• Title/Summary/Keyword: mocR

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Analysis of Trans-Acting Elements for Regulation of moc Operons of pTi15955 in Agrobacterium tumefaciens

  • Jung, Won-Hee;Baek, Chang-Ho;Lee, Jeong-Kug;Kim, Kun-Soo
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.637-645
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    • 1999
  • Two putative regulator genes, mocR and mocS, of the moc (mannityl opine catabolism) operons in pTi15955 of the octopine-/mannityl opine-type Agrobacterium tumefaciens strain 15955, were tested for their possible roles as repressors in the moc operons. The regions upstream of macC and mocD, the first structural genes in the two divergently oriented moc operons, were transcriptionally fused into the promoterless lacZ reporter gene. Each of the lacZ-fusions was introduced into Agrobacterium strain UIA5, a Ti plasmid-cured derivative, harboring either a mocR or a mocS clone. The resulting strains were grown in media containing various sugar sources, and the $\beta$-galactosidase activities were quantitatively measured. The results suggested that MocR repressed the expression of macC and macD. The expression of the fused $\beta$-galactosidase was not induced by mannopine (MOP) or possible catabolic intermediates of the opine, e.g. santhopine (SOP), glucose, mannose, or glutamine. However, the repression was significantly relieved by the supplementation of MOP and the concomitant introduction of the agcA gene encoding MOP cyclase that catalyzes the lactonization of MOP to agropine (AGR). These results suggested that AGR, rather than MOP or the other catabolic intermediates, is the inducer for the expression of the operon. On the contrary to previous report showing that the induction levels of macC and macD were lowered by the supplementation of inorganic nitrogen in media, the expression of these genes was not affected by the level of nitrogen in our reporter system. MocS did not strongly repress the expressions of macC and mocD. It is possible that MocS may be involved in the regulation of the operons present downstream of the moc operon, which are responsible for the utilization of mannopinic acid and agropinic acid.

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Effect of Graded Levels of Mustard Oil Cake Supplementation on Intake, Nutrient Digestibility, Microbial N Yield of Adult Cannulated Native (Bos Indicus) Bulls Fed Rice Straw

  • Chowdhury, S.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.5
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    • pp.715-722
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    • 1999
  • On a urea-molasses-straw (3:15:82, UMS) based diet, effect of graded levels of mustard oil cake (MOC) supplementation on the performances of native bulls has been studied. Four cannulated adult Bos indicus bulls of $415({\pm}44.6)kg$ live weight and 80 months old, were given daily either of 0, 200, 400 or 800 g of MOC in four periods in a $4{\times}4$ latin square design. Besides, each animal also received 200 g of each of molasses and wheat bran and a mineral mixture. For unit (1 g) increase in MOC intake, total DM intake increased by 0.8 g/d ($r^2=0.88$) but no change in the straw DM intake. With the increasing levels of MOC, crude protein (CP) digestibility increased exponentially with an asymptotic value of 72%. However, MOC level had no effect on the digestibilities of DM, OM and ADF. Similarly, rumen degradability of rice straw was also not affected by the level of dietary MOC, and mean straw DM degradabilities were 15, 21. 28, 37, 47 and 51% at 8, 16, 24, 48, 72 and 96 hours of incubation respectively. Microbial N yield per kg digestible organic matter apparently fermented in the rumen were 7.46, 8.77, 6.88 and 5.96 g respectively for 0, 200, 400 or 800 g of dietary MOC. For each gram increase in dietary MOC, N intake and N balance increased by 0.054 g/d ($r^2=0.998$) and $0.59mg\;N/kg\;W^{0.75}/d$ ($r^2=0.99$) respectively. Nitrogen balance was estimated to be attained at the N intakes of $246mg\;N/kg\;W^{0.75}/d$. Thus, on a UMS-based diet supplementation of MOC up to 800 g (10% of total intake) of the dietary intake had little or no effect on intake, digestibility, rumen parameters, and microbial N yield but slightly increased the N balance. However marginal response to MOC supplementation is probably due to the high degradability of MOC protein in the rumen. Thus, any substantial positive response of MOC supplementation on a UMS-based diet can probably be achieved by reducing its protein degradability in the rumen.

Analysis of PIG Dynamics through Curved Section in Natural Gas Pipeline (천연가스 배관 곡관부에서의 피그 동적 거동 해석)

  • Kim D. K.;Nguyen T. T.;Yoo H. R.;Rho Y. W.;Kho Y.T.;Kim S. B.
    • Journal of the Korean Institute of Gas
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    • v.6 no.1 s.17
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    • pp.1-9
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    • 2002
  • This paper presents simple models for flow and the PIG dynamics when it passes through a $90^{\circ}$ curved section of pipeline. The simulation has been done with two different operational boundary conditions. The solution fur non-linear hyperbolic partial equations for flow is given by using MOC. The Runge-Kuta method is used to solve the initial condition equation fur flow and the PIG dynamics equation. The simulation results show that the proposed model and solution can be used fur estimating the PIG dynamics when the pig runs in the pipeline including curved section. In this paper, dynamic modeling and its analysis for the PIG flow through $90^{\circ}$ curved pipe with compressible and unsteady flow are studied. The PIG dynamics model is derived by using Lagrange equation under assumption that it passes through 3 different sections in the curved pipeline such that it moves into, inside and out of the curved section. The downstream and up stream flow dynamics including the curved sections are solved using MOC. The effectiveness of the derived mathematical models is estimated by simulation results fur a low pressure natural gas pipeline including downward and upward curved sections. The simulation results show that the proposed model and solution can be used for estimating the PIG dynamics when we pig the pipeline including curved section.

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APOLLO2 YEAR 2010

  • Sanchez, Richard;Zmijarevi, Igor;Coste-Delclaux, M.;Masiello, Emiliano;Santandrea, Simone;Martinolli, Emanuele;Villate, Laurence;Schwartz, Nadine;Guler, Nathalie
    • Nuclear Engineering and Technology
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    • v.42 no.5
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    • pp.474-499
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    • 2010
  • This paper presents the mostortant developments implemented in the APOLLO2 spectral code since its last general presentation at the 1999 M&C conference in Madrid. APOLLO2 has been provided with new capabilities in the domain of cross section self-shielding, including mixture effects and transfer matrix self-shielding, new or improved flux solvers (CPM for RZ geometry, heterogeneous cells for short MOC and the linear-surface scheme for long MOC), improved acceleration techniques ($DP_1$), that are also applied to thermal and external iterations, and a number of sophisticated modules and tools to help user calculations. The method of characteristics, which took over the collision probability method as the main flux solver of the code, allows for whole core two-dimensional heterogeneous calculations. A flux reconstruction technique leads to fast albeit accurate solutions used for industrial applications. The APOLLO2 code has been integrated (APOLLO2-A) within the $ARCADIA^{(R)}$ reactor code system of AREVA as cross section generator for PWR and BWR fuel assemblies. APOLLO2 is also extensively used by Electricite de France within its reactor calculation chain. A number of numerical examples are presented to illustrate APOLLO2 accuracy by comparison to Monte Carlo reference calculations. Results of the validation program are compared to the measured values on power plants and critical experiments.

Improvement Strategy of R&D Support to the IT Enterprises (IT 기업의 R&D 지원 개선 전략)

  • Choi, Se-Ha;Song, Hag-Hyun;Kim, Yoon-Ho
    • Journal of The Institute of Information and Telecommunication Facilities Engineering
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    • v.2 no.2
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    • pp.14-21
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    • 2003
  • In 1997, Korean IT enterprises were 9,000 in numbers and Gross Domestic Product (GDP) took 8.6%, and IT industry employed 400,000 persons. In 2002, it has increased : 21,000 companies, 14.9% of GDP, and 500,000 employees. National policy have diverse programs to the IT industry sector. Ministry of (MOC or MIC) have invested the R&D Program since late 1980. National subsidizes have been provided directly or indirectly to the programs. Direct subsidizes policy conflicts to the principle of the free competition market but it's very powerful to the IT organization (such as institutes, colleges, and companies) for the IT industry. This paper analyzes the national R&D subsidy systems and suggests the advanced systems.

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Government Strategy for Improvement of R&D Support to the W Enterprises (IT기업에 대한 정부의 R&D 지원 개선 방안)

  • 송학현;최세하;강희조;김윤호
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2003.05a
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    • pp.62-66
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    • 2003
  • In 1998, Korea IT company operation 10,000 and Gross Domestic Product(GDP) was 9.3%, IT industry employee 380,000 persons. In 2001, growing the company 20,000, GDP 12.9% and 500,000 employee. National policy have diverse programs to the IT sector. Ministry of communication(MOC or MIC) have invested by the R & D Program from later 1980'. National subsidizes direct or indirect to the programs, it's not market principal but subsidized to organization(Institute, college, company etc), this paper analysis the national subsidize system and suggestion the advanced system.

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A Study on Development of Certification Basis for VTOL UAS Based on Analysis of Certification Criteria for Fixed/Rotary Wing UAS and SC-VTOL (고정익/회전익 인증기준 및 수직이착륙 특수기술기준 분석 기반의 수직이착륙 무인항공기 인증기준 개발 방안)

  • Yoo, Minyoung;Kim, Suho;Oh, Yeonkyeong;Jin, Kyunghoon;Lee, Hwan;Kim, Woogyeom;Gong, Byeongho
    • Journal of Aerospace System Engineering
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    • v.15 no.5
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    • pp.16-23
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    • 2021
  • Domestic and foreign manufacturers are developing VTOL UASs in various shapes in line with demand for future technologies. UASs have been developed in a shape classified as fixed/rotary wing, and verified by appropriate certification standards. However, airworthiness certification of recent VTOL UASs is strict with the absence of VTOL-specific certification standards. In this paper, criteria applicable to VTOL UAS were presented through analysis of STANAG-4671 and STANAG-4702, which are certification standards for fixed/rotary wing UAS of the North Atlantic Treaty Organization (NATO) and the Special Condition for VTOL Aircraft (SC-VTOL) of European Aviation Safety Agency (EASA). For this, the categorization criteria of general/fixed-wing/VTOL characteristics were established for each standard item and utilized for analysis.

Energetics of the Heart Model with the Ventricu1ar Assist Device

  • Chung, Chanil-Chung;Lee, Sang-Woo;Han, Dong-Chul;Min, Byoung-Goo
    • Journal of Biomedical Engineering Research
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    • v.17 no.1
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    • pp.43-48
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    • 1996
  • We investigated the energistics of the physiological heart model by comparing predictive indexes of the myocardial oxygen consumption (MOC), such as tension-time index (R), tension-time or force-time inteual (FTI), rate-pressure product (RPP), pressure-work index, and systolic pressure-volume area (PVA) when using the electro-hydraulic left ventricular device (LVAD). We developed the model of LVAD incorporated the closed-loop cardiovascular system with a baroreceptor which can control heart rate and time-varying elastance of left and right ventricles. On considering the benefit of the LVAD, the effects of various operation modes, especially timing of assistance, were evaluated using this coupled computer model. Overall results of the computer simulation shows that our LVAD can unload the ischemic (less contractile) heart by decreasing the MU and increasing coronary flow. Because the pump ejection at the end diastolic phase of the natural heart may increase the afterload of the left ventricle, the control scheme of our LVAD must prohibit ejecting at this time. Since the increment of coronary flow is proportional to the peak aortic pressure after ventricle contraction, the LVAD must eject immediately following the closure of the aortic valve to increase oxygen availability.

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A comparative study of field measurements of the pressure wave with analytical aerodynamic model for the high speed train in tunnels (고속철도 터널내 압력파 측정과 공기압 해석모델에 대한 기초연구)

  • Kim, Hyo-Gyu;Choi, Pan-Gyu;Hong, Yoo-Jung;Yoo, Ji-Oh
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.17 no.3
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    • pp.319-332
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    • 2015
  • The pressure wave formed by the piston effects of the train proceeds within the tunnel when a train enters the tunnel with a high speed. Depending on the condition of tunnel exit, the compression waves reflect at a open end, change to the expansion waves, transfer to tunnel entrance back. Due to interference in the pressure waves and running train, passengers experience severe pressure fluctuations. And these pressure waves result in energy loss, noise, vibration, as well as in the passengers' ears. In this study, we performed comparison between numerical analysis and field experiments about the characteristics of the pressure waves transport in tunnel that appears when the train enter a tunnel and the variation of pressure penetrating into the train staterooms according to blockage ratio of train. In addition, a comparative study was carried out with the ThermoTun program to examine the applicability of the compressible 1-D model(based on the Method of Characteristics). Furthermore examination for the adequacy of the governing equations analysis based on compressible 1-D numerical model by Baron was examined.