• Title/Summary/Keyword: modified smear method

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Platelet Anti-Aggregating Plant Materials

  • YunChoi, Hye-Sook;Kim, Jae-Hoon;Kim, Sun-Ok;Lee, Jong-Ran
    • Korean Journal of Pharmacognosy
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    • v.17 no.2
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    • pp.161-167
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    • 1986
  • The smear method developed by Velaskar and Chitre was modified to allow the screening of plant extracts and/or fractions for platelet anti-aggregating activity. The modified smear method was also found suitable for massive screening of pure compounds. Sample fractions prepared from various plant extracts were examined for their effects against ADP, arachidonic acid (AA) or collagen induced platelet aggregations. Several solvent fractions of plant extracts including water fraction prepared from the methanol extract of Acanthopanax sp. was inhibitory against rat platelet aggregations. The activity guided treatments and fractionations of the water fraction from A. senticosus Max yielded two anti-platelet aggregatory substances, 3, 4-dihydroxybenzoic acid (I) and its artefact ethyl 3, 4-dihydroxybenzoate(II). The inhibitory activities of I and II against rat platelet aggregation were compared with that of aspirin, a known inhibitor of platelet aggregation. Discussions also included the results of the investigations on the structural activity relationships among the various dihydroxybenzoic acid derivatives against platelet aggregations induced by either one of ADP, AA or collagen.

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Screening of Inhibitors of Platelet Aggregation from Edible Plants (산채류로부터 혈소판응집 억제물질의 검색)

  • Yoon, Min-Ho;Lim, Chi-Hwan;Oh, Jin-Hwan;Lee, Jong-Chul;Choi, Woo-Young
    • Korean Journal of Agricultural Science
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    • v.24 no.2
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    • pp.267-274
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    • 1997
  • To select potential inhibitors of platelet aggregation from large numbers of crude plant extracts, the modified thin smear method for the anti-platelet aggregating activity using platelet rich plasma was further modified by direct observation under a light microscope without staining the smear. The activities determined by the method were coincided with those by the electrical impedence method using whole blood, when ADP or collagen was employed as the aggregating agent. Among 130 varieties of edible and herbal plants which collected from markets or experimental farms of agricultural research institutes, those showed the anti-platelet aggregating activities were selected by testing the crude methanol extracts: Aster scaber, Aster tataricus, Ligularia stenocephala, Platycodon glaucum Allium victorialis, Allium oderum, Moros bombycis, Portulaco oleracea, Aamthopanax sessiliflorus and Rosa davurica. However, some of them activated the platelet aggregation under the same assay conditions: Pimpinella brachycarpa, Hosta plantaginea, Capsella bursapastoris, Fagopyrm esculentum, Prunus mume, Rubus coreanus and Limaria japonica. In addition, those revealed the antioxidant activities were selected by measuring the abilities to scavenge superoxide anion radicals: Pteridium aquilinum, Aster scaber, Ligularia fischeri, Chrysanthemum zawadskii, Artemisia capipparis, Cirsium setidens, Commelina communis and Capsella bursapastoris among edible plants.

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A Behavior Ana1ysis of Clayey Foundation Improved with Pack Drain (Pack-Drain으로 개량된 점토지반의 거동해석)

  • 오재화;남기현;이문수;허재은;김영남
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.38 no.1
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    • pp.116-127
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    • 1996
  • This paper dealt with FEM analysis of foundation improved with pack drain. The theory on pack drain was scrutinized and observed values in the field were compared with numerical results. Work site of Kwangyang container pier was selected as a ease study in which measurement of settlement and pore water pressure was accurately carried out. Biot's consolidation equation was selected as governing One, coupled with modified Camclay model as constitutive one. Christian and Boehmer's numerical technique was adopted. Behavior of foundation with pack drain is not simple but very complicated. Discontinuity resulted from rigidity difference between adjacent materials, smear effect and complicated boundary conditions should be considered in the behavior analysis of foundation behavior. The results of numerical analysis were influenced by smear zone. In relevant to this effect, finite element analysis was carried out using the reduced horizontal coefficient of permeability in the smear zone; The numerical results were compared with observed values in surface settlement. including pore water pressure. However only lateral di5plaoement by numerical ana1Ysis was shown since its measurement was not performed in the field. The predication of settlement to be developed later can be effectively employed for the obtimization of construction. The predication of residual settlement using the data measured in the field was made by Hoshino, Asaoka and hyperbolic method. Among them, the hyperbolic method proved best one. Settlements accorded well between numsrical and observed values while pore pressure showed a slight difference. Lateral displacement showed largest values at constant distance from ground surface. The validation of foundation analysis improved with pack drain by computer program employed in this study selecting modified Cam-clay model was satisfactorily secured.

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연약지반 변형해석을 위한 다목적 Program개발

  • 박병기;정진섭
    • Proceedings of the Korean Geotechical Society Conference
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    • 1991.10a
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    • pp.362-375
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    • 1991
  • Background and Necessity of the study : For more than 20 years, the soil engineering reserach group of Chonnam National University has been performing the deformation analysis of soft clayey foundation, since the University is located near the south-western coast of Korean Peninsulla, along which tide reclamation works have been under proaressing. Associsted with the fact mentioned above, the researchers have been developing a computer program in order to carry out deformation analysis of soft foundation since early 1980. Case-studies : In this research, the Biot's equation was selected as the governing equation coupled with several constitutive models including original and modified Cam-clay models, elasto-viscoplastic model, Lade's model etc. The anisotropy of soi1 can be considered in this program. To validate the accuracy of the computer program developed a couple of case-studies were performed. These include the pilot banking, sand drain considering smear effect and compound foundation reinforced with sheet pile into soft foundation.i) The pilot banking Good results could be acquired by assuming banking load as the body force composed of finite element mesh rather than equivalent concentrated load.ii) The sand drain Due to smear, the delay of consolidation was remarkable at the early stsge. so safety for the failure of foundation should be checked for the initial step of consolidation. iii) The compound foundation Accurate results were obtained by introducing the joint element method for the soft foundation reinforced with sheet pile into soiㅣ.

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Consolidation Analysis for PVD Installed Soft Ground Using a Modified Theoretical Solution (변형된 이론해를 이용한 연직배수재 설치 지반의 압밀해석)

  • Hong, Sung-Jin;Kim, Dong-Hee;Kim, Yun-Tae;Kim, Hyung-Sub;Lee, Woo-Jin
    • Journal of the Korean Geotechnical Society
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    • v.28 no.1
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    • pp.41-53
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    • 2012
  • As the permeability of soil adjacent to the vertical drain has a decisive effect on the rate of consolidation, the permeability of smear zone governs the rate of radial consolidation of PVD installed soft ground. In this study, a method was suggested to analyze the radial consolidation, based on consolidation characteristics of remolded clay, and was used to evaluate the consolidation of soft clay layer in Busan Newport. The suggested method provides more reliable consolidation behaviors than the conventional approach, which is based on the consolidation characteristics of undisturbed clay. The suggested method is also observed to be relatively insensitive to the uncertainty of $k_h/k_s$. The comparison between the analysis and field measurement revealed that the suggested method provided a reliable prediction on the rate of consolidation of PVD installed Busan new port clay and that an appropriate extent of smear zone was evaluated as about $3d_w$ by back analysis.

Evaluation of Giarydia lumblia Detection Method in Stool Specimens Fixed with SAF Solution (SAF 고정변에서 람블편모충(Giardia lamblia)시스트 검출을 위한 검사방법의 평가)

  • Kim, Yeong-Chang;Kim, Jae-Jin;Lee, Geun-Tae
    • Parasites, Hosts and Diseases
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    • v.23 no.2
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    • pp.285-292
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    • 1985
  • The present study has been designed as a basic study on laboratory diagnosis of giardiasis and to demonstrate a more effective method for the detection of Giardia lamblia cyst with the inherent advantages of minimizing both the number of stool examinations required and the interval of stool collections for estimating the real state of prevalence in the shortest time possible. There were 3 subject groups of 75 children each currently residing in an orphanage in Gunsan city, Jeonbuk province from which stool specimens were collected every day, every other day, and every 3 days. The procedure is as follows: 1) resuspend the InKed sample after fixation with SAF solution 2) centrifuge the sediment for 1 min. at 2, 000 rpm after straining through gauze into a tube 3) divide the sediment into 3 parts and use them for direct fecal smear, formalin-ether concentration (MGL) and zinc sulfate ($ZnSO_4$) floatation techniques. The results are summarized as follows: 1. Overall infection rate after 10 trials showed a 60% positive indication. The positive rate among children under 4 years old was significantly higher than the rate in children over 4 years old. No significant difference in rate by sex was observed. 2. The results of eBaminations by direct fecal smear and MGL techniques appeared more accurate than that obtained by $ZnSO_4$ ftoatation method as indicated by a higher positive rate. Of all three methods concerned, combinations of two demonstrated a higher positive rate than that shown by any one alone. 3. In three consecutive examinations under varying conditions such as different days, the cyst detection rate by MGL technique indicated 83%. In 5 examinations under the same varying conditions, the indicated rate was 94%. 4. The interval of stool collection proved to be insignificant for the cyst detection rate. In conclusion, both MGL method and modified fecal direct smear can provide a good cyst detection rate of G. lamblia provided that more than 3 consecutive examinations of stool under varying conditions are carried out.

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Modus Operandi: Irrigation of the Modified Eloesser Flap in Heterogeneous Suppurative Lung Pathologies

  • Vishnu, Rajkamal;Rai, Guruprasad D.;Kamath, Ganesh Sevagur;Kumara, Vijaya
    • Journal of Chest Surgery
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    • v.54 no.2
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    • pp.137-142
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    • 2021
  • Background: Refractory empyemas with collapsed lung and persistent bronchopleural fistulas pose significant problems to thoracic surgeons and impose a substantial burden in terms of morbidity and mortality. The modified Eloesser flap procedure is a useful palliative option for clearing infections. Herein, we present our experiences with the modified Eloesser flap procedure in mixed suppurative lung pathologies with a new technique of irrigation for persistent infection. Methods: A retrospective review was carried out of 56 patients who underwent the modified Eloesser flap with continuous irrigation at Katurba Medical College. These patients had severe morbidities and were not suitable for major thoracic resection surgery, and electively underwent modified Eloesser flap surgery. Regular follow-up was done at 1, 3, 6, and 12 months. Patients with persistent infections were treated with our continuous irrigation technique. Results: The most important finding was that all patients with active sputum acid-fast bacilli-positive findings became sputum smear-negative during the first month of follow-up. Half (50%) of the patients had a patent stoma. Eleven patients had persistent infections, necessitating continuous irrigation. The infection was fully cleared after 1 month in 9 patients, while 2 patients required second irrigation and continued to receive follow-up. In the remaining 50% of the patients, the stoma closed completely, and the lung expanded fully. Conclusion: The modified Eloesser flap is a simple procedure. In suppurative pathologies, infections were well controlled and the general condition of the patients improved. Our continuous irrigation method showed promising results in patients with persistent purulent discharge.

Deformation Analysis of Soft Foundation with Vertical Drain Wells using the Interface Element Method -With Emphasis on Model Foundation and Actual Sand Drain Well Foundation- (접합요소에 의한 Vertical Drain Well 지반의 변형해석 - 모델지반과 실제 Sand Drain Well 지반을 중심으로 -)

  • Lee, Jean Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.4
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    • pp.227-237
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    • 1993
  • This paper dealt with numerical analysis of sand drain considering the smear effect around drain wells and discontinuous deformation behavior due to difference in rigidity between drain materials and adjacent clayey soils. Biot's equation was selected as governing equation coupled with MODCAM (Modified Cam-clay) model or EVP(Elasto-Viscoplastic) model as constitutive equation. The validity as well as the accuracy of the method developed by author was checked by comparing the proposed method with those by Siriwardane and Ghaboussi using joint element. The FEM analysis developed in this study was applied to both 2-dimensional model foundation and actual foundation. the result of which proved to be satisfactory.

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Lesson and proposal of revised equations from the Pan method application case for soft clay improvement (PBD 공법 시공사례를 통한 교훈 및 개선안 제안)

  • 유한구;조영묵;김종석;박정규
    • Proceedings of the Korean Geotechical Society Conference
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    • 2001.10a
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    • pp.147-158
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    • 2001
  • In general, two methods have been used to predict settlement of soft ground. One method is Terzaghi's one dimensional consolidation theory which gives time-settlement relationship using the standard consolidation test results. The other is forecasting method of ground settlement to be occured in the future using in-situ monitoring data. The above both methods have some defects in application manner or in itself especially in very deep and soft clayey ground. In view of the lessons and experiences of soft ground improvement projects, several techniques were proposed for more accurate theorectical calculation of consolidation settlement as follows ; ① Subdivision of soft ground, ② Consideration of secondary compression, ③ Using the modified compression index, etc. And also, revised hyperbolic fitting method was suggested to minimize the error of predicted future settlement. In addition, revised De-Beer equation of immediate settlement of loose sandy soil was proposed to overcome the tendency to show too small settlement calculation results by original De-Deer equation. And also, considering the various effects of settlement delay in the improved ground by vertical drains, time-settlement caculation equation(Onoue method) was revised to match the tendency of settlement delay by using the characteristics of discharge capacity decreases of vertical drain with time elapse by the pattern of hyperbolic equation.

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Egg Laying Capacity of Hap/orchis taichui (Digenea: Heterophyidae) in Humans

  • Sato, Megumi;Sanguankiat, Surapol;Pubampen, Somchit;Kusolsuk, Teera;Maipanich, Wanna;Waikagul, Jitra
    • Parasites, Hosts and Diseases
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    • v.47 no.3
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    • pp.315-318
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    • 2009
  • Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.