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http://dx.doi.org/10.5713/ajas.2003.732

Anti-idiotypic Antibodies against Bovine Growth Hormone  

Verma, N.K. (Animal Biochemistry Division, National Dairy Research Institute)
Sodhi, R. (Animal Biochemistry Division, National Dairy Research Institute)
Rajput, Y.S. (Animal Biochemistry Division, National Dairy Research Institute)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.16, no.5, 2003 , pp. 732-737 More about this Journal
Abstract
Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [$I^125$ ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.
Keywords
Anti-idiotypic Antibodies; Bovine Growth Hormone; Bovine Somatotropin; Receptor Assay; Antibody Purification;
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