• Title/Summary/Keyword: mole cricket

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Novel Alkali-Tolerant GH10 Endo-${\beta}$-1,4-Xylanase with Broad Substrate Specificity from Microbacterium trichothecenolyticum HY-17, a Gut Bacterium of the Mole Cricket Gryllotalpa orientalis

  • Kim, Do Young;Shin, Dong-Ha;Jung, Sora;Kim, Hyangmi;Lee, Jong Suk;Cho, Han-Young;Bae, Kyung Sook;Sung, Chang-Keun;Rhee, Young Ha;Son, Kwang-Hee;Park, Ho-Yong
    • Journal of Microbiology and Biotechnology
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    • v.24 no.7
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    • pp.943-953
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    • 2014
  • The XylH gene (1,167-bp) encoding a novel hemicellulase (41,584 Da) was identified from the genome of Microbacterium trichothecenolyticum HY-17, a gastrointestinal bacterium of Gryllotalpa orientalis. The enzyme consisted of a single catalytic domain, which is 74% identical to that of an endo-${\beta}$-1,4-xylanase (GH10) from Isoptericola variabilis 225. Unlike other endo-${\beta}$-1,4-xylanases from invertebrate-symbiotic bacteria, rXylH was an alkali-tolerant multifunctional enzyme possessing endo-${\beta}$-1,4-xylanase activity together with ${\beta}$-1,3/${\beta}$-1,4-glucanase activity, which exhibited its highest xylanolytic activity at pH 9.0 and 60oC, and was relatively stable within a broad pH range of 5.0-10.0. The susceptibilities of different xylosebased polysaccharides to the XylH were assessed to be as follows: oat spelts xylan > beechwood xylan > birchwood xylan > wheat arabinoxylan. rXylH was also able to readily cleave p-nitrophenyl (pNP) cellobioside and pNP-xylopyranoside, but did not hydrolyze other pNP-sugar derivatives, xylobiose, or hexose-based materials. Enzymatic hydrolysis of birchwood xylan resulted in the product composition of xylobiose (71.2%) and xylotriose (28.8%) as end products.

Development of continuous indoor rearing technique of mole cricket, Gryllotalpa orientalis Burmeister (Orthoptera: Gryllotalpidae) (땅강아지(Gryllotalpa orientalis Burmeister)의 실내 누대사육 기술개발)

  • Park, Young-Kyu;Lee, Young-Bo
    • Journal of Sericultural and Entomological Science
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    • v.52 no.2
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    • pp.96-101
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    • 2014
  • The present study was conducted for two years to develop the indoor breeding system of mole cricket, Gryllotalpa orientalis Burmeister. In 2013, the total mean number of progenies was $430.{\pm}20.0$ ($mean{\pm}SE$); $15.3{\pm}25.8$ of 2nd-3rd instars, $28.8{\pm}24.0$ 3rd-5th and $2.4{\pm}2.7$ 6th-8th. In 2014, the rearing yielded similar result to that of 2013; $43.5{\pm}31.0$ in total with $25.8{\pm}25.6$ of 2nd-3rd instars, $23.6{\pm}21.3$ 3rd-5th and $2.9{\pm}2.3$ 6th-8th. The successful breeding rate using indoor cages was 46.9% in 2013 and 50.0% in 2014. In total 147 individuals were successfully reared in an outdoor rearing cage with 25 females released and 78 individuals in an indoor rearing cage with 100 females and 50 males released. 86.4% and 89.7% of the nymphs reared were collected within 25cm depth of the soil in the outdoor and indoor cage, respectively.

Mitochondrial DNA Sequence Variation of the Oriental Mole Cricket, Gryllotalpa orientalis (Orthoptera: Gryllotalpidae) in Korea

  • Kim, Ik-Soo;Cha, So-Young;Lee, Sun-Young;Kim, Seong-Ryul;Hwang, Jae-Sam;Li, Jianhong;Han, Yeon-Soo;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.2
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    • pp.107-112
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    • 2007
  • The mole crickets, Gryllotalpa, are insect pest distributed in the world. In Korea, G. orientalis was reported to occur, but previous ecological studies suggested the presence of two ecological types. To test this hypothesis, we sequenced a portion of mitochondrial (mt) genome from 48 G. orientali individuals collected over five Korean localities: Busan, Suwon, Okchon, Wonju, and Gangneung. From the sequence analysis, only two haplotypes were obtained, but the sequence divergence between the two haplotypes was 11 %, suggesting the presence of two distinct genetic groups in Korea. Although the population of Busan, Okchon, Wonju, and Gangneung was identified as a single haplotype, but that of Suwon was occupied by both hapotypes. Considering sequence divergence of other insect species occurring in Korea, the divergence estimate found between the two haplotypes seems to be too large to be considered as identical species. This result may suggest that the two differentiated haplotypes found in this study may reflect the previously reported two ecological types found in Suwon, Korea. To further understand the genetic divergence of the two phylogenetic groups, analysis of more variable regions of G. orientalis genome is required.

Seasonal Changes in Age Structure and Fecundity of the African Mole Cricket (Gryllotalpa africana) Population in Suwon, Korea (수원지방에서의 땅강아지 개체군 연령 분포의 계절적 변화와 산란수)

  • 김기황
    • Korean journal of applied entomology
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    • v.34 no.1
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    • pp.70-74
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    • 1995
  • A study on seasonal changes in age structure and fecundity of Gryllotalpa africana Palisot de Beauvois was conducted in Suwon from 1991 through 194. It seemed that most of newly hatched nymphs emerge to adults in September and October, so have a life cycle a year, and some overwinter as nymphs and emerge to adults from August, so have a life cycle tow years. The mean number of eggs per cell was $36.00\pm$2.84 for egg cells laid in the laboratory, and $47.68\pm$4.35 for egg cells collected in the field. Mean and range of the numbers of egg cells produced per female were $1.17\pm$0.19 and 0~3.

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Non-Chemical or Low-Chemical Control Measures against Key Insect Pests and Rats in the Ginseng Fields (인삼 포장에서의 해충 및 쥐의 비농약적 또는 저농약적 방제법)

  • Kim, Ki-Whang
    • Korean journal of applied entomology
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    • v.39 no.4
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    • pp.281-286
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    • 2000
  • Non-chemical or low-chemical control measures against key insect pests and rats in the ginseng field were studied from 1993 through 1999. Broadcasting chemicals beside the ginseng field in the oviposition period showed the same control effect on the adults of the Korean black chafer, Holotrichia diomphalia, as broadcasting at the ginseng ridge. Ginseng damage by adults of African mole cricket, Gryl1ota1pa africana, were reduced considerably by broadcasting chemicals beside the ginseng field. The larvae of wheat wireworm, Ectinus sericeus, were attracted effectively to potatoes in the ginseng field. Spreads of the mealybug, Pseudococcus comstocki, were very slow in the ginseng field, indicating that it is possible to eradicate the early colonies of Pseudococcus comstocki effectively. The rat repeller, Dekur $500S^{showed}$ a significant control effect of rats in the ginseng field.

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