• Molecular & Cellular Toxicology
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• v.4 no.2
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• pp.164-169
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• 2008

Methylmercury (MeHg) is known to have devastating effects on the mammalian nervous system. In order to characterize the mechanism of MeHg-induced neurotoxicity, we investigated the analysis of transcriptional profiles on human 8k cDNA microarray by treatment of $1.4{\mu}M$ MeHg at 3, 12, 24 and 48h in human neuroblastoma SH-SY5Y cell line. Some of the identified genes by MeHg treatment were significant at early time points (3h), while that of others was at late time points (48h). The early response genes that may represent those involved directly in the MeHg response included pantothenate kinase 3, a kinase (PRKA) anchor protein (yotiao) 9, neurotrophic tyrosine kinase, receptor, type 2 gene, associated with NMDA receptor activity regulation or perturbations of central nervous system homeostasis. Also, when SH-SY5Y cells were subjected to a longer exposure (48h), a relative increase was noted in a gene, glutamine-fructose-6-phosphate transaminase 1, reported that overexpression of this gene may lead to the increased resistance to MeHg. To confirm the alteration of these genes in cultured neurons, we then applied real time-RT PCR with SYBR green. Thus, this result suggests that a neurotoxic effect of the MeHg might be ascribed that MeHg alters neuronal receptor regulation or homeostasis of neuronal cells in the early phase. However, in the late phase, it protects cells from neurotoxic effects of MeHg.

• Genomics & Informatics
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• v.4 no.1
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• pp.11-15
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• 2006

The aim of this study is to identify hRad21-binding sites in human chromosome, the core component of cohesin complex that held sister chromatids together. After chromatin immunoprecipitation with an hRad21 antibody, it was cloned the recovered DNA and sequenced 30 independent clones. Among them, 20 clones (67%) contained repetitive elements including short interspersed transposable elements (SINE or Alu elements), long terminal repeat (LTR) and long interspersed transposable elements (LINE), fourteen of these twenty (70%) repeats clones had Alu elements, which could be categorized as the old and the young Alu Subfamily, eleven of the fourteen (73%) Alu elements belonged to the old Alu Subfamily, and only three Alu elements were categorized as young Alu subfamily. There is no CpG island within these selected clones. Association of hRad21 with Alu was confirmed by chromatin immunoprecipitation-PCR using conserved Alu primers. The primers were designed in the flanking region of Alu, and the specific Alu element was shown in the selected clone. From these experiments, it was demonstrated that hRad21 could bind to SINE, LTRs, and LINE as well as Alu.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.33.2-34
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• 2016

The unification model of active galactic nuclei invokes the presence of a thick molecular torus that hides the broad emission line region from a line of sight toward observers with low latitude. It is expected that the illuminated side of the molecular torus may be photodissociated by strong far UV radiation from the central AGN, forming an H I region with a high neutral column density. We propose that the Rayleigh scattering optical depth of this HI region can be significant for most broad $Ly{\alpha}$ line photons with the Doppler factor not exceeding 104 km s-1. Rayleigh scattered $Ly{\alpha}$ photons can be characterized by strong linear polarization depending on their scattering optical depth. We performed Monte Carlo simulations of polarized radiative transfer of $Ly{\alpha}$ adopting simple scattering geometries relevant to the unification model of AGN. We find that for a low torus the Rayleigh scattered $Ly{\alpha}$ is polarized in the direction parallel to the symmetry axis with the polarization degree dependent on wavelength. In the case of a high torus, the core part of $Ly{\alpha}$ is polarized in the direction perpendicular to the symmetry axis whereas the wing part is parallelly polarized. We conclude that careful spectropolarimetry around $Ly{\alpha}$ can be useful in testing the AGN unification model.

• Toxicological Research
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• v.27 no.1
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• pp.43-48
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• 2011

Even though exposure to benzene has been linked to a variety of cancers including leukemia, the detailed molecular mechanisms relevant to benzene-induced carcinogenesis remain to be clearly elucidated. In this study, we evaluated the effects of benzene on differential gene expression in a leukemia cell line. The K562 leukemia cell line used in this study was cultured for 3 h with 10 mM benzene and RNA was extracted. To analyze the gene expression profiles, a 41,000 human whole genome chip was employed for cDNA microarray analysis. We initially identified 6,562 genes whose expression was altered by benzene treatment. Among these, 3,395 genes were upregulated and 3,167 genes were downregulated by more than 2-fold, respectively. The results of functional classification showed that the identified genes were involved in biological pathways including transcription, cell proliferation, the cell cycle, and apoptosis. These gene expression profiles should provide us with further insights into the molecular mechanisms underlying benzene-induced carcinogenesis, including leukemia.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.60.1-60.1
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• 2010

Methanol maser sources are believed to represent the earliest evolutionary stage of massive star formation. Pandian et al. (2007) recently made a very sensitive blind survey of 6.7GHz ClassII methanol maser towards the Galacitic midplane ($35^{\circ}$<1<$55^{\circ}$ & |b|<$0.5^{\circ}$) and found 86 maser sources. We carried out 22GHz $H_2O$ and 44.0 GHz $CH_3OH$ maser line surveys of them with KVN 21m and various (CO (2-1), $^{13}CO$ (2-1), $^{13}CO$ (1-0), $C^{18}O$ (2-1), CS (2-1), $HCO^+$ (3-2) and HCN (3-2)) molecular line surveys with SRAO 6m and TRAO 14m. we will present the preliminary results and discuss the implications for massive star formation.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.2
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• pp.189-194
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• 2004

Amylase isozyme based three multivoltine viz., N+p, Np, N+ $p^{cho}$ and two bivoltine-D6+p, D6p syngenic lines (Syn. L) were developed from germplasm (GP) stocks Nistari (N) and D6 respectively. haemolymph isozyme pattern at pH 7.0 and 8.5 depicted a total 11 number (Am $y_{1 to 6}$ at pH 7.0 and Am $y^{l to 5}$ at pH 8.5) of native proteins (NP) of various sizes are amylase isozyme expressers. Among eleven NPs, two NPs of 770 kDa (Am $y^{6}$ at pH 7.0) and 376 kDa (Am $y^3$ at pH 8.5) are $\alpha$-amylase expressers and remaining NPs of 370, 364, 350, 329 and 274 kDa at pH 7.0 and 206, 292, 416, 725 kDa at pH 8.5 are $\beta$-amylase expressers. Accordingly, digestive juice amylase isozyme pattern at aforesaid pH also depicted a total number of 10 NPs (Am $y^{1 to 5}$) at each pH 7.0 and 8.5 are amylase expressers of which NP of 387 kDa (Am $y^4$ at pH 7.0) and 780 kDa (Am $y^{5}$ at pH 8.5) are a-amylase expresser. Remaining NPs of 338,297 ＆ 216 kDa at pH 7.0 and 370, 341, 329 ＆302 kDa at pH 8.5 are $\beta$-amylase expresser. Recurrent backcross lines (RBL) viz., N+pRBL and NpRBL were developed through introgression of high shell weight character (a multigenic trait) to be used further for congenic line (Con. L) development and to understand any association with introgressed character. Isozyme pattern in haemolymph of RBLs depicted only one $\alpha$-amylase of 770 kDa at pH 7.0 and 376 kDa at pH 8.0 with three and four respective $\beta$-amylase bands but in bivoltine lines numbers of $\beta$-amylase bands vary between 1 to 2 at aforesaid pH. Variability was also observed in digestive juice of multivolitine and its RBLs but bivoltine lines express null activity at both pH except appearance of one very week $\alpha$-amylase band D6+p at pH 8.5. Overall study suggests that not a single NP at both pH is common for expression of any band of amylase isozyme i.e., a totally different set of proteins are the amylase isozyme expresser at specific pH and no molecular factor of amylase is associated in developed RBLs which showed improvement on survival, single cocoon shell weight (SCSW) and single filament length over receptor parents.s.s.s.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.302-307
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• 1999

Hamlet (PI549276) possessing 2RL was obtained by cross between a wheat cultivar ND7532 (Froid/Centurk) and a rye cultivar Chaupon. Chaupon was known to have resistant gene to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. The wheat-rye translocation line (Coker797*4/Hamlet) was also known to be resistant to biotype L of Hessian fly larvae. We analysed a set of 96 ESTs from the wheat-rye translocation line (2BS/2RL). ESTs were classified by various physiological processings, such as primary metabolism, secondary metabolism, transcription, translation, transport, signal transduction, defense, transposable element, and others. Three sequences encoding thioredoxin peroxidase, 26S rRNA, and rubisco small subunits were homologous to registered genes in rye. Although limited number of clones were used to develop ESTs, these clones and their sequence information may be useful for researchers studying general physiology and molecular biology on the translocation line.

• The Bulletin of The Korean Astronomical Society
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• v.21
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• pp.12.2-13
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• 1996

• The Bulletin of The Korean Astronomical Society
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• v.31 no.2
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• pp.70.1-70.1
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• 2006

• Journal of The Korean Astronomical Society
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• v.34 no.1
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• pp.17-24
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• 2001

We have studied the central parts of M82, which is a well-known infrared luminous, starburst galaxy, by analyzing archival data from the Infrared Space Observatory (ISO). M82 was observed at 11 positions covering $\pm$45" from the center along the major axis. We analyzed 4 emission lines, [ArIII] 8.99 ${\mu}m$, $H_2$ 17.034 ${\mu}m$, [FeII] 25,98 ${\mu}m$, and [SiII] 34,815 ${\mu}m$ from $SWSO_2$ data. The integrated flux distributions of these lines are quite different. The $H_2$ line shows symmetric twin peaks at $\~$18" from the center, which is a general characteristic of molecular lines in starburst or barred galaxies. This line appears to be associated with the rotating molecular ring at around $\~$200 pc just outside the inner spiral arm. The relative depletion of the $H_2$ line at the center may be due to the active star formation activity which dissociates the $H_2$ molecules. The other lines have peaks at the center and the distributions are nearly symmetric. The line profiles are deconvolved assuming that both intrinsic and instrumental profiles are Gaussian. The velocity dispersion outside the core is found to be $\~50 km s^{-1}$. The central velocity dispersion is much higher than $50 km s^{-1}$, and different lines give different values. The large central velocity dispersion ($\sigma$) is mostly due to the rotation, but there is also evidence for a high $\sigma$ for [ArIII] line. We also generated position-velocity maps for these four lines. We found very diverse features from these maps.