• Fisheries and Aquatic Sciences
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• 제25권11호
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• pp.559-571
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• 2022

Galectins, a family of ß-galactoside-binding lectins, have emerged as soluble mediators in infected cells and pattern recognition receptors (PRRs) responsible for evoking and regulating innate immunity. The present study aimed to evaluate the role of galectin-1 in the host immune response of redlip mullet (Liza haematocheilia). We established a cDNA database for redlip mullet, and the cDNA sequence of galectin-1 (LhGal-1) was characterized. In silico analysis was performed, and the spatial and temporal expression patterns in gills and blood in response to lipopolysaccharide polyinosinic:polycytidylic acid, and Lactococcus garvieae were estimated via quantitative real-time PCR. Functional assays were conducted using recombinant protein to investigate carbohydrate binding, bacterial binding, and bacterial agglutination activity. LhGal-1 was composed of 135 amino acids. Conserved motifs (H-NPR, -N- and -W-E-R) within the carbohydrate recognition domain were found in LhGal-1. The tissue distribution revealed that the healthy stomach expressed high levels of LhGal-1. The temporal monitoring of LhGal-1 mRNA expression in the gill and blood showed its significant upregulation in response to immune challenges with different stimulants. rLhGal-1 exhibited binding activity in response to carbohydrates and bacteria. Moreover, the agglutination of rLhGal-1 against Escherichia coli was observed. Collectively, our findings suggest that LhGal-1 may function as a PRR in redlip mullet. Furthermore, LhGal-1 can be considered a significant gene to play a protective role in redlip mullet immune system.

• Genomics & Informatics
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• 제20권4호
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• pp.45.1-45.7
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• 2022

Food security will be affected by climate change worldwide, particularly in the developing world, where the most important food products originate from plants. Plants are often exposed to environmental stresses that may affect their growth, development, yield, and food quality. Auxin is a hormone that plays a critical role in improving plants' tolerance of environmental conditions. Auxin controls the expression of many stress-responsive genes in plants by interacting with specific cis-regulatory elements called auxin-responsive elements (AuxREs). In this work, we performed an in silico prediction of AuxREs in promoters of five auxin-responsive genes in Zea mays. We applied a data fusion approach based on the combined use of Dempster-Shafer evidence theory and fuzzy sets. Auxin has a direct impact on cell membrane proteins. The short-term auxin response may be represented by the regulation of transmembrane gene expression. The detection of an AuxRE in the promoter of prolyl oligopeptidase (POP) in Z. mays and the 3-fold overexpression of this gene under auxin treatment for 30 min indicated the role of POP in maize auxin response. POP is regulated by auxin to perform stress adaptation. In addition, the detection of two AuxRE TGTCTC motifs in the upstream sequence of the bx1 gene suggests that bx1 can be regulated by auxin. Auxin may also be involved in the regulation of dehydration-responsive element-binding and some members of the protein kinase superfamily.

• 한국의상디자인학회지
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• 제25권2호
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• pp.97-110
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• 2023

The purpose of this study is to develop 3D pattern textile design of traditional Jogakbo motifs and fashion products using it. As a research method, first, through literature review, the three-dimensional representation of geometry on a plane with Jogakbo, design cases were examined. Second, through a survey, the purchase perception and design preference of Jogakbo cultural products was analyzed. Third, based on the results of the survey on color and print, the 3D pattern design for each type of Jogakbo is printed, and then textile fashion cultural products were developed. The results of this study are as follows. First, the reason why the public was not attracted to the purchase of cultural products was disatisfaction with practicality, unsuitable preference, price adequacy, aesthetics, and originality. Therefore, it was analyzed that quality, practicality, price, carry-on storage harmony and manageability, as well as aesthetic design were important factors for consumers. Second, the stereoscopic space on the plane expanded the two-dimensional plane space by forming a cube through the division and dissolution of geometry could be visualized using color expression of cubes of different brightness depending on the direction of light. Third, Jogakbo had eight types consisting of four detailed forms and three arrangement methods. The 3D pattern design could be developed through regular disolution and stereoscopic construction using Jogakbo's representative images for each type. In addition, it was found that it was easy to produce Jogakbo fashion products suitable for modern people through 3D pattern digital textile printing applying traditional colors.

• 한국의상디자인학회지
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• 제25권2호
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• pp.151-165
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• 2023

This study aims to develop a mechanic embroidery bag design that reflects the symbolic image of Korean independence activists who sacrificed for the country so that today's Korea can exist. As a research method, the theoretical consideration and image collection of independence activists were conducted through a literature review and visits to the memorial halls of six independence activists. The bag design was developed using mechanic embroidery textile of 12 motifs manufactured in previous studies through the computer mechanic embroidery sewing machine, and the types were limited to totes, Boston, clutches, baskets, shoppers, hover bags, and hat boxes. The results of this study are as follows. First, it was found that the dense light and shade and delicate color expression of each motif design were the most important and achieved through the proper selection and utilization of embroidery yarn according to the mechanic embroidery expression technique for developing in-depth textile materials. Second, unlike in the past, when machine embroidery was difficult to handle, the spread of embroidery CAD and computer machine embroidery sewing machines made it easier for individuals to develop embroidery products. Third, this study once again emphasized the symbolism of Korean independence activists who sacrificed for the nation to exist at a time when it needs to be recognized due to the lack of fashion and diversity in cultural products containing historical and cultural meaning and values of the country. This study was able to develop computer machine embroidery textiles and various bag designs using various mechanic embroidery expression techniques that can highlight the symbolic image of each motif.

• 영어영문학
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• 제55권3호
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• pp.383-398
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• 2009

Based on the Greek myth of Pygmalion and the fairy tale of Cinderella, Shaw's Pygmalion demonstrates a masterful coalescence of these two narrative motifs into a coherent plot scheme. Even more significant is his keen insight into the conflicts created at the tripartite intersection of human activity concerning language/class/culture, which, as the leitmotif, revolves around lessons in language learning. This play basically deals with human transformation and by its very nature, Higgins's experimentation with transforming Eliza cannot stop at language alone. Her cultural transformation ripples over into the realms of gesture and even a unique way of living (modus vivendi) intimately associated with taste and manners, which Bourdieu terms as habitus. By acquiring a new fashion and language, Eliza is reborn as a new lady aspiring to be filled with a newly acquired habitus. While separating her from her old Cockney style, Higgins inculcates Queen's English in Eliza, in which process her changed speech styles gradually transforms and restructures her deportment and manners, finally generating new practices, perceptions and attitudes. The gist of Pygmalion is however less Eliza's ascent into the middle class than her battle for symbolic capital waged at the level of language. By problematizing his contemporary practice of habitus conventionalized and warped by class distinctions based on economic, social and cultural capitals, Shaw creates a new humanist model of man founded on spiritual and rational virtues. In conclusion, Eliza is not a frigid Galatea but a dynamic character that goes through a brilliant transformation of three stages: 1) linguistic; 2) cultural, and 3) humanist. Finally she is built into a "consort battleship" on an equal standing with her sculptor. The process of her character-building cannot be illuminated without resorting to the dynamic notion of habitus, which highlights the process of inculcation, structuring, generation and transposing. Given the overwhelming weight of the heroine's role and the dynamic process of her transformation as the major plot scheme, this play should be christened Galatea in lieu of Pygmalion.

• 영어영문학
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• 제55권4호
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• pp.641-659
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• 2009

Since Plath killed herself in 1963, the theme of death has become one of the central motifs and allusions in her work. The biographical emphasis continues to blur the boundary between the artistic world and the material world. While approaching Plath's work from the perspective of personal experience, the objective of this paper is not to suggest that we encounter Plath's personal voice and emotions directly in her work. Rather, I emphasize how Plath's work of mourning is substantiated in the act of writing. Plath protects herself from the unnamable or the existential void by writing poems. She shows the way in which art or writing enables the subject to confront traumatic memory. While the death drive propels Plath towards destruction, artistic formation serves to alleviate her psychic crisis. What I shall suggest in the paper is how works of art lead the melancholic subject to challenge traumatic events. Plath herself suggests the therapeutic power of language. Plath's hostility toward women as well as men situates her work nearer to the Kristevan psychoanalytic theory which examines depressive anxieties intrinsically linked to the loss of maternal objects. Kristeva's particular focus on the concept of "death-bearing mother" or the unnamable offers a fruitful reading of the representation of infantile fantasies, sexuality, anger, and ambivalence toward lost loved object which clearly dominates most of Plath's poems. Kristeva elaborates mourning and melancholia through the framework of signification and it is of especial relevance in deciphering the recurring death drive and melancholic rage in Plath's work. Melancholic subjects in Plath's work are characterized by an amorphous state, occupying a borderline state regulated by the death drive.

• 한국초지조사료학회지
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• 제43권3호
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• pp.168-176
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• 2023

Silicon (Si) has the potential to improve plant growth and stress tolerance. The study aimed to explore Si-involving plant responses and molecular characterization of different Si-responsive genes in alfalfa. In this study, the exogenous supplementation of Si enhanced plant growth, and biomass yield. Si-acquisition in alfalfa root and shoot was higher in Si-supplemented compared to silicon deficient (-Si) plants, implying Si-acquisition has beneficial on alfalfa plants. As a consequence, the quantum efficiency of photosystem II (Fv/Fm) was significantly increased in silicon-sufficient (+Si) plants. The quantitative gene expression analysis exhibited a significant upregulation of the Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes in alfalfa roots, while BOR1, BOR4, NIP2, and NIP3 showed no significant variation in their expression. The MEME results further noticed the association of four motifs related to the major intrinsic protein (MIP). The interaction analysis revealed that NIP5;1 and Lsi1 showed a shared gene network with NIP2, BOR1, and BOR4, and Lsi2, Lsi3 and NIP3-1, respectively. These results suggest that members of the major intrinsic proteins (MIPs) family especially Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes helped to pass water and other neutral solutes through the cell membrane and those played significant roles in Si uptake and transport in plants. Together, these insights might be useful for alfalfa breeding and genome editing approaches for alfalfa improvement.

• Journal of Life Science
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• 제11권2호
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• pp.111-115
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• 2001

This study was designed to clone the SNF2/SW12 helicase-related genes from the fission yeast Schizosaccha-romyces pombe and thereafter to elucidate the common functions of the proteins in this family. The $hrp^{2+}$gene was cloned by polymerase chain reaction amplification using degenerative primers from conserved SNF2 motifs within the ERCC6 gene, which encodes a protein involved in DNA excision repair. Like other SNF2/SW12 family proteins, the deduced amino acid sequence of Hrp2 contains DNA-dependent ATPase/7 helicase domains as well as the chromodomain and the DNA binding domain. This configuration is similar to that of mCHD1 (mouse chromo-ATPase/helicase-DNA-dinding protein 1), suggesting that Hrp2 is a S. pombe homolog of mCHD1, which is thought to function in altering the chromatin structure to control the gene expression. To characterize the function of Hrp2, 4 Uracil-Hrp2 fusion protein, it was purified near homogeneity by affinity chromatography on $Ni^{2+}$-NTA agarose, DEAE-Sepharose ion exchange arid Sephacryl S-200 gel filtration chromatographies. The purified fusion protein exhibited DNA-dependent ATPase activity, which was stimulated by both double-stranded and single-stranded DNA. To determine the steady-state level of $hrp^{2+}$ transcripts during growth, cells were cultured in medium and collected at every 2hr to prepare total RNAs. The northern blot analysis showed that the level of $hrp^{2+}$ transcripts reached its maximum before the cells entered the exponential growth phase and then decreased gradually, This result implies that Hrp2 may be required at early stages of cell growth.h.

• 문화기술의 융합
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• 제9권6호
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• pp.113-122
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• 2023

본 연구는 문화 & 소프트 파워(soft power)가 주도하는 지식정보사회에 있어서 전통문화의 유물자산을 활용한 문화상품 개발을 전제로 진행하였다. 통일신라시대 전통건축에서 출토된 와당 유물의 문화콘텐츠 상품 가능성을 탐색하고 주얼리 디자인에 활용성이 높은 상품화 모티브의 확장성을 넓히는 맥락에서 진행되었다. 먼저 문헌과 매체 연구를 통하여 와당의 원형과 소재, 용도, 크기, 의미, 조형적 심미성을 파악하였다. 고찰된 와당중에서 카테고리별 대표성과 모듈·패턴화 가치가 있는 10종류의 와당을 선택하여 '형태적 단순화 기법'으로 원형이미지를 추출·모듈화하였다. 문양구성 배열형식 체계화 방법인 '수학적 대칭성 분석기법'에 근거하여 문화콘텐츠산업 및 주얼리디자인 개발시에 활용할 수 있는 평면적 조형요소를 도출하였다. 향후 주얼리산업에서의 활용성 확대를 위하여 2D 디지털이미지로 제시하였다. 향후에도 전통문화를 매개로 하는 다양한 문화콘텐츠산업에 관한 연구가 더한층 활성화되기를 기대한다.

• 한국결정성장학회지
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• 제34권1호
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• pp.22-29
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• 2024

조류를 모티브로 디자인한 작품은 예나 지금이나 꾸준히 인기를 끌고 있다. 특히 신화에 나오는 조류를 모티브로 디자인 작품들이 큰 사랑을 받고 있다. 그래서 본 연구는 중국 신화의 대표작인 산해경 중에서 조류를 모티브로 한 주얼리 디자인을 연구하고자 한다. 본 연구에서는 산해경에서 등장한 조류의 특징을 분석하였으며, 그 조류들을 모티브로 한 디자인 사례를 조사하였다. 산해경에 등장하는 조류는 크게 신조(divine birds)와 괴조(monstrous birds)로 구분되며, 신조는 주로 다양한 색상의 조합 및 통일된 색조로, 괴조는 주로 대비가 강한 적색, 녹색 및 흰색으로 표현하고 있다. 산해경 속의 조류를 모티브로 한 장신구의 형태, 색상 및 상징성을 분석했다. 이러한 작품들은 주로 다양한 색상(multicolored color), 녹색 및 적색을 사용했다. 시대 배경 및 디자인 형태의 관련성을 통해 산해경의 기원과 관련된 시대에서 형태와 패턴을 적용하여 디자인했다. 이러한 연구 결과를 바탕으로 신화적인 조류 디자인에 관심을 가지는 디자이너들에게 도움이 될 것으로 기대한다.