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Transgenic Mutagenesis Assay to Elucidaate the Mechanism of Mutation at Gene Level (유전자수준에서 돌연변이 유발기전을 밝히는 Transgenic Mutagenesis Assay)

  • Ryu, Jae-Chun;Youn, Ji-Youn;Cho, Kyung-Hae;Chang, Il-Moo
    • Environmental Mutagens and Carcinogens
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    • v.18 no.1
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    • pp.15-21
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    • 1998
  • Transgenic animal and cell line models which are recently developed and used in toxicology fields combined with molecular biological technique, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Transgenic models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease processes. The lac I and lac Z gnee most widely used as a mutational target in transgenic systems. The assay is performed by treatment with putative mutagenic agents, isolation of genomic DNA from cells or tissues, exposure the isolated DNA to in vitro packaging extract, plating and sequencing. The results from these processes provide not only mutant frequency as quantitative evaluation but also mutational spectrum as qualitative evaluation of various agents. Therefore we introduce and review the principle, detailed procedure and application of transgenic mutagenesis assay system in toxicology fields especially in mutagenesis and carcinogenesis.

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Influencing factors on satisfaction of field instructor in ambulance ride practice of paramedic students (응급구조(학)과 학생의 구급차동승실습 지도자 만족도에 영향을 미치는 요인)

  • Choi, Eun-Sook
    • The Korean Journal of Emergency Medical Services
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    • v.17 no.3
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    • pp.101-113
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    • 2013
  • Purpose: The purpose of this study was to provide an appropriate direction for effective field practice program by analyzing the influencing factors on satisfaction of field instructor in ambulance ride practice of paramedic students in Korea. Methods: The questionnaires were filled out by 204 paramedic students from July 22 to September 13, 2013. The collected data were analyzed with SPSS WIN 19.0 program. Results: The stepwise multiple regression analysis revealed that the most powerful predictor of satisfaction of field instructor was sincerity of directions. A combination of sincerity of directions (38.2%), achievement(15.4%), improvement of knowledge(3.9%) 4-week field practice(3.0%), 2 week field practice(1.6%), 4 grade(1.8%) pre-expectation of field practice(1.8%) accounted for 65.6% of the variance in paramedic students. Conclusion: We proposed the effective field practice program as well as sincerity of directions of field instructors. It is necessary to discuss before ambulance ride practice within the faculty and the field instructors.

A Study on the e-Business Marketing Strategy of Digital Age (디지털 시대의 효율적인 e비지니스 마케팅 전략에 관한 연구)

  • 조원길
    • The Journal of Information Technology
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    • v.3 no.4
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    • pp.89-105
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    • 2000
  • Electronic commerce includes the tasks that support the buying and selling of goods and services, and interactions among those tasks. Electronic commerce enables companies to close stores, reduce inventory requirements, and distribute products over the internet. Electronic commerce can simplify communication and change relationships The expanding global e-marketplace has triggered an evolution with powerful implications. The introduction of interactive media and the online environment has made real-time, customized one-to-one advertising, marketing and commerce possible. I-marketing is the most rapidly growing channel being explored today. Users of this expanding medium include a range of service providers, publishers, marketers of music, s/w, other products that cross an increasing number of industry lines. Thus, the opportunities for e-business marketing strategy, and new businesses based on interactivity and sophisticated database technology are vast. This study explores the e-business marketing strategy of digital age. Strategic e-marketing can harness the power of today's technology to propel a company of any size farther into the worldwide market than ay any other time in history, increasing potential profits and productivity.

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The Development of Mobile Applications to Attract Customers in a Continuous Rewards (연속적인 리워드로 고객을 유치하는 모바일 어플리케이션의 개발)

  • Lim, JinSeop;Shim, Jeachang
    • Journal of Korea Multimedia Society
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    • v.19 no.5
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    • pp.948-956
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    • 2016
  • Many franchise stores are in the surrounding. These stores offer many membership services. But, small traders do not provide much customer management service. For example, non-franchised cafes provide the services through paper coupons. However, most of paper coupons are available only in one store that issued the coupon. Besides, these coupons are in stamp format. Due to the absence of customers' management service, it is hard for small traders to attract customers compared to the franchise. Therefore, in this paper, We had implemented a mobile application, and applied to new customer management service in order to increase the price competitiveness of small traders. This service issues electronic coupon through a mobile app. customers can receive a relatively large amount of discount, and a deadline of coupons are short. When customers use coupons, new coupons are issued at the same time. This structure can be powerful means to lead customers' frequent visit. Small traders can gain a lot of regular customers by using this service.

A Method of Determining Whether a Smart-Phone is Moving (스마트폰이 이동 중인지 아닌지를 판단하는 방법)

  • Yim, Jae-Geol;Lee, Gye-Young;Shim, Kyu-Bark;Le, Thanh Cong
    • Journal of Korea Multimedia Society
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    • v.15 no.5
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    • pp.632-638
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    • 2012
  • A smartphone is one of the best candidate devices on which context based services can be provided because a smartphone has considerably powerful computing ability and large memory capacity and it is a personal belonging. One of the most important factors of context is the moving status of the smartphone. For example, if the smartphone is not moving and the user is in an exhibition room then we can conclude that the user is watching an exhibit. This paper proposes an algorithm of determining the moving status of a smartphone for the first time. Our test results showed that the proposed algorithm is 100% correct.

Design of OTP based on Mobile Device using Voice Characteristic Parameter (음성 특징 파라메터를 이용한 모바일 기반의 OTP 설계)

  • Cha, Byung-Rae;Kim, Nam-Ho;Kim, Jong-Won
    • Journal of Advanced Navigation Technology
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    • v.14 no.4
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    • pp.512-520
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    • 2010
  • As the applications based on Mobile and Ubiquitous becoming more extensive, the communication security issues of those applications are appearing to be the most important concern. Therefore, every part of the system should be thoroughly designed and mutually coordinated in order to support overall security of the system. In this paper. we propose new technique which uses the voice features in order to generate Mobile One Time Passwords(OTPs). Voice is considered to be one of the powerful personal authentication factors of biometrics and it can be used for generating variable passwords for one time use. Also we performed a simulation of homomorphic variability of voice feature points using dendrogram and distribution of voice feature points for proposed password generation method.

A Study on Social Carrying Capacity by Normative Approach of Perceived Crowding (혼합지각의 규범적 접근에 의한 사회적 수용능력에 관한 연구 : - 내장산국립공원 단풍이용객 대상의 사례연구 -)

  • Park Chung-In
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.1 s.108
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    • pp.10-18
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    • 2005
  • The main concept of social carrying capacity is based on the principle that 'there are limits to the number of users a given recreation site can accommodate in order to provide quality of recreation experience'. The quality of the experience is revealed as user's satisfaction and perceived crowding. In this respect, studies of social carrying capacity have been frequently conducted by measuring perceived crowding and satisfaction. The purpose of this study is to identify the variables affecting perceived crowding, and causality bet-ween satisfaction and perceived crowding. Four hundred seventy six visitors were selected at Naejangsan National park on peak day of autumn excursion through on-site survey. The collected data were applied by factor analysis for categorizing the research variables, and multiple regression for finding the causality among variables. The study results are follows. The expectation of crowding, as a normative variable for perceived crowding, are categorized by three factors; circulation, user facility, and landscape. The circulation factor is the most powerful affecting perceived crowding among three factor. The landscape factor, however, do not have statistical significance on perceived crowding. The causality between satisfaction and perceived crowding is found. Although this relation is significant in statistics, magnitude of the coefficient is very small. It might be interpreted that satisfaction cannot be predicted simply from a user's perception of crowding, but a multi-dimensional concept such as adaptation and coping behavior.

The Possession and Space Usage of Furniture and Housing Goods by the Apartment Dweller - In case of Apartment in Chonju - (아파트 거주자의 가구와 주거용품의 소유 및 공간사용 - 전주시를 중심으로 -)

  • 이화실
    • Journal of the Korean housing association
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    • v.5 no.2
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    • pp.99-108
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    • 1994
  • The purpose of this study was to present basic data for better space plan of the apartment housing. It attempted to examine the possession and space usage of furniture and housing goods in three type floor plan. It also studied in relation to socidemographic variables and physical variables. The major findings were as follows :1) Housewife age, educational level, family income, number of family members were affected variables to the possession of housing goods and furniture except furniture possession to housewife age.2) It appeared similarity in the furnishing of housing goods and furniture in case of same floor plan.3) Generally, the space of living room was used often in furnishing of furniture and housing goods. 4) Linear arrangement of sofa set and corner arrangement of Anbang furniture are a tendency to get a effective space for activities in living room.5) The size of floor plan space was the most powerful variable to explain the possession and space usage of housing goods and furniture.6) The scarce of space result in pressing the household living space hard. The storage space should be planned and chosen after consideration of human factors according to the tastes, character of the users, and the space factors according to the composition of unit plan and the size of each living space. In case of the small size plan, particularly, it needed to the Built-in furniture as a effective method.

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Generation of Simulation input Stream using Threshold Bootstrap (임계값 부트스트랩을 사용한 시뮬레이션 입력 시나리오의 생성)

  • Kim Yun Bae;Kim Jae Bum
    • Korean Management Science Review
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    • v.22 no.1
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    • pp.15-26
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    • 2005
  • The bootstrap is a method of computational inference that simulates the creation of new data by resampling from a single data set. We propose a new job for the bootstrap: generating inputs from one historical trace using Threshold Bootstrap. In this regard, the most important quality of bootstrap samples is that they be functionally indistinguishable from independent samples of the same stochastic process. We describe a quantitative measure of difference between two time series, and demonstrate the sensitivity of this measure for discriminating between two data generating processes. Utilizing this distance measure for the task of generating inputs, we show a way of tuning the bootstrap using a single observed trace. This application of the threshold bootstrap will be a powerful tool for Monte Carlo simulation. Monte Carlo simulation analysis relies on built-in input generators. These generators make unrealistic assumptions about independence and marginal distributions. The alternative source of inputs, historical trace data, though realistic by definition, provides only a single input stream for simulation. One benefit of our method would be expanding the number of inputs achieving reality by driving system models with actual historical input series. Another benefit might be the automatic generation of lifelike scenarios for the field of finance.

A Study on the Sound Quality of Passenger Cars (자동차 실내 소음의 음질 연구)

  • 김정우;최종대;허정기;변언섭
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1998.04a
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    • pp.94-101
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    • 1998
  • This paper presents the psychoacoustic analyses on the vehicle interior, noise generated when accelerating. Following the objective quantification for vehicle noises of different classes, the subjective decision process by the jury test is adopted to interrelate these results based on two different approaches. In particular, the paired comparison method is employed in assessing the sound quality in terms of the quietness, the powerfulness, and the pleasantness of the human auditory sensation. Special attention is paid to the physical interpretation of psychoacoutic parameters and their interrelation to the subjective assessment scores. In order to ensure the integrity of statistics of the survey, various tests are performed. The results show that the consistency of the "quiet" sensation is far higher than that of the "powerful" and the "pleasant" sensations. Also, it is found that the most significant parameter determining these feelings turns out to be the inherent loudness of the noise instead of the conventional indicators such as the weighted sound pressure level. It may be concluded that the psychoacoustic analysis offers more realistic evaluation for vehicle noise and assists the improvement of NVH more efficiently.ement of NVH more efficiently.

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