• Title/Summary/Keyword: mutant virus

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Isolation and Characterization of Defense Genes Mediated by a Pathogen-Responsive MAPK Cascade in Tobacco (담배에서 병원균에 반응하는 MAPK 신호전달체계에 의해 매개되는 방어 유전자들의 분리 및 특성화)

  • Jang, Eun-Kyoung;Kang, Eun-Young;Kim, Young-Cheol;Cho, Baik-Ho;Yang, Kwang-Yeol
    • Journal of Life Science
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    • v.18 no.8
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    • pp.1023-1030
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    • 2008
  • NtMEK2, which is the tobacco MAPK kinase that is upstream of SIPK and WIPK, was identified using the dexamethasone (DEX)-inducible gain-of-function transgenic system. Expression of $NtNEK2^{DD}$, a constitutively active mutant of NtNEK2, leads to HR-like cell death, which indicates that the NtMEK2-SIPK/WIPK cascade controls defense responses in tobacco. However, little is known about the downstream target substrates or defense-related genes that are regulated by the NtMEK2-SIPK/ WIPK cascade. In this study, ACP-based differential display RT-PCR was used to isolate the downstream effectors mediated by the NtMEK2-SIPK/WIPK cascade in $NtNEK2^{DD}$ transgenic plants. The results identified 6 novel differentially expressed genes (DEGs). These included pathogen induced protein 2-4 (pI2-4), monoterpene synthase 2 (MTS2), seven in absentia protein (SINA), cell death marker protein 1 (CDM1), hydroxyproline-rich glycoprotein (HRGP) and unknown genes (DEG45). The induction of these genes was confirmed by RT-PCR of samples obtained from $NtNEK2^{DD}$ plants. Additionally, when compared with other isolated DEGs, the pI2-4, CDM1 and HRGP genes were significantly up-regulated in response to treatment with salicylic acid and tobacco mosaic virus. Taken together, these results suggest that three novel DEGs were regulated by the NtMEK2-SIPK/WIPK cascade involved in disease resistance in tobacco.

Development of dry milling suitable rice cultivar to invigorate rice processing products

  • Jeung, Ji-Ung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.10-10
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    • 2017
  • Rice consumption has been continuously decreasing as the eating habits of Koreans have become westernized and diversified. The per capita annual rice consumption in Korea has dropped sharply from 136.4 kg in 1970 to 61.9 kg in 2016. The Korean government, therefore, has been trying to promote rice consumption by invigorating the processed food industry using rice flour. To facilitate the market for processed rice foods, it is essential to develop proper milling technology in terms of flour particle size and damaged starch content to produce high quality rice flour at competitive cost. Dry milling and wet milling are the two major processes used to produce rice flour. Although the dry milling process is relatively simple with a lower production cost, damaged starch content increases because of the high grain hardness of rice. In wet milling, the quality of rice flour is improved by reducing flour particle size as well as damaged starch content through soaking procedures. However, the production costs are high because of the additional expenses associated with the disposal of waste water, sterilization and drying of the wet flour. Recently developed technologies such as jet milling and cryogenic milling also require expensive investment and production. Therefore, developing new rice cultivars with dry milling adaptability as well as good processing properties is an important goal of rice breeding in Korea. 'Suweon 542' is a floury endosperm mutant line derived from sodium azide treatment on a high-yield, early maturing, and non-glutinous japonica rice cultivar, 'Namil'. Compared with the wild type, after dry milling process, the grain hardness of 'Suweon 542' was significantly lower because of its round and loosely packed starch granules. Also, the flour of 'Suweon 542' had significantly smaller particles and less damaged starch than 'Namil' and other rice cultivars and its particle size distribution was similar to a commercial wheat cultivar. Recently, through collaborations with nine universities and food companies, a total of 21 kinds of processed prototypes, using the dry milling flour of 'Suweon 542', were evaluated. In the production of major rice processing products, there was no significant quality difference between the flours prepared by wet milling and dry milling. Although the amount of water added to the dough was slightly increased, it was confirmed that the recipe applying the wet flour could be used without significant change. To efficiently transfer the floury endosperm characteristics of 'Suweon 542' to other commercial rice cultivars, it is essential to develop DNA marker tightly linked to the target gene. Association analysis using 70 genome-wide SSR markers and 94 F2 plants derived from 'Suweon 542'/'Milyang 23' showed that markers on chromosome 5 explained a large portion of the variation in floury grains percentage (FGP). Further analysis with an increased number of SSR markers revealed that the floury endosperm of 'Suweon 542' was directed by a major recessive locus, flo7(t), located in the 19.33-19.86 Mbp region of chromosome 5, with RM18639 explaining 92.2% of FGP variation in the F2 population. Through further physical mapping, a co-segregate and co-dominant DNA marker with the locus, flo7(t) was successfully developed, by which, thereby, breeding efficiency of rice cultivars having proper dry milling adaptability with high yield potential or useful functional materials would be improved. 'Suweon 542' maintained the early maturity of the wild type, Namil, which can be used in rice-wheat double cropping systems in Korea not only for improved arable land but also for sharing flour production facilities. In addition to the high susceptibility against major rice diseases, nevertheless, another possible drawback of 'Suweon 542' is the high rate of viviparous under prolonged rainfall during the harvesting season. To overcome susceptibility and vivipary of 'Suweon 542', the progeny lines, derived from the crosses 'Suweon 542' and 'Jopyeong', an early maturing rice cultivar with multiple resistance against rice blast, bacterial blight, and rice strip virus, and 'Heugjinju', a anthocyanin pigment containing black rice cultivar, were intensively evaluated. As the outputs, three dry milling suitable rice elite lines, 'Jeonju614', 'Jeonju615', and 'Jeonju616' were developed.

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A New Auricleless Barley Cultivar "Dami" for Whole Crop Forage (밀식적응 양질 다수성 무엽이 청보리 품종 "다미")

  • Park, Tae-Il;Seo, Jae-Hwan;Han, Ouk-Kyu;Park, Ki-Hun;Choi, Jae-Seong;Kim, Jung-Gon;Park, Jong-Chul;Kim, Hong-Sik;Heo, Hwa-Young;Baek, Seong-Bum;Kwon, Young-Up;Park, Hyoung-Ho;Kang, Moon-Seok;Park, Kwang-Geun;Suh, Sae-Jung
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.349-353
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    • 2009
  • We developed a new barley cultivar "Dami" (Hordeum vulgare L.) with the auricleless gene lig (al, li, aur-a). The characteristic of auricleless is a spontaneous mutant type which has known as a monogenic recessive gene. The plant with the gene has erect leaf blades because of no auricle. The cultivar was derived from a cross between 'BGS60' and 'Kangbori'. 'BGS60' has the auricleless gene (li), while 'Kangbori' showed a high biomass with winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus). Subsequent generations were handled by the bulk method in a pedigree selection program. A promising line showed both high yield and lodging resistance in the yield trials at Iksan in 2003 to 2004, and designated as Iksan414. The line was subsequently evaluated for winter hardiness, earliness, and yield in the seven locations around Korea for three years from 2005 to 2007 and was designated as "Dami" and released. It has the growth habit of III, erect plant type, green leaf and stem similar to the check cultivar 'Sunwoo' Its heading date was April 30, and maturing date May 31 in paddy field conditions, which were similar to those of 'Sunwoo' respectively. The cultivar Dami was 97 cm in culm length, had 643 spikes per $m^2$ and higher leaf dry weight, and better adaptability to dense planting, winter hardiness, and resistance to BaYMV than the check cultivar did. The average forage yield of "Dami" was about 12 ton $ha^{-1}$ in dry matter (33 ton $ha^{-1}$ in fresh matter) in paddy field. "Dami" also showed 7.5% of crude protein content, 28.5% of ADF (Acid Detergent Fiber), 50.1% of NDF (Neutral Detergent Fiber), and 66.4% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley. This cultivar would be suitable for the area where the daily minimum temperature of January is above $-8^{\circ}C$ in Korean peninsula.

A Lodging Tolerant, Opaque Rice Cultivar 'Seolgaeng' (벼 내도복 뽀얀멥쌀 신품종 '설갱')

  • Hong, Ha-Cheol;Moon, Huhn-Pal;Choi, Hae-Chune;Hwang, Hung-Goo;Kim, Yeon-Gyu;Kim, Hong-Yeol;Yea, Jong-Doo;Shin, Young-Seop;Kang, Kyung-Ho;Choi, Yong-Hwan;Cho, Young-Chan;Baek, Man-Kee;Yang, Chang-Ihn;Choi, Im-Soo;Ahn, Sang-Nag;Yang, Sae-June
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.532-537
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    • 2011
  • 'Seolgaeng' is a new Japonica rice variety developed from an 'Ilpumbyeo' mutant line, N-methyl-N-nitrosourea (MNU) treatment on fertilized egg cell, by a rice breeding team of National Crop Experiment Station, Rural Development Administration in 2001. This variety has about 134 days of growth duration from transplanting to harvesting in central plain area of Korea, a good semi-elect plant type and resistant to lodging with strong culm. The number of panicles/hill of 'Seolgaeng' is more than that of 'Hwaseongbyeo'. The milled rice appearance of this cultivar is an opaque, but it has amylose content (19.3%) similar to 'Hwaseongbyeo'. It is superior to hypae formation of Aspergillus orzyae in the making of fermented rice and amount of pigmentation in fermented rice by Monascus anka that of 'Ilpumbyeo'. This variety showed slow leaf senescence and considerable resistance to viviparous germination. It is moderately resistant to leaf blast and susceptible to bacterial blight, virus disease and insect pests. The yield performance of this rice cultivar is about 5.27 MT/ha in milled rice in local adaptability test for three years from 1999 to 2001). 'Seolgaeng' is adaptable to central and southern plain areas of Korea.

A New Mid-late Maturing Rice Variety, 'Honong' with High-Quality and Multiple Disease Resistance (중만생 고품질 복합내병성 벼 신품종 '호농')

  • Kim, Ki-Young;Shin, Mun-Sik;Ko, Jae-Kwon;Nam, Jeong-Kwon;Ha, Ki-Yong;Baek, Man-Gee;Ko, Jong-Cheol;Kim, Bo-Kyeong;Park, Hyun-Su;Shin, Woon-Chul;Kang, Hyun-Jung;Choung, Jin-Il;Kim, Woo-Jae;Mo, Young-Jun;Kim, Jung-Gon
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.252-256
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    • 2010
  • 'Honong' is a new japonica rice variety developed and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2009. This variety was derived from mutagen MNU (N-methyl-N-nitrosourea) treatment on fertilized egg cells of Unbong31. This variety has about 126 days growth duration from transplanting to harvesting in west-southern coast, Honam and Youngnam plain of Korea. It has 79 cm in culm length and is tolerant to lodging. In reaction to biotic and abiotic stresses, it show resistance to blast, bacterial blight pathogen races from $K_1$ to $K_3$ and stripe virus, but susceptible to other major diseases and insect pests. The milled rice of 'Honong' exhibits translucent, relatively clear non-glutinous endosperm and medium short grain. It has higher amylose content (20.3%) and lower protein content (6.3%), and good palatability of cooked rice compared with Nampyeong. The milled rice yield performance of this variety is about 5.44MT/ha in local adaptability test for three years. 'Honong' would be adaptable to west-southern coast, Honam and Youngnam plain of Korea.