• Journal of Ginseng Research
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• 제45권3호
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• pp.401-407
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• 2021

Background: Gintonin is an exogenous ginseng-derived G-protein-coupled lysophosphatidic acid (LPA) receptor ligand. LPA induces in vitro morphological changes and migration through neuronal LPA1 receptor. Recently, we reported that systemic administration of gintonin increases blood-brain barrier (BBB) permeability via the paracellular pathway and its binding to brain neurons. However, little is known about the influences of gintonin on in vivo neuron morphology and migration in the brain. Materials and methods: We examined the effects of gintonin on in vitro migration and morphology using primary hippocampal neural precursor cells (hNPC) and in vivo effects of gintonin on adult brain neurons using real time microscopic analysis and immunohistochemical analysis to observe the morphological and locational changes induced by gintonin treatment. Results: We found that treating hNPCs with gintonin induced morphological changes with a cell rounding following cell aggregation and return to individual neurons with time relapses. However, the in vitro effects of gintonin on hNPCs were blocked by the LPA1/3 receptor antagonist, Ki16425, and Rho kinase inhibitor, Y27632. We also examined the in vivo effects of gintonin on the morphological changes and migration of neurons in adult mouse brains using anti-NeuN and -neurofilament H antibodies. We found that acute intravenous administration of gintonin induced morphological and migrational changes in brain neurons. Gintonin induced some migrations of neurons with shortened neurofilament H in the cortex. The in vivo effects of gintonin were also blocked by Ki16425. Conclusion: The present report raises the possibility that gintonin could enter the brain and exert its influences on the migration and morphology of adult mouse brain neurons and possibly explains the therapeutic effects of neurological diseases behind the gintonin administration.

• 한국환경과학회지
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• 제6권1호
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• pp.53-60
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• 1997

The toxicity of purified ricln from Ricinus communis to rats was examined by histological and histochemical methods. Sprague-Dawley rats were injected intraperitoneally with 75$\mu\textrm{g}$/kg body weight of ricin and were sacrified at itntervals of 6, 24, 48 and 120 hours after injectoon. The major morphological changes, such as cloudy swelling, hydropic degeneration, necrosis, fatty change, blood congestion, increase of Kupffer cells in number and extension of sinusoids, were obvious in the liver of experimental group. These morphological changes of hepatic cells were mainly observed in both the periportal and midlobular region of hepatic lobule. The extension of sinusoids was obvious in the controlobular region. And glycogen dlstrlbution of hepatic cells tended to decrease in the same region showing morphlogical changes as compared with the control group.

• 대한의용생체공학회:의공학회지
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• 제26권6호
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• pp.383-391
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• 2005

Age-related White Matter Changes (WMC) on Magnetic Resonance Imaging (MRI) are known to appear frequently in Multiple sclerosis (MS) and Alzheimer's disease and to be related to cognitive impairment. The characterization of these WMC is very important to the study of psychology and aging. These changes consist of periventricular and subcortical types, however it is difficult to detect and segment WMC using only intensity-based methods, because their intensity, level IS similar to th~t of the gray matter (GM). In this paper, we propose a new method of segmenting periventricular WMC using K-means clustering and morphological features.

• Journal of Plant Biology
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• 제6권1호
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• pp.1-7
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• 1963

Two individuals of intergeneric hybrids in the crossing of Brassica pekinensis ♀$\times$Raphanus sativus ♂ were obtained, and among the three kinds of plants, Brassica, Raphanus, and F1, comparison on the mega- and microsporogenesis, megagammetophyte formation, and morphological changes in the developing sporangia, etc., were made. Differences between Brassica and Raphanus were observed in the shape of mega- and microsporangia, their changes in development, and the formation of megagametophyte. Sporangia of the F1 plants, until pre-meiotic stage, show intermediate, maternal, paternal, or vigorous inclination, and the difference of these characteristics when compared with those of the parent is slight. Meiotic irregularity resulted from the intergeneric hybridity of F1 plant gives rise to the abnormal mega- and microspore and accompanied abortive female and male gametophytes, bringing about the remarkable differences from its parent in the morphological changes of the developing mega- and microsporangia.

• 생명과학회지
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• 제7권4호
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• pp.276-281
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• 1997

This study was investigated to determine whether nicotine causes the morphological changes and expression of heat shock protein(HSP) 70 in the central nervous system of rat embryo. The pregnant rats were injected s.c. twice daily with 3 mg nicotine per 100g body weight from day 0 to 14 of gestation and embryos were removed on gestation day 15. As morphological changes, retardation of cell proliferaton was observed in the telencephalon of nicotine-treated groups and no changes in the other region were found. Minimal HSP 70 was expressed over chole central nervous system was similar between control and nicotine-treated group, the expression of blood cells in the meinges and chroid plexus was significantly greater in nicotine-treated group than in control.

• 생물환경조절학회지
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• 제32권3호
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• pp.205-209
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• 2023

기계적 자극은 식물체의 형태적 변화를 유도하는 것으로 알려져 있다. 본 연구에서는 기계적 자극을 적용한 토마토의 육묘기 동안의 생육 변화를 확인하기 위해 수행되었다. 브러싱 처리가 기계적 자극으로 이용되었으며, 아크릴이 부착된 이송장치를 이용하여 2시간 간격으로 파종 후 10일 이후부터 처리를 진행하였다. 브러싱 처리 동안 생육 변화를 확인하기 위해 3일 간격으로 생육을 조사하였다. 초장과 엽면적은 브러싱 처리에서 대조구에 비해 감소하는 결과를 보였으며 지상부의 생체중과 건물중은 대조구와 유사한 값을 보였다. 총 근장과 지하부 표면적은 브러싱 처리에서 대조구에 비해 증가하였으며, 지하부 부피는 대조구와 브러싱 처리가 유사한 값을 보였다. 결론적으로 토마토 공정묘에 브러싱 처리는 지상부의 생육 감소를 유도하였으며 지하부의 형태적 발달을 증가시키는 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1077-1086
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• 2001

The sprA and sprB gene encoding chymotrypsin-like proteases Streptomyces griseus protease A (SGPA) and Streptomyces griseus protease B (SGPB) and the sprT gene that encodes Streptomyces griseus trypsin (SGT) were cloned from Streptomyces griseus ATCC10137 and overexpressed in Streptomyces lividans TK24 as a heterologous host. The chymotrypsin activity of tole culture broth measured with the artificial chromogenic substrate , N-succinyl-ala-ala-pro-phe-p-nitroanilide, was 10, 14 and 14 units/mg in the transformants haboring the sprA, sprB and sprD genes, respectively. The growth of S. lividans reached the maximum cell mass after 4 days of culture, yet SGPA and SGPD production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The trypsin activity of the culture broth measured with the artificial chromogenic substrate , N-${\alpha}$-benzoyl-DL- arginine-p-nitroanilide , was 16 units/mg and SGT production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The introduction of the sprA gene into S, lividans TK24 triggered the biosynthesis of pigmented antibiotics, actinorhodin and undecylprodigiosin, and induced significant morphological changes in the colonies in Benedict, R2YE, and R1R2 media. In addition, the introduction of the sprT gene also induced morphological changes in the colony shape without affecting the antibiotic production, thereby implying that certain proteases would appear to play very important and specific roles in secondary-metabolites formation and morphological differentiation in Streptomyces.

• 한국해양생명과학회지
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• 제6권1호
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• pp.23-30
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• 2021

북방전복 Haliotis discus hannai 웅성생식세포의 분화과정과 정자의 형태를 미세구조적으로 기재하였다. 정자의 분화과정은 정원세포기, 정모세포기, 정세포기 및 정자기의 4단계로 구분하였다. 정원세포기에서 정모세포기로의 분화과정은 형태학적 변화가 크지 않았다. 그러나 정자변태과정 동안 염색질 응축, 핵의 형태 변화, 첨체와 중편 및 편모 형성 등의 급격한 형태학적 변화를 나타냈다. 북방전복의 정자는 두부, 중편 및 미부로 구성되며, 두부의 길이는 약 5.3 ㎛로 전자밀도가 높은 핵과 총알형의 첨체로 이루어져 있었다. 중편은 기저체와 미토콘드리아로 구성되어 있었으며, 기저체를 중심으로 5개의 미토콘드리아가 한 층으로 배열되어 있었다. 미부의 횡단면은 "9+2"의 미세소관 구조를 보였다. 이러한 형태 및 구조적 특징은 북방전복의 정자는 원시형(primitive type) 정자임을 보여주는 결과이다.

• Journal of Electrochemical Science and Technology
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• 제2권3호
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• pp.180-185
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• 2011

The layered lithium-manganese oxide ($Li_2MnO_3$) as a cathode material of lithium ion secondary batteries was prepared and characterized the physico-chemical and electrochemical properties. The morphological and structural changes of MnO(OH) and $Li_2MnO_3$ are closely connected to the changes of electrochemical properties. The crystallinity of $Li_2MnO_3$ is enhanced as the annealing temperature increase, but its capacity is reduced due to the easier structural changes of less crystalline $Li_2MnO_3$ than highly crystalline one. Moreover, the addition of buffer material such as MnO(OH) into cathode causes to reduce the morphological and structural changes of layered $Li_2MnO_3$ and increase the discharge capacity and cycleability.

• 한국동물학회:뉴스레터
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• 제12권2호
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• pp.119.2-119
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• 1995

No Abstract, See Full Text