• 생명과학회지
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• 제25권6호
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• pp.646-655
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• 2015

벽방산의 산림식생에 대한 식물사회학적 연구를 수행하였다. 연구결과 벽방산은 3개 상관형의 9개 단위식생이 분포하고 있는 것으로 확인되었다. 연구지역 단위식생은 상록침엽수림의 해송-사스레피나무군락, 소나무-자금우군락, 하록활엽수림의 졸참나무-홀아비꽃대군락, 신갈나무-우산나물군락, 상수리나무군락, 소사나무군락, 굴참나무-쇠물푸레나무군락, 비목-물참대군락, 인공조림식생의 사방오리나무식재림 등으로 구분되었다. 벽방산은 지역 최고봉임에도 불구하고 산지 전역이 숲 가꾸기사업, 등산객, 조림 및 육림 등에 의해 직/간접적으로 인위적 간섭을 받고 있는 것으로 확인되었다. 연구지역 내 식생형 가운데 상대적으로 자연성이 높은 단위식생은 산지대 사면 중부에서 출현하는 졸참나무-홀아비꽃대군락, 전석지에서 확인된 비목-물참대군락 등이며, 산지능선부의 암석노출지를 중심으로 위극상의 천이계열에 해당하는 특산식물군락인 소사나무군락이 분포하고 있었다. CCA분석 결과, 각 단위식생의 발달은 해발고도, 인간간섭, 낙엽부식층, 암석노출율, 경사도 등에 영향을 받는 것으로 확인되었다. 단위식생의 종조성에 대한 집괴분석 결과로부터 벽방산 산림식생은 인위식생, 이차식생, 근자연식생으로 구분되며, 식생의 발달정도, 토지적 특성, 인위적 식재 및 관리 유무 등에 의해 삼림이 구성되고 있는 것으로 확인되었다.

• 한국자원식물학회지
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• 제27권6호
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• pp.687-700
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• 2014

Rice blast (Magnaporthe oryza B.) is one of the most important diseases in rice that causing great yield losses every year around the world. It is important to screen valuable genetic resources for improving blast resistance. This study was conducted to identify the blast resistance in 279 Korean rice landraces using blast nursery tests and isolate inoculum screening. The results showed that 11 landrace accessions found to be resistant to rice blast in blast nursery and inoculation screening tests and the degree of lesions in most accessions showed that they were susceptible to reactions. In order to find the distribution of blast resistant genes, a molecular survey was conducted to identify the presence of major blast resistance (R) gene in 279 Korean landraces. The results revealed that their frequency distribution was Pik-m (36.2%), Piz (25.4%), Pit (13.6%), and Pik (10%). Besides, the frequency distribution of Piz-t, Pii, Pik-m/Pik-p, Pi-39(t), Pib, Pi-d(t)2, Pita/Pita-2 and Pi-ta genes were identified as less than 10%. The results did not consist with the reactions against blast diseases between genotypes and phenotypic part of the nursery tests and isolate inoculation. For concluding these results, we used genome-wide SSR markers that have closely been located with resistance genes. The PCoA analysis showed that the landrace accessions formed largely two distinct groups according to their degree of blast resistance. By comparing genetic diversities using polymorphic information contents (PIC) value among the resistant, total and susceptible landraces, we found that PIC values decreased in four SSR markers and increased in six markers in the resistant accessions, which showed contrary to total and susceptible groups. These regions might be linked to resistance alleles. In this study, we evaluated the degree of blast resistance and the information about the distribution of rice blast resistant genes in Korean rice landraces. This study might be the basis for association analysis of blast resistance in rice.

• 환경생물
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• 제36권3호
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• pp.352-358
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• 2018

본 연구는 PCR 기반 RFLP (Restriction Fragment Length Polymorphism; 제한절편 길이 다형성) 분자기법을 활용하여 난 및 치어 대상 납자루아과 어류 3종의 동정을 좀 더 빠르고 정확하게 파악하고 납자루아과 어류의 종별 산란양상 및 번식생태 이해에 대한 기여가 목적이다. 본 연구를 위해 기존 선행된 문헌자료를 확인하고 납자루아과 어류가 2종 이상 동서하고 있는 지역을 확인하여 현지조사를 수행하였다. 현지조사 결과 확인된 납자루아과 어류는 묵납자루(Acheilognathus signifer), 줄납자루(A. yamatsutae) 및 각시붕어(Rhodeus uyekii)로 총 3종이 확인되었으며, 확인된 납자루아과 어류와 동서하고 있는 숙주조개(작은말조개; Unio douglasiae sinuolatus)를 채집하여 숙주조개 속 납자루아과 어류의 난 및 치어를 확보하였다. 현지조사 결과 확인된 납자루아과 어류 3종을 대상으로 미토콘드리아 DNA COI과 cyt b 유전자 염기서열을 비교하여 각각 종별로 특이성을 지닌 부위(단일염기변이; Single Nucleotide Variation: SNV)에 맞는 제한효소를 선정하였고, 숙주조개 속 난 및 치어를 대상으로 genomic DNA를 추출하여 PCR-RFLP 실험을 수행한 결과 현지조사 시 확인된 납자루아과 어류 3종의 독특한 제한절편 길이 양상을 전기영동을 통하여 확인하였다. 본 연구를 통해 묵납자루, 줄납자루 및 각시붕어의 종을 판별할 수 있는 RFLP 마커를 개발하였으며, 숙주조개 난 및 치어를 대상으로 정확한 종의 동정을 보다 빠르고 효과적으로 수행하여 각각 납자루아과 종별 산란양상을 보다 정확히 규명하고 향후 이들 자연개체군의 효과적인 유지, 관리 및 보전 방법 개발에 유용하게 활용될 수 있을 것으로 판단된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제27권2호
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• pp.50-60
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• 2022

The concentration levels and distribution characteristics of 16 polycyclic aromatic hydrocarbons (PAHs) were investigated and evaluated for total 100 soil samples as a part of the survey on soil contamination in Gwangju. The results (median and range) of T-PAHs (sum of 16 PAH concentrations), C-PAHs (sum of carcinogenic PAH concentrations) and T-TEQs (sum of 16 TEQ concentrations) were 20.8 (7.6~1158.1), 2.2 (N.D~509.6), and 0.3 (N.D~424.6) ㎍/kg, respectively. There was a positive correlation between C-PAHs/T-PAHs and T-TEQs/T-PAHs except one point where the concentration of benzo(a)pyrene was high. The ratios of the C-PAHs/T-PAHs were 31.7% for low molecular weight-PAHs and 68.3% for high molecular weight-PAHs, suggesting that PAHs generation mainly arose from combustion sources. The ratio of isomers of individual PAHs, Phe/Ant, Flu/Pyr, Ant/(Ant+Phe), Flu/(Flu+Pyr), and BaA/(BaA+Chr), also confirmed the predominance of PAHs from combustion activities. Statistical tracing of the source of PAHs through principal component analysis indicated that the main sources of combustion were automobile fuel and coal. The overall results of this study suggested HMW-PAHs, T-PAHs, C-PAHs and T-TEQs should be separately evaluated to better assess the toxicity and environmental behavior of individual PAHs.

• 한국환경복원기술학회지
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• 제23권2호
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• pp.69-89
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• 2020

Scientific trust and quantification of traditional species investigation and results that have been used in ecology for decades has always been a problem and concern for ecologists. Global ecologists have proposed DNA-based species investigation studies to find answers to problems. In this study, we reviewed the global trend of research on environmental DNA(eDNA), which is a method for monitoring species by detecting DNA of organisms naturally mixed in environmental samples such as water, soil, and feces. The first eDNA research confirmed the possibility of species investigation at the molecular level, and commercialization of NGS(Next Generation Sequencing) and DNA metabarcoding elicits efficient and quantitative species investigation results, and eDNA research is increasing in the filed of ecology. In this study, mammals and birds were detected using MiMammal universal primers from 23 samples(3 natural reserves; 20 water bowls) out of 4 patches to verify eDNA for urban ecosystems in Suwon, and eDNA was verified by performing camera trapping and field survey. Most terrestrial species were detected through eDNA, and particularly, mice(Mus musculus), and Vinous-throated Parrotbill (Sinosuthora webbiana) were identified only with eDNA, It has been confirmed to be highly effective by investigating techniques for small and internal species. However, due to the lack of resolution of the primer, weasels(Mustela sibirica) and squirrels(Melanochromis auratus) were not detected, and it was confirmed that the traditional investigation method was effective only for a few species, such as Mogera robusta(Mogera robusta). Therefore, it is judged that the effects of species investigation can be maximized only when eDNA is combined with traditional field survey and Camera trapping to complement each other.

• The Plant Pathology Journal
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• 제33권1호
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• pp.43-52
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• 2017

A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1-97.6%) and the New Zealand isolate-U31286 (95.8-97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that Trifolium alexandrinum, Nicotiana occidentalis, Chenopodium glaucum, and Lathyrus sativus are new host plant species for LTSV and that N. occidentalis being a good propagative host for it.

• 한국버섯학회지
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• 제8권4호
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• pp.157-160
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• 2010

신령버섯은 항암작용과 더불어 면역강화작용 등 약용으로서 이용되고 있습니다. 최근에는 신령버섯 재배농가에서 새로운 병징 현상이 보고되어 내생세균에 대한 분자생물학적 방법으로 조사하였다. 병징을 나타내는 수집된 기형버섯을 eubacterial 16S rDNA 영역을 이용하여 PCR한 결과 기형버섯만 증폭되었다. 이 영역을 부분적으로 염기서열을 결정한 결과 CFB bacterium과 유사성이 가장 높았고, 이 염기서열을 이용하여 프라이머를 디자인한 후 nested PCR를 부위별로 확인한 결과 기형을 일으킨 갓의 주름살 부위에서 가장 강하게 증폭되었고 포자수확도 되지 않았으며 배양불능세균 group인 CFB bacterium임을 확인하였다.

• 패션비즈니스
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• 제14권3호
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• pp.128-145
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• 2010

Nowadays people are turning to have a more intimate environment-friendly lifestyles. In the clothing industry they are focusing on making products that are more human-friendly and not harmful to the environment. In the midst of the spread of consumption research is being done to develop manufacturing textiles that do not induce pollution. For instance, advantages of the natural textile fibers, cotton, silk, and ramie, have been newly recognized in terms of environment-friendliness, Together with these fibers, the natural high molecular materials, such as chitosan and hyaluronic acid, have found new roles in the application sectors of human-friendliness and environment-friendliness. Products using these substances and processing methods can make the products more wearable, have high sensitivity, make people feel aesthetic appreciation for the products, and make them appreciate the value of a more healthier environment. In a survey according to subjects in their 20s and 30s, their preferences towards their consciousness and awareness of the development of materials as well as their attitude towards environment-friendly products were determined as a conclusion. It was shown that consumers that are more conscious about the interest of the environment as well as the problems concerning the environment were more intent on buying products that were environmentally-friendly. Women have shown more active and positive attitudes towards the importance of the awareness of the environment in comparison with men. Generally, consumers in their 20s and 30s preferred the feel of materials that were less than 1% concentration process than those of materials that were 1% concentration process. To increase the "rustly" feeling of the materials, it was found that 1% concentration process was suitable for manufacturing.

• The Plant Pathology Journal
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• 제22권1호
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• pp.97-102
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• 2006

A virus causing vein banding, sometimes yellow mosaic and rugose symptoms on peanut was prevalent around Suwon, Korea. A survey conducted in the area found disease incidence, depending on cultivar, to range from 79 to $100\%$. The virus was found to be seed-transmissible in all the five peanut cultivars tested with transmission rates ranging from 2 to $16\%$. Host range analysis failed to differentiate 9 field isolates collected from different peanuts cultivars showing various symptoms. Inclusion bodies such as scroll, pinwheel and long laminated aggregates induced by the virus in host plant cells were similar to those induced by members of the Potyvirus subdivision III. The virus showed < $95\%$ homology with Bean common mosaic virus (BCMV), BCMV-BICMV/AzMV strains and only < $91\%$ with Desmodium mosaic virus. Based on biological characterization, electron microscopy and molecular analyses of a Korean isolate (Daewon 1), the virus was identified as peanut stripe strain of BCMV.

• Parasites, Hosts and Diseases
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• 제56권2호
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• pp.121-127
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• 2018

To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately $0.21-1.25{\mu}m$ in length and $0.05-0.07{\mu}m$ in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.