• Mycobiology
• /
• v.37 no.2
• /
• pp.89-93
• /
• 2009

The effect of fermentation parameters and medium composition on the simultaneous mycelial growth and exo-polymer production from submerged cultures of Ganoderma applanatum was investigated in shake-flask cultures. The optimum initial pH for mycelial growth and exo-polymer production was 5.0 and 6.0, respectively. The optimum temperature was $25^{\circ}C$ and the optimum inoculum content was 3.0% (v/v). The optimal carbon and nitrogen sources were glucose and corn steep powder, respectively. After 12 days fermentation under these conditions, the highest mycelial growth was 18.0 g/l and the highest exo-polymer production was 3.9 g/l.

• Journal of the Korean Wood Science and Technology
• /
• v.31 no.6
• /
• pp.60-66
• /
• 2003

Mycelial growth of L. edodes by supplement nutrition of softwood was studied on a sawdust medium. The sawdust used was from the following softwood species : Larix leptolepis, Pinus densiflora and Pinus koraiensis. The added nutritions consisted of carbon nutritions(sucrose, active carbon, xylose, glucose, paper pellet), nitrogen nutritions(potassium nitrate, ammonium chloride, asparagine, glutamic acid) and vegetable oil(rice bran oil). The sawdust medium was a mixture of 76% sawdust, 20% rice bran, 3% carbon nutrition, 0.4% nitrogen nutrition and 0.6% calcium carbonate. Following addition of carbon and nitrogen nutritions on the sawdust medium proved most suitable : L. leptolepis (glucose, glutamic acid), P. densiflora (active carbon, asparagine) and P. koraiensis (xylose, glutamic acid). The highest mycelial growth was obtained from sawdust medium of optimum condition with 97% of L. leptolepis, 110% of P. densiflora and 98% of P. koraiensis. This study has provided useful preliminary information for the cultivation of L. edodes.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.12 no.1
• /
• pp.21-27
• /
• 2010

The objective of this study was to determine the effects of "Green-one" organic nutrient on mycelial growth and fruiting of P. ostreatus. The dilution concentrations of "Green-one" was treated as follows. There was control, 100, 200, 400 concentrations. That treatments were treated with step of each mycelial growth step. The best of growth steps was mycelial scratching step. At that time, DPI(Day required for primordial formation after inoculation) was shortened for 1 day. Valid germination stipe are 15 pieces, 3 pieces more than control. Stipe length and stipe diameter was long each 4mm, 3mm more than control. Pileus size is shortened than control. Yields per one bottle(g/850cc) was 146g increased 6.5% than control 137g/850cc.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.7 no.1
• /
• pp.74-80
• /
• 2005

To elucidate the optimal conditions of mycelial growth of Pholiota adiposa, the effect of a kinds of media, incubation temperature and shaking culture were investigated. Mushroom complete medium was selected as superior media for mycelial growth in this fungus. The optimal temperature for mycelial growth of this fungus shows at 25℃. Shaking culture could be obtain the mycelia in two or three times than the stationary culture.

• Journal of Mushroom
• /
• v.4 no.3
• /
• pp.122-127
• /
• 2006

This study was carried out to investigate the mycelial growth and density of A. auricula treated four kinds of substances L-citrulline, L-carnitine, Senna, Garcinia cambogia made from 10, 100, 500, 1000, 1500, 2000 times solutions respectively. Senna all treatments made from 10, 100, 500, 1000, 1500, 2000 times solutions are available for mycelial growth and density than control. Senna treatment made from 100 times solution of them is the best. L-citrulline treatment made from 500 times solution grew more much 7.86mm for 15 days than control, also it was optimal. L-carnitine treatment made from 100 times solution grew more much 3.4mm for 15 days than control, also it was optimal. Garcinia cambogia all treatments made from 10, 100, 500, 1000, 1500, 2000 times solutions were not available for mycelial growth and density than control. But Garcinia cambogia treatment made from 1000 times solution of them was similar to control. Accordingly, these solutions like that were effective to mycelial growth and density than control.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.58-58
• /
• 2002

This study was excuted to decide the physiological characteristics of Pleurotus Ferulae Lanzi. P. Ferulae Lanzi. was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of P. Ferulae Lanzi. was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 25~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 5.0~6.0. The required carbon and nitrogen source for mycelial growth of P. Ferulae Lanzi. was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was starch or maltose. And the nitrogen source for pertinent mycelial growth was yeast extract.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.59-59
• /
• 2002

This study was excuted to decide the physiological characteristics of Lentinus lepides. L. lepides was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of Lentinus lepides was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 22~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 3.0~4.0. The required carbon and nitrogen source for mycelial growth of Lentinus lepides was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was maltose. And the nitrogen source for pertinent mycelial growth was peptone.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.60-60
• /
• 2002

This study was excuted to decide the physiological characteristics of Fomers fomentarius. F. fomentarius was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of F. fomentarius was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 22~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 5.0~6.0. The required carbon and nitrogen source for mycelial growth of F. fomentarius was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was glucose or starch. And the nitrogen source for pertinent mycelial growth was yeast

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.61-61
• /
• 2002

This study was excuted to decide the physiological characteristics of Kuehneromyces mutabilis. K. mutabilis was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of K. mutabilis was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 25~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 5.0~6.0. The required carbon and nitrogen source for mycelial growth of K. mutabilis was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was mannose or glucose. And the nitrogen source for pertinent mycelial growth was yeast extract or peptone.

• The Korean Journal of Mycology
• /
• v.24 no.4 s.79
• /
• pp.322-328
• /
• 1996

It is possible to cultivate Pleurotus ostreatus on the crush medium of Reynoutria sachalinensis. The crush of leaves and stems of Reynoutria sachalinensis with water (1:8 W/V) enhanced mycelial growth of P. ostreatus. That mycelial growth of P. ostreatus on the crush medium was accelerated three times as fast as that on malt extract agar (MEA), and mycelial compactness was denser than that of on MEA. The same result was obtained on mixture of saw dust and the crush of leaves and stems in test tube and bottle. The addition of rice bran and the crush to saw dust was best for mycelial growth. Regardless of pH (4.5, 6.5 and 8.5), P. ostreatus could suppress the growth.