• Title/Summary/Keyword: molecular survey

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Unrecorded Fungal Species Isolated from Medicinal Plant Leaves in Korea (약용식물 잎에서 분리한 국내 미기록 진균)

  • Ahn, Geum Ran;Noh, Hyeungjin;Lee, Dong Hyeung;Kim, Susan;Kim, Jun Young;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.319-328
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    • 2019
  • As an effort to explore fungal diversity, fungal survey was undertaken in 2018 in Jangheung, Korea. For the survey, medicinal plant leaves were sampled from Selaginella tamariscina, Serratula coronata ssp. insularis, Scutellaria baicalensis, Scrophularia buergeriana and subjected to fungal isolation. Four unrecorded fungal species, Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, and Phomopsis capsici were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the ITS and 28S rDNA regions.

Survey on Nucleotide Encoding Techniques and SVM Kernel Design for Human Splice Site Prediction

  • Bari, A.T.M. Golam;Reaz, Mst. Rokeya;Choi, Ho-Jin;Jeong, Byeong-Soo
    • Interdisciplinary Bio Central
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    • v.4 no.4
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    • pp.14.1-14.6
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    • 2012
  • Splice site prediction in DNA sequence is a basic search problem for finding exon/intron and intron/exon boundaries. Removing introns and then joining the exons together forms the mRNA sequence. These sequences are the input of the translation process. It is a necessary step in the central dogma of molecular biology. The main task of splice site prediction is to find out the exact GT and AG ended sequences. Then it identifies the true and false GT and AG ended sequences among those candidate sequences. In this paper, we survey research works on splice site prediction based on support vector machine (SVM). The basic difference between these research works is nucleotide encoding technique and SVM kernel selection. Some methods encode the DNA sequence in a sparse way whereas others encode in a probabilistic manner. The encoded sequences serve as input of SVM. The task of SVM is to classify them using its learning model. The accuracy of classification largely depends on the proper kernel selection for sequence data as well as a selection of kernel parameter. We observe each encoding technique and classify them according to their similarity. Then we discuss about kernel and their parameter selection. Our survey paper provides a basic understanding of encoding approaches and proper kernel selection of SVM for splice site prediction.

GALACTIC ANTICENTER CO SURVEY: I. L = $178^{\circ}$ TO $186^{\circ}$, B = $3^{\circ}.5$ TO $6^{\circ}.0$

  • LEE Y.;JUNG J. H.;CHUNG H. S.;PARK Y. S.;KIM H. R.;KIM H. G.;KIM B. G.;KIM J. S.;HAN S. T.
    • Journal of The Korean Astronomical Society
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    • v.29 no.spc1
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    • pp.173-174
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    • 1996
  • We have mapped $17 deg^2$ region toward Galactic anticenter in $^{12}CO$ J = 1 - 0 using the 3 mm SIS receiver on the 14 m telescope at Taeduk Radio Astronomy Observatory (TRAO). The region mapped in this paper is the first target of the Galactic AntiCenter CO Survey Project (GACCOS) and was selected comparing with IRAS Sky Survey Atlas (ISSA) images at 100${\mu}m$. Molecular emission of the target area is found to be very extended and is well matching with the FIR emission boundary. There are several pieces of clouds, and as some of spectra show several peaks, there seem to be several clouds overlapped in some directions. The Velocity of Local Standard of Rest ($V_{LSR}$) of the CO emission of the mapped region ranges from -20 to +10 km/s. It is also found that the two cloudlets located around I = $180^{\circ}$ have $V_{LSR}$ = -20 km/s, which is very abnormal. The peak antenna temperature of 13 K arises near the H II Region S241.

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Sub-mm variability of a YSO (EC53) in Serpens main region : JCMT Transient survey

  • Yoo, Hyunju;Lee, Jeong-Eun;Johnstone, Doug;Mairs, Steve;Herczeg, Gregory
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.39.2-39.2
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    • 2017
  • Stars form through the gravitational collapse of molecular clouds. However, the rate at which a star gains most of its mass and the physics that drives the main phase of stellar growth is still unclear. The typical luminosity of observed protostars is smaller than what expected from the Shu's inside-out collapse model, which predicts a constant mass accretion rate. The episodic accretion model has been suggested as a solution of this luminosity problem. The JCMT Transient survey is a long term monitoring program using JCMT/SCUBA-2 to detect accretion variability of protostars in the eight nearby star-forming regions. Recently, we found a rise of the 850 micron flux at a clump in the Serpens main region at the rate of ~17% relative to the mean flux over previous observations. The submm clump is associated with a class I protostar, EC53, which has been reported as a binary system with a periodic variability. In this talk, we will provide a brief overview of the JCMT Transient Survey project, present the detection of the variable source, and discuss about follow-up observations.

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Anticoagulation after pancreatic surgery with venous resection (TIGRESS): What should we do? Results from an international survey

  • Thomas B. Russell;Debora Ciprani;Somaiah Aroori
    • Annals of Hepato-Biliary-Pancreatic Surgery
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    • v.27 no.4
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    • pp.423-426
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    • 2023
  • Backgrounds/Aims: Patients who undergo pancreatic surgery with venous resection have high rates of morbidity/mortality. Also, they are high-risk for postoperative venous thromboembolism. Whether this group should be routinely anticoagulated is unknown. This study aimed to establish current anticoagulation practices. Methods: A survey (https://form.jotform.com/220242489107048) was sent out to pancreatic surgeons. Questions covered center volume, venous resection/reconstruction techniques and anticoagulation policies. Results: Sixty-five centers from 17 countries responded. Following a "side-bite" venous resection with a patch repair, 40% used an autologous vein patch, 27% used peritoneum, and 27% used a bovine patch. After formally resecting a segment of vein, 17% of centers used an interposition graft (IG). Left renal vein (41%) and polytetrafluoroethylene (73%) grafts were the most commonly used autologous and prosthetic IGs, respectively. Following a prosthetic IG, an autologous IG, and a "side-bite" resection, 59%, 28%, and 19% of centers provided therapeutic anticoagulation, respectively (66% used low molecular-weight heparin). The duration of therapy provided varied from inpatient stay only (14%) to six months (32%). Conclusions: Our global survey indicates that anticoagulation practices are highly variable. Centers do not agree on when to anticoagulate, how to anticoagulate, or the duration of therapy. A robust trial is required to provide clarity.

A MULTI-DIMENSIONAL REDUCTION METHOD OF LARGE-SCALE SURVEY DATABASE

  • Lee, Y.;Kim, Y.S.;Kang, H.W.;Jung, J.H.;Lee, C.H.;Yim, I.S.;Kim, B.G.;Kim, H.G.;Kim, K.T.
    • Publications of The Korean Astronomical Society
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    • v.28 no.1
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    • pp.7-13
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    • 2013
  • We present a multi-dimensional reduction method of the surveyed cube database obtained using a single- dish radio telescope in Taeduk Radio Astronomy Observatory (TRAO). The multibeam receiver system installed at the 14 m telescope in TRAO was not optimized at the initial stage, though it became more stabilized in the following season. We conducted a Galactic Plane survey using the multibeam receiver system. We show that the noise level of the first part of the survey was higher than expected, and a special reduction process seemed to be definitely required. Along with a brief review of classical methods, a multi-dimensional method of reduction is introduced; It is found that the 'background' task within IRAF (Image Reduction and Analysis Facility) can be applied to all three directions of the cube database. Various statistics of reduction results is tested using several IRAF tasks. The rms value of raw survey data is 0.241 K, and after primitive baseline subtraction and elimination of bad channel sections, the rms value turned out to be 0.210 K. After the one-dimensional reduction using 'background' task, the rms value is estimated to be 0.176 K. The average rms of the final reduced image is 0.137 K. Thus, the image quality is found to be improved about 43% using the new reduction method.

C13orf18 and C1orf166 (MULAN) DNA Genes Methylation are Not Associated with Cervical Cancer and Precancerous Lesions of Human Papillomavirus Genotypes in Iranian Women

  • Sohrabi, Amir;Mirab-Samiee, Siamak;Rahnamaye-Farzami, Marjan;Rafizadeh, Mitra;Akhavan, Setareh;Hashemi-Bahremani, Mohammad;Modarressi, Mohammad Hossein
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.16
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    • pp.6745-6748
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    • 2014
  • Background: Nowadays, molecular biomarkers have critical roles for cancer diagnosis and prognosis in clinical laboratories. Human papillomaviruses are the main agents for etiology of cervical carcinoma. The present survey was conducted to evaluate the genes methylation in cervical cancer and precancerous lesions involvement with HPV genotypes. Materials and Methods: C13orf18 and C10rf166 (MULl or Mulan) DNA methylation as potential biomarkers and risk factors was investigated in 112 liquid based cytology and Formalin-Fixed Paraffin-Embedded tissue specimens in Iranian females with cervical intraepithelial neoplasia and dysplasia. Results: In this survey, HPV18 (61.6%) and HPV16 (42.9%) proved to be the most common HPV genotypes identified by In-House Multiplex Real Time PCR. There were no significant relationship between HPV positivity and the methylated DNA genes mentioned above (p>0.05). Conclusions: Our MethyLight data demonstrated that these genes could not be considered as specific, sensitive and suitable prognostic biomarkers in cervical dysplasia related HPV. It is suggested that further studies with more patients should be done on candidate methylated markers in different countries in order to plan for cervical cancer prevention.

Simultaneous 22GHz Water and 44GHz Methanol Maser Survey of Ultra-compact HII Regions

  • Kim, Won-Ju;Kim, Kee-Tae;Kim, Kwang-Tae
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.83.2-83.2
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    • 2012
  • We have carried out simultaneous 22GHz $H_2O$ and 44GHz Class I $CH_3OH$ maser survey of 112 ultra-compact HII regions (UCHIIs) twice in 2010 and 2011. We detected $H_2O$ maser and $CH_3OH$ maser emission from 76(68%) and 49(44%) UCHIIs, respectively. Among them 15 $H_2O$ masers and 32 $CH_3OH$ masers are new detections. These high detection rates suggest that the occurrence periods of both masers are significantly overlapped with the UCHII phase. $CH_3OH$ masers always have small (<10 km s-1) relative velocities with respect to the natal molecular cores, while $H_2O$ masers often show larger velocities. We find 20 UCHIIs with $H_2O$ maser lines at relative velocities >30 km s-1. The formation and disappearance of $H_2O$ masers is frequent over one-year time interval. In contrast, $CH_3OH$ masers usually do not show substantial variation in intensity, velocity, or shape. The isotropic luminosities of both masers well correlate with the bolometric luminosities of the central stars when data points of lowand intermediate-mass protostars are added: $L_{H_2O}=5.89{\times}10^{-9}{(L_{bol})^{0.69}}$ and $L_{CH_3OH}=4.27{\times}10^{-9}{(L_{bol})^{0.62}}$. They also tend to increase with the 2cm radio continuum luminosity of UCHIIs and the 850 um continuum luminosity of the associated molecular cores. We discuss some individual sources.

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KVN single-dish survey of the MALATANG galaxies

  • Poojon, Panomporn;Chung, Aeree;Lee, Bumhyun;Baek, Junhyun;Jung, Taehyun;Sohn, Bong Won;Oh, Se-Heon;Sengupta, Chandreyee
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.57.2-57.2
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    • 2018
  • We present the preliminary result from our KVN single-dish observations of the MALATANG sample. The MALATANG (Mapping the dense molecular gas in the strongest star-forming Galaxies) is one of the JCMT legacy surveys on the nearest 23 IR-brightest galaxies beyond the Local Group. The goal of the MALATANG survey is to map the sample in the dense gas tracers (HCN and HCO+J=4-3), and probe the relationships between the dense molecular gas and star formation activities. As a complementary study, we recently launched a KVN/KaVA program on the same sample, in order to measure their flux densities and parsec-scale jet/outflows in the millimeter regime, which will be greatly useful in understanding the initial conditions of the feedback process. In this work, we present the preliminary result from our pilot KVN single-dish program on a sub-sample, which will be used to select the future VLBI imaging study under plan. We investigate the KVN spectral energy distributions (SED) of the sample as a function of the power source of the luminous IR brightness of each target (starburst? AGN? or hybrid?). We also discuss the technical challenges that we experienced during our KVN observations due to the large size of the sample in the sky.

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Molecular Prevalence of Acarapis Mite Infestations in Honey Bees in Korea

  • Ahn, Ah-Jin;Ahn, Kyu-Sung;Noh, Jin-Hyeong;Kim, Young-Ha;Yoo, Mi-Sun;Kang, Seung-Won;Yu, Do-Hyeon;Shin, Sung Shik
    • Parasites, Hosts and Diseases
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    • v.53 no.3
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    • pp.315-320
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    • 2015
  • Acarapis mites, including Acarapis woodi, Acarapis externus, and Acarapis dorsalis, are parasites of bees which can cause severe damage to the bee industry by destroying colonies and decreasing honey production. All 3 species are prevalent throughout many countries including UK, USA, Iran, Turkey, China, and Japan. Based on previous reports of Acarapis mites occurring in northeast Asia, including China and Japan, we investigated a survey of Acarapis mite infestations in honey bees in Korean apiaries. A total of 99 colonies of Apis mellifera were sampled from 5 provinces. The head and thorax of 20 bees from each colony were removed for DNA extraction. PCR assays were performed with 3 primer sets, including T, A, and K primers. Results indicated that 42.4% (42/99) of samples were Acarapis-positive by PCR assay which were sequenced to identify species. Each sequence showed 92.6-99.3% homology with reference sequences. Based on the homology, the number of colonies infected with A. dorsalis was 32 which showed the highest infection rate among the 3 species, while the number of colonies infected with A. externus and A. woodi was 9 and 1, respectively. However, none of the Acarapis mites were morphologically detected. This result could be explained that all apiaries in the survey used acaricides against bee mites such as Varroa destructor and Tropilaelaps clareae which also affect against Acarapis mites. Based on this study, it is highly probable that Acarapis mites as well as Varroa and Tropilaelaps could be prevalent in Korean apiaries.