• Journal of Nutrition and Health
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• v.36 no.7
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• pp.661-666
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• 2003

The study was designed to compare the anti-carcinogenic effect of conjugated linoleic acid (CLA) isomers on colon carcinogenesis in 1,2-dimethylhydrazine (DMH)-treated rats by determining the levels of apoptosis, cell proliferation, eicosanoids and 1,2-diacylglycerol (DAG) in colonic mucosa. Sixty male Sprague Dawley rats were randomly divided into 3 groups depending on the types of CLA isomers, i.e. BT group (no CLA contained), CLA-C group (cis-9, trans11 isomer contained), and CLA- T group (trans-10, cis-12 isomer contained). The experimental diet was composed of protein at 20%, carbohydrate at 56.2%, and fat at 14.5% including 0.8% CLA isomers by weight. The experimental diet was fed for 14 weeks with the initiation of intramuscular injection of DMH, which was injected twice a week for 6 weeks to give total dose of l80mg per kg body weight. Two CLA isomers (c9t11 and t10c12) significantly increased the relative percentage of apoptosis but reduced cell proliferation in mucosal cell and also the levels of PGE$_2$, TXB$_2$, and DAG in colonic mucosa. However, there was no significant differences in anti-carcinogenic effect between c9t11 isomer and t10c12 isomer. Overall, colon carcinogenesis could be significantly inhibited by CLA isomers by increasing apoptosis and reducing cell proliferation, the levels of eicosanoids and DAG in colonic mucosa.

• Journal of Oral Medicine and Pain
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• v.20 no.2
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• pp.461-475
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• 1995

The purpose of this study was to investigate the effect of alcohol and stress on liver and buccal mucosa in guinea pig by immunological methods. Especially, Cytochrome P450 (CYP) which in oxidase during alcohol metabolism and bioactivator to carcinogen was used as an indicator in this study. 48 guinea pigs were used in this study. The experimental guinea pig were divided into three groups: The first was a group with giving alcohol-15%(v/v) ethyl alcohol, the second group was a with giving stress in the $0^{\circ}C$ water and the third was a control group. Every 4 guinea pigs of each group were sacrificed weekly-first, second, third, fourth week after experiment and extracted liver tissues and buccal mucosa. The liver tissues were observed by using immunoblotting technique (Western blot) and buccal mucosa were observed by immunofluorescence technique. The results were as follows: 1. By the alcohol and stress, Cytochrome P450 was amplified positive in the liver tissues at third week. 2. By the alcohol and stress, Cytochrome P450 was not detected in the buccal mucosa at any period.

• Journal of Oral Medicine and Pain
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• v.22 no.2
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• pp.261-282
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• 1997

This study was undertaken to compare and investigate the wound healing process following Nd:YAG laser wounds and scalpel incisions of the rat tongue dorsal mucosa. Laser wounds were made parallel on the right side of tongue dorsum and scalpel incisions were made on the left side with 5mm length, respectively Visual inspection and immunohistochemistry with CD44 antibody after immediately, 6 hours, 1 day, 2, 4, 7, 11, 21 days after surgery were carried out. The results were as follows : 1. There was macroscopically no treat difference between laser wounds and scalpel incisions. 2. Laser wounds appeared as dry, bloodless and irregular grooves with carbonization and whitening of superficial tissues compared to sharp, straight and bleeding incisions using scalpel. 3. In the epithelium of rat tongue mucosa, CD44 expression in healing laser wounds and scalpel incisions were similar. 4. In the lamina propria and muscle layer, CD44 expression in healing laser wounds gradually increased earlier and decreased faster than scalpel incisions. Conclusively, it is suggested that pulsed Nd:YAG laser has more beneficial effect on the healing of wounded oral mucosa than scalpel in knew of CD44 expression.

• The Journal of the Korean dental association
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• v.9 no.5
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• pp.235-239
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• 1971

The effects of folic acid on the oral mucosa of albino rats were histochemically studied in the twenty four male rats, weighing abut 100gm. Seven groups of 3 each were injected with 0.2ml folic acid (folic acid 0.5mg. was dissolved in physiological saline 0.2ml) subcutaneously, during 1,2,3,5,7,10 and 14 days respectively. Oral mucosa of rats sere removed from upper molar region and fixed in 10% formalin, cold absolute alcohol, Carnoy's solution and acetone. The serial sections were histochemically stained by McManu's PAS reaction, Mowry's metachromasia, alloxan-Schiff reaction, and azo dye method for alkaline phosphatase. The comparative staining method was hematoxylin-eosin stain. The results were as follows: 1) Alkaline phosphatase reaction of stratum spinosum and stratum granulosum tended to increase after 7 and 10 days of folic acid administration. 2) PAS reactions of basement membrane and lamina propria increased after folic acid administration. 3) Metachromasia of stratum spinosum and stratum granulosum were slightly increased after 3,5 and 7days of folic acid administration and returned to the level of Control after 10 days. 4) In the oral mucosa, alloxan-Schiff reaction increased after 7 days of folic acid administration.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.6 no.2
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• pp.183-186
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• 2003

Prolapse gastropathy is not uncommon in adult, but is not reported yet in previously healthy children. A 3-year-old child came to our emergency room after a 1-day history of emesis episodes with coffee-ground hematemesis. During the endoscopic procedure, and the process of retching and vomiting was observed and a tense knuckle of gastric mucosa was seen to be forcefully and repeated prolapsed into the distal esophageal mucosa, and mucosal hematoma was found in the gastric fundus. Upper gastrointestinal study revealed no abnormality and 24 hour pH monitoring revealed no pathologic gastroesophageal reflux. Retching is thought to cause the forceful prolapse and induce subsequent trauma of gastric mucosa. This case illustrates that the episodes of vigorous retching and resultant gastric mucosa are now considered to be the cause of the hematemesis and epigastric pain in children.

• The Journal of the Korean dental association
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• v.49 no.9
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• pp.520-526
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• 2011

Vascularized iliac crest flap include bone tissue of good quality and quantity for mandible segmental defect. Even if fibular flap can contain longer bone tissue, iliac crest has esthetic shape for mandible body reconstruction and large height for implant. Conventional vascularized iliac crest osteomyocutaneous flap is too bulky for reconstruction of intraoral soft tissue defect. But modified flap can reduce soft tissue volume, so is good for functional reconstruction of oral mucosa. It takes only one month for completely replace oral mucosa. The final mucosal texture is much better than other skin paddle flap, especially for implant prosthesis. Donor site morbidity of this method looks same level or less with other modalities functionally and socially. In case of oral mucosa-mandible combined defect, vascularized iliac crest with internal oblique muscle flap shows good outcomes for hard and soft tissue.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.13
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• pp.5489-5492
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• 2015

Background: Gradual loss of cytokeratin 13 (CK13) may be linked with the severity of dysplastic changes and transformation to malignancy. In this study we assessed the differential expression of CK13 in normal, hyperplastic, dysplastic and cancerous oral mucosa. Materials and Methods: A total of 93 oral biopsies were collected during the 2011-2014 period. The biopsies were characterized as normal (19), hyperplastic (21), severely dysplastic/carcinoma in situ (16) and invasive oral squamous cell carcinoma (OSCC) (37) after morphological assessment. Formalin fixed paraffin embedded sections were stained with a monoclonal antibody against CK13 using the Envision technique. Immunohistochemically stained slides were then analyzed for CK13 expression. Results: CK13 was consistently and diffusely expressed in all normal and hyperplastic tissue biopsies from oral mucosa. Severely dysplastic/carcinoma in situ biopsies showed complete loss in 50% of cases, while in the remaining 50% expression was very focal and weak. OSCC cases showed complete or near complete loss of CK13 in all cases. Few cases showed weak expression in keratin pearls only. Conclusions: This study validates the utility of CK13 IHC as a useful immunohistochemical marker in routine diagnostic practice to make distinction between non-neoplastic from dysplastic and neoplastic (malignant) oral lesions.

• The Journal of Korean Medicine
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• v.24 no.2
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• pp.94-108
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• 2003

Objectives : This study was carried out to investigate the anti-ulcer effects of Pyeongwibunsoeum extract and Misoprostol on indomethacin-induced gastric mucosal lesions of mice. Methods : Experimental groups were classified into non-treated group, non-administered group, Misoprostol-administered group and Pyeongwibunsoeum-extract-administered group. This study examined the morphological change, distribution of mast cells and apoptic cells, PNA reaction, COX-1, BrdU, NF-B p50, COX-2, IL-2R-, and ICAM-1of gastric mucosa. Results : 1. The hemorrhagic erosion of gastric mucosa was reduced in the Misoprostol-administered and Pyeongwibunsoeum-extract-administered groups. 2. The Misoprostol-administered group and Pyeongwibunsoeum-extract-administered group showed positive reaction on the PNA distribution. 3. The distribution of apoptic cells, activity of NF-B p50, COX-2, IL-2R-, and ICAM-1 decreased in both the Misoprostol-administered group and Pyeongwibunsoeum-extract-administered group. 4. The Misoprostol-administered and Pyeongwibunsoeum-extract-administered groups showed increase on COX-1, BrdU. Conclusions : As the results indicate, hemorrhagic erosion of gastric mucosa were reduced in both the Misoprostol-administeredand Pyeongwibunsoeum-extract-administered groups, and severe inflammatory reaction of gastric mucosa was reduced. The effects on the Pyeongwibunsoeum-extract-administered group were superior to those on the Misoprostol-administered group. Pyeongwibunsoeum extract can be widely administered for gastric ulcer disease in clinical treatments.

• Korean Journal of Veterinary Pathology
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• v.5 no.1
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• pp.5-8
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• 2001

Prevalence of Helicobacter infection in pet dogs and positive relations between the presence of Helicobacter app. and gastritis were studied. Twenty-one dogs, which died of various disease, were referred from three animal hospitals and necropsied. Upon histopathological examination, Helicobacter-like organisms (HLO) were observed only in the gastric mucosa of 5 (23.5%) out of the 21 stomachs. The bacteria mainly colonized in the mucus, gastric pits, and the lumen of gastric glands. Regardless of HLO infection, there was mild to moderate lymphocytic infiltration in fundic and pyloric mucosa. Average gastritis scores of the group infected with HLO were 1.250${\pm}$0.214 and 1.833${\pm}$0.167 in the fundic and pyloric mucosa, whereas those of uninfected group were 1.000${\pm}$0.165 and 1.625${\pm}$0.239, respectively. Accordingly, we concluded that no association exists between HLO infection and gastritis.

• The Journal of Korean Society for Radiation Therapy
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• v.7 no.1
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• pp.92-96
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• 1995

All patients who will Undergo irraidiation of the oral cavity cancer will need dental before and during Radiotherapy. The extent of the region and the presence of numerous critical normal tissues(mucosa, gingiva, teeth and the alveolar ridge, alveolar bony structure, etc) in the oral cavity area, injury to which could result in serious functional impairment. Therefore I evaluate the Usefulness of custom-made intraoral shielding device before and during Radiotherapy in oral cavity cancer. Materials and Methods(1) : Manufacture process of Custom-made intraoral shielding device Containing Cerroband. A. Acquisition of impression B. Matrix Constitution C. Separation by Separator D. Sprincle on method E. Trimming F. Spacing G. Fill with Cerroband Materials and Methods (2) A. Preannealing B. TLD Set up C. Annealing D. TLD Reading = Results = Therefore dosimetric characteristics in oral cavity by TLD Compared to isodose curve dose distribution Ipsilateral oral mucosa, Contralateral oral mucosa, alveolar ridge, tongue, dose was reduced by intraoral shielding device containning Cerroband technique Compard to isodose plan = Conclusions = The custom-made intra-oral shielding device containing Cerroband was useful in reducing the Contralateral oral mucosa dose and Volume irradiated.