Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Molecular Prevalence of Acarapis Mite Infestations in Honey Bees in Korea

  • Ahn, Ah-Jin (Department of Parasitology, College of Veterinary Medicine, Chonnam National University) ;
  • Ahn, Kyu-Sung (Department of Parasitology, College of Veterinary Medicine, Chonnam National University) ;
  • Noh, Jin-Hyeong (Animal and Plant Quarantine Agency (QIA)) ;
  • Kim, Young-Ha (Animal and Plant Quarantine Agency (QIA)) ;
  • Yoo, Mi-Sun (Animal and Plant Quarantine Agency (QIA)) ;
  • Kang, Seung-Won (Animal and Plant Quarantine Agency (QIA)) ;
  • Yu, Do-Hyeon (Department of Clinical Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Sung Shik (Department of Parasitology, College of Veterinary Medicine, Chonnam National University)
  • Received : 2015.01.04
  • Accepted : 2015.05.30
  • Published : 2015.06.30
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Abstract

Acarapis mites, including Acarapis woodi, Acarapis externus, and Acarapis dorsalis, are parasites of bees which can cause severe damage to the bee industry by destroying colonies and decreasing honey production. All 3 species are prevalent throughout many countries including UK, USA, Iran, Turkey, China, and Japan. Based on previous reports of Acarapis mites occurring in northeast Asia, including China and Japan, we investigated a survey of Acarapis mite infestations in honey bees in Korean apiaries. A total of 99 colonies of Apis mellifera were sampled from 5 provinces. The head and thorax of 20 bees from each colony were removed for DNA extraction. PCR assays were performed with 3 primer sets, including T, A, and K primers. Results indicated that 42.4% (42/99) of samples were Acarapis-positive by PCR assay which were sequenced to identify species. Each sequence showed 92.6-99.3% homology with reference sequences. Based on the homology, the number of colonies infected with A. dorsalis was 32 which showed the highest infection rate among the 3 species, while the number of colonies infected with A. externus and A. woodi was 9 and 1, respectively. However, none of the Acarapis mites were morphologically detected. This result could be explained that all apiaries in the survey used acaricides against bee mites such as Varroa destructor and Tropilaelaps clareae which also affect against Acarapis mites. Based on this study, it is highly probable that Acarapis mites as well as Varroa and Tropilaelaps could be prevalent in Korean apiaries.

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References

  1. Jong DD, Morse RA, Eickwort GC. Mite pests of honey bees. Annu Rev Entomol 1982; 27: 229-252. https://doi.org/10.1146/annurev.en.27.010182.001305
  2. Delfinado-Baker M, Baker EW. Notes on honey bee mites of the genus Acarapis Hirst (Acari: Tarsonemidae). Int J Acarol 1982; 8: 211-226. https://doi.org/10.1080/01647958208683299
  3. Sammataro D, Gerson U, Needham G. Parasitic mites of honey bees: life history, implications, and impact. Annu Rev Entomol 2000; 45: 519-548. https://doi.org/10.1146/annurev.ento.45.1.519
  4. Eischen FA, Cardoso-Tamez D, Wilson WT, Dietz A. Honey production of honey bee colonies infested with Acarapis woodi (Rennie). Apidologie 1989; 20: 1-8. https://doi.org/10.1051/apido:19890101
  5. Kojima Y, Toki T, Morimoto T, Yoshiyama M, Kimura K, Kadowaki T. Infestation of Japanese native honey bees by tracheal mite and virus from non-native European honey bees in Japan. Microb Ecol 2011; 62: 895-906. https://doi.org/10.1007/s00248-011-9947-z
  6. Cakmak I, Aydin L, Gulegen E, Wells H. Varroa (Varroa destructor) and tracheal mite (Acarapis woodi) incidence in the Republic of Turkey. J Apicult Res 2003; 42: 57-60. https://doi.org/10.1080/00218839.2003.11101093
  7. Yang B, Peng G, Li T, Kadowaki T. Molecular and phylogenetic characterization of honey bee viruses, Nosema microsporidia, protozoan parasites, and parasitic mites in China. Ecol Evol 2013; 3: 298-311. https://doi.org/10.1002/ece3.464
  8. Jung JK, Lee MY, Mah YI. Infestation of Varroa jacobsoni and Tropilaelaps clareae in some apiaries during spring and fall seasons, 1999-2000 in South Korea. Korean J Apicult 2000; 15: 141-145.
  9. Garrido-Bailon E, Bartolome C, Prieto L, Botías C, Martínez-Salvador A, Meana A, Martín-Hernandez R, Higes M. The prevalence of Acarapis woodi in Spanish honey bee (Apis mellifera) colonies. Exp Parasitol 2012; 132: 530-536. https://doi.org/10.1016/j.exppara.2012.08.018
  10. Kojima Y, Yoshiyama M, Kimura K, Kadowaki T. PCR-based detection of a tracheal mite of the honey bee Acarapis woodi. J Invertebr Pathol 2011; 108: 135-137.
  11. Clinch PG, Faulke J. Toxicitv to the external mite Acarapis externus Morgenthaler of pesticides fed in sugar syrup to infested honev bees. New Zeal J Exp Agr 1977; 5: 185-187. https://doi.org/10.1080/03015521.1977.10425962
  12. Lorenzen K, Gary NE. Modified dissection technique for diagnosis of tracheal mites (Acari: Tarsonemidae) in honey bees (Hymenoptera: Apidae). J Econ Entomol 1986; 79: 1401-1403. https://doi.org/10.1093/jee/79.5.1401
  13. Choi YS, Lee ML, Lee MY, Lee KG. Occurrence of seven honeybee viruses and research of disease occurrence in Korean apiaries. Korean J Apicult 2008; 23: 153-159.
  14. Sammataro D, De Guzman L, George S, Ochoa R, Otis GW. Standard methods for tracheal mite research. J Apicultl Res 2013; 52
  15. Shaw FR, Fischang WJ, Balboni ER, Eversole JW. External mites on honey bees in the US. Glean Bee Cult 1961; 89: 402-403, 447.
  16. Eckert JE. Acarapis mites of the honey bee, Apis mellifera Linnaeus. J Insect Pathol 1961; 3: 409-425.
  17. Otis GW, Bath JB, Randall DL, Grant GM. Studies of the honey bee tracheal mite (Acarapis woodi) (Acari: Tarsonemidae) during winter. Can J Zool 1988; 66: 2122-2127. https://doi.org/10.1139/z88-315

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