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Effect of Dietary CLA Isomers on Apoptosis and Cell Proliferation in Colonic Mucosa of DMH-Treated Rats  

박현서 (경희대학교 생활과학대학 식품영양학과)
권필수 (경희대학교 생활과학대학 식품영양학과)
윤정한 (한림대학교 생명과학부)
하영래 (경상대학교 응용생명과학부)
Publication Information
Journal of Nutrition and Health / v.36, no.7, 2003 , pp. 661-666 More about this Journal
Abstract
The study was designed to compare the anti-carcinogenic effect of conjugated linoleic acid (CLA) isomers on colon carcinogenesis in 1,2-dimethylhydrazine (DMH)-treated rats by determining the levels of apoptosis, cell proliferation, eicosanoids and 1,2-diacylglycerol (DAG) in colonic mucosa. Sixty male Sprague Dawley rats were randomly divided into 3 groups depending on the types of CLA isomers, i.e. BT group (no CLA contained), CLA-C group (cis-9, trans11 isomer contained), and CLA- T group (trans-10, cis-12 isomer contained). The experimental diet was composed of protein at 20%, carbohydrate at 56.2%, and fat at 14.5% including 0.8% CLA isomers by weight. The experimental diet was fed for 14 weeks with the initiation of intramuscular injection of DMH, which was injected twice a week for 6 weeks to give total dose of l80mg per kg body weight. Two CLA isomers (c9t11 and t10c12) significantly increased the relative percentage of apoptosis but reduced cell proliferation in mucosal cell and also the levels of PGE$_2$, TXB$_2$, and DAG in colonic mucosa. However, there was no significant differences in anti-carcinogenic effect between c9t11 isomer and t10c12 isomer. Overall, colon carcinogenesis could be significantly inhibited by CLA isomers by increasing apoptosis and reducing cell proliferation, the levels of eicosanoids and DAG in colonic mucosa.
Keywords
CLA isomers; apoptosis; cell proliferation; eicosanoid; DAG; colon cancer;
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1 Identification of programmed cell death via specific labeling of nuclear DNA fragmentation /
[ Gavrieli,Y;Sherman,Y;Ben-Sasson,S.A. ] / J Cell Biol   DOI   ScienceOn
2 Conjugated linoleic acid and disease prevention:a review of current knowledge /
[ MacDonald,H.B. ] / J Am Coll Nutr   DOI
3 An increase in phosophatidic acid in the absence of changes in diacylglycerol in human platelets stimulated with ADP /
[ Duncan,E.M.;Lyoyd,J.V. ] / Int J Biochem   DOI   ScienceOn
4 A programmed cell death and its protective role with particular reference to apoptosis /
[ Schulte-Hermann,R.;Bursch,W.;Kraupp-Grasl,B.;Oberhammer,F.;Wagner,A. ] / Toxicology Letters   DOI
5 Antiplatelet effects of conjugated linoleic acid isomers /
[ Truitt,A.;McNeill,G.;Vanderhoek,J.Y. ] / Biochim et Biophy Acta   DOI   ScienceOn
6 Induction of apoptosis by conjugated linoleic acid in cultured mammary tumor cells and premalignant lesions of the rat mammary gland /
[ Ip,C.;Ip,M.M.;Loftus,T.;Shoemaker,S.;Shea-Eaton,W. ] / Cancer Epidemiol Biomarkers Prev
7 /
[ Granstron,E.;Samuelson,B.;J.C.Frolich(ed.) ] / Advances in prostaglandin and thromboxane research
8 Conjugated linolic acid inhibits proliferation and induces apoptosis of normal rat mammary epithelial cells in primary culture /
[ Ip,M.M.;Masso-Welch,P.A.;Shoemaker,S.F.;Shea-Eaton,W.K.;Ip,C. ] / Exp Cell Res   DOI   ScienceOn
9 CLA reduces antigen-induced histamine and PGE2 release from sensitized guinea pig tracheae /
[ Whigham,L.D.;Cook,E.B.;Stahl,J.L.;Saban,R.;Bjorling,D.E.;Paraza,M.W.;Cook,M.E. ] / Am J Physiol Regulatory Integrative Comp Physiol
10 Dietary fat, fiber and carcinogen alter fecal diacylglycerol composition and mass /
[ Pickering,J.S.;Lupton,J.R.;Chapkin,R.S. ] / Cancer Res
11 Immunohistochemical detection of abnormal cell proliferation in colonic mucosa of sub-jects with polyps /
[ Wilson,R.G.;Smith,A.N.;Bird,C.C. ] / J Clin Pathol   DOI
12 Studies with anti-bromo deoxyuridine antibodies:II. Simultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa /
[ Schutte,B.;Reyders,M.M.J.;Bosman,F.T.;Blijham,G.H. ] / J Histochem Cytochem   DOI   ScienceOn
13 Effect of anthraquinone-laxatives on the proliferation and urokinase secretion of normal, premalignant and malignant colonic epithelial cells /
[ Schorkhuber,M.;Richter,M.;Dutter,A.;Sontag,G.;Marian,B. ] / Eur J Cancer   DOI   ScienceOn
14 Dietary conjugated linoleic acid (CLA) induces apoptosis of colonic mucosa in 1,2-dimethylhydrazine-treated rats:a possible mechanism of the anticarcinogenic effect by CLA /
[ Park,H.S.;Ryu,J.H.;Ha,Y.L.;Park,H.Y. ] / Brit J Nutr   DOI   ScienceOn
15 Possible mechanism for the putative antiatherogenic and antitumorigenic effects of conjugated polyenoic fatty acid /
[ Farquharson,A.;Wu,H.-C.L.;Grant,I.;Graf,B.;Choung,J.-J.;Eremin,O.;Heys,S.;Wahle,K. ] / Lipid   DOI
16 Inhibition of apoptosis during development of colorectal cancer /
[ Bedi,A.;Pasricha,P.J.;Akhtar,A.J.;Barber,J.P.;Bedi,G.C.;Giar-diello,F.M.;Zehnbauer,B.A.;Hamilton,S.R.;Jones,R.J. ] / Cancer Res
17 Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes /
[ Liu,K.L.;Belury,M.A. ] / Cancer Letters   DOI   ScienceOn
18 Inhibition of carcinogenesis by conjugated linoleic acid:potential mechanisms of action /
[ Belury,M.A. ] / J Nutr
19 The biologically active isomers of conjugated linoleic acid /
[ Pariza,M.W.;Park,Y.H.;Cook,M.E. ] / Progress in Lipid Research   DOI   ScienceOn
20 A rapid method of total lipid extraction and purification /
[ Bligh,E.G.;Dyer,W.J. ] / Can J Biochem Physiol
21 A colorimetric method for estimating serum triglycerides /
[ Fletcher,M.J. ] / Clin Chim Acta   DOI   ScienceOn
22 Effect of dietary supplementation of conjugated linoleic acid on tumor incidence and colon carcinogenesis in I,2-dimethylhydrazine-treated rats /
[ Kim,K.H. ] / Doctoral Dissertation