• 한국의사학회지
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• 제35권1호
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• pp.37-44
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• 2022

Uirimchalyo (醫林撮要) was published in advance of the Donguibogam (東醫寶鑑) in the middle of the Joseon Dynasty. This book was a pioneering form of the Donguibogam. This paper examines the acupuncture and moxibustion methods used within Uirimchalyo. Because this book was produced for the general public as well as professional Korean medicine doctors, it did not present complex symptom classification and complicated acupuncture prescriptions. Instead, it summarized acupoints in a simple way and could be used as a standardized treatment guideline. In addition, in the acu-moxa methods of the Uirimchalyo, moxibustion was used more than acupuncture. This implies that the author's intention was to treat the disease gently, and furthermore, that the book inherits the acu-moxa method of Hyangyakjipseongbang (鄕藥集成方). There are many suggestions of experienced prescriptions, which can be seen as focusing on experience-based medicine. In addition, the characteristic of not using many acupuncture points served as the cause of the emergence of Saam acupuncture techniques.

• Journal of Acupuncture Research
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• 제22권1호
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• pp.19-27
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• 2005

${\ll}$상한론(傷寒論)${\gg}$ 태양병(太陽病) 제강(提綱)의 침구학적(鍼灸學的) 분경(分經) 및 분증(分證)의 운용(運用) 방법(方法)을 연구(硏究)한 결과(結果)는 다음과 같다. 1. ${\ll}$상한론(傷寒論)${\gg}$의 육경변증(六經辨證)은 ${\ll}$소문(素問) 열론(熱論)${\gg}$의 육경분증(六經分證)을 기본(基本)으로 하여 계승(繼承)하여 발전(發展) 되었다. 2. 태양병(太陽病) 제강(提綱)은 풍한(風寒), 온열(溫熱), 역병(疫病) 및 잡병(雜病)으로 인해(因) 태양경(太陽經)으로 이환(罹患)되는 증상(症狀)의 분경(分經) 정증(定證)의 강영(綱領)이 된다. 3. ${\ll}$상한론(傷寒論)${\gg}$ 주석가(注釋家)들은 태양병(太陽病)의 제강(提綱)을 인체(人體)의 피부(皮膚)를 주관(主管)하는 족태양승광경(足太陽勝胱經)의 영,(榮) 위기(衛氣)의 생리(生理) 병리적(病理的) 방면(方面)으로 해석(解釋)하였다. 4. 태양병(太陽病)의 진구(鎭灸) 치료(治療)의 혈위(穴位)는 수 (手) 족태양경(足太陽經)과 독맥(督脈)의 요혈(要穴)을 중심(中心)으로 '관기맥증(觀其脈證) 지범하역(知犯何逆) 수증취혈(隨證取穴)' 하는 변증논치(辨證論治)의 관점(觀點)으로 용침(用鍼)한다.

• Journal of Acupuncture Research
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• 제29권1호
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• pp.15-24
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• 2012

Objectives : In recent years, many studies have been widely researching anti-inflammation effect of various medicinal plants. $Cinnamomi$ $Cortex$ was not enough in researching of the anti-inflammation. Moreover, there is no comparative study about extraction methods. Therefore, we investigated the inhibitory effects of $Cinnamomi$ $Cortex$ pharmacopuncture by EtOH and Hot water extraction on Nitric oxide(NO), Prostaglandin E2(PGE2) production, Cyclooxygenase(COX)-2, inducible NOS(iNOS) expression and extracellular signal regulate kinase(ERK)1/2 phosphorylation in lipopolysaccharide(LPS) induced RAW 264.7 macrophage cell. Methods : $Cinnamomi$ $Cortex$ was extracted by EtOH and Hot water. RAW 264.7 macrophage cell viability was measured by MTT assay. Effect of $Cinnamomi$ $Cortex$ pharmacopuncture on NO and PGE2 production in LPS induced macrophages was accessed by Griess assay and enzyme-linked immunospecific assay(ELISA), respectively. Inhibition effect on COX-2, iNOS expression and ERK1/2 phosphorylation was examined by Immunoblotting assay. Results : 1. Cytotoxic effect of $Cinnamomi$ $Cortex$ pharmacopuncture by Hot water extraction in RAW 264.7 macrophages was not appeared, except $3125{\mu}g/m{\ell}$. And cytotoxic effect was not appeared in EtOH extraction method. 2. $Cinnamomi$ $Cortex$ pharmacopuncture by EtOH and Hot water extraction inhibited NO production in LPS induced macrophages significantly. 3. $Cinnamomi$ $Cortex$ pharmacopuncture by EtOH and Hot water extraction inhibited PGE2 production in LPS induced macrophages significantly. 4. $Cinnamomi$ $Cortex$ pharmacopuncture by EtOH and Hot water extraction inhibited COX-2, iNOS expression in LPS induced macrophages. Especially, it has been confirmed that COX-2, iNOS expression were effectively inhibited in Hot water extraction. 5. $Cinnamomi$ $Cortex$ pharmacopuncture by EtOH and Hot water extraction inhibited ERK1/2 phosphorylation in LPS induced macrophages. Especially, it has been confirmed that ERK1/2 phosphorylation was effectively inhibited in Hot water extraction. Conclusions : According to the results, $Cinnamomi$ $Cortex$ pharmacopuncture suppresses NO, PGE2 production, COX-2, iNOS expression and ERK1/2 phosphorylation in LPS induced macrophages. It has a potential for treating various inflammatory diseases, and Hot water extraction method could be used more extensively than EtOH extraction method.

• 대한약침학회지
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• 제12권4호
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• pp.33-50
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• 2009

Objectives : This study was conducted to confirm validation and stability of concentration analysis method of pure melittin (Sweet Bee Venom-Sweet BV) extracted from the bee venom by utilizing protein isolation method of gel filtration. Methods : All experiments were conducted at Biotoxtech, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Standard solutions of melittin (SIGMA, USA) and test substances were dispensed and were analyzed with HPLC for Sweet BV to secure the validation of analysis. Results : 1. Measurement of system suitability of Sweet BV satisfied criterion of below 3%. 2. Confirming Linearity of Sweet BV in 10-200${\mu}g/m\ell$ solution yielded correlation coefficient (r) of 0.995 and accuracy of 85-115% which satisfy criterion. 3. Measurement of Specificity of Sweet BV didn't yield any substance affecting the peak of test substances, but detected at 21.22min verified as the test substance. 4. Confirming Intra-day of Sweet BV, accuracy and precision of 0.1, 100${\mu}g/m\ell$ were 105.70, 95.81 and 0.66, 0.73, respectively, satisfying both criteria of accuracy (85-115%) and precision (within 10%). 5. To measure Stability in autosampler, all samples used in Intra-day reproducibility sat in the autosampler for five hours and were re-analyzed. Both variability and precision satisfied the criteria. 6. Homogeneity of Sweet BV (0.1, 100${\mu}g/m\ell$) at upper, middle, and lower layers all satisfied the accuracy and precision criteria. 7. Stability of Sweet BV (0.1, 100${\mu}g/m\ell$) at room temperature for four hours and refrigerated for 7 days all satisfied the criterion. 8. For the measurement of Quality control, QC samples measured on the first and eighth day all satisfied accuracy and precision criteria. Conclusion : Above experiment data satisfies validation and stability of concentration analysis method of Sweet BV.

• 대한한의학원전학회지
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• 제31권3호
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• pp.59-75
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• 2018

Objectives : Contents regarding Qiuzimen as found in Beijiqianjinyaofang and Qianjinyifang were studied for their potentialities to be applied to infertility clinics. Methods : The contents on Qiuzimen in Beijiqianjinyaofang and Qianjinyifang are pondered upon after dividing into two categories of medical theory and therapeutic principle, and other related texts and dissertations are further studied to discover a potential subject matter in being applied to today's modern clinics. Results : 1. In medical theory, Sunsimiao picked five overexertions and seven damages as the main medical cause of infertility. For remedy, he established four common formula, which are: chinjasan, bakchotangpotang, suppository, and haseog(cheon)moondongwhan. 2. There are total of thirteen types of formula described in the Qiuzimen in Beijiqianjinyaofang and Qianjinyifang, and five acupuncture points are used in the moxibustion method. After analyzing the herbs that were used six or more times, it was discovered that medicines were used to tonify the viscera of heart and kidney that are damaged through five overexertions and seven damages. In moxibustion method, different points such as CV4, LR14, pomoon, cheonmoon, and KI2, which is the point for kidney meridian were used in order to tonify the uterus through lower abdomen area and reproductive organs. 3. Qiuzimen has not only had a great impact on the infertility treatment as found in Furendaquanliangfang, which is the first gynecology text in Song dynasty, but it also had a continuous impact on medical texts in Ming and Qing dynasty. 4. The infertility therapeutic principle and approach method as found in the Qiuzimen are still relevant in today's modern infetility treatment. Conclusions : In the Qiuzimen of Beijiqianjinyaofang and Qianjinyifang, the problem of infertility is viewed as the whole body's problem, namely a type of defective disease caused by five overexertions and seven damages, the view of which should be applied to today's modern clinics.

• Journal of Acupuncture Research
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• 제35권4호
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• pp.182-186
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• 2018

Background: The purpose of this study was to investigate the effects of wild ginseng pharmacopuncture on melanin production in B16/F10 murine melanoma cells. Methods: To determine the effect of wild ginseng pharmacopuncture solution on B16/F10 cells, cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide (MTT) method. To observe B16/F10 cell growth, death, and morphological changes, Trypan blue solution was used. The Hosoi method was used to investigate the effect of wild ginseng pharmacopuncture solution on melanin production. The Martinez-Esparza method was used to investigate the effect of wild ginseng pharmacopuncture solution on tyrosinase activity. To determine the pathway involved in the melanogenesis in cells exposed to wild ginseng pharmacopuncture solution, a cell-free tyrosinase was used. Results: Following treatment with $200{\mu}L$ of wild ginseng solution, the cell survival rate was $76.32{\pm}2.45%$ which significantly decreased with higher concentrations (${\mu}L$) of wild ginseng (up to $200{\mu}L$). When $100{\mu}L$ of wild ginseng was used, the cell survival rate was $89.95{\pm}2.07%$. No morphological changes or abnormalities were observed in the B16/F10 murine melanoma cells as observed in the Trypan blue test. Melanin production was significantly reduced to $72.17{\pm}3.74%$ at $100{\mu}L$. Using $100{\mu}L$ of wild ginseng solution, tyrosinase activity was significantly decreased to $80.15{\pm}1.05%$. Wild ginseng pharmacopuncture solution reduced melanin production both directly and indirectly. Conclusion: This study suggests that wild ginseng pharmacopuncture solution may be effective in inhibiting melanin production. Further studies are needed to determine safe and effective clinical applications.

• 대한약침학회지
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• 제3권2호
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• pp.153-168
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• 2000

This study was designed to study on major components of various Bee Venom(Bee Venom by electrical stimulation in Korea; K-BV I, Bee Venom by Microwave stimulation in Korea; K -BV II, 0.5rng/ml, Fu Yu Pharmaceutical Factory, China; C-BV, 1mg /ml, Monmouth Pain Institute, Inc., U.S.A.; A-BV) using Electrophoresis. The results were summarized as follows: 1. In 1:4000 Bee Venom solution rate, the band was not displayed distinctly usmg Electrophoresis. But in 1: 1000, the band showed clearly. 2. The results of Electrophoresis at solution rate 1:1000, K-BV I and K-BVII showed similar band. 3. The molecular weight of Phospholipase $A_2$ was known as 19,000 but its band was seen at 17,000 in Electrophoresis. 4. Protein concentration of Bee Venom by Lowry method was different at solution rate 1:4000 ; C-BV was $250{wmu}g/ml,\;K-BV\;I\;was\;190{wmu}g/ml,\;K-BVII\;was\;160{wmu}/ml\;and\;C-BV\;was\;45{wmu}/ml5$. Electrophoresis method was unuseful for analysis of Bee Venom when solution rate is above 1:4000 but Protein concentration of Bee Venom by Lowry method was possible. These data from the study can be applied to establish the standard measurement of Bee Venom and prevent pure bee venom from mixing of another components. I think it is desirable to study more about safety of Bee Venom as time goes by.

• 대한본초학회지
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• 제31권2호
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• pp.1-5
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• 2016

Objectives : Seborrheic dermatitis is an inflammatory skin disorder affecting the scalp, face, and torso. Typically, it presents with scaly, flaky, itchy, and red skin. It may be aggravated by illness, psychological stress, fatigue, sleep deprivation, change of season. The purpose of this study was to report the clinical effects of herbal medicine on Seborrheic dermatitisMethods : We employed oriental medical treatments; herbal-medication (Bangpungtongsungsangamibang), acupuncture, moxibustion and cupping therapy. At the same time, the patient sweated out the skin poison. We treated him two times a week with oriental therapy method. He took medicine three times a day after a meal. During taking medicine, we let him avoid fatty food, flour based food, and alcohol. We took a picture the patient's body parts and compared the symptom with previous symptom. With the picture, we evaluated the patient's condition.Results : After taking treatment - 77 times' acupuncture, moxibustion and cupping therapy during 9 months and taking herbal-medicine, the skin condition of the patient was much improved. At first, he was troubled with itching and hot feeling at reddish area, time after time the itching and hot feeling was weakened. The reddish area was changed to scaly and flaky skin and changed to normal state.Conclusions : Herbal medicine (Bangpungtongsungsangamibang) with oriental medical treatments was an effective in the treatment of Seborrheic Dermatitis and it helped to improve regenerating the skin in the body.

• Journal of Acupuncture Research
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• 제28권6호
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• pp.43-51
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• 2011

Objectives : The purpose of this research was to investigate the effect of oriental medicine public-health program on frozen shoulder patients. Methods : Oriental medicine public-health program was done to 50 frozen shoulder patients who visited Eumseong-gun public health center. Oriental medicine treatment(twice a week for 12 weeks) and prevention-educaiton program was included in public-health program. The schedule was proceeded from 16th March 2010 to 3rd June 2010. The efficacy of program was measured by visual analog scale (VAS), ROM(range of motion), Apley scratch test and Life function score, sleep quality score of their first and last visit. Then we analyzed the improvement in the same group. Results : 1. In VAS change, program showed statistically significant improvement. 2. In ROM(flexion, extension, abduction, adduction) and apley scratch test, program showed statistically significant improvement. 3. In Life function score, program showed statistically significant improvement. 4. In sleep quality score, program also showed improvement, but didn't do statistical significans. Conclusions : The above results suggest that oriental medicine public-health program can be used as effective method for frozen shoulder's treatment and care.

• 한방안이비인후피부과학회지
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• 제18권2호
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• pp.62-69
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• 2005

Objective: This study is to explore the various aspects of the fatigue perceived by patients with Bell's palsy Method: 33 patients who were diagnosed as Bell's palsy were sequentially interviewed and examined Results: The results were as follows 1. The mean average fatigue score of subjects was $1.79{\pm}0.41$ : the physical fatigue revealed the highest value with a mean of $1.84{\pm}0.50$, followed by neurosensory fatigue with a mean of $1.77{\pm}0.42$, psychological fatigue with a mean score of $1.76{\pm}0.54$. 2. With the respect to the general characteristics of subjects, there were statistically significant differences in the patients' fatigue perception by age and the amount of sleeping time. Conclusions: We needed the further study about the relation between fatigue and Bell's palsy