• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.319-324
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• 2006

Although the sera used in animal cell culture media provide the macromolecules, nutrients, hormones, and growth factors necessary to support cell growth, it could also be an obstacle to the production of recombinant proteins in animal cell culture systems used in many sectors of the biotechnology industry. For this reason, many research groups, including our laboratory, have been trying to develop serum-free media (SFM) or serum-supplemented media (SSM) for special or multi-purpose cell lines. The Chinese hamster ovary (CHO) cell, for example, is frequently used to produce proteins and is especially valuable in the large-scale production of pharmaceutically important proteins, yet information about its genome is lacking. Also, SFMs have only been evaluated by comparing growth patterns for cells grown in SFMs with those grown in SSM or by measuring the titer of the target protein obtained from cells grown in each type of medium. These are not reliable methods of obtaining the type of information needed to determine whether an SFM should be replaced with an SSM. We carried out a cDNA microarray analysis to evaluate MED-3, an SFM developed in our laboratory, as a CHO culture medium When CHO cells were cultured in MED-3 instead of an SSM, several genes associated with cell growth were down-regulated, although this change diminished over time. We found that the insulin-like growth factor (IGF) gene was representative of the proteins that were down-regulated in cells cultured in MED-3. When several key supplements - including insulin, transferrin, ethanolamine, and selenium - were removed from MED-3, the IGF expression was consistently down- regulated and cell growth decreased proportionately. Based on these results, we concluded that when an SFM is used as a culture medium, it is important to supplement it with substances that can help the cells maintain a high level of IGF expression. The data presented in this study, therefore, might provide useful information for the design and development of SFM or SSM, as well as for the design of genome-based studies of CHO cells to determine how they can be used optimally for protein production in pharmaceutical and biomedical research.

• Journal of Families and Better Life
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• v.28 no.5
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• pp.167-181
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• 2010

This research was designed to explore the meaning of housing among Korean Chinese in Harbin, China. In particular, the meaning of housing was examined by using the pathway approach. Utilizing qualitative research methods, this study administered the in-depth interview on the oral history of an individual life, and the 5 elderly persons in their 60s and 70s participated in the individualized interviews that were conducted from May 28 to 31 in 2010. The main findings of meaning of housing were as follows; 1. Similarly to the meaning of housing in 1970s and 1980s in Korea, house was viewed as both a shelter for family members and relatives and a place for their comfort. 2. Prior to multi-story residences, Harbin had only 3 different forms of single-story houses available; Chinese style with Kang and soil room(地室), Korean style with 'Ondol', and Russian style with open floor and Pechka, The promotion at work enabled participants to move to multi-story residences, their moving time varied from 1970 to 1991, and the residential moving determined their current housing status. 3. Multi-story residences were available around 1970s, floor-heating system was introduced from 1990s, and high-rise apartments were built from 1998. Korean Chinese(朝鮮族) weren't satisfied with the spatial composition of individual units embedded into the Chinese culture, especially, entrance, kitchen, bathroom and veranda. 4. Based on assimilation through socialism, adaptation to socialist society and capitalist acculturation, the lifestyles of the interviewees were categorized into five types - capitalist-proactive(Ms. KS), socialist-pragmatic(Ms. J), socialist-inducive(Ms. KY), family centered-conservative(Ms. L), and socialist-adaptive(Ms. P). This study implies that housing-related services for Korean Chinese are necessarily provided so as to embrace their life style and cultural identity in housing design, and further studies need to be explored.

• Archives of design research
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• v.19 no.5 s.67
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• pp.281-292
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• 2006

Modern cities' paradigm is rapidly changing to evolve into multi-functional spaces including living space, culture, welfare and environment. Therefore, upon these changing needs, the cultural images and the quality control became major issues in this area. This thesis will examine cultural contents development, a successful case-study, and the analysis of complex space development plan. Thereby each development case in cultural contents area will be extracted and it will seek to find out how they could make a complex cultural space. Moreover, the role and boundary of each Project Management case and Project Manager will be analyzed. The analysis showcases the importance of comprehensive and systematic management system. As a result, along with the recognition of the strong ground for Project Management and Project Manager, comprehensive planand the most effective management system for various complex cultural space development plans would be PM application which is expected to be new alternatives to improve complex space development quality.

• The Research Journal of the Costume Culture
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• v.31 no.2
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• pp.262-276
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• 2023

This study aims to develop a multi-functional cage for dogs as a house to reduce their anxiety when they go out using cages. This study investigates the types and characteristics of cages and cage preference by surveying men and women in their 20s who use them. The cage product reviews are also analyzed. The research results are as follows: First, domestic dog cages are classified into crate, shoulder, cross (sling bag), backpack, carrier, and stroller types. The crate type is easy to clean and can be used as a house, but it is bulky and therefore inconvenient to carry when using public transportation. The shoulder type is a fabric material with good air permeability but has the disadvantage of being easily soiled. It can be used as a house and is light weight, making it convenient when using public transportation. Second, as a result of consumer research, respondents prefer the shoulder-type fabric over the crate-type plastic material. Third, from the shoulder-type product review, the shape stability, companion dogs' psychological safety, the wearability of companions, and management convenience are derived. Fourth, based on the survey results, a multi-functional cage is developed taking into account the companion dog, companion person, and functional factors.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2007.05a
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• pp.181-187
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• 2007

The world is changing drastically by digital information technology and ubiquitous networking. Meanwhile, with the demographic avalanche, South Korea officially became an 'ultra-aging society' in 2026. Simultaneously, this demographic change will also stimulate a cultural climate which facilitates the formation and dissemination of 'slow culture' from the existent urban 'fast culture' which has been weakening the retirement living. One of the fundamental connections with the slow movement appears in the cooking and dining area. With this point of view, this study aims to identify the needs and behaviors of the elderly in current restaurants and a digitally planned multi-purpose one for the future. Small group workshop was employed for this study. In order to elicit detailed information from the elderly group members, scenarios were developed. A group facilitator kept the discussion on track by asking a series of open-ended questions meant to stimulate discussion. As the results, it was revealed that the elderly were interested in digitally planned restaurant which provides one-stop service including entertainment activities, health related contents and socializing with family. Even though this study took restaurant scenarios, the ideas in them also provide holistic perspectives on the direction of marketing and planning strategies on various future environments and services.

• The Research Journal of the Costume Culture
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• v.18 no.3
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• pp.397-407
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• 2010

This study is about the strife patterns in modern fashion and the characteristics inherent in them. The study was aimed at offering the basic materials to develop the creative design that can show various visual expression effects. In terms of method, the study was led by the analysis of documents; domestic and foreign fashion, collection magazines etc were used to analyze the strife patterns in modern fashion. According to the findings, the study used the rearrangement of the strife patterns at the same intervals, the arrangement of different strife patterns within one single item, the hybrid arrangement of strife patterns and different patterns, and the use of strife patterns for a certain part of clothing for the purse of emphasis or decoration. First, the characteristics inherent in such strife patterns showed clear simplicity using the same repetition of simple lines or the two colors of black and white. Second, strife patterns changed silhouettes by causing optical illusion, and showed the rhythm effects, such as swelling, wave or movement by distorting and controlling lines or forms. Third, based on irregular intervals or multi-strife, the concept of unclear disharmony and inconsistency was shown with the beauty of hybridharmony by blending various strife patterns with each other, or strife patterns with different patterns, and thus expressing composite images.

• The Research Journal of the Costume Culture
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• v.17 no.2
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• pp.309-319
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• 2009

This study's goal is to analyze the color characteristics of Blue used in men's fashion for design developing process. First, we researched the previous studies and examined documents about color characteristics of Blue in general as well as coloration in fashion design and men's fashion. We composed color samples by collecting two-color coloration used in men's fashion collection for 5 years from 2004 S/S to 2008 F/W through a specialized fashion information web-sites. We limited the colors from Blue Green(BG) to Purple Blue(PB). Second, we analyzed the characteristics of hue combination and tone combination. A total of 351 pictures were collected and RGB and HV/C value were converted with Munsell Conversion program(ver.8.0.1). Color data has been sorted to 10 hues and 12 PCCS tones. From this, we were able to figure out that similar/same hue coloration was used more than contrary hue coloration and similar/same tone coloration was used more than contrary tone coloration for Blue. We've limited Blue coloration characteristics of men's fashion to two-color coloration for an analysis; the succeeding study will need to examine on the characteristics of multi-coloration and detailed Blue coloration image by various garments.

• Korean Institute of Interior Design Journal
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• v.15 no.3 s.56
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• pp.24-32
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• 2006

Through the increasing of social economies and cultural activities, the Food service industry have been growing up steadily in spite of low economic activity. This is owing to increasing income, participation of women in public affairs and a dual-income family, automobile culture, desire for healthful food, shorten working hours, changing of cultural style. Moreover, the recent importance of Interior finishing materials by means of fire in the Restaurant, legal fractional multiple use business establishment are recognized of publicity. Therefore, This Study aim object at probing into the characteristic of Interior finishing material and then analysing the characteristics of space and material according to the typical distribution. In the Interior planning of Restaurant, firstly it is need to probe the Characteristics of interior finishing material at the basic element. This study is going to apprehend the constitution of space and difference of proportion or a point of sameness in the characteristics of material. Moreover, the recent importance of Interior finishing materials by means of fire in the Restaurant, legal fractional multiple use business establishment are recognized of publicity. Throughout probe and analysing, it can append more efficiency and elasticity in the usage of interior finishing material.

• Korean Institute of Interior Design Journal
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• no.37
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• pp.88-94
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• 2003

In recent years, the direction of the contemporary design paradigm has been shifting from the western to eastern culture; while the western paradigm focuses more on logic and reasons, the eastern paradigm values more on the emotional factors. Today, the interior architecture, in the professional field of design, is gaining its attention from the public due to its rapid development of multi-purpose intelligent buildings which are expected to grow more in coming years. Users are becoming high in demand for the maximum efficiency 8f aesthetical Intoners. While functionality Is the fundamental element In office design, there are increasing request to express uniqueness in the given space such as including the traditional concepts specially when we are living in the era of "globalization". The effect of both WTO policy and the globalization, brought many multinational or foreign companies to expand in Korea. Their request for interior design is to reflect Korean traditions in the office space in order to present the regional characteristics of Korea. Office designs in Korea has been developing rapidly in terms of its advancement in building technology and visual aesthetics. However, the Korean traditional concepts have been expressed very broadly without any boundary or knowledge. Thus, the objective of this study is to analyze the Korean traditions and design elements expressed in various office spaces on case-by-case basis and to provide the typology and the guidelines for the future designs.e designs.

• Journal of Fashion Business
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• v.11 no.4
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• pp.183-203
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• 2007

This study aims at providing useful fundamental information to re-establish the theories of modern fashion by examining the origination and genealogy of street style. The street styles focusing on caucasoid have a variety of genealogies such as western type, beat, teddy boy, hippie, skinhead, punk, neuron-mantic, indie kid, riot grrrl, grunge and techno cyber punk. In the same period, on the contrary, the streets styles focusing on negroid are zootie, hipster, modernist, rude boy, two-tone, rastafarian, funky, B-boy, fly girl, raggamuffine, bhangra, and acid jazz, which are seen as the culture of the large cities formed along Atlantic Ocean and Caribbean sea like England, America and Jamaica. These have root as the main fashion in western society. Ironically, most of the subculture concentrated on the whites were racists. Because of such a reason, the street styles have been formed as resistance culture that was unable to sympathize with their society and characteristics by distinguishing the whites and the colored people. Zootie or hipster that is one of the street fashion styles was formed in the 1940-50s, while the colored people who lived in the west Indies migrated to England or America. As a minimal modernist style called Ivy look in US, in that time, anti-culture formed by teenagers in whitey, teddy boy and mods fashion can be strictly different from the zootie and hipster. The colored people's street styles of the 1960s developed into aggressive and hard forms from the rude boy and two-tone while their resistance toward the whites was stronger. The rastafarian style researched the peak as the colored people's traditional ethnic characteristics or resistance intention for their freedom in the 1970s. In that time, The colored people's street styles of the 1960s developed into aggressive and hard forms from the rude boy and two-tone while their resistance toward the whites was stronger. The rastafarian style researched the peak as the colored people's traditional ethnic characteristics or resistance intention for their freedom in the 1970s. In that time, the street styles of the whites were mostly the skinhead or hippie. Most of them were racists toward the colored people. The punk type on shown on the whites focused on luxury and exaggerative costume. On the contrary, the funky style of the colored people focused on aggressive nihilism and form. With B-boy, fly girl, reggae, rap music, and break dancing in the 1980s, the subculture gradually told on the high fashion as well as the culture between the whites and the colored people. From such aspects, the colored people tried to maintain their unique traditional characteristics. However, their individual values surged by the coming young generation excluded the colored people's characteristic street styles. Focusing on gender, violence and private success among their major concerns, the raga muffin style that represents multi-races and multi-cultures was formed. The jazz style in the 1990s showed cold post-modernistic eclecticism different from that of the 1940s-50s. Simultaneously, the various classes appeared their street styles by emphasizing on each personality. Now that we are living in multi-cultural society, a human race or nationalism concept is getting obscurer. There is no obvious boundary line in the differences between human race and its fashion.