• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.42.2-42.2
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• 2019

Turbulence is one of the natural phenomena in molecular clouds. It affects gas density and velocity fluctuation within the molecular clouds and controls the mode and tempo of star formation. However, despite many years of study, the properties of turbulence remain poorly understood. As part of the Taeduk Radio Astronomy Observatory (TRAO) Key Science Program (KSP), "mapping Turbulent properties In star-forming MolEcular clouds down to the Sonic scale (TIMES; PI: Jeong-Eun Lee)", we have fully mapped two star-forming molecular clouds, the Orion A and the Ophiuchus molecular clouds, in 3 sets of lines ($^{13}CO$ J=1-0, $C^{18}O$ J=1-0, HCN J=1-0, $HCO^+$ J=1-0, CS J=2-1, and $N_2H^+$ J=1-0) using the TRAO 14-m telescope. We apply a statistical analysis, Principal Component Analysis (PCA), which can recover an underlying turbulent-power spectrum from an observed P-P-V spectral map. We compare turbulence properties not only between the two clouds, but also between different parts within each cloud. We present the first result of our observation program.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.1
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• pp.37-44
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• 2011

Parthenogenetic embryonic stem (pES) cells could provide a valuable model for research into genomic imprinting and X-linked diseases. In this study, pES cell lines were established from oocytes of hybrid offspring of Kunming and 129/Sv mice, and pluripotency of pES cells was evaluated. The pES cells maintained in the undifferentiated state for more than 50 passages had normal karyotypes with XX sex chromosomes and exhibited high activities of alkaline phosphatase (AKP) and telomerase. Meanwhile, these cells expressed ES cell molecular markers SSEA-1, Oct-4, Nanog, and GDF3 but not SSEA-3 detected by immunohistochemistry and RT-PCR. The pES cells could be differentiated into various types of cells from three germ layers in vitro by analysis of embryoid bodies (EBs) with immunohistochemistry and RT-PCR, and in vivo by observation of pES cell-derived teratoma sections. Therefore, the established pES cell lines contained all features of mouse ES cells. This work provides a new strategy for isolating pES cells from Kunming mice, and the pES cell lines could be applied as the cell model in research into genomic imprinting and epigenetic regulation of Kunming mice.

• BMB Reports
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• v.34 no.3
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• pp.189-193
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• 2001

Curcumin a yellow pigment from Curcuma Tonga, has been known to possess antioxidative and anticarcinogenic properties, as well as to induce apoptosis in some cancer cells. There have been, however, several contradictory reports that hypothesized curcumin (a hydrophobic molecule) can bind a membrane Gpid bilayer and induce nonspecific cytotoxicity in some cell lines. Why curcumin shows these contradictory effects is unknown. In A-431 cells, growth inhibition by curcumin is due mostly to the specific inhibition of the intrinsic tyrosine kinase activity of the epidermal growth factor receptor, as reported earlier by Korutla et al. Thus, we assumed that the cell death of A-431 by curcumin might be due to the specific induction of apoptosis. In this paper we clearly show that curcumin induces apoptosis in A-431 cells. The cureumin-induced cell death of A-431 exhibited various apoptotic features, including DNA fragmentation and nuclear condensation. Furthermore, the curcumin-induced apoptosis of A-431 cells involved activation of caspase-3-like cysteine protease. Involvement of caspase-3 was further confirmed by using a caspase-3 specific inhibitor, DEVD-CHO. In another study, decreased nitric oxide (NO) production was also shown in A-431 cells treated with curcumin, which seems to be the result of the inhibition of the iNOS expression by curcumin, as in other cell lines. However, 24 h after treatment of curcumin there was increased NO production in A-431 cells. This observation has not yet been clearly explained. We assumed that the increased NO production may be related to denitrosylation of the enzyme catalytic site in caspase-3 when activated. Taken together, this study shows that the cell death of A-431 by curcumin is due to the induction of apoptosis, which involves caspase-3 activation.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.47.1-47.1
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• 2016

We present a preliminary result of search for CO molecular outflows toward a sample of 68 candidate Very Low Luminosity Objects (VeLLOs; Lint ${\leq}0.1L_{\odot}$) to help to understand their physical properties. The sources have been identified using the data at IR to radio wavelengths by M. Kim et al. 2016 toward nearby star-forming regions in the Gould belt. These sources were observed in rotational transitions 2-1 and 3-2 of $^{12}CO$, $^{13}CO$, and $C^{18}O$ molecules with SRAO, CSO, JCMT, and ASTE telescopes. In the beginning of our survey we made a single pointing observation in $^{12}CO$ 2-1 or 3-2 lines for our sample, identifying 53 sources as potential outflow candidates from their line wing features. We made full or partial mapping observations for these candidates with the same lines, finding 33 sources with bipolar or one-sided outflow features. Out of these 33 sources, 6 VeLLOs are previously known sources to have their outflows and 27 VeLLOs are found to be new outflow sources identified from this study. We estimated outflow properties with corrections for excitation temperature, optical depth, and inclination. Their outflow forces range from $8.7{\times}10^{-10}$ to $6.0{\times}10^{-5}M_{\odot}\;km\;s^{-1}yr^{-1}$ with a median value of $3.6{\times}10^{-7}M_{\odot}\;km\;s^{-1}yr^{-1}$, indicating that most of the VeLLOs are less powerful than those for protostars. Their accretion luminosities vary from $9.7{\times}10^{-9}$ to $166L_{\odot}$ with a median value of $0.004L_{\odot}$, implying that most VeLLOs have larger ratios of the accretion luminosity to the internal luminosity but a significant number of VeLLOs have smaller ratios. This result suggests that many of the VeLLOs can be explained with episodic accretion but a significant number of VeLLOs cannot.

• YAKHAK HOEJI
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• v.55 no.1
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• pp.49-55
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• 2011

Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) is one of the promising anti-cancer agent because of its ability to selectively induce apoptosis in tumor cell lines but not in normal cells. However, TRAIL resistance has been reported in some cancer cells including hepatocarcinoma cells. Therefore, studies of agents that sensitize TRAIL-resistant cancer cells could be a effective therapeutic approach in cancer management. In our study, we examined the effect of combination of TRAIL with apigenin in human hepatocellular carcinoma cells. As a result, the combined use of TRAIL and apigenin significantly enhanced the cytotoxicity in PLC-PRF5 cells. Flow cytometry analysis after annexin V-FITC/PI dual staining showed that this increase of cell cytotoxicity was related to enhanced apoptosis in combined treatment of TRAIL with apigenin. Furthermore, synergistic induction of apoptosis was also confirmed by observation of morphological changes and annexin V-FITC/PI fluorescence. Our findings suggests that apigenin has the potential to improve the efficiency of TRAIL-based therapies in human hepatocellular carcinoma cells. Further study is needed to reveal the molecular mechanisms of this combined therapy.

• Mass Spectrometry Letters
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• v.4 no.3
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• pp.41-46
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• 2013

The goal of this study is to find an experimental condition which enables us to perform enzymatic studies on the cellular behavior of PTEN (phosphatase and tensine homolog) through identification of molecular species of phosphatidylinositol 3,4,5-trisphosphates and their quantitative analysis in a mammalian cell line using mass spectrometry. We initially exployed a two-step extraction process using HCl for extraction of phosphatidylinositol 3,4,5-trisphosphates from two mammalian cell lines and further analyzed the extracted phosphatidylinositol 3,4,5-trisphosphates using tandem mass spectrometry for the identification of them. We finally quantified the concentration of phosphatidylinositol 3,4,5-trisphosphates using internal standard calibration. From these observation, we found that HEK 293-T cells is a good model to examine the enzymatic behavior of PTEN in a cell, and the minimum amount of phosphatidylinositol 3,4,5-trisphosphates is more than 50 pmol for quantification in a mass spectrometer. These results suggest that the well-optimized experimental conditions are required for the investigation of the cellular PTEN in terms of the catalytic mechanism and further for the detailed identification of cellular substrates.

• Journal of Astronomy and Space Sciences
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• v.19 no.1
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• pp.7-18
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• 2002

Interstellar cabon monoxide and its isotopes were observed with the 13.7m radio telescope of the Daeduk Radio Astronomy Observatory toward Orion Molecular Cloud-1 (OMC-1). We derive the excitation temperature, optical depth, column density, and isotopic abundance ratios from the observed lines of $^{12}CO,^{13}CO,\;and\;C^{18}O$ inside of the region of $11'{\times}11'$. The optical depths of $^{13}CO$ were obtained to be 0.1~0.4, while those of $C^{18}O$ to be 0.01~0.03. The isotopic ratios $^{12}C/^{13}C$ were also estimated to be 2~60. We could not find the radial isotopic ratio $^{12}C/^{13}C$ gradient in the OMC-1.

• Korean Journal of Materials Research
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• v.21 no.11
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• pp.634-638
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• 2011

We report growth of epitaxial AlN thin films on c-plane sapphire substrates by plasma-assisted molecular beam epitaxy. To achieve two-dimensional growth the substrates were nitrided by nitrogen plasma prior to the AlN growth, which resulted in the formation of a two-dimensional single crystalline AlN layer. The formation of the two-dimensional AlN layer by the nitridation process was confirmed by the observation of streaky reflection high energy electron diffraction (RHEED) patterns. The growth of AlN thin films was performed on the nitrided AlN layer by changing the Al beam flux with the fixed nitrogen flux at 860$^{\circ}C$. The growth mode of AlN films was also affected by the beam flux. By increasing the Al beam flux, two-dimensional growth of AlN films was favored, and a very flat surface with a root mean square roughness of 0.196 nm (for the 2 ${\mu}m$ ${\times}$ 2 ${\mu}m$ area) was obtained. Interestingly, additional diffraction lines were observed for the two-dimensionally grown AlN films, which were probably caused by the Al adlayer, which was similar to a report of Ga adlayer in the two-dimensional growth of GaN. Al droplets were observed in the sample grown with a higher Al beam flux after cooling to room temperature, which resulted from the excessive Al flux.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.6
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• pp.1009-1017
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• 2001

The acousto-optical spectrometer is designed by using 1 GHz bandwidth acousto-optic deflector for radio signal analysis. This system is a high resolution wide band spectrometer which uses I GHz bandwidth and a total of 2,048 channel charge coupled device. When we measured the spectrums of signals deflected by acousto-optical spectrometer, we confirmed the stability of the total system by repetitive observations of the same frequency, and each part of the system worked well. We installed this system onto 60 cm radio telescope, and observed 12CO(J= 1 ∼0) emission lines around CRL 2688, IRC 10216 and NGC 5005 Galaxy center. We could observe effectively very narrow band width radio spectrum as well as wide band radio spectrum. We also confirmed high sensitivity and resolution in observation of 12CO(J-10) omission line of NGC 5005 Galaxy center which is a weak signal.

• Animal cells and systems
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• v.4 no.3
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• pp.273-278
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• 2000

Transforming growth factor-$\beta$ (IGF-$\beta$)s are multifunctional small polypeptides synthesized in most cell types. TGF-$\beta$ exerts pivotal effects on both bone formation and resorption. In addition, increasing lines of evidence implicate TGF-$\beta$ as a potential coupling factor between these two processes during bone remodeling. In the present study, the expression form and the activation mechanism of latent-TGF-$\beta$ were investigated using specific antibodies for each isoform. TGF-$\beta$s were observed to be synthesized and accumulated in a large amount in cultured osteoblastic cells. The estimated molecular weights of intracellular TGF-$\beta$2 and -$\beta$3 were 49 and 55 kDa, respectively. Based on proteolytic digestion study and immunofluorescence observation, these precursor forms seemed to be accumulated in distinct intracellular compartments. To examine whether the internal pool of TGF-$\beta$ was possiblely regulated by external signals, their biological activites were examined in a conditioned media of this cell. Although the intact conditioned media did not contain detectable TGF-$\beta$ activity, heat-treatment or acid-activation of the conditioned media revealed significant TGF-$\beta$ activity. Furthermore, in the presence of estrogen, this activity was dramatically diminished. It is known that activation of latent TGF-$\beta$ can be achieved by different chemical and enzymatic treatments, or by incubation with certain cell types. This extracellular activation was suggested as a key step in the regulation of TGF-$\beta$ activity. In addition to these extracellular activation, this study suggests that the synthesis and intracellular processing are important regulation steps for TGF-$\beta$ action. In addition, this regulation Is specific for TGF-$\beta$ type 2, because the change was not observed in TGF-$\beta$3 in osteoblastic cell line.