• Mycobiology
• /
• 제36권2호
• /
• pp.81-87
• /
• 2008

The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of $30^{\circ}C$ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, man nose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evaluate P. linteus mycelial colonization on logs, sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation techniques were used. Only sterilized short log inoculation produced good mycelial colonization. Initial mycelial growth and full mycelial colonization were best on 20 cm logs having 42% moisture content. The initial mycelial growth of P. linteus was accelerated over 12hr of sterilization. Basidiocarp formation was optimal using a burying method of logs after $5{\sim}6$ months, and fruiting body formation was superior in cultivation house conditions of $31{\sim}35^{\circ}C$ and in excess of 96% relative humidity.

• KSBB Journal
• /
• 제13권5호
• /
• pp.547-553
• /
• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• Journal of the Korean Wood Science and Technology
• /
• 제38권2호
• /
• pp.124-134
• /
• 2010

This study was performed to evaluate the efficiency of using softwood as the sawdust medium for Lentinula edodes cultivation, effect of nutrient on the mycelial growth, spawning, the mushroom yield, and quality. The nitrogen nutrition significantly enhanced the mycelial growth of L. edodes. The glutamic acid in the L. leptolepis and P. koraiensis, and asparagine in the P. densiflora were appeared to slight increase in the mycelial growth. The vegetable oil showed very effective on the mycelial growth in the P. koraiensis sawdust medium. Carbon/nitrogen ratio of all the test was reduced after mycelial growth. The mycelial growth was exclusively dependent on reduction of carbon. The mushroom yield (32.7%) of the P. densiflora sawdust medium (carbon source: 3% active carbon, nitrogen source: 0.4% asparagines) was the best in mushroom production of L. edodes, followed by the Q. variabilis sawdust (35.4%) of the control medium. The diameter of mushroom cap was obtained from the P. densiflora sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate) and P. koraiensis sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate), and the P. koraiensis sawdust (carbon source: 3% xylose, nitrogen source: 0.4% glutamic acid, supplement: 0.05% amino acid), with values 71.5 mm, 71.5 mm and 72.1 mm, respectively. In the polypropylene bag cultivation, the weight losses of the block medium gradually increased for 80 days in the dark (13.8~16.8%) and then became stable in the range of 20.7~25.8%.

• 산업기술연구
• /
• 제21권A호
• /
• pp.337-341
• /
• 2001

This study was carried out to screen the effective polymeric additives preventing wall growth during mycelial submerged cultivation of Ganoderma. lucidum. Effects of additives on mycelial growth and exo-polysaccharide (EPS) production in flask culture and jar fermenter system under 3 different pH processes were investigated, and changes of mycelial morphology were also examined. From flask culture of G. lucidum with additives of different concentrations, 0.1%(w/v) polyacrylic acid was effective for EPS production. As the polyacrylic acid of 0.1%(w/v) was added in medium, wall growth of G. lucidum mycelium grown in jar fermenter system could be protected. The addition of 0.1%(w/v) polyacrylic acid to medium was also improved the mycelial growth and EPS production in the later of submerged culture G. lucidum and no changes of mycelial morphology were observed.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2018년도 춘계학술대회 및 임시총회
• /
• pp.29-29
• /
• 2018

Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

• 한국균학회지
• /
• 제35권2호
• /
• pp.76-80
• /
• 2007

잎새버섯의 우량계통을 선발하여 농가에 보급하기 위하여 수집균주들의 균사체 생육 최적조건을 검토한 결과는 다음과 같다. 잎새버섯 수집균주의 생장에 적합한 배지는 PDA배지가 선발되었으며, 균사배양 적온은 $25^{\circ}C$로 나타났다. 잎새버섯의 균사생장에 적합한 수소이온 농도는 pH $4{\sim}5$로 약산성에서 균사생장이 우수하였다. 균사생장에 적합한 탄소원으로는 glucose와 fructose, 질소원으로는 peptone이 우수하였으며 C/N율은 $10{\sim}20$으로 질소 요구도가 다소 높은 것으로 나타났다.

• 한국버섯학회지
• /
• 제6권3_4호
• /
• pp.126-130
• /
• 2008

느타리버섯(수한, 춘추)의 음식부산물건조박 대체 효과실험을 위하여 기본배지(대조구)를 포플러톱밥 : 비트펄프 : 면실박을 5 : 3 : 2(v/v)로 하여 대조구의 면실박 20% 대신에 건조박을 10% 혼합하였을 때 10일간의 균사생장길이가 수한 1호는 63mm, 춘추 1호는 68mm이었다. 이는 대조구의 수한 1호 67mm, 춘추 1호 74mm 보다 균사생장속도는 약간 느리지만 균사밀도는 높아 대체하여 사용할 수 있음을 확인하였다. 그러나 건조박의 혼합비율이 20%와 30%로 높아질수록 균사생장속도는 늦어지고 균사밀도는 높아졌다.

• KSBB Journal
• /
• 제21권2호
• /
• pp.103-109
• /
• 2006

H. erinaceum의 기능성 소재화 연구의 일환으로, 지금까지 체계적으로 연구된 바 없는 H. erinaceum의 액체배양을 시도하고. 이 버섯의 서로 다른 생물반응기하의 액체배양 특성을 규명하고 대량생산을 검토하였다. 5 L 용량의 jar fermenter와 air-lift fermenter에서의 액체배양의 비교실험을 통해 air-lift fermenter의 사용이 종균 배양에 적합한 것으로 나타났으며, 형태 분석을 통해 적정 크기($2{\sim}4mm$)의 pellet 형태의 유지가 대량 생산의 중요인자인 것을 규명하였다. Air-lift fermenter 하의 배양을 종균으로 하고, 적정 크기의 pellet을 유지하는 500 L까지의 jar fermenter를 사용한 회분 배양의 scale-up 실험결과, 30 및 500 L에서의 교반 및 통기속도는 각각 200 rpm, 1 vvm 및 150 rpm, 1 vvm이었다. 이 조건하에서 배양의 전 기간 중 적정 pellet을 유지하였으며, 최대 균사체량은 약 $13{\sim}14g/L$이었다.

• 한국잠사곤충학회지
• /
• 제44권2호
• /
• pp.74-81
• /
• 2002

The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30$^{\circ}C$ and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35$^{\circ}C$ and over 96% of relative humidity.

• Journal of Microbiology and Biotechnology
• /
• 제9권6호
• /
• pp.726-731
• /
• 1999

It was discovered that ammonium phosphate in the medium played an important role in both growing mycelium and producing exopolysaccharides (EPS) from G. lucidum. In lower concentration levels of ammonium phosphate (0-3 g/l), an improved mycelial growth was observed by maintaining more filamentous morphology than in high concentrations (5-11 g/l). In addition, it was confirmed by comparing the factual dimension and frequency of the area regarding the mycelial pellets. This must be attributed to limitations of nutrient transfer by maintaining filamentous mycelium during the cultivation in a low ammonium phosphate containing medium. On the other hand, the best EPS production was observed in medium with the absence or low concentration of ammonium phosphate. The shear stress of the culture broth was greatly affected by the shear rate, as compared with that of the culture broth with high ammonium phosphate concentration. The rheological characteristics of the fermentation broth and filtrate worked well according to the Herschel-Bulkley model. It was also found that the morphological changes of the mycelium resulting from the ammonium phosphate concentration directly affected the rheological characteristics of the system and resulted in reversely affecting the EPS production levels. Based on these results, it can be concluded that delicate regulation of the ammonium phosphate concentration in the culture media should be provided in order to obtain optimal mycelial growth and/or EPS production.