• Title/Summary/Keyword: morphological analysis

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Morphological and Genetic Variation of Two Populations of Platichthys stellatus (Pleuronectidae, PISCES) from the East Sea (동해 강도다리(Platichthys stellatus) 2개체군의 형태 및 분자변이)

  • Jeong, Yong Tae;Baek, Hea Ja;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.1
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    • pp.52-58
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    • 2014
  • Morphological and genetic variation of two populations of Platichthys stellatus were investigated based on 30 individuals each, collected from Uljin (seedling release area) and Pohang (control) in Korea. Morphological analyses demonstrated that the two populations of P. stellatus were well distinguishable in body color of the blind side and fin shape. Mitochondrial DNA control region analysis indicated no significant differences between the two populations ($F_{ST}=-0.00849$, P>0.05). We also analyzed microsatellite DNA loci of the two populations using six markers. Observed heterozygosity ($H_O$) and expected heterozygosity ($H_E$) were 0.550 and 0.592, respectively, in P. stellatus from Uljin, but 0.700 and 0.737 in P. stellatus from Pohang. An index of differentiation in genetic structure revealed significant differences between the two populations ($F_{ST}=0.0208$, P<0.05). Our results suggest that the Uljin population may be comprised of released P. stellatus, whereas the Pohang population may be wild P. stellatus, highlighting the necessity of continuous monitoring of the two populations.

Morphological Features of Pollen Grains in Portulaca

  • Kim, InSun
    • Applied Microscopy
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    • v.43 no.2
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    • pp.75-80
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    • 2013
  • The morphological features of pollen grains collected from the broad-leaved and cylindrical-leaved Portulaca species of the Hawaiian Islands were investigated. The variation in size and surface pattern were examined using scanning electron microscopy and statistical analysis. Pollens of the Portulaca were apolar grains of monads exhibiting apertures, colpi, and relatively thin echini. Of particular interest was the differing size of pollen grains among the species studied. Pollens of the broad-leaved Portulaca were considerable in size with a length of about $73{\sim}86{\mu}m$, while the cylindrical-leaved Portulaca tended to have smaller pollen grains ranging from 50~65 in diameter. The smallest pollens were found in cultivar 2 having an average of $43{\mu}m$. The pollen was intectate, and the exine surface appeared granulous, having sculptured elements of spinules and puncta. Two types of puncta were discerned; one forming an operculum, and the other, a simple perforation. Numerous spinules and small puncta were found throughout the cylindrical-leaved Portulaca. In the present study, morphological features of pollen grains from ten Hawaiian Portulaca species, including endemic and unknown taxa, revealed the aforementioned pattern of variation. This paper aim to provide morphological information that could be of phylogenetic value within the Hawaiian Portulaca.

Analysis of Reconstituted Tobacco Products by Characterizing Morphological Properties of Major Structure Materials (국내외산 판상엽 구성물질의 형태적 특성 비교)

  • Sung Yong-Joo;Han Young-Lim;Kim Sam-Gon;Kim Geun-Su;Joo Jeon-Hyun;Song Tae-Won
    • Journal of the Korean Society of Tobacco Science
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    • v.27 no.2
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    • pp.189-194
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    • 2005
  • The morphological properties of various structure materials of domestic and foreign reconstituted tobacco products(RTP) were investigated by using the Bauer-McNett classifier and the image analyzer. The results of the fiber classification showed the fraction of the bigger size structure materials was larger in a domestic RTP than that in two foreign RTPs. In case of fine fraction, the domestic RTP had bigger fine fraction than two foreign RTPs. Images of each structure materials showed the scrap in the foreign RTPs kept the original shape which were rare in the domestic RTP fractions. Those results deduced that the raw materials in a foreign RTP process might be treated separately depending on the mechanical and morphological properties, which could reduce the amount of fine generation and increase the efficiency in raw material treatment.

Molecular Identification of Arbuscular Mycorrhizal Fungal Spores Collected in Korea

  • Lee, Jai-Koo;Park, Sang-Hyeon;Eom, Ahn-Heum
    • Mycobiology
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    • v.34 no.1
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    • pp.7-13
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    • 2006
  • Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, AM fungi were collected from 25 sites growing with 16 host plant species in Korea and cultured with Sorghum bicolor in greenhouse condition. AM fungal spores were extracted and identified using both morphological and molecular methods. Using morphological characters, total 15 morpho-speices were identified. DNA was extracted from single spore of AM fungi and a partial region on 18S rDNA was amplified using nested PCR with AM fungal specific primers AML1/AML2. A total of 36 18S rDNA sequences were analyzed for phylogenetic analysis and 15 groups of AM fungi were identified using both morphological and molecular data of spores. Among the species, 4 species, Archaeospora leptoticha, Scutellospora castanea, S. cerradensis, S. weresubiae were described for the first time in Korea and two species in Glomus and a species in Gigaspora were not identified. Morphological and molecular identification of AM fungal spores in this study would help identify AM fungal community colonizing roots.

Intraspecific variations of the Yam (Dioscorea alata L.) based on external morphology and DNA marker analysis

  • Chang, Kwang-Jin;Yoo, Ki-Oug;Park, Cheol-Ho;Lim, Hak-Tae;Michio Onjo;Park, Byoung-Jae
    • Plant Resources
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    • v.3 no.3
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    • pp.211-218
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    • 2000
  • Intraspecific genetic relationship of 19 variation types of the Yam (Dioscorea alata) classified by their external morphological characteristics such as leaf and tuber shape were assessed by DNA using random and specific primer. Twenty two out of 113 primers (100 random[10-mer] primers, two 15 mer [M13 core sequence, and (GGAT)$_4$ sequence]) had been used in PCR-amplification. Only 12 primers, however, were success in DNA amplification in all of the analyzed plants, resulting in 93 randomly and specifically amplified DNA fragments. The analyzed taxa showed very high polymorphisms(69 bands, 71.0 %), allowing individual taxon to be identified based on DNA fingerprinting. Monomorphic bands among total amplified DNA bands of each primer was low under the 50%. Similarity indices between accessions were computed from PCR(polymerase chain reaction) data, and genetic relationships among intraspecific variations were closely related at the levels ranging from 0.66 to 0.90. These DNA data were not matched well with those of morphological characters since they were divided into two major groups at the similarity coefficient value of 0.70. Therefore, Grouping of species into variation types by mainly morphological charactistics was suggested unreasonable.

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Morphological study of a horsehair worm, Gordius sp. (Nematomorpha: Gordiida), passed in canine feces

  • Hong, Eui-Ju;Ha, Na-Ri;Ryu, Si-Yun;Chae, Joon-Seok;Kim, Hyeon-Cheol;Park, Jinho;Choi, Kyoung-Seong;Yu, Do-Hyeon;Park, Bae-Keun
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.271-276
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    • 2016
  • Horsehair or gordian worms (Nematomorpha) were identified with 22 genera (Gordiida) and 5 marine species (Nectonema) until now. During juvenile phase in development, they gain parasitic activity in arthropods. In this study, a gordian worm was detected in the feces of a dog living in Nonsan-si, Chungcheongnam-do, Korea. Using this worm, we evaluated the morphological characteristics by light microscopic analysis. Furthermore, the morphological classification was re-evaluated by scanning and transverse electron microscopes. The worm was determined that it is male adult having a bi-lobed tail and male gonads in cross sections. Based on the morphological characteristics including cross sections of body and areole on the cuticle, the parasite was also identified as Gordius sp. (Nematomorpha: Gordiidae).

Morphological segmentation based on edge detection-II for automatic concrete crack measurement

  • Su, Tung-Ching;Yang, Ming-Der
    • Computers and Concrete
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    • v.21 no.6
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    • pp.727-739
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    • 2018
  • Crack is the most common typical feature of concrete deterioration, so routine monitoring and health assessment become essential for identifying failures and to set up an appropriate rehabilitation strategy in order to extend the service life of concrete structures. At present, image segmentation algorithms have been applied to crack analysis based on inspection images of concrete structures. The results of crack segmentation offering crack information, including length, width, and area is helpful to assist inspectors in surface inspection of concrete structures. This study proposed an algorithm of image segmentation enhancement, named morphological segmentation based on edge detection-II (MSED-II), to concrete crack segmentation. Several concrete pavement and building surfaces were imaged as the study materials. In addition, morphological operations followed by cross-curvature evaluation (CCE), an image segmentation technique of linear patterns, were also tested to evaluate their performance in concrete crack segmentation. The result indicates that MSED-II compared to CCE can lead to better quality of concrete crack segmentation. The least area, length, and width measurement errors of the concrete cracks are 5.68%, 0.23%, and 0.00%, respectively, that proves MSED-II effective for automatic measurement of concrete cracks.

A New Species of Arca L., 1758 (Bivalvia: Arcidae) from New Caledonia, with Comments on the Genus

  • Lutaenko, Konstantin A.;Maestrati, Philippe
    • The Korean Journal of Malacology
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    • v.23 no.2
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    • pp.155-164
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    • 2007
  • A new species, Arca koumaci Lutaenko et Maestrati n. sp. (Bivalvia: Arcidae), is described from New Caledonia. The species is characterized by the small size, the convex shell with a strong posterior umbonal ridge covered by spikes, the widely curved ventral margin, and presence of cancellate sculpture and convergent marginal teeth. Presence of spikes on the posterior ridge is a unique morphological feature recorded for the first time in the genus. It is proposed that the only subgenus, namely Pliocene A. (Arcoptera) Heilprin, 1887, apart from nominative, can be recognized in the genus. Three morphological types are distinguished within the genus based on shell shape and sculpture. Bathymetric analysis shows that representatives of Arca inhabit water depths down to 175 m, and more than half of Recent species were found below 50 m. Types of A. bouvieri P. Fischer, 1874, Arca boucardi Jousseaume, 1894, Arca avellana Lamarck, 1819, and Arca retusa Lamarck, 1819 are illustrated.

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Relationship between morphology and viscosity of the main culture broth of Cephalosporium acremonium M25

  • Kim Jong Chae;Lim Jung Soo;Kim Jung Mo;Kim Chongyoup;Kim Seung Wook
    • Korea-Australia Rheology Journal
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    • v.17 no.1
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    • pp.15-20
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    • 2005
  • In this study, the relationship between morphology and viscosity of the main culture broth of Cephalosporium acremonium M25 was investigated in a 2.5 L bioreactor. The differentiation of C. acremonium M25 showed a complex pattern during the main culture. The morphological changes of C. acremonium M25 were related to the rheological properties of the culture broth and it was well agreed with the power law model. As a result of rheology study, it was found that rheological properties of the main culture broths of C. acremonium M25 in bioreactor were closely related to morphological changes. Also, fractal dimension fairly predicted morphological and rheological changes in the main culture broth.

Morphological characteristics of the mandibular asymmetry in adult patients (하악골비대칭 환자의 형태학적 특징에 관한 연구)

  • Lee, Tae-Hee;Yoo, Eem-Hak
    • Journal of Dental Rehabilitation and Applied Science
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    • v.17 no.4
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    • pp.225-230
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    • 2001
  • Morphological characteristics of the asymmetric mandible in 135 adult male patients were investigated. Panoramic X-ray images were recorded. Age, ramus height, antegonial notch height, condylar height, coronoid process height, ramus body height and condylar neck angle were calculated and measured. Statistical analysis of unpaired two-tailed t-test and correlation coefficients was performed to find the morphologic differences between short ramus height side and the other. Condylar height and ramus body height in short ramus height side were smaller than those of the other side. Antegonial notch height, coronoid process height, condylar neck angle showed no differences. Condylar neck height was correlated with condylar neck angle.

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