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http://dx.doi.org/10.7732/kjpr.2014.27.6.687

Blast Resistant Genes Distribution and Resistance Reaction to Blast in Korean Landraces of Rice (Oryza sativa L.)  

Song, Jae Young (National Agrobiodiversity Center, NAAS, RDA)
Lee, Gi-An (National Agrobiodiversity Center, NAAS, RDA)
Choi, Yu-Mi (National Agrobiodiversity Center, NAAS, RDA)
Lee, Sukyeung (National Agrobiodiversity Center, NAAS, RDA)
Lee, Kwang Beom (National Agrobiodiversity Center, NAAS, RDA)
Bae, Chang-Hyu (Deparment of Bioresources Science, Sunchon National University)
Jung, Yeonju (National Agrobiodiversity Center, NAAS, RDA)
Hyun, Do-Yoon (National Agrobiodiversity Center, NAAS, RDA)
Park, Hong-Jae (National Agrobiodiversity Center, NAAS, RDA)
Lee, Myung-Chul (National Agrobiodiversity Center, NAAS, RDA)
Publication Information
Korean Journal of Plant Resources / v.27, no.6, 2014 , pp. 687-700 More about this Journal
Abstract
Rice blast (Magnaporthe oryza B.) is one of the most important diseases in rice that causing great yield losses every year around the world. It is important to screen valuable genetic resources for improving blast resistance. This study was conducted to identify the blast resistance in 279 Korean rice landraces using blast nursery tests and isolate inoculum screening. The results showed that 11 landrace accessions found to be resistant to rice blast in blast nursery and inoculation screening tests and the degree of lesions in most accessions showed that they were susceptible to reactions. In order to find the distribution of blast resistant genes, a molecular survey was conducted to identify the presence of major blast resistance (R) gene in 279 Korean landraces. The results revealed that their frequency distribution was Pik-m (36.2%), Piz (25.4%), Pit (13.6%), and Pik (10%). Besides, the frequency distribution of Piz-t, Pii, Pik-m/Pik-p, Pi-39(t), Pib, Pi-d(t)2, Pita/Pita-2 and Pi-ta genes were identified as less than 10%. The results did not consist with the reactions against blast diseases between genotypes and phenotypic part of the nursery tests and isolate inoculation. For concluding these results, we used genome-wide SSR markers that have closely been located with resistance genes. The PCoA analysis showed that the landrace accessions formed largely two distinct groups according to their degree of blast resistance. By comparing genetic diversities using polymorphic information contents (PIC) value among the resistant, total and susceptible landraces, we found that PIC values decreased in four SSR markers and increased in six markers in the resistant accessions, which showed contrary to total and susceptible groups. These regions might be linked to resistance alleles. In this study, we evaluated the degree of blast resistance and the information about the distribution of rice blast resistant genes in Korean rice landraces. This study might be the basis for association analysis of blast resistance in rice.
Keywords
Rice landraces; Blast; M. grisea; Molecular marker;
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1 Chen, X.J., D. Shang, C. Chen, Y. Lei, Y. Zou , W. Zhai , G. Liu, J. Xu, Z. Ling, G. Cao, B. Ma, Y. Wang, X. Zhao, S. Li and L. Zhu. 2006. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J. 46:794-804.   DOI   ScienceOn
2 Ahn, S.N., Y.K. Kim, H.C. Hong, S.S. Han, S.J. Kwon, H.C. Choi, H.P. Moon and S.R. McCouch. 2000. Molecular mapping of a new gene for resistance to rice blast. Euphytica 116:17-22.   DOI   ScienceOn
3 Ballini, E., J.B. Morel, G. Droc, A. Price, B. Courtois, J.L. Notteghem and D. Tharreau. 2008. A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance. Mol. Plant Microbe Interact. 21:859-868.   DOI   ScienceOn
4 Bonman, J.M., T.I. Vergel de Dios and M.M. Khin. 1986. Physiologic specialization of Pyricularia oryzae in the Philippines. Plant Disease 70:767-769.   DOI
5 Chen, S., L. Wang, Z.Q. Que, R.Q. Pan and Q.H. Pan. 2005. Genetic and physical mapping of Pi37(t), a new gene conferring resistance to rice blast in the famous cultivar St. No. 1. Theor. Appl. Genet. 111:1563-1570.   DOI   ScienceOn
6 Chen, X.W., S.G. Li and J.C. Xu. 2004. Identification of two blast resistance genes in a rice variety, Digu. J. Phytopathol. 152:77-85.   DOI
7 Cho, Y.C., I.S. Choi, S.S. Han, Y.S. Shin, H.P. Moon and H.S. Suh. 1996. Inheritance of resistance to blast (Pyricularia grisea Sacc.) in Korean weedy rice (Oryza sativa L.). Korean J. Breed. Sci. 28(3):309-316.
8 Goh, J., B.R. Kim, S.W. Lee, J.H. Roh, D.B. Shin, J.U. Jeung, Y.C. Cho and S.S. Han. 2013. Selection of representative Magnaporthe oryzae isolates and rice resistant gene types for screening of blast-resistant rice cultivars. Res. Plant Dis. 19(4):243-253.   과학기술학회마을   DOI   ScienceOn
9 Han, S.S., J.D. Ryu, H.S. Shim, S.W. Lee, Y.K. Hong and K.H. Cha. 2001. Breakdown of resistant cultivars by new race KI-1117a and race distribution of rice blast fungus during 1999-2000 in Korea. Res. Plant Dis. 7:86-92 (in Korean).
10 Hittalmani, S., A. Parco, T.V. Mew, R.S. Zeigler and N. Huang. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor. Appl. Genet. 100:1121-1128.   DOI   ScienceOn
11 Harlan, J.R. 1975.Crops and Man. Madison, Wisconsin: American Society of Agronomy and Crop Science Society of America.
12 Hayashi, K., N. Hashimoto, M. Daigen and I. Ashikawa. 2004. Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus. Theor. Appl. Genet. 108:1212-1220.   DOI   ScienceOn
13 Hayashi, K., H. Yoshida and I. Ashikawa. 2006. Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes. Theor. Appl. Genet. 113:251-260.   DOI   ScienceOn
14 Kump, B and B. Javornik. 1996. Evaluation of genetic variability among common buckwheat (Fagopyrum esculentum Moench) populations by RAPD markers. Plant Science 114:149-158.   DOI   ScienceOn
15 Kinoshita, C.T. 1995. Report of Committee on Gene Symbolization, Nomenclature and Linkage Group. Rice Genet. Newsl. 12:9-153.
16 Kobayashi, A., K. Ebana, S. Fukuoka and T. Nagamine. 2006. Microsatellite markers revealed the genetic diversity of an Old Japanese Rice Landrace 'Echizen'. Genet. Resour. Crop Evol. 53(3):499-506.   DOI
17 Koide, Y., N. Kobayashi, D. Xu and Y. Fukuta. 2009. Resistance genes and selection DNA markers for blast disease in rice (Oryza sativa L.). JARQ. 43(4):255-280.   DOI   ScienceOn
18 Jeon, J.S., D. Chen, G.H. Yi, G.L. Wang and P.C. Ronald. 2003. Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast. Mol. Genet. Genomics 269:280-289.
19 Jeung, J.U., B.R. Kim, Y.C. Cho, S.S. Han, H.P. Moon, Y.T. Lee and K.K. Jena. 2007. A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motif confers broad spectrum of blast resistance in rice. Theor. Appl. Genet. 115:1163-1177.   DOI   ScienceOn
20 Li, J., D. Li, Y. Sun and M. Xu. 2012. Rice blast resistance gene Pi1 Identified by MRG4766 marker in 173 Yunnan rice landraces. Rice Genomics and Genet. 3:13-18.
21 Jia, Y.L., Z.H. Wang and P. Singh. 2002. Development of dominant rice blast Pi-ta resistance gene markers. Crop Sci. 42:2145-2149.   DOI   ScienceOn
22 Jia, Y., M. Redus, Z. Wang and J.N. Rutger. 2004. Development of a SNLP marker from the Pi-ta blast resistance gene by tri-primer PCR. Euphytica 138:97-105.   DOI
23 Liu, X.Q., Q.Z. Yang, F. Lin, L.X. Hua, C.T. C. Wang, L. Wang and Q. Pan. 2007. Identification and fine mapping of Pi39(t), a major gene conferring the broad-spectrum resistance to Magnaporthe oryzae. Mol. Genet. Genomics 278:403-410.   DOI   ScienceOn
24 Lee, S.K., M.Y. Song, Y.S. H.K. Seo, Kim, S. Ko, P.J. Cao, J.P. Suh, G. Yi, J.H. Roh, S. Lee, G. An, T.R. Hahn, G.L. Wang, P. Ronald and J.S. Jeon. 2009. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiledcoil-nucleotide-binding-leucine-rich repeat genes. Genetics 181:1627-1638.   DOI   ScienceOn
25 Lin, F., S. Chen, Z. Que, L. Wang, X. Liu and Q. Pan. 2007. The blast resistance gene Pi37 encodes an NBS-LRR protein and is a member of a resistance gene cluster on rice chromosome 1. Genetics 177:1871-1880.   DOI   ScienceOn
26 Liu, K. and S.V. Muse. 2005. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128-2129.   DOI   ScienceOn
27 Maclean, J.L., D.C. Dawe, B. Hardy and G.P. Hettel. 2002. Rice almanac (Third Edition). Philippines, IRRI, WARDA, CIAT and FAO.
28 McCouch, S.R., R.J. Nelson, J. Thome and R.S. Zeigler. 1994. Mapping of blast resistance genes in rice: In Zeigler, R.S., S.A. Leong and P.S. Teng (eds.), Rice Blast Disease, Cab Int'l and IRRI, Wallingford, Oxon, UK. pp. 167-186.
29 SES, IRRI, 2002. Standard Evaluation System. International Rice Research Institute, Manila, Philippines. pp. 11-30.
30 Peakall, R. and P.E. Smouse. 2006. GENALEX 6: genetic analysis in Excel Population genetic software for teaching and research. Mol. Ecol. Notes 6:288-295.   DOI   ScienceOn
31 Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nat. Biotechnol. 18: 233-234.   DOI   ScienceOn
32 Qu, S., G. Liu, B. Zhou, M. Bellizzi, L. Zeng, L. Dai, B. Han and G.L. Wang. 2006. The broadspectrum blast resistance gene pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics 172:1901-1914.   DOI
33 Sasaki, T. and B. Burr. 2000. International Rice Genome Sequencing Project: The effort to completely sequence the rice genome. Curr. Opin. Plant Biol. 3:138-141.   DOI   ScienceOn
34 Shang, J., Y. Tao, X. Chen, Y. Zou, C. Lei, J. Wang, X. Li, X. Zhao, M. Zhang, Z. Lu, J. Xu, Z. Cheng, J. Wan and L. Zhu. 2009. Identification of a new rice blast resistance gene, Pid3, by genomewide comparison of paired nucleotide-inbding site-leucine-rich repeat genes and their pseudogene alleles between the two equensced rice genomes. Genetics 182:1303-1311.   DOI   ScienceOn
35 Suh, J.P., J.H. Roh, Y.C. Cho, S.S. Han, Y.G. Kim and K.K. Jena. 2009. The Pi40 gene for durable resistance to rice blast and molecular analysis of Pi40-advanced backcross breeding lines. Phytopathology 99:243-250.   DOI   ScienceOn
36 Talbot, N.J. and A.J. Foster. 2001. Genetics and genomics of the rice blast fungus Magnaporthe grisea: developing an experimental model for understanding fungal diseases of cereals. Adv. Bot. Res. 34:263-287.   DOI
37 Yaegashi, H. 1994. Use of resistant varieties and disease control for paddy rice. Agric. Hortic. 69:149-154.
38 Talbot, N.J. 2003. On the trail of a cereal killer: investigating the biology of Magnaporthe grisea. Annu. Rev. Microbiol. 57: 177-202.   DOI   ScienceOn
39 Zeigler, R.S., J. Thome, J. Nelson, M. Levy and F.J. Correa-Victoria. 1994. Lineage exclusion: A proposal for linking blast population analysis to resistance breeding: In Zeigler R.S., S.A Leong and P. Teng (eds.), Rice Blast Disease, CAB International, Wallingford, UK. pp. 267-292.
40 Villa, T.C.C., N. Maxted, M. Scholten and B. Ford-Lloyd. 2006. Defining and identifying crop landraces. Plant Genet. Resour. 3:373-384.
41 Yamanaka, S. and T. Yamaguchi. 1987. Rice Blast Disease. Yokendo, Tokyo, Japan. p. 365 (in Japanese).
42 Zhai, C., F. Lin, Z. Dong, X. He, B. Yuan, X. Zeng, L. Wang and Q. Pan. 2011. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication. New Phytol. 189:321-334.   DOI   ScienceOn
43 Zhou, B., S. Qu, G. Liu, M. Dolan, H. Sakai, G. Lu , M. Bellizzi and G.L. Wang. 2006. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol. Plant Microbe Interact. 19:1216-1228.   DOI   ScienceOn
44 Couch, B.C. and L.M. Kohn. 2002. A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea. Mycologia 94:683-693.   DOI   ScienceOn