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Molecular Phylogenetic Classification of Dermatophytes Isolated from Dogs and Cats  

Kim, Doo (School of Veterinary Medicine & Institute of Veterinary Medicine, Kangwon National University)
Jeoung, Seok-Young (School of Veterinary Medicine & Institute of Veterinary Medicine, Kangwon National University)
Ahn, So-Jeo (School of Veterinary Medicine & Institute of Veterinary Medicine, Kangwon National University)
Publication Information
Journal of Veterinary Clinics / v.23, no.4, 2006 , pp. 405-410 More about this Journal
Abstract
Using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences from 9 strains of Microsporum canis and 5 strains of Microsporum gypseum isolated from dogs and a cat with dermatophytosis, we demonstrated the mutual phylogenetic relationship of these strains. Nucleotide sequence analysis of the ITS 1 gene fragments from the 9 strains of M canis had the 100% nucleotide sequence similarities and the 5 strains of M gypseum also had the 100% nucleotide sequence similarities. The phylogenetic analysis of the nucleotide sequences of the 9 strains of M canis formed a nested cluster with the reference strains of M canis originating from USA, Australia, Japan, and Europe. M canis were genetically distinct from the other reference strains of Microsporum spp, but M distortum, M equinum, and M. ferrugineum were genetically very close to M canis. M gypseum from a cluster in the phylogenetic tree with M canis as an outgroup. The molecular analysis of ITS 1 genes provided the useful information for the identification of these microsporum species and the understanding of their relationship.
Keywords
Microsporum spp; ITS1; phylogeny; dog and cat;
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1 Graser Y, Kuijpers AFA, Presber W, de Hoog GS. Molecular taxonomy of the Trichophyton rubrum complex. J Clin Microbiol 2000; 38: 3329-3336
2 Berbee ML, Yoshimura A, Sugiyama J, Taylor JW. Is penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8S and ITS ribosomal DNA sequence data. Mycologia 1995; 87: 210-222   DOI
3 Carbone I, Kohn LM. Ribosomal DNA sequence divergence within internal transcribed spacer 1 of the Sclerotiniaceae. Mycologia 1993; 85: 415-427   DOI
4 Kaszubiak A, Klein S, de Hoog GS, Graser Y. Population structure and evolutionary origens of Microsporum canis, M ferrugineum and M audouinii. Infection, Genetics and Evolution 2004; 4: 179-186   DOI   ScienceOn
5 Kawasaki M, Aoki M, Ishizaki H, Nishimura K, Miyaji M. Phylogeny of Epidermophyton floccosum and other dennatophytes. Mycologia 1996; 134: 121-128
6 Leclerc MC, Philippe H, Gueho E. Phylogeny of dennatophytes and dimorphic fungi based on large subunit ribosomal RNA sequence comparisons. J Med Vet Mycol 1994; 32: 331-341   DOI   ScienceOn
7 Makimura K, Tamura Y, Mochizuki T, Hasegawa A, Tajiri Y, Hanazawa R, Uchida K, Saito H, Yamaguchi H. Phylogenetic classification and species identification of dermatophyte strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 1999; 37: 920-924
8 Scott DW, Miller WH, Griffin CE. Dermatophytosis. In: Small animal dermatology, 6th ed. New York: WB Saunders. 2001: 339-347
9 Summerbell RC. What is the evolutionary and taxonomic status of asexual lineages in dermatophytes? Stud Mycol 2002; 47: 97-101
10 Takashio M. The Trichophyton mentagrophytes complex. In: Recent advances in medical and veterinary mycology, Iwata K, ed. Tokyo: University of Tokyo Press. 1977: 271-276
11 Davidson FD, Mackenzie DWR, Owen RJ. Deoxyribonucleic acid base compositions of dermatophytes. J Gen Microbial 1980; 118: 465-470
12 Nishio K, Kawasaki M, Ishizaki H. Phylogeny of the genera Trichophyton using mitochondrial DNA analysis. Mycopathologia 1992; 117: 127-132   DOI
13 Davidson FD, Mackenzie DWR. DNA homology studies in the taxonomy of dermatophytes. Sabouraudia 1984; 2: 117-123
14 Kane J, Summerbell R, Sigler L, Kraiden S, Land G. Laboratory handbook of dennatophytes. Belmont, USA: Star Publication. 1997
15 Faggi E, Pini G, Campisi E, Bertellini C, Difonzo E, Mancianti F. Application of PCR to distinguish common species of dermatophytes. J Clin Microbiol 2001; 39: 3382-3385   DOI
16 Lobuglio K, Pitt JI. Taylor JW. Phylogenetic analysis of two ribosomal DNA regions indicates multiple independent losses of a sexual Talaromyces state among asexual Penicillium species in subgenus Biverticillium. Mycologia 1993; 85: 592-604   DOI
17 Harmsen D, Schwinn A, Weig W, Brocher EB, Heesemann J. Phylogeny and dating of some pathogenic keratinophilic fungi using small subunit ribosomal RNA. J Med Vet Mycol 1995; 33: 299-303   DOI   ScienceOn