• 한국진공학회:학술대회논문집
• /
• 한국진공학회 1995년도 제8회 학술발표회 논문개요집
• /
• pp.073-74
• /
• 1995

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 1997년도 제12회 학술발표회 논문개요집
• /
• pp.164-164
• /
• 1997

• 컴퓨터교육학회논문지
• /
• 제9권4호
• /
• pp.63-74
• /
• 2006

분자 다킹은 신약, 신소재, 고분자의 개발 과정에서 대규모의 화학분자 데이터베이스의 화학분자 데이터들을 실제 실험을 통하지 않고 시뮬레이션을 통해 한정된 화학 분자만을 스크링하는 과정이다. 분자 다킹은 대규모 컴퓨팅 파워와 데이터 저장 용량을 요구하는 대표적인 대규모의 과학 어플리케이션이다. 기존의 분자 다킹 어플리케이션들은 슈퍼컴퓨터, 클러스터, 워크스테이션 등을 이용하여 작업을 수행하도록 개발되었다. 하지만 슈퍼컴퓨터를 이용한 분자 다킹은 너무 많은 비용이 든다는 문제점이 있고, 클러스터나 워크스테이션을 이용한 분자 다킹은 오랜 수행 시간이 요구된다는 문제점을 가지고 있다. 이에 본 논문에서는 그리드 서비스 기반 분자 다킹 어플리케이션을 제안하였다. 이를 위해 본 논문에서는 효율적인 분자 다킹 서비스를 제공하기 위해 자원 브로커와 데이터 브로커를 설계하고, 분자 다킹을 위한 다양한 그리드 서비스들을 개발하였다.

• Membrane and Water Treatment
• /
• 제10권3호
• /
• pp.207-212
• /
• 2019

Non-aqueous solvents (NASs) are generally known to be barely miscible, and reactive with polar compounds, such as water. However, water can interact with some NASs, which can be used as a new means for water recovery from saline water. This study explored the fate of water and salt in NAS, when saline water is mixed with NAS. Three amine solvents were selected as NAS. They had the same molecular formula, but were differentiated by their molecular structures, as follows: 1) NAS 'A' having the hydrophilic group ($NH_2$) at the end of the straight carbon chain, 2) NAS 'B' with symmetrical structure and having the hydrophilic group (NH) at the middle of the straight carbon chain, 3) NAS 'C' having the hydrophilic group ($NH_2$) at the end of the straight carbon chain but possessing a hydrophobic ethyl branch in the middle of the structure. In batch experiments, 0.5 M NaCl water was blended with NASs, and then water and salt content in the NAS were individually measured. Water absorption efficiencies by NAS 'B' and 'C' were 3.8 and 10.7%, respectively. However, salt rejection efficiency was 98.9% and 58.2%, respectively. NAS 'A' exhibited a higher water absorption efficiency of 35.6%, despite a worse salt rejection efficiency of 24.7%. Molecular dynamic (MD) simulation showed the different interactions of water and salts with each NAS. NAS 'A' formed lattice structured clusters, with the hydrophilic group located outside, and captured a large numbers of water molecules, together with salt ions, inside the cluster pockets. NAS 'B' formed a planar-shaped cluster, where only some water molecules, but no salt ions, migrated to the NAS cluster. NAS 'C', with an ethyl group branch, formed a cluster shaped similarly to that of 'B'; however, the boundary surface of the cluster looked higher than that of 'C', due to the branch structure in solvent. The MD simulation was helpful for understanding the experimental results for water absorption and salt rejection, by demonstrating the various interactions between water molecules and the salts, with the different NAS types.

• Journal of Microbiology and Biotechnology
• /
• 제18권10호
• /
• pp.1672-1677
• /
• 2008

IscR (iron-sulfur cluster regulator) has been reported to be a repressor of the iscRSUA operon, and in vitro transcription reactions have revealed that IscR has a repressive effect on the iscR promoter in the case of [$Fe_{2}S_{2}$] cluster loading. In the present study, the iscR gene from A. ferrooxidans ATCC 23270 was cloned and successfully expressed in Escherichia coli, and then purified by one-step affinity chromatography to homogeneity. The molecular mass of the IscR was 18 kDa by SDS-PAGE. The optical and EPR spectra results for the recombinant IscR confirmed that an iron-sulfur cluster was correctly inserted into the active site of the protein. However, no [$Fe_{2}S_{2}$] cluster was assembled in apoIscR with ferrous iron and sulfide in vitro. Therefore, the [$Fe_{2}S_{2}$] cluster assembly in IscR in vivo would appear to require scaffold proteins and follow the Isc "AUS" pathway.

• 한국임상수의학회지
• /
• 제23권4권
• /
• pp.405-410
• /
• 2006

피부사상균증이 있는 개와 고양이에서 분리한 9주의 Microsporum canis와 5주의 Microsporum gypseum에서 ribosomal DNA를 추출하여 internal transcribed spacer 1 (ITS1) gene을 PCA로 증폭한 후 sequencing을 실시하여, 각 사상균의 계통학적 관계를 조사하였다. M canis 분리주 9주의 ITS1 gene의 nucleotide sequence는 100% 일치하였으며 M gypseum 분리주 5주의 nucleotide sequence도 100% 일치하였다. M canis 분리주 9주의 계통분석 결과 미국, 일본, 호주 및 유럽에서 분리된 M canis와 같은 cluster에 속하였으며 다른 Microsporum spp와는 유전적으로 다른 cluster를 형성하였다. 그러나 M canis와 M distortum, M equinum, M ferrugineum은 유전적으로 매우 가까운 위치에 있었다. M gypseum 분리주는 M canis와는 다른 cluster를 형성하였다. ITS1 gene의 분자생물학적 분석은 Microsporum spp를 확인하고 그들의 유전학적 관계를 이해하는 유용한 정보를 제공하는 것으로 생각된다.

• 한국균학회지
• /
• 제27권4호통권91호
• /
• pp.274-279
• /
• 1999

먹물버섯속 및 눈물버섯속 균류의 계통학적 유연관계 분석을 위해 rDNA의 cluster 중 ITS 영역을 Polymerase Chain Reaction(PCR)로 증폭하여 염기서열을 밝혔다. 이들의 ITS I, II 영역은 각각 $258{\sim}301\;bp,\;253{\sim}275\;bp$의 염기쌍으로 이루어져 있었으며 균주간 ITS 염기서열의 상동성은 $43.9{\sim}96%$로 나타났다. 이들의 염기서열을 이용해 분류도(tree)를 작성한 결과, Singer의 형태적 분류체계와 거의 유사한 결과를 보였으나 눈물버섯속 균류들은 먹물버섯속 내 그룹 II의 종들과 함께 분지를 형성하였고 먹물버섯속의 의기준종(type species)인 C. comatus는 조사된 다른 동일 속의 종들과의 유사도에서 50%수준의 저조한 상동성을 보여주었다. 이와 같은 결과로 볼 때 먹물버섯류가 기존의 다수 학자들이 보고한 단일 계통진화(monophyletic evolution)를 해온 속이란 점에서 의문점을 남기었고 좀더 많은 조사가 필요하겠으나 눈물버섯속 균류도 먹물버섯속 균류와 분리시키는 것보다는 동일 속으로 분류하는 것이 바람직 할 것으로 사료되었다.

• 한국패류학회지
• /
• 제29권3호
• /
• pp.163-169
• /
• 2013

In the present study, the seven primers BION-33, BION-34, BION-37, BION-41, BION-44, BION-45 and BION-42 generated the total number of loci, average number of loci per lane and specific loci in Hongseong, Yeosu and Goheung population of F. mutica, respectively. 7 primers generated 19 specific loci in the Hongseong population, 29.3 in the Yeosu population and 23.1 in the Goheung population, respectively. Especially, the decamer primer BION-37 generated 7 unique loci to each population, which were identifying each population, approximately 700 bp in Hongseong population. In this study, the dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (HONGSEONG 01-HONGSEONG 07), cluster 2 (YEOSU 08-YEOSU 14) and cluster 3 (GOHEUNG 15-GOHEUNG 21). Among the twenty one cockles, the shortest genetic distance that displayed significant molecular differences was between individuals 17 and 19 from the Goheung population (genetic distance = 0.051), while the longest genetic distance among the twenty-one cockle individuals that displayed significant molecular differences was between individuals HONGSEONG no. 03 and YEOSU no. 12 (genetic distance = 0.616). Relatively, individuals of YEOSU population were fairly closely related to that of GOHEUNG population. Ultimately, PCR fragments revealed of in this study may be useful as a DNA marker the three geographic populations to distinguish.

• 한국발생생물학회지:발생과생식
• /
• 제23권2호
• /
• pp.193-198
• /
• 2019

Genomic DNA samples were obtained from cultured penaeid shrimp (Penaeus chinensis) individuals such as fresh shrimp population (FSP) and deceased shrimp population (DSP) from Shinan regions in the Korean peninsula. In this study, 233 loci were identified in the FSP shrimp population and 162 in the DSP shrimp population: 33 specific loci (14.2%) in the FSP shrimp population and 42 (25.9%) in the DSP population. A total of 66 (an average of 9.4 per primer) were observed in DSP shrimp population, whereas 55 unique loci to each population (an average of 7.9 per primer) in the FSP shrimp population. The Hierarchical dendrogram extended by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (FRESH 01, 02, and DECEASED 12, 13, 15, 16, 17, 19, 20, 22) and cluster 2 (FRESH 03, 04, 05, 06, 07, 08, 09, 10, 11, and DECEASED 14, 18, 21). Among the twenty-two shrimp, the shortest genetic distance that exposed significant molecular differences was between individuals 20 and 16 from the DSP shrimp population (genetic distance=0.071), while the longest genetic distance among the twenty-two individuals that established significant molecular differences was between individuals FRESH no. 02 and FRESH no. 04 (genetic distance=0.477). In due course, PCR analysis has revealed the significant genetic distance among two penaeid shrimp populations.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2006년도 추계학술대회 및 정기총회
• /
• pp.32-44
• /
• 2006

To investigate non-aflatoxin-production of A. oryzae at the molecular level, an aflatoxin biosynthesis gene homolog cluster of RIB 40 was analyzed. Although most genes in the corresponding cluster exhibited from 97 to 99 % similarity to those of Aspergillus flavus, three genes shared 93 % similarity or less. In addition, although slight expression of aflR, positive transcriptional regulator gene, was detected in some A. oryzae strains having seven aflatoxin biosynthesis homologous genes, other genes related to aflatoxin production were not detected. RIB strains were mainly divided into group 1, having seven aflatoxin biosynthesis homologous genes (aflT, nor-i, aflR, norA, avnA, verB, and vbs), and group 2, having three homologous (avnA, verB, and vbs). Partial aflatoxin homologous gene cluster of RIB62 from group 2 was sequenced and compared with that of RIB40 from group 1. RIB62 showed a large deletion upstream of ver-1 with more than half of the aflatoxin homologous gene cluster missing including aflR, a positive transcriptional regulatory gene. Adjacent to the deletion of the aflatoxin homologous gene cluster, RIB62 has a unique sequence of about 8kb and a telomere. Southern analysis of A. oryzae RIB strains with four kinds of probe derived from the unique sequence of RIB62 showed that all group 2 strains have identical hybridizing signals. Polymerase chain reaction with specific primer set designed to amplify the junction between ver-1 and the unique sequence of RIB62 resulted in the same size of DNA fragment only from group 2 strains. Based on these results, we developed a useful genetic tool that distinguishes A. oryzae group 2 strains from the other groups' strains and propose that it might have differentiated from the ancestral strains due to chromosomal breakage.