을 위한 우량균주로 선발하였다. 선발계통에 대한 균사생장을 위한 적합배지로는 43007균주는 MCM배지, 43009와 43035균주는 GPYM 배지로 나타났다. 배양온도는 43007와 43035균주가 $30^{\circ}C$, 43009균주가 $25{\sim}^{\circ}C$에서 균사생장이 우수하였다. 선발균주 별 탄소원은 43007균주는 maltose, glucsoe, fructose, 43009균주는 fructose, 43035균주는 glucose로, 균주별로 차이가 있었으며, 질소원은 선발계통 모두 peptone을 이용 시 균사생장이 우수하였다. 선발계통별 C/N율은 43007 균주는 10에서, 43009균주는 10~20에서, 43035균주는 20에서 균사생장이 빠른 것으로 나타났다.

This study was carried out to obtain morphological and physiological characteristics of ear mushrooms for an artificial cultivation. Eighteen strains were cultivated with bag culture and classified into mainly five groups such as brown, black, white, purple and others group. The highest yield was shown in 43007 strain as 98.3 g/bag and 43009, 43016, 43025 and 44035 strains were more than 60 g/bag. Among collected strains, 43007, 43009 and 43035 were selected in this study as superior strains. Three selected strains were investigated for optimal mycelial growth conditions. MCM and GPYM media were selected for the favorable culture medium. The carbon sources of 43007, 43009 and 43035 on mycelial growth were maltose, fructose and glucose, respectively and peptone was selected as a nitrogen source. Highest mycelial growth was observed when the C/N ratio was 10 for 43007 and 20 for 43009 and 43035.

키워드

참고문헌

  1. Sung JM, Yoo YB, Cha DY. Mushroom science. Seoul: Kyohak Publishing Co; 2000.
  2. Jo WS, Hwang EK, Kang MJ, Choi SY. Fruit-body production of Auricularia auricula-judae by sawdust cultivation. Kor J Mycol 2010;38:5-7. https://doi.org/10.4489/KJM.2010.38.1.005
  3. Lee JS, Ahn RM, Choi HS. Determinations of ergocalciferol and cholecalciferol in mushrooms. Kor J Soc Food Sci 1997; 13:173-178.
  4. KFDA. The list of inadequate food from collected 200 food in market; 2010.
  5. Yu YJ, Choi KH, Jeong JS, Lee GK, Choi SR. Study on characteristic of mycelial culture in ear mushroom. Kor J Mushroom Sci 2013;11:15-20. https://doi.org/10.14480/JM.2013.11.1.015
  6. Xu CP, Yun JW. Optimization of submerged-culture conditions for mycelial growth and exo-biopolymer production by Auricularia polytricha (wood ears fungus) using the methods of uniform design and regression analysis. Biotechnol Appl Boichem 2003;38:193-199. https://doi.org/10.1042/BA20030020
  7. Jonathan SG, Bowo DDS, Adehoye DO, Briyai OF. Studies on biomass production in Auricularia polytricha collected from Wilberforce Island, Bayelsa State, Nigeria. Am J Appl Sci 2009; 6:182-186. https://doi.org/10.3844/ajassp.2009.182.186
  8. Hong JS, Kwon YJ, Jung GT. Production of mushroom mycelium (Pleurotus ostreatus and Auricularia auricula-judae) in shaking culture. Kor J Mycol 1983;11:1-7.

피인용 문헌

  1. The culture conditions for the mycelial growth of Auricularia auricula-judae vol.12, pp.2, 2014, https://doi.org/10.14480/JM.2014.12.2.88