• Molecules and Cells
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• 제41권8호
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• pp.799-807
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• 2018

Emerging evidence has suggested that cellular crosstalk between RNF168 and poly(ADP-ribose) polymerase 1 (PARP1) contributes to the precise control of the DNA damage response (DDR). However, the direct and reciprocal functional link between them remains unclear. In this report, we identified that RNF168 ubiquitinates PARP1 via direct interaction and accelerates PARP1 degradation in the presence of poly (ADP-ribose) (PAR) chains, metabolites of activated PARP1. Through mass spectrometric analysis, we revealed that RNF168 ubiquitinated multiple lysine residues on PARP1 via K48-linked ubiquitin chain formation. Consistent with this, micro-irradiation-induced PARP1 accumulation at damaged chromatin was significantly increased by knockdown of endogenous RNF168. In addition, it was confirmed that abnormal changes of HR and HNEJ due to knockdown of RNF168 were restored by overexpression of WT RNF168 but not by reintroduction of mutants lacking E3 ligase activity or PAR binding ability. The comet assay also revealed that both PAR-binding and ubiquitin-conjugation activities are indispensable for the RNF168-mediated DNA repair process. Taken together, our results suggest that RNF168 acts as a counterpart of PARP1 in DDR and regulates the HR/NHEJ repair processes through the ubiquitination of PARP1.

• Journal of Korea Trade
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• 제25권3호
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• pp.65-86
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• 2021

Purpose - This paper empirically investigates the relationship between the corporate value chain and performance of non- financial businesses of South Korean stock market companies. It aims to explore the evidence that can be used to infer the relationship between value chains and corporate performance in the case of firms forming a value chain with other companies with the means of an equity investment or a special business relationship. Design/methodology - Non-financial corporations listed from 2011 to 2017 on the securities market of South Korea are analyzed. The data used for analysis are found for transactions with the related party by year for all the corporations of non-financial industries in the securities market. Multiple analysis attempts are conducted including the relationship between the value chain and productivity, corporate value, risk-adjusted corporate value, and mediation effects of productivity. The empirical model employs sixteen variables including the value chain index which identifies its impact on various aspects of business performances. Findings - The results of this study clearly supports the phenomenon that corporate productivity and value are enhanced when the corporation expands its value chain established with domestic related firms and overseas companies. Such a positive effect is statistically significant even after the possible risk factors that accompany the expansion of value chain were considered, and productivity plays the role as a medicating variable in the effect of the value chain on the corporation values. Originality/value - The findings of this study confirms that domestic companies' expansion of their value chain centered on the related firms overseas that helped them in terms of the maximization of their productivity and corporate values. This study shows that Korean government's policy on expanding the corporate GVC can enhance the productivity and value of firms. The expansion of value chain and its impact on business performance has not been explored thoroughly, although it is getting more and more important in the global trade operation.

• 융합정보논문지
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• 제9권1호
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• pp.86-91
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• 2019

2008년 금융위기 사태가 발생한 이후 금융 시스템의 중앙집권의 위험성을 인지하면서 시작된 것이 블록체인이다. 2008년 시작점으로부터 지금까지 블록체인은 비트코인이라는 블록체인 기술 기반 암호화폐란 엄청난 혁신을 시작으로 많은 관심과 기술의 발전이 이루어지고 있는 상황이다. 하지만 이 또한 시간이 지나면서 취약점이 발견되었고 피해 사례 또한 자주 일어나고 있다. 그러나 현재 21세기 4차 산업에 있어 핵심 기술이며 앞으로도 많은 발전과 이를 기반으로 한 기술들이 다수 출현할 것으로 예상된다. 따라서 본 논문은 암호화폐 거래에서의 취약점을 분석하고 새로운 거래 프로토콜을 제안한다. 제안하는 거래 프로토콜은 거래가 끝나는 시점에서 한 번 더 인증을 하게 되고 이러한 인증으로 거래가 성사되는 방식이다. 거래 취약점에 대한 내부적 보안이 아닌 거래 과정에서 사용자들 간의 보안요소를 추가하여 대응방안을 제안한다.

• Journal of Microbiology and Biotechnology
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• 제29권1호
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• pp.141-150
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• 2019

The spatial landmark protein Bud8 plays a crucial role in bipolar budding in the budding yeast Saccharomyces cerevisiae. The unconventional yeast Yarrowia lipolytica can also bud in a bipolar pattern, but is evolutionarily distant from S. cerevisiae. It encodes the protein YALI0F12738p, which shares the highest amino acid sequence homology with S. cerevisiae Bud8, sharing a conserved transmembrane domain at the C-terminus. Therefore, we named it YlBud8. Deletion of YlBud8 in Y. lipolytica causes cellular separation defects, resulting in budded cells remaining linked with one another as cell chains or multiple buds from a single cell, which suggests that YlBud8 may play an important role in cell separation, which is distinct from the function of Bud8 in S. cerevisiae. We also show that the YlBud8-GFP fusion protein is located at the cell membrane and enriched in the bud cortex, which would be consistent with a role in the regulation of cell separation. The coiled-coil domain at the N-terminus of YlBud8 is important to the correct localization and function of YlBud8, as truncated proteins that do not contain the coiled-coil domain cannot rescue the defects observed in $Ylbud8{\Delta}$. This finding suggests that a new signaling pathway controlled by YlBud8 via regulation of cell separation may exist in Y. lipolytica.

• International Journal of Computer Science & Network Security
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• 제22권12호
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• pp.37-50
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• 2022

Modern supply chains include multiple activities from collecting raw materials to transferring final products. These activities involve many parties who share a huge amount of valuable data, which makes managing supply chain systems a challenging task. Current supply chain management (SCM) systems adopt digital technologies such as the Internet of Things (IoT) and blockchain for optimization purposes. Although these technologies can significantly enhance SCM systems, they have their own limitations that directly affect SCM systems. Security, performance, and scalability are essential components of SCM systems. Yet, confidentiality and scalability are one of blockchain's main limitations. Moreover, IoT devices are lightweight and have limited power and storage. These limitations should be considered when developing blockchain-based IoT-SCM systems. In this paper, the requirements of efficient supply chain systems are analyzed and the role of both IoT and blockchain technologies in providing each requirement are discussed. The limitations of blockchain and the challenges of IoT integration are investigated. The limitations of current literature in the same field are identified, and a secure and scalable blockchain-based IoT-SCM system is proposed. The proposed solution employs a Hyperledger fabric blockchain platform and tackles confidentiality by implementing private data collection to achieve confidentiality without decreasing performance. Moreover, the proposed framework integrates IoT data to stream live data without consuming its limited resources and implements a dualstorge model to support supply chain scalability. The proposed framework is evaluated in terms of security, throughput, and latency. The results demonstrate that the proposed framework maintains confidentiality, integrity, and availability of on-chain and off-chain supply chain data. It achieved better performance through 31.2% and 18% increases in read operation throughput and write operation throughput, respectively. Furthermore, it decreased the write operation latency by 83.3%.

• 영어영문학
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• 제56권6호
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• pp.1295-1310
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• 2010

In her "After Language Poetry: Innovation and Its Theoretical Discontents" in Contemporary Poetics (2007), Marjorie Perloff spotted Steve McCaffery's and Lyn Hejinian's points of reference and opacity/transparency in poetic language, and theorizes in her perspicacious insights that poetic language is not a window, to be seen through, a transparent glass pointing to something outside it, but a system of signs with its own semiological interconnectedness. Providing a critique and contextualizing Perloff's argument, the purpose of this paper is to introduce a topological model for poetry, language, and theory and further to elaborate the relation between the theory and the practice of language poetry in terms of "the revolution of language." Jacques Lacan's poetics of knowledge and of the topology of the mind, in particular, that of "extimacy," can articulate the way how language poetry problematizes the opposition between inside and outside in the substance of language itself. In fact, as signifiers always refer not to things, but to other signifiers, signifiers becomes unconscious, and can say more than they actually says. The original signifiers become unconscious through the process of repression which makes a structure of multiple and polyphonic signifying chains. Language poets use this polyphonic language of the Other at Freudian "Another Scene" and Lacan's "Other." When the reader participates the constructive meanings, the locus of the language writing transforms itself into that of the Other which becomes the open field of language. The language poet can even manage to put himself in the between-the-two, a strange place, the place of the dream and of the Unheimlichkeit (uncanny), and suture between "the outer skin of the interior" and "the inner skin of the exterior" of the impossible real of definite meaning. The objective goal of the evacuation of meaning is all the same the first aspect suggested by the aims of the experimentalism by the language poetry. The open linguistic fields of the language poetry, then, will be supplemented by The Freudian "unconscious" processes of dreams, free associations, slips of tongue, and symptoms which are composed of this polyphonic language. These fields can be properly excavated by the methods and topological mapping of the poetics of extimacy and of the klein bottle.

• 디지털융복합연구
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• 제20권3호
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• pp.325-331
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• 2022

에지 네트워크 환경에서 IoT 장치가 다양하게 활용되면서 IoT 장치에서 수집되는 정보들을 여러 응용 분야에서 활용하는 연구들이 다양하게 진행되고 있다. 그러나, 네트워크 환경(간섭, 전파방해 등)에 따라 수집되는 IoT 데이터들이 누락 또는 오류가 발생하는 상황이 빈번해지면서 정확한 IoT 데이터들을 바로 적용하기가 쉽지 않은 상황이다. 본 논문에서는 에지 네트워크 환경에서 수집되는 IoT 데이터들의 오류를 줄이기 위해서 IoT 데이터의 서명 값을 랜덤하게 생성하여 비트 형태로 보안 정보(Security Information, SI) 값만을 IoT 데이터들에 각각 할당함으로써 IoT 데이터의 신뢰성을 보장하는 관리 기법을 제안한다. 제안 기법은 IoT 장치로부터 수집되는 데이터들을 비대칭적으로 서로 연계 처리하도록 다중 해쉬 체인을 적용하여 IoT 데이터를 블록체인으로 묶는다. 이때, 블록 체인화된 IoT 데이터들은 딥러닝 기반으로 상관관계 지수에 따라 가중치를 적용한 확률 함수를 사용한다. 또한, IoT 데이터의 무결성과 처리 비용을 낮추기 위해서 제안 기법은 그룹화된 IoT 데이터를 n-계층 구조로 확장 운영 가능하다.

• Journal of Periodontal and Implant Science
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• 제29권3호
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• pp.507-519
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• 1999

The ultimate objective of periodontal treatment is to stop disease progression and to regenerate destroyed periodontal tissues and thereby regain normal function. Growth factors are naturally found polypetides which stimulate many cellular activities pertaining to wound healing by acting as signal molecule in controlling cell movement, proliferation, and matrix production. Platelet derived growth factor (PDGF) is 28,000-35,000 Da molecular weight dimeric protein with 2 long positively charged polypeptide chains connected by sulfide bonds. The purpose of this study is to evaluate histologically the initial guided tissue regeneration in a periodontal defect f a beagle dog treated with a biodegradable membrane formed with polylactic acid (poly-L-lactic acid) and polyglycolic acid loaded with 200ng/$cm^2$ platelet derived growth factor. 2 beagle dogs were used in he experiment. $5mm{\times}6mm$ alveolar bone defect was formed in upper and lower canines and third premolars and a reference notch was placed. PDGF-BB non-containing membrane was used as control. Each defect was randomly assigned to the test roup or the control group. The dogs were sacrificed 3 weeks after membrane placement. Toluidine blue and multiple staining was done for histological analysis. In the 3 week specimen in the control group, no new one formation could be seen. Small amount f bone resorption below the notch could be seen. In the notch, loose connective tissue with infiltration of inflammatory cells could be seen. Also thin discontinuous new cementum could be seen and the membrane still retained its structure. Where PDGF-BB containing membrane was used, new bone formation could be seen in the notch at weeks and also continuous thin cementum could be seen. PDL cells were observed between new bone and new cementum and some were attached to bone and cementum. These results suggest that new bone and cementum formation seen when PDGF-BB loaded membrane was used was due to inhibition of downgrowth of epithelial cells and also due to continuous release of the growth factor. Further study on the resorption characteristics of the membrane nd the release characteristics of the PDGF-BB is necessary. Also, development of a membrane easier to use clinically is necessary.

• 정보처리학회논문지C
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• 제12C권5호
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• pp.623-632
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• 2005

애드혹 네트워크는 기존의 유무선 네트워크의 고정된 기반시설(infrastructure) 없이 이동 호스트들만으로 구성된 무선 환경의 네트워크이다. 애드혹 네트워크의 기본 특성, 즉, 링크의 불안정성, 각 노드의 물리적 보호의 한계, 노드간 연결의 산재성, 토폴로지의 동적인 변화 뿐 아니라 악의적인 노드의 활동으로 인해 라우팅 보안에 대한 위험성은 매우 높다 따라서 본 논문에서는 애드혹 네트워크에서 경로 탐색이나 설정 과정중 악의적인 노드가 라우팅 메시지를 변조, 위조하거나 다른 노드를 가장하여 잘못된 라우팅 정보를 네트워크에 주입시키는 공격을 방지하기 위하여 일방향 해쉬 함수를 기초로 한 one-time 전자 서명을 이용한 라우팅 보안 메커니즘을 제안한다. 제안하는 메커니즘에서 노드들은 라우팅 메시지를 서명하기 위하여 공개키 요소의 첫 세트를 반복적으로 해쉬 함수에 적용함으로써 해쉬 체인을 생성하고, 생성된 해쉬 체인으로부터 공개키 요소들을 여러 세트 유도하여 해쉬 테이블을 생성한다. 해쉬 테이블 생성 후, 노드들은 자신의 공개키 요소를 다른 노드들에게 공표하고 라우팅 메시지를 전송할 경우 one-time 전자 서명을 포함한다. 이러한 one-time 전자 서명은 라우팅 메시지를 인증하고 메시지에 무결성을 제공한다. 제안하는 라우팅 보안 메커니즘은 이동성이 높은 네트워크 환경에서는 보안을 고려하지 않은 라우팅 메커니즘에 비해 라우팅 오버헤드가 좀더 높아지지만, 경로를 탐색하고 설정하는 과정에서 악의적인 노드의 공격에 대하여 훨씬 높은 안전성을 제공함을 시뮬레이션을 통해서 확인할 수 있다.

• Korean Chemical Engineering Research
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• 제46권2호
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• pp.316-329
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• 2008

최근의 급변하는 시장 상황의 변화와 제품의 수요에 대한 다양한 요구는 회분식 공정에 의한 다품종 소량생산으로의 전환을 가져오게 하였다. 이러한 회분식 공정은 주로 정밀 화학 관련 제품들인 의약품, 생화학 제품, 농약, 고분자 소재 등의 생산에 사용되어 왔지만, 근래에는 윤활유, 섬유, 석유 화학, 식품 같은 제품의 생산에도 널리 적용되고 있다. 그러나 회분식 공정은 원료의 공급, 제품의 가격 등과 같은 불확실 변수에 의한 조업의 변화가 자주 발생하는 단점이 있다. 이러한 조업의 변화는 조업시간의 변동과 각 부분별 예측량이 달라져 시장 경쟁력을 잃게 된다. 이에 공급망 상에 위치한 각 부서별, 기업별 협력과 조정을 통한 총체적 관점에서의 최적화를 추구하는 공급사슬 관리에 관한 관심이 고도되고 있다. 이에 본 논문에서는 회분식 공정에 공급사슬 관리 기법을 도입하여 원자재의 구매에서부터 분배에 이르는 전과정에 대한 총체적인 최적해를 찾고 각 공급사슬간의 영향력을 조사, 분석하였다. 또한 본 논문에서는 생산계획과 상세일정계획 모델의 통합과 수요에 대한 단계별 예측을 통해 시장 변화와 불확실 변수(uncertainty)에 대한 적절한 대응방안을 모색하여, 회분식 공정에서의 공급사슬 관리 모델을 개발하였다. 이와 함께 각각의 공급사슬간 인터페이스를 통하여 정보와 물류의 통합이 이루어지게 하여, 실제 주문과 수요의 변화에 대하여 생산계획, 구매계획, 일정계획 및 분배계획을 수립하여 안정적인 공급이 이루어지게 하였다.