• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.721-725
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• 2001

Physiological activity in different parts of Dolsan leaf mustard (DLM) was investigated. To determine the physiological activity, DLM was crushed filtered centrifuged and then the supernatant was used as a sample. of 1%, 3% and 6%. By adding of improved root juice(20th day) with the concentration of 6% the cytotoxicity against HepG2 was the highest about 78.2%. And antioxidative effects in different parts of DLM was measured by using the DPPH method. Antioxidative effects was higher in all leaves than other parts. In particular antioxidative effects was the highest in leaves of traditional DLM at the 20th day of growth about 80.4%. In leaves of improved DLM at the 60th growth angiotensin-I converting enzyme inhibitory effect was the highest about 94.0%. Consequently there was not significant difference of physiological activity between improved and traditional DLM. However the cytotoxicity against HepG2 was the highest in roots of DLM. And the antioxidative and the ACE inhibitory effect in leaves of DLM were higher than those of other parts.

• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.137-143
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• 2009

An efficient system for high frequency plant regeneration was established through investigating various factors such as plant growth regulator combinations, explant types and ages, and addition of $AgNO_3$ influenced on shoot regeneration in Brassica juncea L. cv. BARI sarisha-10. Murashige and Skoog (MS) medium supplemented with 0.1 mg/L NAA (1-naphthaleneacetic acid) and 1 mg/L BA (6-benzyladenine) showed the maximum shoot regeneration frequency (56.67%) among the different combinations of NAA and BA. Explant type, explant age, and addition of $AgNO_3$ also significantly affected shoot regeneration. Of the four type of explants (cotyledon, hypocotyl, root, and leaf explants)- cotyledon explants produced the highest shoot regeneration frequency and hypocotyls explants produced the highest number of shoots per explant, whereas root explants did not produce any shoot. The cotyledonary explants from Four-day-old seedlings showed the maximum shoot regeneration frequency and number of shoots per explant. Shoot regeneration frequency increased significantly by adding $AgNO_3$ to the medium. Two mg/L $AgNO_3$ appeared to be the best for shoot regeneration with the highest shoot regeneration frequency (86.67%) and number of shoots per explant (7.5 shoots). Considerable variation in shoot regeneration from cotyledonay explants was observed within the B. juncea L. genotypes. The shoot regeneration frequency ranged from 47.78% for cv. Shambol to 91.11% for cv. Rai-5. In terms of the number of shoots produced per explant, B. juncea L. cv. Daulot showed the maximum efficiency. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency of rooting. The regenerated plantlets were transferred to pot soil and grown to maturity in the greenhouse. All plants were fertile and morphologically identical with the source plants.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.04a
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• pp.182-186
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• 2001

An acid forest surface soil as a land farming medium was treated with a water plant alum sludge at 0 to 18%. Indian mustard was grown in the treated soil in a greenhouse for 5 weeks and watered with pH 4 tap water adjusted with a mixed acid (1HNO$_3$: 2H$_2$SO$_4$) during plant growth. Changes in soil property, leachate chemistry, plant growth, and plant uptake of elements by the sludge treatment were determined. The alum sludge treatment increased buffer capacity to acidity, hydraulic conductivity, water holding capacity, and phosphate adsorption of the soil and decreased bulk density and mobility of small particles. The sludge treatment reduced leaching of Al, Mg, K, Na, and root elongation. Plant did uptake less amount of the cations and P but more Ca with the sludge treatment.

• Journal of Soil and Groundwater Environment
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• v.13 no.4
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• pp.30-39
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• 2008

The uranium removal efficiency of rhizofiltration was investigated in lab scale experiment. Three plants such as sunflower (Helianthus annuus L.), bean (Phaseolus vulgaris var.), and Indian mustard (Brassica juncea (L.) Czern.) were cultivated in artificially contaminated solution by uranium at 30 ${\mu}g$/L and 80 ${\mu}g$/L for 72 hours. The removal efficiencies of three cultivars were calculated from the ICP/MS analysis of uranium mass in solution and the plant. For Helianthus annuus L., more than 80% of initial uranium were removed from the solution and the uranium concentration of residual solution maintained lower than 10 ${\mu}g$/L. For Phaseolus vulgaris var. and Brassica juncea (L.) Czern., their uranium removal efficiencies ranged from 60 to 80%. Even the uranium concentration of solution was higher than 500 ${\mu}g$/L, these cultivars removed more than 70% of initial uranium by rhizofiltration, suggesting that the rhizofiltration has a great capability to remove uranium in the contaminated groundwater. The removal efficiency of rhizofiltration by using Brassica juncea (L.) Czern decreased from 83% to 42% with the increase of pH in solution. From the results of the analysis for the uranium accumulation in plants, 99% of uranium transferred into the plant from solution were accumulated in the root and only 1% of uranium existed in the shoot part (including leaves), suggesting that the cost and the time to treat massive grown plants after the rhizofiltration could be dramatically cut down because only their root parts needs to be treated. Finally, the genuine groundwater having high uranium concentration (81.4 ${\mu}g$/L), sampled from Daejoun area, was used in the experiment. The uranium removal efficiency of Helianthus annuus L. for the real groundwater was higher than 95%, investigating that the rhizofiltration is the very useful method to remediate uranium contaminated groundwater.

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.179-185
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• 2009

This study was carried out to investigate the effect of selenium treatment on the growth of vegetables sprout. Four vegetables, such as cabbage, lettuce, pak-choi and leaf mustard were examined under various selenium treatments (0, 1, 5, 10, 25, 50, 100 mg $L^{-1}$). Seed germinations in cabbage, pak-choi and leaf mustard were significantly inhibited at high concentration of selenium treatment. However, seed germination in lettuce was not much inhibited. Growth characteristics, such as soot length, root length, fresh weight and chlorophyll contents, were not much decreased at 1 mg $L^{-1}$ of selenium and then significantly inhibited with the increase of selenium concentration at above 5 mg $L^{-1}$ in all four vegetables. The selenium content increased linearly with the increase of selenium concentration. At the range of 1 to 25 mg $L^{-1}$ of selenium treatment, selenium contents in vegetables were 0.11 to 1.15 of cabbage, 0.16 to 0.61 of lettuce, 0.13 to 1.31 of pak-choi and 0.14 to 1.13 mg $g^{-1}$dw of leaf mustard, respectively. These results showed that treatment of selenium with the range of 1 to 5 mg $L^{-1}$ could be used to produce the selenium enriched vegetable sprouts.

• Korean Journal of Environmental Agriculture
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• v.26 no.3
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• pp.217-222
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• 2007

To investigate effects of inorganic $(GeO_2)$ and organic (Ge-132) germanium (Ge) on seed germination and on early stage growth of plane and the uptake characteristics, various concentrations (0, 10, 25, 50, 100 mg $L^{-1}$) of Ge to popular vegetables such as leaf mustard, chinese cabbage and pak-choi, respectively, were treated. On seed germination, no significant effect was observed in both inorganic and organic Ge treatments except 100 mg $L^{-1}$ treatment of inorganic Ge. Exogenous inorganic Ge ($10{\sim}100$ mg $L^{-1}$ treatments significantly inhibited the early root elongation growth of all plants. However, slight enhancement of early shoot elongation was detected in low concentrations (10 and 25 mg $L^{-1}$) of Ge in the leaf mustard and chinese cabbage plants. Organic Ge treatments significantly stimulated the 개ot and shoot growth at the 10, 25 and 50 mg $L^{-1}$ treatments. Ge was accumulated linearly in the vegetables as both inorganic and organic Ge concentrations were increased. Interestingly, total contents of Ge in plants with Ge-132 treatments were $2\sim4.5$ times more than those with inorganic Ge treatments in all concentrations. At 25 mg $L^{-1}$ treatment of Ge, contents of Ge in vegetables are following: in leaf mustard, inorganic Ge: 0.37 mg $g^{-1}dw$ and organic Ge: 1.47 mg $g^{-1}dw;$ in the chinese cabbage, inorganic Ge: 0.4 mg $g^{-1}dw$ and organic Ge: 0.86 mg $g^{-1}dw;$ in the pak-choi, inorganic Ge: 0.33 mg $g^{-1}dw$ and organic Ge: 0.70 mg $g^{-1}dw$, respectively. These results showed organic Ge is much better on early stage seedling growth and on germanium accumulation of vegetables than inorganic Ge.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.1
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• pp.67-72
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• 1990

A number of nitrogen-fixing microorganisms were isolated from roots of non-legumious crops and evaluated for their nitrogen-fixing ability on their host crops. The results are summarized as follows: 1. Among nitrogen-fixing isolates obtained from 9 different crops, several isolates showed relatively higher level of acetylene reduction activity on Spinach, Chinese cabbage, Cucumber, mustard and Egg plant. 2. When the isolates were inoculated into 4 host crops, a number of isolates were found to grow well on Spinach, Chinese cabbage and Cucumber, but particularly well on the former. 3. Results of this study suggest that nitrogen-fixing a symbiotic microorganisms are also present and able to grow well on roots of non-legumious crops, and stimulate plant growth by promoting growth and differentiation of roots.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.1
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• pp.152-158
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• 2001

In this study, we determined major foods and food groups which contributed to the intake of folate in korean child-bearing women. Folate content of the major foods was also analyzed. A total of 91 healthy women with childbearing potential participated. They were divided into one of three groups by age; A (15∼24 yrs), B(25∼34 yrs) and C (35∼49 yrs). Food consumption data of the subjects were obtained by the 24-hr recall method. The rate of contribution of each food to the total intake of dietary folate was determined. Seventy major foods for folate were selected from the 178 foods they consumed. Two sets of the 70 foods were collected from different markets. After extracting folates from the foods by heating with a HEPES-CHES buffer, the samples were treated with α-amylase, protease and folate intakes was Korean cabbage kimchi followed by laver, soybean sprout, rice, yolmu kimchi, eggs, lettuce, perilla leaves, sea mustard and radish root. Food items that contributed to folate intake were slightly different among the age groups. Although the folate content of Korean cabbage kimchi was 51.6 ㎍/100g, but due to the high amount of intake, it was ranked the first major food prviding folate. High folate foods containing over 100㎍/100g were laver, corn flakes, spinach, mungbean, sesame, quail's eggs, small radish kimchi, kidney beans, leeks and peanuts. However, based on the serving size, spinach, corn flakes, leeks, sweet potato, pepper leaves, quail's eggs, crown daisy, small radish kimchi and perilla leaves contributed above 50㎍ of folate per serving size.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.543-548
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• 2012

Morphological characters of Baemoochae, xBrassicoraphanus are mostly intermedium of the both parents, Chinese cabbage, Brassica rapa ssp. pekinensis and radish, Raphanus sativus. The upper and lower parts of the leaf resemble the shape of Chinese cabbage and radish, respectively. The midrib of the leaf is round like to that of radish, but very big more than 3 cm in diameter and white in color like that of Chinese cabbage. The root was changed from the swollen type like that of radish to the enlarged taproot like that of the land race of Chinese cabbage after attaining genetical stability. The flower is white. The seed pod is divided into 2 different parts; the upper part is radish and about 4 cm in length and holds 3-4 seeds and the lower part is Chinese cabbage and about 3 cm in length and holds 7-8 seeds. The color of seed is brown, weight per 1.000 seeds is 5.5 g and the number of seeds per mL is 120. The matured plant in the fall season is around 5 kg in weight and outer leaves are very vigorous and stiffly and inner leaves are erect and form a loose head. The leaf and the root contain a high level of sulforaphene which is well known as a functional substance for anti-cancer and anti-super-bacteria. Baemoochae is an amphidiploid and does not have the self incompatibility function. It has a high level of cross compatibility with Chinese cabbage as the female parent, but not the male parent. It is cross incompatible to cabbage, B. oleracea, black mustard, B. nigra and radish. However it is highly compatible to oil seed rape, B. napus, yellow mustard, B. carinata and partial compatible to muatard, B. juncea in the reciprocal cross.

• Restorative Dentistry and Endodontics
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• v.32 no.4
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• pp.356-364
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• 2007

The purpose of this study was to assess the background color-interceptive ability ana opacity of opaque shade composites (Universal composite, Filtek Z350, Charisma, Clearfil ST, Palpaque Estelite, Esthet-X, and Metafil Flo). Twenty four background specimens (diameter 5.5 mm, thickness 3.0 mm) with Root dentin Mustard (Bisco, Schaumburg, IL, USA) were made. The CIE $L^*a^*b^*$ value of background specimens was measured by a spectrophotometer (Spectrolino, GretagMacbeth, Regensdorf, Switzerland). Three specimens in every group were filled on the background specimens. The surface color of samples was measured by a spectrophotometer in 3.0 mm and every thickness to 0.5 mm while grinding. The color difference in the background color along with 3.0 mm specimen gauged the masking effect in each thickness while grinding and polishing. The opacity was calculated in 1 mm thick specimens. The opacity was in the decreasing order of Clearfil ST, Metafil Flo, Filtek Z350, Palpaque Estelite, Universal composite, Charisma, and Esthet-X (p < 0.05). As the thickness get reduced, $L^*$ value showed decreasing, $a^*$ increasing tendency. The surface color difference between pair of the 3.0 mm thick specimen and after grinding in same opaque resin was above 3.3 except Clearfil ST and Metafil Flo. The color difference (${\Delta}E^*$) between pair of background specimen and opaque resin built-up specimen showed more than 10.0 regardless kinds and thickness. The variance in opacity characteristics and color of the opaque composites is dependent upon manufacturer. When using the opaque resin, the optical properties of each material must be considered as well as cavity.