• Journal of Pharmaceutical Investigation
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• v.36 no.2
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• pp.115-121
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• 2006

The aim of this study was to develop new protein/peptide depot system instead of poly(DL-lactic acid-coglycolic acid) (PLGA) microspheres. Pullulan was chemically modified by the addition of acetic anhydride (pullulan acetate; PA) and then investigated as new depot system for protein/peptide delivery. PA microspheres (PAM) with lysozyme as a model protein were prepared by w/o/w double emulsion method. The microspheres had a mean size of 10-50 mm with a spherical shape. The size distributions reduced with increasing the degree of acetylation. The loading efficiency of lysozyme was also increased. Lysozyme aggregation behavior in the microsphere was monitored to estimate the change of protein stability during preparation step. The ratios of protein aggregation in PAMs are lower than that of PLGA microsphere, in particular, PA 5 showed lowest as about 16%. The result indicated that the increase of acetylation suppressed the aggregation of protein. The release profiles of lysozyme from PAMs were significantly different. High acetylation effectively improved lysozyme release kinetics by reducing initial burst release and extending continuous release over a period of time. To check the effect of preservation for structural stability of lysozyme, the activity of lysozyme released from PA 5 was also observed. The activity of lysozyme was maintained almost 100% for 25 day. Therefore, PAM may become to a useful carrier for delivery of protein/peptide drugs, if it will be supported by biocompatibility and biodegradability results.

• Structural Engineering and Mechanics
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• v.67 no.1
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• pp.21-31
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• 2018

In this work, the dynamic stability of carbon nanotubes (CNTs) reinforced composite pipes conveying pulsating fluid flow is investigated. The pipe is surrounded by viscoelastic medium containing spring, shear and damper coefficients. Due to the existence of CNTs, the pipe is subjected to a 2D magnetic field. The radial induced force by pulsating fluid is obtained by the Navier-Stokes equation. The equivalent characteristics of the nanocomposite structure are calculated using Mori-Tanaka model. Based on first order shear deformation theory (FSDT) or Mindlin theory, energy method and Hamilton's principle, the motion equations are derived. Using harmonic differential quadrature method (HDQM) in conjunction with the Bolotin's method, the dynamic instability region (DIR) of the system is calculated. The effects of different parameters such as volume fraction of CNTs, magnetic field, boundary conditions, fluid velocity and geometrical parameters of pipe are shown on the DIR of the structure. Results show that with increasing volume fraction of CNTs, the DIR shifts to the higher frequency. In addition, the DIR of the structure will be happened at lower excitation frequencies with increasing the fluid velocity.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.05a
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• pp.121-127
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• 2003

Wave overtopping is one of the most important hydraulic responses of breakwater because it significantly affects its functional efficiency, the safety of transit and mooring on the rear side, wave transmission in the sheltered area, rear side armor stones and to some extent, the structural safety itself. In this study, hydraulic model tests has been carried out to investigate the influence of berm's size on overtopping rate by maximum overtopping rate and mean overtopping rate. The hydrodynamic characteristics of berm breakwater by the overtopping rate can be summarized as follows: 1. It is better to use maximum overtopping rate than to use mean overtopping rate for design of coastal structures in the point of view of stability. 2. When construct berm to decrease energy of waves that it was needed to make breaking conditions of wave on the berm. 3. Under the relative length of berm was over 0.13 overtopping rate was significantly decreased. 4. Overtopping rate affected significantly by the relative length of yhe berm than height of the berm.

• Structural Engineering and Mechanics
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• v.19 no.4
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• pp.401-411
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• 2005

The influence of cracks on the elastic deflection and ultimate bearing capacity of eccentric thin-walled columns with both ends pinned was studied in this paper. First, a method was developed and applied to determine the elastic deflection of the eccentric thin-walled columns containing some model-I cracks. A trigonometric series solution of the elastic deflection equation was obtained by the Rayleigh-Ritz energy method. Compared with the solution presented in Okamura (1981), this solution meets the needs of compatibility of deformation and is useful for thin-walled columns. Second, a two-criteria approach to determine the stability factor ${\varphi}$ has been suggested and its analytical formula has been derived. Finally, as an example, box columns with a center through-wall crack were analyzed and calculated. The effects of cracks on both the maximum deflection and the stability coefficient ${\varphi}$ for various crack lengths or eccentricities were illustrated and discussed. The analytical and numerical results of tests on the columns show that the deflection increment caused by the cracks increases with increased crack length or eccentricity, and the critical transition crack length from yielding failure to fracture failure ${\xi}_c$ is found to decrease with an increase of the slenderness ratio or eccentricity.

• Bulletin of the Korean Chemical Society
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• v.33 no.8
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• pp.2508-2512
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• 2012

Cold shock proteins (CSPs) are a family of proteins induced at low temperatures. CSPs bind to single-stranded nucleic acids through the ribonucleoprotein 1 and 2 (RNP 1 and 2) binding motifs. CSPs play an essential role in cold adaptation by regulating transcription and translation via molecular chaperones. The solution nuclear magnetic resonance (NMR) or X-ray crystal structures of several CSPs from various microorganisms have been determined, but structural characteristics of psychrophilic CSPs have not been studied. Therefore, we optimized the purification process to obtain highly pure Lm-Csp and determined the three-dimensional structure model of Lm-Csp by comparative homology modeling using MODELLER on the basis of the solution NMR structure of Bs-CspB. Lm-Csp consists of a ${\beta}$-barrel structure, which includes antiparallel ${\beta}$ strands (G4-N10, F15-I18, V26-H29, A46-D50, and P58-Q64). The template protein, Bs-CspB, shares a similar ${\beta}$ sheet structure and an identical chain fold to Lm-Csp. However, the sheets in Lm-Csp were much shorter than those of Bs-CspB. The Lm-Csp side chains, E2 and R20 form a salt bridge, thus, stabilizing the Lm-Csp structure. To evaluate the contribution of this ionic interaction as well as that of the hydrophobic patch on protein stability, we investigated the secondary structures of wild type and mutant protein (W8, F15, and R20) of Lm-Csp using circular dichroism (CD) spectroscopy. The results showed that solvent-exposed aromatic side chains as well as residues participating in ionic interactions are very important for structural stability. Further studies on the three-dimensional structure and dynamics of Lm-Csp using NMR spectroscopy are required.

• Journal of Aerospace System Engineering
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• v.16 no.5
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• pp.78-85
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• 2022

Multicopter-type unmanned aerial vehicles (UAV) are increasingly for cargo transportation to mountainous and island regions, image information acquisition in disaster areas, and emergency rescue transport. In order to successfully perform these tasks, the aircraft structure must be able to safely support the loads induced by flight conditions while ensuring the vibration and aeroelastic stability of the prop-rotor. This study introduced a structural analysis model of a 40kg payload multicopter with an engine-generator hybrid power system. The deformation and stress distribution are investigated depending on the load conditions. In addition, the vibration characteristics and aeroelastic stability of the prop-rotor were also presented to flight speed and aircraft pitch angle. The maximum thrust generated by the prop-rotor and the landing load applied to the multicopter under normal and emergency landing conditions were reviewed., It confirmed that the structure could support without failure. In addition, it confirmed that the damping characteristics of each primary locate in the constant region according to the aircraft's flight speed and the prop-rotors rotating speed.

• Geomechanics and Engineering
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• v.22 no.4
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• pp.291-303
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• 2020

The deformation of the rock surrounding a tunnel manifests due to the stress redistribution within the surrounding rock. By observing the deformation of the surrounding rock, we can not only determine the stability of the surrounding rock and supporting structure but also predict the future state of the surrounding rock. In this paper, we used grey system theory to analyse the factors that affect the deformation of the rock surrounding a tunnel. The results show that the 5 main influencing factors are longitudinal wave velocity, tunnel burial depth, groundwater development, surrounding rock support type and construction management level. Furthermore, we used seismic prospecting data, preliminary survey data and excavated section monitoring data to establish a neural network learning model to predict the total amount of deformation of the surrounding rock during tunnel collapse. Subsequently, the probability of a change in deformation in each predicted section was obtained by using a Bayesian method for detecting change points. Finally, through an analysis of the distribution of the change probability and a comparison with the actual situation, we deduced the survey mark at which collapse would most likely occur. Surface collapse suddenly occurred when the tunnel was excavated to this predicted distance. This work further proved that the Bayesian method can accurately detect change points for risk evaluation, enhancing the accuracy of tunnel collapse forecasting. This research provides a reference and a guide for future research on the probability analysis of tunnel collapse.

• Structural Engineering and Mechanics
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• v.39 no.4
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• pp.465-498
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• 2011

Concrete structures are generally cracked in flexural tension at working loads. Concrete beams with asymmetric section details and crack patterns exhibit different flexural rigidity depending upon the sense of the applied flexural moment. In this paper, three different models, having the same natural period, of such SDOF bilinear dynamical systems have been proposed. The Model-I and Model-II have constant damping coefficient, but the latter is characterized by two stiffness coefficients depending upon the sense of vibration amplitude. The Model-III, additionally, has two damping coefficients as well. In this paper, the dynamical response of Model-III to sinusoidal loading has been investigated and compared with that of Model-II studied earlier. It has been found that Model-III exhibits regular and irregular sub-harmonics, jump phenomena and strong sensitivity to initial conditions, forcing frequency, system period as well as the sense of peak sinusoidal force. The constant sustained load has been found to affect the natural period of the dynamical system. The predictions of Model-I have been compared with those of the approximate linear model adopted in present practice. The behaviour exhibited by different models of the SDOF cracked elastic concrete structures under working loads and the theoretical and practical implications of the approach followed have been critically evaluated.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.7
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• pp.1376-1383
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• 2002

Seismic qualification of the Air Cleaning Units for nuclear power plant Ulchin 5&6 has been performed with the guideline of ASME Section III and IEEE 344 code. By using the structural and geometrical similarity analysis, the three models to be analyzed are condensed into a single model and, at the same time, the excitation forces and other operating loads for each model are encompassed with respect to different loading conditions. As the fundamental frequencies of the structure are found to be less than 33Hz, which is the upper frequency limit of the seismic load, response spectrum analysis using ANSYS is performed in order to combine the modal stresses within the frequency limit. In order to confirm the structural and electric stability of the major components, modal analysis theory is adopted to derive the required response spectrum at the component locations. As the all combined stresses obtained from the above procedures are less than allowable stresses and no mechanical or electrical failures are found from the seismic testing, the authors confirm the safety of the nuclear equipments Air Cleaning Units studied in this paper.

• Proceedings of the KSME Conference
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• 2001.06b
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• pp.404-409
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• 2001

Seismic qualification of the Air Cleaning Units for nuclear power plant Ulchin 5&6 has been performed with the guideline of ASME Section III and IEEE 344 code. By using the structural and geometrical similarity analysis, the three models to be analyzed is condensed into a single model and, at the same time, the excitation forces and other operating loads for each model are encompassed with respect to different loading conditions. As the fundamental frequencies of the structure are found to be less than 33Hz, which is the upper frequency limit of the seismic load, response spectrum analysis using ANSYS is performed in order to combine the modal stresses within the frequency limit. In order to confirm the structural and electric stability of the major components, modal analysis theory is adopted to derive the required response spectrum at the component locations. As the all combined stresses obtained from the above procedures are less than allowable stresses and no mechanical or electrical failures are found from the seismic testing, the authors confirm the safety of the nuclear equipments Air Cleaning Units studied in this paper.