• 한국응용과학기술학회지
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• 제32권1호
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• pp.165-169
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• 2015

N-propyl-N,N-dimethylethanolamine의 용액을 만들기 위해 산성 조건 하에서 6분간 초음파처리하여 제조하였다. N-propyl-N,N-dimethylethanolamine 의 촉매 가수분해는 온도 $30{\sim}55^{\circ}C$에서 uni-lamellar vesicle과 uni-lamellar와 multi-lamellar가 뭉쳐진 혼합물에서 연구되었다. 이들의 차이는 촉매효과에 대해 multi-lamellar보다 uni-lamellar에서 영향이 크게 나타났다. Vesicle의 상전이 온도는 $37{\sim}44^{\circ}C$이며, multi-lamellar의 분자 입자의 크기는 uni-lamellar의 입자의 크기보다 크게 나타 났다.

• 한국응용과학기술학회지
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• 제36권2호
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• pp.592-599
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• 2019

This study is to stabilize insoluble and unstable active ingredient which is Idebenone (INCI name: hydroxydecyl ubiquinone) in a multi-lamellar vesicle (MLV) and to stabilize it in the skin care cosmetics. Idebenone is good effective raw material in the treatment of Alzheimer's disease in the medical field and a powerful antioxidant in dermatology. It is well known as a substance that inhibits the formation of melanin and cleans the skin pigment. However, it did not dissolve in any solvent and it was difficult to apply in cosmetic applications. Niosome vesicle was able to develop a nano-particle by making a multi-layer of idebenone encapsulated with a nonionic surfactant, hydrogenated lecithin and glycine soja (soybean) sterols and passing it through a high pressure microfluidizer. Idebenone niosome vesicle (INV) has been developed to have the ability to dissolve transparently in water and to promote transdermal penetration. The appearance of the INV was a yellowish liquid having specific odor, and the particle size distribution of INV was about 10~80 nm. The pH was 5~8 (mean=6.8). This capsulation with idebenone was stored in a $45^{\circ}C$ incubator for 3 months and its stability was observed and quantitatively measured by HPLC. As a result, the stability of the sample encapsulated in the niosome vesicle (97.5%) was about 66.3% higher than that of the non-capsule sample of 32.5%. Idebenone 1% INV was used for the efficacy test and clinical trial evaluation as follows. The anti-oxidative activity of INV was 38.2%, which was superior to that of 12.8% tocopherol (control). The melanin-reducing effect of B16 melanoma cells was better than INV (17.4%) and Albutin (control) (9.6%). Pro-collagen synthesis rate was 128.2% for INV and 89.3% for tocopherol (control). The skin moisturizing effect was 15.5% better than the placebo sample. The elasticity effect was 9.7% better than the placebo sample. As an application field, INV containing 1% of idebenone is expected to be able to develop various functional cosmetic formulations such as skin toner, ampoule essence, cream, eye cream and sunblock cream. In addition, it is expected that this encapsulated material will be widely applicable to emulsifying agents for skin use in the pharmaceutical industry as well as the cosmetics industry.

• 한국응용과학기술학회지
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• 제25권2호
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• pp.205-210
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• 2008

The transformation of the liquid crystal complex made by binding of anionic surfactant, sodium dodecyl sulfate (SDS), into high charge density cationic polymer, the homopolymer of diallyldimethylammonium chloride (PDADMAC) was induced by adding of nonionic surfactants and investigated by means of microscopy and FE.SEM. Among nonionic surfactants in this experiments polyethylene glycol (3 mol) ether of lauryl alcohol (laureth-3) made variation in the complex. The laureth-3 transformed the complex into spherulite vesicle with the size of ca.$100{\mu}m$. This change increased the viscosity and the turbidity of the solution phase separated originally. Microscope showed that they are spherulite particles and polarized microscope suggested they are multi.lamellar liquid crystals. FE-SEM also proved that explicitly.

• 한국응용과학기술학회지
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• 제17권4호
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• pp.248-256
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• 2000

We investigated the property of formation of mono-vesicle(designated nano-some) with using of the combined co-emulsifiers and phospholipid. Nano-some was prepared with hydrogenated lecithin(HL) and diethanolamine cetyl phosphate(DEA-CP) by swelling reaction. Kojic acid and kojic dipalmitate could be made stabilization by nano-some system using microfluidizer(MF). Nano-some has a good affinity to skin by means of this system. The composition was compounded by 2% of hydrogenated lecithin (phosphatidyl choline contained with 75%, 0.5% of DEA-CP and 0.5% of diglyceryl dioleate (DGDO). To make nano-some, several conditions of MF have to be considered as follows. The optimum pH was 6.0. The pressure was 10,000psi and passage temperature was at $306^{\circ}C$. The nano-some base was passed to homogenize continually 3 times through MF. The Particle size distributions of the vesicles were with in $57{\sim}75.7nm$(mean 66nm) by measuring the Zetasizer-3000. Zeta potential of vesicles with 3 times passage through MF was -24.8mV. Formations for nano-some vesicle certificated photograph by scanning electric magnification (SEM). Stability of nano-some was very good for 6months. The turbidity was very good transparency compared nano-some with liposome. It was formed the mono vesicle in the opposite direction to be formed the multi-lamellar vesicle of liposome.

• 대한화장품학회지
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• 제27권1호
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• pp.29-42
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• 2001

We had prepared MLV liposome with Hibiscus Esculentus Ext.(HEE) which have fluorescent light in order to evaluate its percutaneous absorption about hairless rat skin. Then we investigated particle size of MLV using confocal laser scanning microscope(CLSM) and transmission electron microscope(TEM), respectively. Stability of MLV liposome and penetration of MLV liposome to hairless rat skin was measured by CLSM. As a result of experiments, MLV was globular shape and the rage of particle size was 0.3-0.5$\mu\textrm{m}$ mostly. Cream-type MLV had high stability comparatively. When we treated with MLV to rat skin, skin penetration was enhanced, especially, the optimum concentration of MLV on penetration to rat skin was 10%. Optimum penetration time was 6hr-12hr. And MLV-type HEE was more effective on percutaneous absorption than HEE-cream or liposome-type HEE.

• 한국응용과학기술학회지
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• 제19권1호
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• pp.10-18
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• 2002

In this study, it should be mentioned that Lipid-LCG can be prepared with the main compound of hydrogenated lecithin in oil-in water emulsion. The results of its physical property and stability are as follows. First, the best suitable compositions of Lipid-LCG are made from 4.0wt% of the hydrogenated lecithin, 4.0wt% of cetostearyl alcohol as emulsifier and gelling agent, 3.0wt% of butylene glycol and 2.0wt% glycerin as moisturizers, 3.0wt% of cyclomethicone, 3.0wt% of isononyl-isononanoate, 3.0wt% of capric/caprylic triglycerides, 3.0wt% of macadamia oil as emollients. Second, As the optimum conditions to form Lipid-LCG, which figured out 6.0 ${\pm}$ 1.0 for pH level, 32kg/mm, min for hardness to make a .essence to be formed the ternary phase of liquid crystal(multi-lamellar type). Third, as the analytical result of this system, it obtained that particle size is $1{\sim}8{\mu}m$ level, and is certified with it at 400 and 1,000 magnifications by microscope. The stability of Lipid-LCG is very stable on condition of a low temperature ($4^{\circ}C$), a room temperature ($25^{\circ}C$) and a high temperature ($40^{\circ}C$), which is not to be split in for a long time(for 3-month). We produced our own moisturizing essence, which has a good affinity to skin by means of this system.

• Applied Microscopy
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• 제6권1호
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• pp.21-32
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• 1976

Rhizopus nigricans, Aspergillus niger, Aspergillus ochraceus의 균계 Agricus bisporus, Rhizopogon rubescens의 자실층균계 및 담자기세포, Marchantia polymorpha, Pogonatum inflexum, Pteridium aqiulium 의 동화조직, Pinus densiflora, Ginkgo biloba, Panax ginseng 의 엽육조직의 소편을 glutaralehyde-paraformalehyde 및 $OsO_4$에 고정하여 다소포체 및 세포질용해체와 관련된 다양상인 막성구조의 기원과 기능에 관하여 전 자현미경적으로 추구하였다. Rhizopus nigricans, Aspergillus niger, A. ochraceus, Agricus bisporus, Rhizopogon rubescens 의 동심원상인 다층막, 미에린一소포-관상구조의 복합체, 평행다층막구조등은 원형질막에서 유래된다. Marchantia polymorpha, Pogonatum inflexum, Pteridium aquilinum, Pinus densiflora, Ginkgo biloba, Panax ginseng 에 있어서 pinocytotic vesicles 외에 다양상안 막성구조는 원형질막과 세포질안의 무과립성 소포체에서 유래된다. 원형질막에서 유래되는 것은 다소포체인 것과 미에린 양구조인 것으로 구분되고, 이것은 2차액포를 형성하거나 또는 1차액포안으로 유리되어 퇴화한다. 세포질안의 소포체가 세포질의 일부를 포위하고 그 내부에 막증식과 더불어 세포질이 감소되면서 다층막구조로 변하고, 이것은 액포안으로 유리되어 퇴화한다. 세포질의 소포체에서 유래되는 것은 액포분화와 밀접한 관계가 있는 세포질분리체 또는 세포용해체인 것으로 믿어진다.