• Asian-Australasian Journal of Animal Sciences
• /
• 제27권10호
• /
• pp.1387-1393
• /
• 2014

This study aimed to estimate genetic correlation between carcass grading and retail productivity traits and to estimate the correlated response on retail productivity traits through selection for carcass grading traits in order to assess the efficacy of indirect selection. Genetic parameters were estimated with the data from 4240 Hanwoo steers using mixed models, and phenotypes included carcass weight (CWT), back fat thickness (BFT), eye muscle area (EMA), marbling (MAR), and estimated lean yield percentage (ELP) as the carcass grading traits, and weight and portion of retail cuts (RCW and RCP), trimmed fats (TFW and TFP) and trimmed bones (TBW and TBP) as the lean productivity traits. The CWT had positive genetic correlations with RCW (0.95) and TFW (0.73), but its genetic correlation with RCP was negligible (0.02). The BFT was negatively correlated with RCP (-0.63), but positively correlated with TFW and TFP (0.77 and 0.70). Genetic correlations of MAR with TFW and TFP were low. Among the carcass grading traits, only EMA was positively correlated with both RCW (0.60) and RCP (0.72). The EMA had a relatively strong negative genetic correlation with TFW (-0.64). The genetic correlation coefficients of ELP with RCP, TFW, and TFP were 0.76, -0.90, and -0.82, respectively. These correlation coefficients suggested that the ELP and EMA might be favorable traits in regulating lean productivity of carcass.

• 대한기계학회논문집B
• /
• 제37권7호
• /
• pp.703-708
• /
• 2013

고관절 전치환술은 대퇴골두와 비구 부분이 손상되어 제 기능을 하지 못하였을 때, 인공 고관절로 대체하는 수술로서 인체에는 많은 양의 절개를 필요로 하며 장기간의 회복시간과 재활시간을 필요로 한다. 최소침습술은 환자에게 흉터와 근육절개를 최소화하고, 회복시간을 단축하여 미관상 흉터가 적어 가장 선호되는 수술 방법이다. 이 논문에서는 최소침습술이 가능한 메커니컬 스템(Mechanical stem)을 기어의 원리를 이용하여 개발하였다. 재치환술을 할 경우에는 스템 전체를 교체할 필요 없이 일부 부품만을 교체하여 수술 시간을 단축할 수 있도록 설계하였으며, 아크릴 소재로 모형을 제작하여 작동에 문제가 없는 것을 확인하였다. 3D Bio-CAD 모델링 기법을 통하여 메커니컬 스템의 기하학적 모델링을 하였다. 실험 대상자의 고관절 부위는 컴퓨터 단층촬영 영상데이터를 기반으로 3차원 재구축을 하였고, 가상 시술 환경 조건에서 몸무게보다 더 과중한 하중을 적용하여 메커니컬 스템의 안전성을 유한요소해석으로 확인하였다.

• 대한본초학회지
• /
• 제23권4호
• /
• pp.9-19
• /
• 2008

Objectives: In this study the authors investigated effects of the ethanolic extract of Rhodjola Rosea(HKC) on fatigue and hypothalamic IEG expression in rat forced swimming(FS) model. Methods: Sprague-Dawley rats were administered HKC extract(25 mg/100g, p.o.) for 3 days prior to FS, some rats underwent 10 min FS and others exhaustive forced swimming(EFS). In addition, other rats were administered extract at different times after EFS over 3 consecutive days. Results: When HKC administered before 10 mins of FS, serum actate dehydrogenase(LDH) and creatine phosphokinase(CPK) activities were significantly lower than control group. When HKC administered prior to EFS, blood lactate was significantly lower versus control group. When HKC was administered after EFS, blood lactate(at 6 and 24 hours after EFS) were significantly lower and serum LDH, CPK activities(at 24 hours after EFS) were significantly lower versus control group. When HKC was administered after EFS, c-Fos positive neurons in hypothalamic periventricular area(PVA), medial part(mPVN) and anterior hypothalamic nucleus caudal part(AHC) were significantly lower at 24 hours after EFS than in control group. HSP-72 positive neuron numbers in hypothalamus were significantly lower at 24 hours after EFS than in control group. Finally, when HKC was administered prior to 10 mins FS, HIF-$1{\alpha}$ expression in the gastrocnemius muscle was significantly increased. Conclusions: These results suggest that HKC extract has an anti-fatigue effect, and it reduces neuronal cell stress responses induced by physical stress by having a beneficial effect on lactate metabolism.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
• /
• pp.411.1-411.1
• /
• 2014

This study evaluates the utility of an antibacterial microneedle composed of green tea extract (GT) and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ${\sim}60mm^2$ with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate were sustained for 72 h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles are not generally cytotoxic to chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24 h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida) and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth in the infected skin wound sites and improved skin wound healing process in rat model.

• Preventive Nutrition and Food Science
• /
• 제7권2호
• /
• pp.113-118
• /
• 2002

Alpha-lipoic acid is a known hypoglycemic agent that may be useful in the treatment of diabetes. The objective of this study was to investigate the fate of glucose in isolated muscles incubated with lipoic acid by determining its direct effects on specific metabolic and signaling pathways. Soleus muscles from healthy rats were incubated with lipoic acid in the absence or presence of insulin. Glucose transport, glycogen synthesis, glucose oxidation and lipid synthesis were determined and affects on major pathways associated with insulin signaling were evaluated. Glucose transport was not significantly altered by the addition of lipoic acid to the incubation medium. However, lipoic acid decreased glycogen synthesis in comparison to controls. Glucose oxidation was moderately increased while de-novo lipid synthesis from glucose was inhibited. Wortmannin repressed insulin stimulation of glucose incorporation into glycogen, an effect that was augmented by the combined treatment of wortmannin and lipoic acid. Basal and insulin-stimulated serine phosphorylation of Akt was not changed by the addition of lipoic acid to the incubation medium. These data show that in this in vitro model, lipoic acid did not significantly affect glucose uptake but dramatically modified pathways of glucose metabolism within muscle tissue.

• 한국발생생물학회지:발생과생식
• /
• 제15권1호
• /
• pp.53-59
• /
• 2011

Sex steroid hormones are key molecules to prepare the decidual response and their levels are important in this process. Imbalances of the levels of steroid hormones are cause of implantation failure and other diseases including physical weakness. Androgen replacement therapy or selective androgen receptor modulator are used to overcome various diseases but long-term use may cause of side effects. In previous report, it is suggested that the steroid hormonal complexes derived from pig enhance the proliferation of satellite cell. Therefore, to evaluate the possible usage of steroid hormonal complex derived from pig testis (tS-C), the effects of tS-C on uterine response were studied using the model of artificial decidua. tS-C did not disturb the rhythmical estrus cycle. Artificial-induced decidual response was normally induced in tS-C administered mice. The histological characters of the decidua of tS-C administered mice were not different from the vehicle. The expression patterns of molecular markers of decidua were not different between vehicle and tS-C group. Collectively these results suggested that tS-C does not disturb the uterine responsibility to the embryo. In addition, our results suggested that tS-C can be applied to overcome the various problems such as loss of muscle mass and anemia.

• Journal of Animal Science and Technology
• /
• 제55권6호
• /
• pp.501-508
• /
• 2013

This study was conducted to determine genetic correlations among carcass traits measured by ultrasound and real carcass measurements and to estimate indirect selection responses for real carcass traits based on ultrasound measurements in Hanwoo cows. To accomplish this, 22,080 ultrasound measurement records from 17,926 cows collected from 2001 to 2012 and 11,907 carcass records obtained from fattened cattle from 2008 to 2012 were used. Genetic parameters were estimated based on eye muscle area (EMA), backfat thickness (BF) and marbling score (MS) measured by ultrasound-scanning of live cows and using the official technique on chilled bovine half-carcasses after slaughtering. Heritability and genetic correlation for carcass traits were estimated using a mixed model equation that consisted of environmental effects as fixed parameters and additive genetic effects and residual effects as random parameters, assuming that traits were different between ultrasound and carcass measurements. This statistical method was applied to the average information restricted maximum likelihood method. The heritability of EMA, BF and MS measured by ultrasound were 0.33, 0.61 and 0.46, respectively, while the heritability estimates of the corresponding traits based on carcass measurements were 0.29, 0.40 and 0.38, respectively and the genetic correlation between ultrasound and carcass traits for EMA, BF and MS were 0.41, 0.78 and 0.67, respectively. The genetic correlation between ultrasound and carcass traits was highly positive. Additionally, the selection response for marbling score was estimated to be 0.42 per generation if the cows were selected based on the ultrasound scan marbling score with an assumed selection intensity of 0.8. Overall, these results indicate that the ultrasound scan technique would be applicable to judging cow selection for genetically improved meat quality.

• 대한음성언어의학회:학술대회논문집
• /
• 대한음성언어의학회 2003년도 제19회 학술대회
• /
• pp.181-181
• /
• 2003

Objectives : Vocal fold augmentation using injectable material is an easy and simple operation. This study is to evaluate the histology of minced and injected autologous auricular cartilage and fat graft in the augmentation of unilateral vocal fold paralysis using a canine model for two years. Study Design : A prospective study with the contralateral side of the larynx used as the control Methods : Twelve dogs were operated. At first, a piece of auricular cartilage was harvested from ear and minced into tiny chips with a scalpel and scissors. And also, a piece of fat tissue was harvested from inguinal area and minced into tiny chips with a scalpel and scissors. The minced cartilage and fat-paste (0.2ml) was injected using a pressure syringe into the paralyzed thyroarytenoid muscle under direct laryngoscopy. Two animals were sacrificed at 3 days, three at 3 weeks. two at 3 months. one at 6 months, one at 12 months, three at 24 months. Each dog underwent laryngectomy and serial coronal sections of paraffin blocks from the posterior part of the vocal fold were made. Result : There was no significant complication perioperatively and during follow-up. There was acute inflammatory findings in the graft at 3 days and 3 weeks. Only a very small proportion of the injected cartilage was absorbed due to the degenerative change and the overall volume was preserved even when the cells died out. The injected cartilage remained in the larynx until 24 months. Conclusion : The autologous cartilage implant using auricular cartilage was the ideal vocal cord augmentative material for the treatment of glottic incompetence.

• 대한음성언어의학회:학술대회논문집
• /
• 대한음성언어의학회 2003년도 제19회 학술대회
• /
• pp.180-180
• /
• 2003

Objectives : Vocal fold augmentation by injectable material under direct visual control is an easy and simple operation. However, when autologous fat or bovine collagen is used, resorption creates a problem. And autologous fascia is debating about absorption now days. This study is to evaluate the histology of minced and injected autologous auricular cartilage and fat graft in the augmentation of unilateral vocal fold paralysis using a canine model. Methods : Nine dogs were operated. At first, a piece of auricular cartilage was harvested from ear and minced into tiny chips with a scalpel. And also, a piece of fat tissue was harvested from inguinal area and minced into tiny chips with a scalpel. Cutting off a section of the recurrent nerve paralyzed the right vocal fold. The minced cartilage and fat-paste (0.2ml) was injected using a pressure syringe into the paralyzed thyroarytenoid muscle under direct laryngoscopy. Two animals were sacrificed at 3 days, three at 3 weeks, two at 3 months, one at 6 months, one at 12 months. Each dog underwent laryngectomy and serial coronal sections of paraffin blocks from the posterior part of the vocal fold were made. Results : There was no significant complication perioperatively and during follow-up. There was acute inflammatory findings in the graft at 3 days and 3 weeks. The injected cartilage remained in the larynx until 12 months. Conclusion : The autologous auricular cartilage graft is well tolerated and may be very effective material for volumetric augmentation on paralyzed vocal cord.

• 대한후두음성언어의학회지
• /
• 제10권2호
• /
• pp.119-123
• /
• 1999

Background and Objectives : Conditions such as muscle atrophy, stretching of strap muscles, and continued craniofacial growth factors have been cited as contributing to the changes observed in the vocal tract structure and function in elderly speakers. The purpose of the present study is to compare F$_1$ and F$_2$ frequency levels in elderly and young adult male and female speakers producing a series of vowels ranging from high-front to low-back placement. Material and Methods : The subjects were two groups of young adults(10 males, 10 females, mean age 21 years old range 19-24 years) and two groups of elderly speakers(10 males, 10 females, mean age 67 years : range 60-84 years). Each subject participated in speech pathologist to be a speaker of unimpared standard Korean. The headphone was positioned 2 cm from the speakers lips. Each speaker sustained the five vowels for 5 s. Formant frequency measures were obtained from an analysis of linear predictive coding in CSL model 4300B(Kay co). Results : Repeated measure AVOVA procedures were completed on the $F_1$ and $F_2$ data for the male and female speakers. $F_2$ formant frequency levels were proven to be significantly lower fir elderly speakers. Conclusions : We presume $F_2$ vocal cavity(from the point of tongue constriction to lip) lengthening in elderly speakers. The research designed to observe dynamic speech production more directly will be needed.