Parasites, Hosts and Diseases
- Volume 23 Issue 2
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- Pages.285-292
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- 1985
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Evaluation of Giarydia lumblia Detection Method in Stool Specimens Fixed with SAF Solution
SAF 고정변에서 람블편모충(Giardia lamblia)시스트 검출을 위한 검사방법의 평가
- Kim, Yeong-Chang (Department of Parasitology, Yonsei University College of Medicine) ;
- Kim, Jae-Jin (Department of Parasitology, Yonsei University College of Medicine) ;
- Lee, Geun-Tae (Department of Parasitology, Yonsei University College of Medicine)
- Published : 1985.12.01
Abstract
The present study has been designed as a basic study on laboratory diagnosis of giardiasis and to demonstrate a more effective method for the detection of Giardia lamblia cyst with the inherent advantages of minimizing both the number of stool examinations required and the interval of stool collections for estimating the real state of prevalence in the shortest time possible. There were 3 subject groups of 75 children each currently residing in an orphanage in Gunsan city, Jeonbuk province from which stool specimens were collected every day, every other day, and every 3 days. The procedure is as follows: 1) resuspend the InKed sample after fixation with SAF solution 2) centrifuge the sediment for 1 min. at 2, 000 rpm after straining through gauze into a tube 3) divide the sediment into 3 parts and use them for direct fecal smear, formalin-ether concentration (MGL) and zinc sulfate (
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