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Quantification of Protein and Amylose Contents by Near Infrared Reflectance Spectroscopy in Aroma Rice (근적외선 분광분석법을 이용한 향미벼의 아밀로스 및 단백질 정량분석)

  • Kim, Jeong-Soon;Song, Mi-Hee;Choi, Jae-Eul;Lee, Hee-Bong;Ahn, Sang-Nag
    • Korean Journal of Food Science and Technology
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    • v.40 no.6
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    • pp.603-610
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    • 2008
  • The principal objective of current study was to evaluate the potential of near infrared reflectance spectroscopy (NIRS) as a non-destructive method for the prediction of the amylose and protein contents of un-hulled and brown rice in broad-based calibration models. The average amylose and protein content of 75 rice accessions were 20.3% and 7.1%, respectively. Additionally, the range of amylose and protein content were 16.6-24.5% and 3.8-9.3%, respectively. In total, 79 rice germplasms representing a wide range of chemical characteristics, variable physical properties, and origins were scanned via NIRS for calibration and validation equations. The un-hulled and brown rice samples evidenced distinctly different patterns in a wavelength range from 1,440 nm to 2,400 nm in the original NIR spectra. The optimal performance calibration model could be obtained by MPLS (modified partial least squares) using the first derivative method (1:4:4:1) for un-hulled rice and the second derivative method (2:4:4:1) for brown rice. The correlation coefficients $(r^2)$ and standard error of calibration (SEC) of protein and amylose contents for the un-hulled rice were 0.86, 2.48, and 0.84, 1.13, respectively. The $r^2$ and SEC of protein and amylose content for brown rice were 0.95, 1.09 and 0.94, 0.42, respectively. The results of this study suggest that the NIRS technique could be utilized as a routine procedure for the quantification of protein and amylose contents in large accessions of un-hulled rice germplasms.

Experiments on the stability of the spatial autocorrelation method (SPAC) and linear array methods and on the imaginary part of the SPAC coefficients as an indicator of data quality (공간자기상관법 (SPAC)의 안정성과 선형 배열법과 자료 품질 지시자로 활용되는 SPAC 계수의 허수 성분에 대한 실험)

  • Margaryan, Sos;Yokoi, Toshiaki;Hayashi, Koichi
    • Geophysics and Geophysical Exploration
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    • v.12 no.1
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    • pp.121-131
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    • 2009
  • In recent years, microtremor array observations have been used for estimation of shear-wave velocity structures. One of the methods is the conventional spatial autocorrelation (SPAC) method, which requires simultaneous recording at least with three or four sensors. Modified SPAC methods such as 2sSPAC, and linear array methods, allow estimating shear-wave structures by using only two sensors, but suffer from instability of the spatial autocorrelation coefficient for frequency ranges higher than 1.0 Hz. Based on microtremor measurements from four different size triangular arrays and four same-size triangular and linear arrays, we have demonstrated the stability of SPAC coefficient for the frequency range from 2 to 4 or 5 Hz. The phase velocities, obtained by fitting the SPAC coefficients to the Bessel function, are also consistent up to the frequency 5 Hz. All data were processed by the SPAC method, with the exception of the spatial averaging for the linear array cases. The arrays were deployed sequentially at different times, near a site having existing Parallel Seismic (PS) borehole logging data. We also used the imaginary part of the SPAC coefficients as a data-quality indicator. Based on perturbations of the autocorrelation spectrum (and in some cases on visual examination of the record waveforms) we divided data into so-called 'reliable' and 'unreliable' categories. We then calculated the imaginary part of the SPAC spectrum for 'reliable', 'unreliable', and complete (i.e. 'reliable' and 'unreliable' datasets combined) datasets for each array, and compared the results. In the case of insufficient azimuthal distribution of the stations (the linear array) the imaginary curve shows some instability and can therefore be regarded as an indicator of insufficient spatial averaging. However, in the case of low coherency of the wavefield the imaginary curve does not show any significant instability.

Improvement of Meju Preparation Method for the Production of Korean Traditional kanjang (Soy Sauce) (한국 재래식 간장의 품질 향상을 위한 메주 제조법 개선)

  • Im, Moo-Hyeog;Choi, Jong-Dong;Chung, Hyun-Chae;Lee, Seon-Ho;Lee, Coon-Woo;Choi, Cheong;Choi, Kwang-Soo
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.608-614
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    • 1998
  • In order to establish the scientific foundations for the production of Korean traditional kanjang (soy sauce) in a semi-pilot scale, meju preparation and kanjang mashing methods were investigated. Two types of meju, one that was prepared by conventional method (CM-meju) and the other that prepared by modified conventional method (MCM-meju), were made and compared their characteristics. The former made of cooked and crushed soybean with a brick shape was fermented at $15{\sim}20^{\circ}C\;and\;40{\sim}50%$ relative humidity (RH) for 30 days in koji room and the latter was fermented at $25{\sim}30^{\circ}C\;and\;80{\sim}90%$ RH seemingly being optimum for the growth of meju-organisms for the same period. The quality of MCM-meju as a raw material for the kanjang preparation was considered to be superior to that of CM-meju as the higher soluble nitrogen and total free amino acids content, the major factor for the evaluation of the quality of meju, were found in the former although the higher total nitrogen content and lower % weight loss were observed in the latter during meju preparation process. The quality of MCM-kanjang with higher total nitrogen, free amino acids, free sugars and the lower residual nitrogen content in cake after separation of kanjang was also found to be superior to that of CM-kanjang in sensory evaluation results.

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Method Development for Determination of Multi-Mycotoxins in Chicken Liver and Kidney Tissues by LC-MS/MS (LC-MS/MS를 이용한 닭 간과 신장 중 곰팡이 독소 6종 동시분석법 개발)

  • Kim, Soohee;Kim, Kwang-Nam;Kim, Hyobi;Song, Jae-Young;Park, Sung-Won
    • Korean Journal of Poultry Science
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    • v.43 no.2
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    • pp.111-118
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    • 2016
  • Mycotoxins are secondary metabolites produced by molds, such as Aspergillus, Fusarium and Penicillium, that have adverse effects on animals and humans. Aflatoxin, ochratoxin, zearalenone, fumonisin and deoxynivalenol are the mycotoxins of greatest agro-economic importance and cause acute disease called mycotoxicoses. Mycotoxicosis in poultry birds results in decreased meat/egg production, immunosuppressant, and hepatotoxicosis. Some of toxins or their metabolites may be retained in animal or human tissues and induce health problems. This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of mycotoxins, such as aflatoxin $B_1$, aflatoxin $M_1$, ochratoxin A, zearalenone, fumonisin B and deoxynivalenol, in chicken liver and kidney tissues. The mycotoxins were extracted and purified using modified QUECHERS methods, separated by LC and detected by an electrospray ionisation interface (ESI) and tandem MS. Good precision and linearity were observed for most of six mycotoxins. The recovery test for each mycotoxin in liver and kidney tissues mostly indicated good average recovery rates between 80.94% and 98.10% and the coefficient of variation mostly under 13.78%, except for aflatoxin $M_1$ and fumonisin $B_1$. The limit of detection (LOD) for six mycotoxins was $7.6{\sim}145.79{\mu}g/kg$ in liver tissues and $6.07{\sim}197.20{\mu}g/kg$ in kidney tissues. The quantification limits (LOQ) for 6 mycotoxins were in the range $23.04{\sim}441.78{\mu}g/kg$ in liver tissues and $18.40{\sim}597.59{\mu}g/kg$ in kidney tissues, respectively. The developed multi-mycotoxin method in this study permits simultaneous, simple, and rapid determination of several co-existing mycotoxins in chicken liver and kidney tissues.

Increase of Tc-99m RBC SPECT Sensitivity for Small Liver Hemangioma using Ordered Subset Expectation Maximization Technique (Tc-99m RBC SPECT에서 Ordered Subset Expectation Maximization 기법을 이용한 작은 간 혈관종 진단 예민도의 향상)

  • Jeon, Tae-Joo;Bong, Jung-Kyun;Kim, Hee-Joung;Kim, Myung-Jin;Lee, Jong-Doo
    • The Korean Journal of Nuclear Medicine
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    • v.36 no.6
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    • pp.344-356
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    • 2002
  • Purpose: RBC blood pool SPECT has been used to diagnose focal liver lesion such as hemangioma owing to its high specificity. However, low spatial resolution is a major limitation of this modality. Recently, ordered subset expectation maximization (OSEM) has been introduced to obtain tomographic images for clinical application. We compared this new modified iterative reconstruction method, OSEM with conventional filtered back projection (FBP) in imaging of liver hemangioma. Materials and Methods: Sixty four projection data were acquired using dual head gamma camera in 28 lesions of 24 patients with cavernous hemangioma of liver and these raw data were transferred to LINUX based personal computer. After the replacement of header file as interfile, OSEM was performed under various conditions of subsets (1,2,4,8,16, and 32) and iteration numbers (1,2,4,8, and 16) to obtain the best setting for liver imaging. The best condition for imaging in our investigation was considered to be 4 iterations and 16 subsets. After then, all the images were processed by both FBP and OSEM. Three experts reviewed these images without any information. Results: According to blind review of 28 lesions, OSEM images revealed at least same or better image quality than those of FBP in nearly all cases. Although there showed no significant difference in detection of large lesions more than 3 cm, 5 lesions with 1.5 to 3 cm in diameter were detected by OSEM only. However, both techniques failed to depict 4 cases of small lesions less than 1.5 cm. Conclusion: OSEM revealed better contrast and define in depiction of liver hemangioma as well as higher sensitivity in detection of small lesions. Furthermore this reconstruction method dose not require high performance computer system or long reconstruction time, therefore OSEM is supposed to be good method that can be applied to RBC blood pool SPECT for the diagnosis of liver hemangioma.

Derivation of Green Coverage Ratio Based on Deep Learning Using MAV and UAV Aerial Images (유·무인 항공영상을 이용한 심층학습 기반 녹피율 산정)

  • Han, Seungyeon;Lee, Impyeong
    • Korean Journal of Remote Sensing
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    • v.37 no.6_1
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    • pp.1757-1766
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    • 2021
  • The green coverage ratio is the ratio of the land area to green coverage area, and it is used as a practical urban greening index. The green coverage ratio is calculated based on the land cover map, but low spatial resolution and inconsistent production cycle of land cover map make it difficult to calculate the correct green coverage area and analyze the precise green coverage. Therefore, this study proposes a new method to calculate green coverage area using aerial images and deep neural networks. Green coverage ratio can be quickly calculated using manned aerial images acquired by local governments, but precise analysis is difficult because components of image such as acquisition date, resolution, and sensors cannot be selected and modified. This limitation can be supplemented by using an unmanned aerial vehicle that can mount various sensors and acquire high-resolution images due to low-altitude flight. In this study, we proposed a method to calculate green coverage ratio from manned or unmanned aerial images, and experimentally verified the proposed method. Aerial images enable precise analysis by high resolution and relatively constant cycles, and deep learning can automatically detect green coverage area in aerial images. Local governments acquire manned aerial images for various purposes every year and we can utilize them to calculate green coverage ratio quickly. However, acquired manned aerial images may be difficult to accurately analyze because details such as acquisition date, resolution, and sensors cannot be selected. These limitations can be supplemented by using unmanned aerial vehicles that can mount various sensors and acquire high-resolution images due to low-altitude flight. Accordingly, the green coverage ratio was calculated from the two aerial images, and as a result, it could be calculated with high accuracy from all green types. However, the green coverage ratio calculated from manned aerial images had limitations in complex environments. The unmanned aerial images used to compensate for this were able to calculate a high accuracy of green coverage ratio even in complex environments, and more precise green area detection was possible through additional band images. In the future, it is expected that the rust rate can be calculated effectively by using the newly acquired unmanned aerial imagery supplementary to the existing manned aerial imagery.

Dental Hygienist-Led Dental Hygiene Process of Care for Self-Support Program Participants in Gangneung (강릉시 자활근로사업 참여자 대상 치위생 과정 사례보고)

  • Yoo, Sang-Hee;Kwak, Seon-Hui;Lee, Sue-Hyang;Song, Ga-In;Bae, Soo-Myoung;Shin, Sun-Jung;Shin, Bo-Mi
    • Journal of dental hygiene science
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    • v.18 no.6
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    • pp.327-339
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    • 2018
  • This study aimed to provide basic data for establishing the clinical basis for dental hygienist-led dental hygiene process of care by identifying multiple risk factors for self-support program participants in Gangneung city; we also compared oral health status and behavioral changes through customized oral health care. Four dental hygienists who were evaluated for degree of conformity provided dental hygiene process of care to eight self-support program participants who were selected as having an oral health risk among people in the self-support center. The clinical indicators measured during dental hygiene assessment and evaluation and behavioral changes due to dental hygiene intervention were compared and analyzed. With respect to clinical indicators, at the time of probe, the retention rate of patients with gingival bleeding decreased from 61.4% to 14.7% after intervention (p=0.004). Furthermore, the retention rate of patients with a periodontal pocket >4 mm decreased from 15.6% to 5.8% (p=0.001). The average modified O'Leary index of the patients improved from 23 to 40 (p=0.002). Previously, all eight subjects used the vertical or horizontal method of brushing; after dental hygiene care interventions regarding method and frequency of toothbrushing, use of oral care products, and individual interventions, they started using the rolling or Bass method of toothbrushing. Four of eight subjects reported using interdental toothbrushes after intervention. As a result of applying the change model to the transtheoretical behavior change of the subject, the result of strengthening the health behavior was confirmed. For promotion of oral health by the prevention-centered incremental oral health care system, dental hygienist-led dental hygiene management and maintenance is essential. It is thought that continuous research, such as for feasibility evaluation, cost benefit analysis, and preparation of legal systems, is needed to establish and activate dental hygiene management.

Analysis of Start-up Sustainability Factors Based on ERIS Model: Focusing on the Organization Resilience (ERIS모델 기반 창업지속요인 분석: 조직 리질리언스를 중심으로)

  • Kim, InSook;Yang, Ji Hee
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.5
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    • pp.15-29
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    • 2021
  • This study is based on ERIS model for start-up performance, and aims to derive the main reason for start-up sustainability centered on organizational resilience. To this end, systematic literature examination and modified Delphi method were used to investigate start-up sustainability factors based on ERIS Model focused on organizational resilience. The results showed that ERIS model-based entrepreneurial continuity factors were divided into four categories: entrepreneur, resource, industrial environment, strategy, subdivision 8 and detailed factors 54. In addition, the ERIS model-based continuity factors were structured around organizational resilience, and the continuity factors were structured according to ERIS model under five categories: leadership, culture, people, system and environment. The results of this study are as follows. First of all, the results of existing research and analysis show that the concept of successful start-up and sustainability of start-up are used in various fields. Second, it is confirmed that there are common factors of influence on start-up performance and start-up sustainability based on ERIS model. Third, Delphi method's results showed that the general characteristics of entrepreneurs, such as academic background, education level, gender, age, and business experience did not affect the sustainability of entrepreneurship. This study is significant in that it is based on ERIS model focused on organization resilience, and ERIS-R, which integrates Strategy into System and Organization resilience into R in the field of gradually expanding start-up development and support. It is expected that the results of this study will improve the sustainability of start-up that can predict, prevent, and overcome various crises at any time.

Isolation and Physicochemical Properties of Rice Starch from Rice Flour using Protease (단백질분해효소에 의한 쌀가루로부터 쌀전분의 분리 및 물리화학적 특성)

  • Kim, ReeJae;Oh, Jiwon;Kim, Hyun-Seok
    • Food Engineering Progress
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    • v.23 no.3
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    • pp.193-199
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    • 2019
  • This study aimed to investigate the impact of protease treatments on the yield of rice starch (RST) from frozen rice flours, and to compare the physicochemical properties of RST by alkaline steeping (control) and enzymatic isolation (E-RST) methods. Although the yield of E-RST, prepared according to conditions designed by the modified 23 complete factorial design, was lower than the control, the opposite trends were observed in its purity. E-RST (RST1, isolated for 8 h at 15℃ with 0.5% protease; RST2, isolated for 24 h at 15℃ with 1.5% protease; RST3, isolated for 24 h at 15℃ with 0.5% protease) with the yields above 50% were selected. Amylose contents did not significantly differ for the control and RST2. Relative to the control, solubilities were higher for all E-RST, but swelling power did not significantly differ for E-RST except for RST1. Although all E-RST revealed higher gelatinization temperatures than the control, the reversed trends were found in the gelatinization enthalpy. The pasting viscosities of all E-RST were lower than those of the control. Consequently, the enzymatic isolation method using protease would be a more time-saving and eco-friendly way of preparing RST than the alkaline steeping method, even though its characteristics are different.

Approach to the Extraction Method on Minerals of Ginseng Extract (추출조건(抽出條件)에 따른 인삼(人蔘)엑기스의 무기성분정량(無機成分定量)에 관(關)한 연구(硏究))

  • Cho, Han-Ok;Lee, Joong-Hwa;Cho, Sung-Hwan;Choi, Young-Hee
    • Korean Journal of Food Science and Technology
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    • v.8 no.2
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    • pp.95-106
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    • 1976
  • In order to investigate chemical components and mineral of ginseng cultivated in Korea and to establish an appropriate extraction method, the present work was carried out with Raw ginseng(SC), White ginseng(SB) and Ginseng tail(SA). The results determined could be summarized as follows : 1. Among the proximate components, moisture content of SC, SB and SA were 66.37%, 12.61% and 12.20% respectively. The content of crude ash in SA was the highest value of three kinds of ginseng root: SA 6.04%, SB 3.52% and SC 1.56%. The crude protein of Dried ginseng root(SA and SB) was about 12-14%, which was more than two times compared with that of SC(6.30%) The content of pure protein seemed to be in similar tendency with that of crude protein in three kinds of ginseng root: 2.26% in SC, 5.94% in SB and 5.76% in SA. There was no significant difference in the content of fat among the kinds of ginseng root. $(1.1{\sim}2.5%)$ 2. The highest Ginseng extract was obtained by use of Continuous extractor which is a modified Soxhlet apparatus for 60 hours extraction with 60-80% ethanol. 3. Ginseng and the above-mentioned ginseng extract (Ginseng tail extract: SAE, White Ginseng extract : SBE, Raw Ginseng extract: SCE) were analyzed by volumetric method for the determination of Chlorine and Calcium, by colorimetric method for that of Iron and Phosphorus, by Atomic Absorption Spectrophotometer for that of Zinc, Copper and Manganese. The results were as follows : 1. The content of phosphorus in SA, SB and SC were 1.818%, 1.362%, 0.713% respectively and phosphorus content in three kinds of extract were in low level (SAE: 0.03%, SBE: 0.063%, SCE: 0.036%) 2. In the Calcium content, SA, SB and SC were 0.147%, 0.238%, 0.126% and the Calcium contents of Ginseng extracts were 0.023%, 0.011% and 0.016%. The extraction ratio of Calcium from SA was the highest value (15.6%), while that in the case of SB was 4.6%. 3. The Chlorine content of SA was 0.11%, this was slightly higher than others(SB: 0.07%, SC: 0.09%) and extraction ratio of SA and SB were 36.4%, 67.1% while that of SC was 84.4%. 4. The Iron content of SA, SB and SC were 125ppm, 32.5ppm and 20ppm but extraction ratio was extremely low (SAE: 1.33%, SBE: 0.83%, SCE: 1.08%), 5. The Manganese content of SA, SB and SC were 62.5ppm, 25.0ppm and 5.0ppm respectively but the Manganese content of extract could not determined, Copper content of SA, SB and SC were 15.0ppm, 20.0ppm and those of extract were 7.5ppm, 6.5ppm, 4.5ppm while those of extraction ratio were 50%, 32.5% and 90% respectively, Zinc was abundant in Ginseng compared with other herbs, (SA: 45.5ppm, SB: 27.5ppm and SC: 5.5ppm) and the extracted amount were 4.5ppm, 1.25ppm 1.50ppm respectively.

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