Journal of Marine Life Science (한국해양생명과학회지)

The Korean Society of Marine Life Science (한국해양생명과학회)
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Microstructural Differentiation of Sperm in the Abalone Haliotis discus hannai (Gastropoda: Haliotidae)

북방전복 Haliotis discus hannai 정자의 미세구조적 분화

  • Kim, Hyejin (Fisheries Seed and Breeding Research Institute, National Institute of Fisheries Science) ;
  • Kim, Hyeon Jin (Department of Aqualife Medicine, Chonnam National University) ;
  • Shin, So Ryung (Department of Aqualife Medicine, Chonnam National University) ;
  • Baek, Eun Ran (Department of Aqualife Medicine, Chonnam National University) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
  • 김혜진 (국립수산과학원 수산종자육종연구소) ;
  • 김현진 (전남대학교 수산생명의학과) ;
  • 신소령 (전남대학교 수산생명의학과) ;
  • 백은란 (전남대학교 수산생명의학과) ;
  • 이정식 (전남대학교 수산생명의학과)
  • Received : 2021.01.04
  • Accepted : 2021.04.13
  • Published : 2021.05.31
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Abstract

The differentiation process of male germ cells and sperm morphology of the abalone Haliotis discus hannai were described in ultrastructure. The differentiation process of sperm was divided into four stages: spermatogonium, spermatocyte, spermatid and sperm. The process of differentiation from spermatogonium to spermatocyte did not show significant morphological changes. However, during the spermiogenesis there were distinct morphological changes such as chromatin condensation, morphological changes of the nucleus, and formation of acrosome, midpiece and flagellum. The sperm of the abalone consisted of head, midpiece and tail. The head of approximately 5.3 ㎛ in length was composed of a nucleus of high electron dense and bullet-shaped acrosome. The midpiece was composed of the basal body and mitochondria, and five mitochondria were arranged in single layer around the basal body. The cross section of the tail showed a "9+2" axonemal structure. These morphological and structural features are the result of showing that the sperm of H. discus hannai is a primitive type.

북방전복 Haliotis discus hannai 웅성생식세포의 분화과정과 정자의 형태를 미세구조적으로 기재하였다. 정자의 분화과정은 정원세포기, 정모세포기, 정세포기 및 정자기의 4단계로 구분하였다. 정원세포기에서 정모세포기로의 분화과정은 형태학적 변화가 크지 않았다. 그러나 정자변태과정 동안 염색질 응축, 핵의 형태 변화, 첨체와 중편 및 편모 형성 등의 급격한 형태학적 변화를 나타냈다. 북방전복의 정자는 두부, 중편 및 미부로 구성되며, 두부의 길이는 약 5.3 ㎛로 전자밀도가 높은 핵과 총알형의 첨체로 이루어져 있었다. 중편은 기저체와 미토콘드리아로 구성되어 있었으며, 기저체를 중심으로 5개의 미토콘드리아가 한 층으로 배열되어 있었다. 미부의 횡단면은 "9+2"의 미세소관 구조를 보였다. 이러한 형태 및 구조적 특징은 북방전복의 정자는 원시형(primitive type) 정자임을 보여주는 결과이다.

Keywords

Acknowledgement

본 연구는 해양수산부의 재원으로 농림수산식품기술기획평가원의 GoldenSeed 프로젝트 사업의 지원을 받아 연구됨(213008-05-4-SB720).

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