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http://dx.doi.org/10.5352/JLS.2008.18.8.1023

Isolation and Characterization of Defense Genes Mediated by a Pathogen-Responsive MAPK Cascade in Tobacco  

Jang, Eun-Kyoung (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Kang, Eun-Young (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Kim, Young-Cheol (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Cho, Baik-Ho (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Yang, Kwang-Yeol (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Publication Information
Journal of Life Science / v.18, no.8, 2008 , pp. 1023-1030 More about this Journal
Abstract
NtMEK2, which is the tobacco MAPK kinase that is upstream of SIPK and WIPK, was identified using the dexamethasone (DEX)-inducible gain-of-function transgenic system. Expression of $NtNEK2^{DD}$, a constitutively active mutant of NtNEK2, leads to HR-like cell death, which indicates that the NtMEK2-SIPK/WIPK cascade controls defense responses in tobacco. However, little is known about the downstream target substrates or defense-related genes that are regulated by the NtMEK2-SIPK/ WIPK cascade. In this study, ACP-based differential display RT-PCR was used to isolate the downstream effectors mediated by the NtMEK2-SIPK/WIPK cascade in $NtNEK2^{DD}$ transgenic plants. The results identified 6 novel differentially expressed genes (DEGs). These included pathogen induced protein 2-4 (pI2-4), monoterpene synthase 2 (MTS2), seven in absentia protein (SINA), cell death marker protein 1 (CDM1), hydroxyproline-rich glycoprotein (HRGP) and unknown genes (DEG45). The induction of these genes was confirmed by RT-PCR of samples obtained from $NtNEK2^{DD}$ plants. Additionally, when compared with other isolated DEGs, the pI2-4, CDM1 and HRGP genes were significantly up-regulated in response to treatment with salicylic acid and tobacco mosaic virus. Taken together, these results suggest that three novel DEGs were regulated by the NtMEK2-SIPK/WIPK cascade involved in disease resistance in tobacco.
Keywords
Mitogen-activated protein kinase; NtMEK2- SIPK/WIPK cascade; plant defense signaling; defense-related gene; annealing control primer-based differential display reverse transcription polymerase chain reaction;
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