• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.33-33
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• 2003

Lipoprotein lipase (LPL)은 지방축적과 대사에 있어 중요한 효소로서 지방조직과 근육 내로의 지방산 유입을 조절하며, 여러 조직에서 합성되고, 조직 특이적인 방법으로 동물의 생리상태, 영양상태 및 발달단계에 따라 유전자 발현이 조절되는 것으로 알려져 있다. 본 연구는 어류의 체지방 축적과 대사과정을 이해하고 성장 및 경제형질 관련 DNA marker를 확보하기 위한 1차적인 연구로서, 한국산 선발계통 및 일본산 양식계통과 이들 두 계통간 잡종 참돔 집단을 이용하여 LPL 유전자 내의 다형성을 탐색하고 분석하였다. PCR-RFLP 분석을 실시하여 참돔 LPL 유전자 exon 2번을 포함하는 영역에서 3개의 (Msp I, Alu I 및 Hsp92II) 다형성을 확인하였고, 각 집단간 대립유전자의 빈도를 분석하였다. Exon 2번에서 관찰된 Msp I 다형성은 염기치환 (C$\longrightarrow$G)이 일어난 형태로서 아미노산 서열에는 변화가 없는 silent mutation 이었으며, 대립유전자의 빈도를 분석한 결과, 각 집단간 뚜렷한 차이는 없었다. Alu I 및 Hsp92II 다형성은 intron 영역에서 발견되었으며, Alu I 다형성으로 인한 4개의 대립유전자형 중 D 대립유전자는 한국산 선발 계통과 한국산 선발계통을 포함하는 교배집단에서만 검출되었다. 이 후의 연구에서는 참돔 LPL 유전자의 exon 영역에 존재하는 다형성을 탐색하고, 형질과의 연관성 및 지방축적 기능과의 관련성 등을 분석하고자 한다.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.369-374
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• 2008

The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.13-22
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• 2003

Growth Hormone (GH) gene is a member of gene family through the evolutionary process from a small common ancestral gene by a series of gene duplications. The role of the GH in growth and performance controls has been extensively studied in human, mice and livestock. Many researchers have considered GH as a strong candidate gene for evaluation of genetic polymorphisms that could be associated with economic traits in cattle. We report here a novel missense mutation within the exon 5 of the bovine Growth Hormone (bGH) gene. We could amplified 522 bp fragments from eight unrelated Hanwoo cattle by PCR, then, subsequently cloned and sequenced. An Msp I RFLP corresponding to a C to T transition was observed at position 2258 nt. From this result, we could predict a missense mutation (Arg to Trp) at codon 166 in a highly conserved region among many mammals. Codominant Mendelian segregation of the two alleles, Msp I (+) and Msp I (－), was observed in two full-sib F2 families (n = 32, African taurine Bos taurus ${\times}$ African zebu Bos indicus) and eight half-sib Hanwoo families. For the availability of genetic marker, we have performed PCR-RFLP with a large number of individual animals from 15 different cattle breeds (European and Asian taurines, and African indicines). Consideration of breed frequencies of Msp I (－) allele in relation to breed type and their geographic origins, shows higher frequencies in humped breeds or Asian cattle breeds than in humpless or European breeds. This result indicates that the missense mutation can be contributed the functional significance such as the signal transduction through the receptor binding, also may be used as a marker for selection of the economic traits in Hanwoo.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.455-460
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• 2010

A polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis was applied to detect and identify ten Weissella spp. frequently found in kimchi. The previously reported genus-specific primers designed from 16S rDNA sequences of Weissella spp. were adopted but PCR was performed at the increased annealing temperature by $4^{\circ}C$. The sizes of amplified PCR products and restricted fragments produced by AluI, MseI, and BceAI endonucleases were well correspond with the expected sizes. W. kandleri, W. koreensis, W. confusa, W. minor, W. viridescens, W. cibaria, and W. soli were distinguished by AluI and MseI and W. hellenica and W. paramesenteroides were identified by BceAI. W. thailandensis was distinguished when restriction pattern of other species was compared but identified by the single use of MspI.

• Parasites, Hosts and Diseases
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• v.35 no.4
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• pp.271-276
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• 1997

We tried to compare the three kinds oi Metagonimur species. M. Wokognulci, Ifetafonimus Miyata type, and M. tnknhashii, which were Know to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR- RFLP) patterns. We amplified the internal transcribed spacer 1 (ITSI) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITSI gene loth Rsc I, ALu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metcgonimus. In case of mc01 gene, Rsc I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M Wokognwai and Metasoninus Miyata type was 0.034880, between Metusoninus Miyata type and M. tckc- hushii was 0.028098, between M. wokogawai and M. tnkahashii was 0.018179. It is suggested that Metasonimus Miyata type may be separate species and evolutionize at the older time than the other two species.

• Environmental Mutagens and Carcinogens
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• v.22 no.1
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• pp.39-46
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• 2002

Obesity is a complex metabolic disorder with a strong genetic component. There are many candidate genes for obesity and its related phenotypes. We studied genetic variations between Korean obese and lean groups. Polymorphisms investigated were the Msp I polymorphism of the $\alpha$$_{2A}$-adrenergic receptor ($\alpha$$_{2A}$-AR) gene, the Mnl I polymorphism of the $\alpha$$_2$-adrenergic receptor ($\alpha$$_2$-AR) gene, the BstO I polymorphism of the $\beta$$_3$-adrenergic receptor ($\beta$$_3$-AR) gene, the Pml I polymorphism of the lamin A/C (LMNA) gene, the Hga I polymorphism of the clearance receptor (NPRC) gene, the Msp I polymorphism of the leptin gene, BclI polymorphism of the uncoupling protein 1 (UCPI) gene and the Hha I polymorphism of the fatty acid binding protein 2 (FABP2) gene. Among these genetic markers, Pml I polymorphism at the LMNA gene and Bcl I polymorphism at the UCP1 gene were significantly associated with obesity. However, further studies are required whether thease findings are reproduced in large population, although two polymorphisms might be useful as genetic markers in the ethiology of obesity in Korean population.ion.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.2
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• pp.141-147
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• 2004

Objective: To investigate the association between endometriosis and polymorphisms of N-acetyl transferase 2 (NAT2), glutathione S-transferase M1 (GSTM1), and cytochrome P450 (CYP) 1A1 genes in Korean infertile patients. Materials and Methods: A total of 303 infertile patients who had undertaken diagnostic laparoscopy during January, 2001 through December, 2003 at Samsung Cheil Hospital enrolled in this study. The patients were grouped according to laparoscopic findings: minimal to mild endometriosis (group I: n=147), moderate to severe endometriosis (group II: n=57), normal pelvic cavity (n=99). Peripheral blood was obtained and genomic DNA was extracted. The genotypes of each genes were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). For NAT2, RFLP was used to detect the wild type (wt) and mutant (mt) alleles, enabling classification into slow (mt/mt) or fast (wt/wt or wt/mt) acetylation genotypes. For GSTM1, PCR was used to distinguish active (+/- or +/+) from null (-/-) genotypes. For CYP1A1, MspI digestion was used to detect the wild type (A1A1), heterozygote (A1A2) or mutant (A2A2) genotypes. Result: The genotype frequencies of NAT2 slow acetylator was 12.8%, 10.9%, 12.8% in group I, group II and control, respectively. The genotype frequencies of GSTM1 null mutation was 55.3%, 41.8%, 53.2% in group I, group II and control, respectively. The genotype frequencies of CYP1A1 MspI polymorphism was 16.3%, 9.1%, 18.1% in group I, group II and control, respectively. No significant difference was observed between endometriosis and normal controls in the genotype frequencies of the NAT2, GSTM1, CYP1A1 MspI polymorphism. Conclusion: The NAT2, GSTM1, CYP1A1 gene polymorphism may not be associated with the susceptibility of endometriosis in Korean women.

• Proceedings of the KIPE Conference
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• 2006.06a
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• pp.155-157
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• 2006

Electrical motors are an integral part of industrial plants with no less than 5 billion motors built word wide every year and the BLDC motor have been increased demand on industrial application. This paper presents BLDC motor control algorithm for cost effective motor application using general purpose microprocessor which have only one on-chip timer and describes how to realize pulse width modulation(PWM) signals with general input/output(I/O) ports to control a three-phase permanent magnet brushless do motor using timer interrupt on MSP430F1232.

• Journal of Power Electronics
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• v.7 no.2
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• pp.132-139
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• 2007

Electrical motors are an integral part of industrial plants with no less than 5 billion motors built world wide every year. The demand for low-cost brushless DC (BLDC) motors has increased in industrial applications. This paper presents a BLDC motor control algorithm for low-cost motor drive applications using general purpose microcontrollers which have only one on-chip timer. This paper describes how to realize pulse width modulation (PWM) signals with general input/output (I/O) ports to control a three-phase permanent magnet brushless DC motor using the timer interrupt on MSP430F1232.

• The Korean Journal of Mycology
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• v.33 no.2
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• pp.98-102
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• 2005

In 2003 to 2005, the root rot of Japanese angelica (Aralia elata) was surveyed in nursery beds of Korea, where incidence of the disease often reached up to 100%. Three isolates were obtained from the infected roots, and identified as Phytophthora cactorum on the basis of cultural, morphological characteristics and molecular analysis. The isolates were characterized by having markedly papillate and broadly ovoid deciduous sporangia. The optimum temperature for mycelium growth was at $25^{\circ}C$ on V8 juice agar. Pathogenicity of the isolates was confirmed by soil mixture inoculation. Approximately 900 bp of ITS rDNA was amplified from all 3 isolates and band pattern of restriction fragments observed by Alu I, Msp I, and Taq I digestion also supported the result of the morphological identification when compared with PhytID database.