• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.60-60
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• 2002

본 연구는 사람의 조혈촉진유전자가 형질전환된 돼지의 유즙내 hEPO 발현농도를 확인하고 3종의 동물 (mouse, rat, swine)에서 hEPO 의 생리활성을 분석하기 위하여 실시하였다. 새롬이 Fl 암컷의 유즙을 원심분리하여 지방을 제거한 후 hEPO 의 농도를 측정하고, PBS 로 유즙내 hEPO 농도가 100 IU/㎖가 되도록 희석하였다. Mouse (ICR)는 피하주사로 20 IU씩 2일 간격으로 3회 (0, 2, 4 일차 ) 주사하였으며, 주사 후 4회에 걸쳐 (0, 2, 4, 7 일차) 혈액을 채취하여. 분석하였다. (중략)

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.732-737
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• 2003

Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [$I^125$ ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.

• Journal of Dairy Science and Biotechnology
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• 제29권1호
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• pp.1-15
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• 2011

국내는 물론 해외에서도 kefir 섭취 요법에 의한 당뇨병의 치료 효과에 관한 연구는 거의 없는 현실이다. 따라서, kefir가 당뇨병 치료 보조식품으로서 이용 가능성이 있는지를 검토하고 연구할 가치가 있다고 본다.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2004년도 제4회 발생공학 국제심포지움 및 학술대회
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• pp.45-45
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• 2004

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.261-261
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• 1994

Bovine milk xanthine oxidase (E.C.1.1.3.22, XO) purchased from Sigma Chemical Co. had the three protein fragments below 150 kDa on 7.5％ SDS-PAGE, which did not show enzyme activity. To remove these fragments, the enzyme preparation was further purified through Sephadex G-200 column chromatography. Two peaks exhibiting enzymatic activity were separated very closely to the void volume, which were revealed as two different enzyme forms, dimeric and monomeric, confirmed by activity staining on native PAGE. Anti sera-against each of the two enzyme forms were raised by subcutaneous injection at multiple sites on the back of rabbits during 4 weeks. On the immunodiffusion test, it was found that both of the antisera of the two forms could react with each other, which implied that their epitopes were identical In the Western blot analysis of mouse liver cytosol fraction, it was found that rabbit anti-XO antibody bound well with the protein band of monomeric mouse liver XO of about 150kDa. Based on this result, mouse liver cDNA 1 ibrary was screened by in situ hybridizat ion wi th rabbi t anti -XO antibody as probe. Through the immunological screening, recombinant phages giving positive signal by the production of XO were selected and further purified. To validate these clones, purified phages were lysogenized in E. coli Y1089 and their lysates were analysed for enzyme activity and immunoreactivity, It was verified that lysates of the purified recombinant phage lysogens exhibited the enzymatic activity as well as bound wi th XO antibody, when induced by IPTG. The above results assert that selected recombinant phage carries mouse liver XO gene.

• 한국미생물·생명공학회지
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• 제24권1호
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• pp.37-43
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• 1996

Augmentation of antitumor activity of antitumor drugs in combination with Lactobacillus casei HY2782 (LC2782) was studied against Sarcoma-180 (S-180) and Lewis lung carcinoma (3LL). Antitumor drugs used in this study were 5-fluorouracil (5-fu) and cyclophosphamide (CP). The prolongation effect of LC2872 on the life span of mouse intraperitoneally implanted with S-180 was stronger than that of OK-432 and BCG, while the inhibitory effect of OK-432 and BCG on the growth of 3LL solid tumor was a little stronger than that of LC2782. Average survival rates of mice administrated LC2782, OK-432 and BCG were 192%, 141%, and 112%, respectively, when that of the control was 100%, Intralesional administration of 5-Fu, CP, 5-Fu+LC2782 and CP+LC2782 resulted in 93%, 69%, 99% and 73% inhibition rates against 3LL solid tumor proliferation. The combination therapy of 5-Fu or CP with LC2782 significantly prolonged the life span of S-180-inoculated ICR mice. Average survival rates of mice administrated 5-Fu and CP alone were 115% and 99%. Furthermore, survival rates of mice administrated 5-Fu and CP in combination with LC2782 were 226% and 244%, respectively.

• Journal of Microbiology and Biotechnology
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• 제33권8호
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• pp.1057-1065
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• 2023

Inflammatory bowel disease (IBD), a chronic inflammatory disease, results from dysregulation of the immune responses. Some lactic acid bacteria (LAB), including Lactobacillus, alleviate IBD through immunomodulation. In this study, the anti-colitis effect of LAB isolated from human breast milk was investigated in a mouse model induced acute colitis with 2,4,6-trinitrobenzene sulfonic acid (TNBS). TNBS remarkably increased weight loss, colon shortening, and colonic mucosal proliferation, as well as the expression levels of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1β. Oral administration of LAB isolated from human breast milk resulted in a reduction in TNBS-induced colon shortening, as well as induced cyclooxygenase (COX)-2, nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-κB). In addition, LAB suppressed inflammatory cytokines such as TNF-α, IL-6, and IL-1β, and thus showed an effect of suppressing the level of inflammation induced by TNBS. Furthermore, LAB alleviated gut microbiota dysbiosis, and inhibited intestinal permeability by increasing the expression of intestinal tight junction protein including ZO-1. Collectively, these results suggest that LAB isolated from human breast milk can be used as a functional food for colitis treatment by regulating NF-κB signaling, gut microbiota and increasing expression of intestinal tight junction protein.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.356-362
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• 2023

The 2'-fucosyllactose (2'-FL) is the richest components in a human milk oligosaccharide. Several studies have reported that 2'-FL has beneficial effects in infants. However, there are few studies on its immune-enhancing effects. This research aimed to examine the immune-enhancing effect of 2'-FL on immunosuppression by cyclophosphamide (CCP) in ICR mice. Mice were orally administered distilled water or 0.5 mg/kg B.W. 2'-FL for 14 days. An immunocompromised mouse model was induced using CCP 80 mg/kg B.W. at 12-14 days. Using the CCP had effects on reducing their body weight, organ weight, spleen index, natural killer (NK) cell activity, and cytokines concentration and expression. This study also used concanavalin A-mediated T-cell proliferation to verify the immune-enhancing effects in the sample. Body weight, spleen index, organ weight, and cytokine levels were measured to estimate the immune-enhancing effects. The body weight at 14 days tended to increase, and the spleen weight and index significantly increased in the 2'-FL group compared to the CCP group. The NK cell activity increased in the 2'-FL group compared to the CCP group, but there was no significant difference. The concentration of interleukin (IL)-2 tended to recover in the 2'-FL group compared to the CCP group. The 2'-FL group showed a significant increase of IL-10 and IFN-gamma concentration compared to the CCP group. In addition, there was a trend of increased IL-10 mRNA expression compared to the CCP group. These results revealed that 2'-FL improved CCP-induced immunosuppression, suggesting that 2'-FL may have the potential to enhance the immune system.

• Journal of Dairy Science and Biotechnology
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• 제38권2호
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• pp.112-120
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• 2020

본 연구는 Enterococcus faecalis EF-2001 사균체를 발효유에 첨가하였을 시 나타나는 생리활성을 검증하고자 실시하였다. 발효유는 같은 양의 starter만 넣은 일반발효유 NFM, E. faecalis EF-2001 사균체를 100 ㎍/mL의 농도로 첨가한 EFM1, E. faecalis EF-2001 사균체를 500 ㎍/mL의 농도로 넣은 EFM2를 제조하였다. E. faecalis EF-2001 사균체를 발효유 첨가하였을 때 나타나는 항염증 효과를 검증하기 위해 mouse 유래 대식세포인 RAW 264.7 cell을 이용하였다. RAW 264.7 cell을 이용한 세포독성 실험의 결과, 사균의 농도가 높아질수록 세포 생존율이 유의적으로 감소하였으며(p<0.05), 80% 이상의 세포 생존율을 가지는 농도인 5, 10, 15 ㎍/mL에서 실험을 진행하였다. Nitric oxide의 생성 억제능 측정결과는 LPS 처리군에 비해 발효유에서 NO 생성을 저해하는 경향을 나타냈다. 또한, 일반발효유보다 사균을 첨가한 발효유에서 NO 생성을 더욱 저해하는 결과를 나타내었다. Prostaglandin E₂의 억제율 측정결과도 15 ㎍/mL의 농도에서 PGE₂ 분비량을 감소시킴을 확인하였으며, 일반 발효유보다 사균이 첨가된 발효유에서 더욱 억제됨을 확인하였다. 따라서, 본 연구를 통해 E. faecalis EF-2001 사균체가 발효유에 첨가되어도 염증 매개물질인 NO 및 PGE₂의 생성을 감소시킴으로써 추후 사균체가 첨가된 발효유 제조 및 연구에 대한 기초 연구 결과로 활용될 수 있을 것으로 사료된다.

• 한국식품과학회지
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• 제38권2호
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• pp.176-182
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• 2006

본 연구는 축산농가에서 가축의 세균성 질병의 예방과 치료 및 성장촉진을 목적으로 주로 사용되고 있으며, 축산물과 유가공품에 잔류 가능성이 높은 sulfamethazine(SMZ)을 검출할 수 있는 직접경쟁 효소면역분석법(direct competitive ELISA)의 개발과 이를원유 및 시판우유 분석에 이용하는 것을 목적으로 하였다. 면역항원의 합성은 hemiglutarate와 hemisuccinate를 이용하여 SMZ-hemiglutarate(SMZ-HG)와 SMZ-hemisuccinate(SMZ-HS) hapten을 유도한 후 단백질 KLH와 STI를 결합시켜 마우스 면역에 사용하였고, SMZ-HG-KLH를 이용하여 면역한 마우스에서 가장 높은항체 생성정도를 나타내었다. 세포융합과 cloning을 실시하여 총15종의 hybridoma cell line을 확보하였고 그 중 가장 경쟁성이 뛰어나고 민감도가 높은 1H11-5 hybridoma를 선택하고 대량 생산하였다. 생산된 항체는 sulfamethazine에만 54%의 교차 반응성을 나타내었고, 다른 설파계 항생제와는 반응을 하지 않는 특이성이 높은 항체로 확인되었고 이를 이용하여 확립된 direct competitive ELISA법은 검출범위가 0.1-100 ppb 수준으로 기존에 보고된 ELISA보다 민감도가 높았다. 민감도와 특이성이 높은 direct competitive ELISA를 이용하여 원유와 시판우유를 분석하기 위한 전처리법을 확립하여 회수율을 확인한 결과 원유의 경우 82-121%까지 회수가 되는 것으로 나타났으며, 시판유의 경우는 82-97%까지 회수가 되는 것으로 나타나 우유 샘플 중에 미량 잔류하는 SMZ를 신속하고 정확하게 분석할 수 있을 것으로 사료되었다. 이러한 연구결과를 종합해 볼 때 확립된 direct competitive ELISA법은 우유 시료뿐만 아니라 모든 축산물에 잔류 가능성이 높은 SMZ의 분석을 신속하고 정확하게 분석이 가능할 것으로 사료되었고 확립된 direct competitive ELISA법의 안정화 조건을 확립하고 응용한다면 외국으로부터 생산 및 수입되는 ELISA kit을 대체할 수 있는 저가형 국산화 ELISA kit의 상용화가 가능할것으로 사료되었다.