• 한국의상디자인학회지
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• 제21권4호
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• pp.127-137
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• 2019

Nail art, which has been popularized recently, has increased convenience in the direction of nail art design harmonized with clothing and the increased interest and usage of nail art decoration. This study set out to propose practical planar and three-dimensional nail art designs in harmony with modern clothes by applying trimmings common in 18th century women's clothing, which used a variety of attachable decorations. As for the methodology, the investigator examined theories in previous studies, literature, and analyzed the types of trimming in female portraits from the 18th century at the museum at the Palace of Versailles in France. Planar and three-dimensional nail art designs were created for each of the top three trimming types- braids, laces, and ribbons, which were identified through analysis. The study applied the trimmings of the 18th century women's clothes to nail art design and had the following results: First, the top three trimming types were identified based on the analysis of the 18th century portraits. They were then used as motifs in the production of planar and three-dimensional nail art designs. The results show that the motifs of the clothes trimmings were fit for and had practical possibilities for expression in nail art designs. Secondly, diversity and the expansion of ideas in the study of nail art design were promoted. In addition, elements were increased to have mutually complementary effects with clothes. Finally, the study made various attempts at nail design methods by adopting a range of nail art expression techniques including hand painting, 2D & 3D art techniques, and using planar and three-dimensional nail decorations, thus proposing a positive direction for the organization of a curriculum to make use of 18th century women's clothing motifs in nail art education.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.315-325
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• 2016

A novel esterase gene, est7K, was isolated from a compost metagenomic library. The gene encoded a protein of 411 amino acids and the molecular mass of the Est7K was estimated to be 44,969 Da with no signal peptide. Est7K showed the highest identity of 57% to EstA3, which is an esterase from a drinking water metagenome, when compared with the enzymes with reported properties. Est7K had three motifs, SMTK, YSV, and WGG, which correspond to the typical motifs of family VIII esterases, SxxK, Yxx, and WGG, respectively. Est7K did not have the GxSxG motif in most lipolytic enzymes. Three additional motifs, LxxxPGxxW, PLGMxDTxF, and GGxG, were found to be conserved in family VIII enzymes. The results of the phylogenetic analysis and the alignment study suggest that family VIII enzymes could be classified into two subfamilies, VIII.1 and VIII.2. The purified Est7K was optimally active at 40ºC and pH 10.0. It was activated to exhibit a 2.1-fold higher activity by the presence of 30% methanol. It preferred short-length p-nitrophenyl esters, particularly p-nitrophenyl butyrate, and efficiently hydrolyzed glyceryl tributyrate. It did not hydrolyze β-lactamase substrates, tertiary alcohol esters, glyceryl trioleate, fish oil, and olive oil. Est7K preferred an S-enantiomer, such as (S)-methyl-3-hydroxy-2-methylpropionate, as the substrate. The tolerance to methanol and the substrate specificity may provide potential advantage in the use of the enzyme in pharmaceutical and other biotechnological processes.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.905-913
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• 2014

Lj-RGD3, an RGD (Arg-Gly-Asp) toxin protein from the salivary gland of Lampetra japonica, exhibits antifungal activity against Candida albicans. Lj-RGD3 has three RGD motifs and shows homology to histidine-rich glycoprotein. We synthesised two mutant derivatives of Lj-RGD3: Lj-26, which lacks all three RGD motifs and contains no His residues; and Lj-112, which lacks only the three RGD motifs. We investigated the effects of the wild-type and mutated toxins on a gram-positive bacterium (Escherichia coli), a gram-negative bacterium (Staphylococcus aureus), and a fungus (C. albicans). rLj-RGD3 and its mutants exhibited antifungal but not antibacterial activity, as measured by a radial diffusion assay. The C. albicans inhibition zone induced by rLj-112 was larger than that induced by the other proteins, and its inhibitory effect on C. albicans was dose-dependent. In viable-count assays, the rLj-112 MIC was $7.7{\mu}M$, whereas the MIC of the positive control (ketoconazole) was $15{\mu}M$. Time-kill kinetics demonstrated that rLj-112 effectively killed C. albicans at $1{\times}$ and $2{\times}$ MIC within 12 and 6 h, respectively. Electron microscopy analysis showed that rLj-RGD3 and rLj-112 induced C. albicans lysis. Our results demonstrate a novel anticandidal activity for rLj-RGD3 and its mutant derivatives.

• 복식
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• 제61권10호
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• pp.42-54
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• 2011

The study was focused on surveying the cultural identity and the origin of T-shirts, and examining their functions. Six cities were selected to categorize the design motifs of cultural identity appearing in the design of contemporary T-shirts. These cities include a city in France, United Kingdom, Italy, United States and Japan where collections are currently presented at, and also a city in China that has shown a high economic growth as one of the most popular sightseeing cities. Cultural identity was presented in the T-shirts that were examined in this study through diversified design motifs. Motifs used in T-shirt designs to reflect cultural identity included national flags, notable places, letters, names of geographic places, and individuals. A national flag is a motif that signifies national identity and it was used in the front of T-shirts as it is or was just partially used. Also, many world-renowned structures or architectural buildings were applied to T-shirt design. Noted places such as the White House, the Statue of Liberty in the U.S., Tienanmen Square in China and the Eiffel Tower in France were used. The unique characters of language, such as Hangeul in Korea were used as design elements to reflect the structural characteristics of letters. Names of geographic places were used as design elements through the disclosure of the name of a symbolic city or a tourist site in each country. In terms of figures, not only the historical figures or celebrities from each country were used, but the image of bodies and faces that represent each culture were used as well. In addition, this study was used to develop T-shirt designs that reveal the cultural identity of Korea.

• 패션비즈니스
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• 제28권1호
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• pp.36-50
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• 2024

This study aims to explore the potential of integrating traditional Indian motifs into modern textile design that appeals to the MZ generation. The objective is to create patterns that reflect the rich cultural tapestry of India while aligning with contemporary sensibilities. To achieve this, the design process includes a comprehensive literature review on the historical background, symbolism, and structure of Indian textiles and a survey to determine if the developed design suits the preferences of the MZ generation. Digital design tools such as TexPro, Photoshop, and Illustrator were used to create the designs based on the theme 'Cosmic Dream,' with sub-themes inspired by nature and Indian symbolism. 'Blooming Cosmos' focused on the structural beauty of the lotus, combined with paisley and rangoli patterns, for a vintage yet modern appeal. 'Cosmos in Blossom' is drawn from the rangoli technique of using varied materials, resulting in a romantic pastel-themed pattern that aligns with the 2023 Youth Trend. 'Cosmic Peacock' presented a lively ethnic pattern by merging simplified lotus motifs with India's national bird, peacock along with a paisley backdrop. A questionnaire was conducted to check the MZ consumer responses, and the most preferred design was the peacock and rangoli mixed design, ground colored in cream. These findings indicate the potential of the developed ethnic designs to be used for various purposes. By combining traditional motifs of India with modern elements, this study proposes ethnic textile designs that are suitable for MZ generation consumers.

• 복식
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• 제35권
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• pp.17-44
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• 1997

Medieval European practical art and style of costume is studied through referring to the literatures. The type of clothing that was seen on tapestries arts and crafts paintings and wall paintings and appeared in the architecture such as church. There were symbolic aspects of color motifs at this period and some restraints for clothing according to the social class, It was a period that draped garments due to the War of Crusades is appeared. Be-cause the medieval taste and decorative character is not only 'formative art' but also the taste beyoud 'mode' and atomsphere of that times it could be seen the strong reflec-tion of customs in the clothing of that times which is shown in works of art. 1) The purpose of Christian art which was found in mosaics stained glasses wall pain-tings and statues of architecture was not just the revival of natural phenomena but visuali-zation of sacred and invisible things. It was valuable to understandin of the spiritual through the sense organ It was a monumental art that was combined with decorative func-tion and role of the Bible. It revealed what was about the religious spiritual miracle, 2) In the medieval European painting characteristic and beautiful creativity was the basis of an argument. Both "Worship of East-ern" that is painted ion a parchment and "Cor-onation of Charles VI" were described decor-ation motifs on the edge of buildings geo-metric patterns and others with outstanding skill. there were precise technologic skill of architect and glassmen and lots of patience of craftsmen. " The Labour of the Months" and the scene of"A Trial at the Court" is also de-scribed elaborately. 3) Tapestry was developed in France Ger-many Swiss at the 14th century. Religionary and historical themes unicorn lady bird flowering plants and others were major motifs. It was very important as decorative wall coverings and as practical door arch and bed hangings. It was made through patient hard work with simple technique and it was con-sidered as an art for practical use. Tapestry was what everyone wish to have. It is reckoned for the item of the best gift. The royalty and nobility ordered and used custom-made tapestries. Sometimes the subject of motifs consisted of series which were deeply related to living 4) Decorative arts and crafts was the art that used materials such as precious metals jewels and others and that accorded with the luxurious and gorgeous taste of the royalty and nobility. Christian considered splendid and beautiful color of light as a symbol of glory. They used also for church appliances, There were metal crafts wood crafts textile crafts and others, As was stated above the costume appeared on the arts and crafts for living revealed the process of changes saw how the politic econ-omic and social organizations were developed.and social organizations were developed.

• Molecules and Cells
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• 제38권3호
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• pp.259-266
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• 2015

The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.

• Asian-Australasian Journal of Animal Sciences
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• 제32권5호
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• pp.614-628
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• 2019

Objective: The inhibitory leukocyte immunoglobulin-like receptors (LILRBs) play an important role in innate immunity. The present study represents the first description of the cloning and structural and functional analysis of LILRB1 and LILRB3 isolated from two genetically disparate chicken lines. Methods: Chicken LILRB1-3 genes were identified by bioinformatics approach. Expression studies were performed by transfection, quantitative polymerase chain reaction. Signal transduction was analyzed by western blots, immunoprecipitation and flow cytometric. Cytokine levels were determined by enzyme-linked immunosorbent assay. Results: Amino acid homology and phylogenetic analyses showed that the homologies of LILRB1 and LILRB3 in the chicken line 6.3 to those proteins in the chicken line 7.2 ranged between 97%-99%, while homologies between chicken and mammal proteins ranged between 13%-19%, and 13%-69%, respectively. Our findings indicate that LILRB1 and LILRB3 subdivided into two groups based on the immunoreceptor tyrosine-based inhibitory motifs (ITIM) present in the transmembrane domain. Chicken line 6.3 has two ITIM motifs of the sequence LxYxxL and SxYxxV while line 7.2 has two ITIM motifs of the sequences LxYxxL and LxYxxV. These motifs bind to SHP-2 (protein tyrosine phosphatase, non-receptor type 11) that plays a regulatory role in immune functions. Moreover, our data indicate that LILRB1 and LILRB3 associated with and activated major histocompatibility complex (MHC) class I and ${\beta}2-microglobulin$ and induced the expression of transporters associated with antigen processing, which are essential for MHC class I antigen presentation. This suggests that LILRB1 and LILRB3 are transcriptional regulators, modulating the expression of components in the MHC class I pathway and thereby regulating immune responses. Furthermore, LILRB1 and LILRB3 activated Janus kinase2/tyrosine kinase 2 (JAK2/TYK2); signal transducer and activator of transcription1/3 (STAT1/3), and suppressor of cytokine signaling 1 genes expressed in Macrophage (HD11) cells, which induced Th1, Th2, and Th17 cytokines. Conclusion: These data indicate that LILRB1 and LILRB3 are innate immune receptors associated with SHP-2, MHC class I, ${\beta}2-microglobulin$, and they activate the Janus kinase/signal transducer and activator of transcription signaling pathway. Thus, our study provides novel insights into the regulation of immunity and immunopathology.

• 공연문화연구
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• 제40호
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• pp.37-69
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• 2020

이 연구는 한국 여성영화의 흐름을 통해, 그 특징과 미학 그리고 유사성과 차이점을 살피기 위해 구상된 연구이다. 이 연구를 시행하기 위해서 시대별 대표성을 간직한 네 편의 영화를 선택해야 했다. 일제 강점기에 제작된 영화 <미몽>, 1950년대 여성주의에 대한 논쟁을 대대적으로 촉발한 <자유부인>, 여성의 황폐해진 삶과 이율배반적 남성 시각을 결합한 1990년대 영화 <은마는 오지 않는다>, 그리고 한국 페미니즘 영화의 미래 전형을 제시한 <바람난 가족>이 그 대상 작품이다. 특히 이러한 작품들은 시대별 한국 영화의 전형성과 대표성을 담보하고 있다는 점에서 주목되는 대상작이 아닐 수 없다. 이를 바탕으로 이 작품들에 나타나는 두 개의 공통 모티프를 집중적으로 조명했다. 하나는 여성의 영어 된 처지를 상징적으로 보여주는 '새장' 모티프이고, 다른 하나는 여성들이 자신의 처지를 자각하고 현재 상황을 점검하는데 필요한 '거울' 모티프이다. 한국의 여성영화는 '새장'과 '거울'의 모티프를 일정 부분 공유하고 있을 뿐만 아니라, 이러한 모티프의 연계를 통해 궁극적으로 겨냥했던 작가적 전언을 전달하는 데에도 주력해왔다.

• 문화기술의 융합
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• 제9권3호
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• pp.407-413
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• 2023

본 연구는 디지털 패션디자인 영역이 확장되고 있는 현시점에서 자수 특유의 시각적 표현효과를 보다 쉽고 다양하게 의복 디자인에 접목할 수 있는 기초자료를 제공하고자 자수 디자인의 디지털화에 의한 시각적 표현 효과가 실제 의상과 가상의상에서도 동일한 효과를 나타내는지 비교하였다. 컴퓨터 자수기를 이용한 실제 자수 모티브와 디지털화에 의한 DTP 자수 모티브, 디지털 모티브를 생성하였으며 의복에서의 시각적 표현효과를 비교하고자 각각의 자수 모티브가 들어간 패치포켓 형태의 T-셔츠를 제작하였다. 자수기로 제작한 자수가 삽입된 의복과 디지털화된 자수 모티브를 디지털텍스타일프린팅하여 제작된 의복의 시각적 효과를 비교한 결과, 색상(3.5), 질감(4.0), 광택(3.8), 두께(3.5)에서 자수원사의 컬러와 두께감 등 시각적으로 충분히 자수의 질감, 광택의 표현 효과를 나타낼 수 있음을 알 수 있었다. 가상의복의 자수 표현효과에 있어서도, 색상(3.8), 질감(4.3), 광택(3.9), 두께(3.6)의 높은 일치도를 보여, 우리는 디지털화된 자수모티브도 기존 자수 특유의 시각적 표현 효과를 충분히 구현할 수 있는 도구임을 확인할 수 있었다.