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Herbicidal Response and Control of Scirpus juncoides Roxb. Resistant to Sulfonylurea Herbicides (Sulfonyurea계 제초제 저항성 올챙이고랭이(Scirpus juncoides Roxb.)의 제초제 반응과 방제)

  • Park, Tae-Seon;Kang, Dong-Kyun;Kim, Kil-Ung
    • The Korean Journal of Pesticide Science
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    • v.9 no.3
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    • pp.250-261
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    • 2005
  • This study was conducted to investigate the herbicidal response and effective control strategy of sulfonylurea(SU)-resistant Scirpus juncoides Roxb. occurred in the paddy fields of Korea. A biotype of Scirpus juncoides Roxb. resistant to SU was identified in the paddy fields treated with SU herbicide-based mixtures for seven consecutive years. The apparent SU resistance observed in Scirpus juncoides Roxb. was completely confirmed in greenhouse tests. The susceptible biotype was almost controlled at the recommended dose of all the tested, but the resistant biotype was survived 20 to 30% even at 10 times higher dose of each the recommended dose of SU herbicides. The $GR_{50}$ values of 4 SU herbicides for the resistant biotype were 53 to 88 times higher than those for the susceptible biotype. The acetolactate synthase(ALS) isolated from the resistant biotype against bensulfuron-methyl and pyrazosulfuron-ethyl was less sensitive than that of the susceptible biotype. The $I_{50}$ values of the resistant biotype against bensulfuron-methyl and pyrazosulfuron-ethyl were 498 and 126 times higher than those for the susceptible biotype. A rapid diagnosis for identifying resistance of Scirpus juncoides Roxb. was possible within at least 3 days after SU herbicides. Three herbicides having different mode of action from SU herbicide, carfentrazone-ethyl, pyrazolate and simetryne exhibited excellent controlling effects on the resistant biotype of Scirpus juncoides Roxb. till 3.5 leaf stage. Among the SU-based herbicides, pyriminobac-methyl+pyrazosulfuroil-ethyl+carfentrazone-ethyl GR and azimsulfuron+carfentrazone-ethyl+pyriminobac-methyl GR were very effective to control resistant biotype of Scirpus juncoides Roxb. without rice injury. The resistant biotype which were not controlled with SU herbicise-based herbicides survived from the fields were effectively controlled by bentazone SL.

Seasoning of Commercial Wood Using Solar Energy (태양에너지를 이용한 유용목재의 건조)

  • Jung, Hee-Suk;Lee, Hyoung-Woo;Lee, Nam-Ho;Lee, Sang-Bong
    • Journal of the Korean Wood Science and Technology
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    • v.16 no.4
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    • pp.10-39
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    • 1988
  • This study investigated the temperatures and relative humidities in the semi-greenhouse type solar dryer with a black rock-bed heat storage and without heat storage and outdoor temperature and relative humidity at 9 a.m. and 2 p.m.. A comparison was made of the drying rates, final moisture contents, moisture content distributions, casehardening stresses, drying defects, volumetric shrinkage of dried lumber for solar- and air-drying from the green condition of mixtures of Douglas-fir, lauan, taun, oak and sycamore 25mm- and 50 mm-thick lumber during the same period for four seasons, and heat efficiencies for solar dryer with and without the heat storage for saving of heat energy and the cost of lumber drying using the solar energy. The results from this study were summarized as follows: I. The mean weekly temperatures in the solar dryers were 3 to $6^{\circ}C$ at 9 a.m. and 9 to $13^{\circ}C$ at 2 p.m. higher than mean outdoor temperature during all the drying period. 2. The mean weekly relative humidities in the solar dryers were about 1 to 19% at 9 a.m. higher than the outdoor relative humidity. and the difference between indoor and outdoor relative humidity in the morning was greater than in the afternoon. 3. The temperatures and relative humidities in the solar dryer with and without the heat storage were nearly same. 4. The overall solar insolation during the spring months was highest and then was greater in the order of summer, atumm, and winter month. S. The initial rate of solar drying was more rapid than that of air drying. As moisture content decreased, solar drying rate became more rapid than that of air drying. The rates of solar drying with and without heat storage were nearly same. The drying rate of Douglas-fir was fastest and then faster in the order of sycamore, lauan, taun and oak. and the faster drying rate of species, the smaller differences of drying rates between thicknesses of lumber. The drying rates were fastest in the summer and slowest in the winter. The rates of solar drying during the spring were more slowly in the early stage and faster in the later stage than those during the autumn. 6. The final moisture contents were above 15% for 25mm-thick air dried and about 10% for solar dried lumber, but the mean final MCs for 50mm-thick lumber were much higher than those of thin lumber. The differences of final MC between upper and lower course of pile for solar drying were greater than those of pile for air drying. The differences of moisture content between the shell and the core of air dried lumbers were greater than those of solar dried lumber, smallest in the drying during summer and greatest in the drying during winter among seasons. 7. Casehardening stresses of 25mm- and 50mm-thick dried lumber were slight, casehardening stress of solar dried lumber was severer than that of air dried lumber and was similar between solar dried lumber with and without heat storage, Casehardening stresses of lumber dried during spring were slightest and then slighter in the order of summer, autumn, and winter. Casehardening stresses of Douglas -fir, sycamore and lauan were slight, comparing with those of taun and oak. 8. Maximum initial checks of 25mm-thick lumber occurred above and below fiber saturation point and those of 50mm-thick lumber occurred in the higher moisture content than thin lumber. As the moisture content decreased, most of checks were closed and didn't show distinct difference of the degree of checks among drying methods. The degree of checks were very slight in case of Douglas-fir and lauan, and severe in case of taun and oak. The degree of checks for 50mm-thick lumber were severer than those for 25mm-thick lumber. 9. The degree of warpage showed severe in case of oak and sycamore lumber, but no warping was found in case of Douglas-fir, lauan and taun. 10. The volumetric shrinkages of taun and oak were large and medium in case of Douglas-fir, lauan and sycamore. 11. Heat efficiencies of solar dryer with heat storage were 6.9% during spring, 7.7% during summer, 12.1% during autumn and 4.1% during winter season. Heat efficiency of solar dryer with heat storage was slightly greater than that of without heat storage. As moisture content of lumber decreased, heat efficiency decreased.

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Changes of Protein Profiles in Cheonggukjang during the Fermentation Period (전통 청국장의 발효 기간 동안 변화하는 수용성 단백질 개요)

  • Santos, Ilyn;Sohn, Il-Young;Choi, Hyun-Soo;Park, Sun-Min;Ryu, Sung-Hee;Kwon, Dae-Young;Park, Cheon-Seok;Kim, Jeong-Hwan;Kim, Jong-Sang;Lim, Jin-Kyu
    • Korean Journal of Food Science and Technology
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    • v.39 no.4
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    • pp.438-446
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    • 2007
  • The fermented soybean product, cheonggukjang, is favored by many people, partly due to its bio-functional ingredients. Since the fermentation process of cheonggukjang is mediated by enzymes, including proteases, produced by microbes, analysis of the proteome profile changes in cheonggukjang during fermentation would provide us with valuable information for fermentation optimization, as well as a better understanding of the formation mechanisms of the bio-functional substances. The soluble proteins from cheonggukjang were prepared by a phenol/chloroform extraction method, in order to remove interfering molecules for high resolution 2-D gel analysis. Proteomic analysis of the cheonggukjang different fermentation periods suggested that most of the soluble soy proteins were degraded into smaller forms within 20hr, and many microbial proteins, such as mucilage proteins, dominated the soluble protein fraction. The proteomic profile of cheonggukjang was very different from natto, in terms of the 2-D gel protein profile. Among the separated protein spots on the 2-D gels, 50 proteins from each gel were analyzed by MALDI-TOF MS and PMF for protein identification. Due to database limitations with regard to soy proteins and microbial proteins, identification of the changed proteins during fermentation was restricted to 9 proteins for cheonggukjang and 15 for natto. From de novo sequencing of the proteins by a tandem MS/MS, as well as by database searches using BLASTP, a limited number of proteins were identified with low reliability. However, the 2-D gel analysis of proteins, including protein preparation methods, remains a valuable tool to analyze complex mixtures of proteins entirely. Also, for intensive mass spectrometric analysis, it is also advisable to focus on a few of the interestingly changed proteins in cheonggukjang.

Development of Multiplex PCR Assay for Identification of Eight Species from Meats in Korea (국내에서 유통되는 8종의 식육감별을 위한 multiplex PCR법 개발)

  • Heo, Eun-Jeong;Ko, Eun-Kyung;Yoon, Hyang-Jin;Kim, Yeon-Hwa;Kim, Young-Jo;Park, Hyun-Jung;Wee, Sung-Hwan;Moon, Jin-San
    • Journal of Food Hygiene and Safety
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    • v.31 no.1
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    • pp.28-35
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    • 2016
  • Species identification of animal tissues in meat products is an important issue to protect the consumer from illegal and/or undesirable adulteration; for economic, religious and health reasons. In this reason, accurate analytical methods are needed for the labeling of meat products with requiring simple and fast procedure. Recently, applications of PCR in food analysis have been increased because of their simplicity, specificity and sensitivity. Therefore, in this study, a multiplex PCR assay was developed for the simultaneous identification of eight species of cow, pig, chicken, duck, goat, sheep, horse and turkey from raw meats. The primers were designed in different regions of mitochondrial 16S RNA after alignment of the available sequences in the GenBank database. Two multiplex primer sets were designed as Set 1 (cow, pig, chicken, duck) and Set 2 (goat, sheep, horse, turkey), respectively. Total 274 samples from cow (n = 55), pig (n = 30), chicken (n=30), and duck (n = 30), goat (n = 40), sheep (n = 33), horse (n = 41), and turkey (n = 15) were tested. The primers generated specific fragments of 94, 192, 279, 477 bp (pig, chicken, cow, duck), 670, 271, 152, 469 bp (goat, sheep, horse, turkey) lengths for eight species, respectively. The animal species specificity was 100% in all eight samples in the multiplex PCR assay. The detection limit of the multiplex PCR assay showed from 100 fg to 1 pg of template DNA from extracted from raw meats. When applying multiplex PCR assays to sample from pork/beef and pork/chicken, beef/chicken tested raw mixed meats and heat-treated ($83^{\circ}C$ for 30min, $100^{\circ}C$ for 20min, and $121^{\circ}C$ for 10min) mixtures, detection limit was 0.1% level beef, pork and pork in beef and chicken in pork and 1.0% level pork in chicken. This study suggest that the developed multiplex PCR assay can be used for rapid and simultaneous species identification of cow, pig, chicken, duck, goat, sheep, horse and turkey from meats.

Effect of Lentil and Opuntia ficus-indica Mixtures Addition on Quality Characteristics of Sausages (렌틸과 백년초의 혼합첨가가 소시지의 품질특성에 미치는 영향)

  • Lee, Namrye;Kim, Kyoung Hee;Yook, Hong Sun
    • Korean journal of food and cookery science
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    • v.31 no.4
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    • pp.431-440
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    • 2015
  • This study was performed to evaluate the quality characteristics of sausages after addition of lentil and Opuntia ficus-indica ethanol extract. Seven sausages were prepared as follows : F0 (control), F1 (5% lentils), F2 (5% lentils + 1% Opuntia ficus-indica), F3 (5% lentils + 3% Opuntia ficus-indica), F4(10% lentils), F5 (10% lentils + 1% Opuntia ficus-indica), and F6 (10% lentils + 3% Opuntia ficus-indica). Addition of lentils increase dietary fiber and starch in sausage while lowering fat content. Starch is used in manufacturing sausage to stabilize and increase viscosity. Opuntia ficus-indica contains dietary fibers and therefore addition of it to sausage increases dietary fiber, much like lentil addition. Lightness decreased and yellowness increased in all treatments. Redness was lowered by lentil addition but enhanced by addition of Opuntia ficus-indica. Redness in F3 and F5 were similar with control. But, F5 was more similar with control in all colors. Addition of lentil and Opuntia ficus-indica improved texture in hardness, springiness, gumminess, and chewiness. In sensory evaluation, color was lowered but taste was heightened by adding lentil and Opuntia ficus-indica extract. From results of this study, we could conclude that addition of mixture of lentil and Opuntia ficus-indica made sausage low in fat, with high in dietary fibers and starch. In addition, texture was increased and taste was better. F5 had the most similar color to control. We found out the optimal amounts of the two ingredients, lentil and Opuntia ficus- indica extract, were 10% and 1%, respectively.

Binder Stiffness Effect on Permanent Deformation and Tensile Strength of Asphalt Concretes (바인더 강성이 아스팔트 콘크리트의 인장강도와 소성변형 특성에 미치는 영향 분석)

  • Kim, Hyun-Hwan;Yoo, Min-Yong;Kim, Jin-Chul;Kim, Kwang-Woo
    • International Journal of Highway Engineering
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    • v.12 no.2
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    • pp.17-23
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    • 2010
  • Since the relatively stiff binder shows a higher tensile strength as well as higher rutting resistance, it is believed that the binder stiffness is an important factor for rutting and tensile strength of asphalt mixtures. The typical tensile property is measured by indirect tensile strength (ITS) test at $25^{\circ}C$ and the rutting resistance is most widely measured by wheel tracking (WT) test at $60^{\circ}C$. The deformation strength ($S_D$) is newly developed property to estimate rut resistance of asphalt concretes at $60^{\circ}C$. The ITS and $S_D$ are very simple to measure by static test techniques, but the WT is measured by repeated loading procedure which requires relatively longer time and more efforts. Since these three properties are highly dependent upon the binder stiffness, it may be possible to estimate one property from another. Therefore, this study investigate the possibility of estimating the rutting characteristics (measured by WT test) by ITS or $S_D$ test, and the ITS by $S_D$. Because of binder stiffness effect, in the WT estimation by ITS, a tendency was observed for the higher ITS mixture to have the lower rut depth, giving $R^2{\fallingdotseq}$0.6, on the average. The ITS estimation by $S_D$ showed $R^2{\fallingdotseq}$0.64, and the WT estimation by SD showed $R^2{\fallingdotseq}$0.84, which is highest correlation among the three. Therefore, it was concluded that there is relatively good possibility of estimating WT result by $S_D$, and even though $R^2$ is somewhat low, there is some correlation between WT and ITS.

Effects of Medium and Planting Density on Growth and Yield of Seed Potatoes Grown in a Wick Hydroponic System (배지 및 재식밀도가 심지양액재배 씨감자의 생육 및 수량에 미치는 영향)

  • Kim, Chan-Woo;Song, Chang-Khil;Park, Jung-Sik;Mun, Hyun-Ki;Kang, Young-Kil;Kang, Bong-Kyoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.251-255
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    • 2008
  • This study was carried out in 2002 to determine the usefulness of Jeju scoria for a component of a growth medium and optimum planting density of 'Dejima' seed potatoes (Solanum tuberosum L.) grown in a wick hydroponic system. The minitubers ($7.0{\pm}0.2\;g$) produced in an aeroponics system were planted at five planting densities (3 to 15 tubers/box; 19 to $95\;tubers/m^2$) in polystyrene boxes (51 cm long $\times$ 31 cm wide $\times$ 20 cm high) containing two media (perlite + peatmoss and Jeju scoria + peatmoss 1:2, v/v mixtures). There were no significant interactions between medium and planting density for the growth and tuber yield traits. Shoot growth and the number of tubers per plant were not significantly affected by the media. However, tuber yield was higher in the perlite + peatmoss mixture than in the Jeju scoria + peatmoss mixture. The percentage of underdeveloped plants ranged from 8.3 to 14.7% at four lower planting densities (3 to 12 tubers/ box), and was 25.8% at the highest planting density (15 tubers/box). As planting density was increased from 3 to 15 tubers per box, seed potato (${\geq}5\;g$ tuber) number increased from 101 to 269 and yield from 6.3 to $11.6\;kg/m^2$. These results indicate that the perlite + peatmoss mixture might be more suitable for seed potato production in the wick hydroponic system, and considering the percentage of underdeveloped plants and tuber yield, optimum planting density would be 56 to $76\;tubers/m^2$ in the system depending on availability of seed potatoes.

Stable isotope, Fluid Inclusion and Mineralogical Studies of the Samkwang Gold-Silver Deposits, Republic of Korea (삼광 금-은광상의 산출광물, 유체포유물 및 안정동위원소 연구)

  • 유봉철;이현구;최선규
    • Economic and Environmental Geology
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    • v.35 no.4
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    • pp.299-316
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    • 2002
  • The Samkwang gold-silver deposits consist of gold-silver-bearing hydrothermal massive quartz veins which filled the fractures along fault shear (NE, NW) zones within Precambrian banded or granitic gneiss of Gyeonggi massif. Ore mineralization of this deposits occurred within a single stage of quartz vein which was formed by multiple episodes of fracturing and healing. Based on vein mineralogy and paragenesis, massive quartz veins are divided into two main paragenetic stages which are separated by a major faulting event. Main ore mineralization occurred at stage I. Wall-rock alteration from this deposits occur as mainly sericitization, chloritization, silicification and minor amounts of pyritization, carbonitization, propylitization and argillitization. Ore minerals are composed mainly of arsenopyrite (29.21-32.24 As atomic %), pyrite, sphalerite (6.45-13.82 FeS mole %), chalcopyrite, galena with minor amounts of pyrrhotite, marcasite, electmm (39.98-66.82 Au atomic %) and argentite. Systematic studies of fluid inclusions in early quartz veins and microcracks indicate two contrasting physical-chemical conditions: 1). temperature (215-345$^{\circ}$C) and pressure (1296-2022 bar) event with $H_{2}O-CO_{2}-CH_{4}-NaCl$fluids (0.8-6.3 wt. %) related to the early sulfide deposition, 2). temperature (203-441$^{\circ}$C) and pressure (320 bar) event with $H2_{O}$-NaCI $\pm$ $CO_{2}$ fluids (5.7-8.8 wt. %) related to the late sulfide and electrum assemblage. The H20-NaCI $\pm$ $CO_{2}$ fluids represent fluids evolved through fluid unmixing of an $H_{2}O-CO_{2}-CH_{4}-NaCl$fluids due to decreases in fluid pressure and influenced of deepcirculated meteoric waters possibly related to uplift and unloading of the mineralizing suites. Calculated sulfur isotope compositions (${\delta}^{34}S_{fluid}$) of hydrothermal fluids (1.8-4.9$\textperthousand$) indicate that ore sulfur was derived from an igneous source. Measured and calculated oxygen and hydrogen isotope compositions (${\delta}^{18}O_{I120}$, ${\delta}D$) of ore fluids (-5.9~10.9$\textperthousand$, -102~-87$\textperthousand$) indicate that mesothermal auriferous fluids at Samkwang gold-silver deposits were likely mixtures of $H_{2}O$-rich, isotopically less evolved meteoric water and magmatic fluids.

Geochemical Characteristics of Black Slate and Coaly Slate from the Uranium Deposit in Deokpyeong Area (덕평리 지역 우라늄광상의 흑색점판암과 탄질점판암의 지구화학적 특성)

  • Shin, Dong-Bok;Kim, Su-Jeong
    • Economic and Environmental Geology
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    • v.44 no.5
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    • pp.373-386
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    • 2011
  • Geochemical study was performed on black slates and interbedded U-bearing coaly slates in Deokpyeongri area, the representative uranium mineralized district of the Ogcheon Metamorphic Belt, to discuss the genetic environments of the uranium deposit. REE concentration (254 ppm) of the black slates is higher than that (169 ppm) of the coaly slates and NASC-normalized REE patterns of the coaly slates show remarkable positive Eu anomaly. l11e redox-sensitive trace elements such as V, Cr, Co, Ni, Mo and U in the coaly slates are highly enriched compared to the black slates, especially for V of 24 times, Mo of 62 times, and U of 60 times. In additions, Pd and Pt are also enriched in the coaly slates. Positive Eu anomaly and the noticeable enrichment of the elements listed above compared to those of NASC indicate that those elements were not derived from common seawater but deposited under high temperature and reducing environment of submarine hydrothermal activities. Wide compositional ranges of major elements ($SiO_2/Al_2O_3$: 3.98~11.88, $Al_2O_3/Na_2O$: 25.6~139.06, $K_2O/Na_2O$: 6.80~46.85) also suggest that the source rocks of the sediments are mixtures of sedimentary rocks and igneous rocks. Higher sulfur contents in the coaly slates, 2.6 wt.%, than those in the black slates, 0.6 wt.% also indicates that the former was influenced by hydrothermal activities containing much sulfur. These geochemical characteristics are similar to the genetic environments of South China type PGE deposits (Mo-Ni-Zn-PGE) which is geotectonically correlated with the Ogcheon Metamorphic Belt and is known as sedimentary-exhalative deposits. In conclusions, the uranium and other metallic elements mineralization seems to have occurred in the sedimentary basin that was affected by submarine hydrothermal activities and rich in organic materials under oxygen-poor environments as well.

Optimization Test of Plant-Mineral Composites to Control Nuisance Phytoplankton Aggregates in Eutrophic Reservoir (부영양 저수지의 조류제거를 위한 기능성 천연물질혼합제의 최적화 연구)

  • Lee, Ju-Hwan;Kim, Baik-Ho;Moon, Byeong-Cheon;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.44 no.1
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    • pp.31-41
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    • 2011
  • To optimize the natural chemical agents against nuisance phytoplankton, we examined algal removal activity (ABA) of Plant-Mineral Composite (PMC), which already developed by our teams (Kim et al., 2010), on various conditions. The PMC are consisted of extracted-mixtures with indigenous plants (Camellia sinensis, Quercusacutissima and Castanea crenata) and minerals (Loess, Quartz porphyry, and natural zeolite), and characterized by coagulation and floating of low-density suspended solids. A simple extraction process was adopted, such as drying and grinding of raw material, water-extraction by high temperature-sonication and filtering. All tests were performed in 3 L plastic chambers varying conditions; six different concentrations ($0{\sim}1.0\;mL\;L^{-1}$), six light intensities ($8{\sim}1,400\;{\mu}mol\;m^{-2}s^{-1}$), three temperatures ($10{\sim}30^{\circ}C$), four pHs (7~10), five water depths (10~50 cm), and three different waters dominated by cyanobacteria, diatom, and green algae, respectively. Results indicate that the highest ABA of PMC was seen at $0.05\;mL\;L^{-1}$ in treatment concentrations, where showed a reduction of more than 80% of control phytoplankton biomass, while $1,400\;{\mu}mol\;m^{-2}s^{-1}$ in light intensity (>90%), $20{\sim}30^{\circ}C$ temperature (>60%), 7~9 in pH (>90%), below 50 cm in water depth (>90%), and cyanobacterial dominating waters (>80%), respectively. Over the test, ABA of PMC were more obvious on the algal biomass (chlorophyll-${\alpha}$) than suspended solids, suggesting a selectivity of PMC to particle size or natures. These results suggest that PMC agents can play an important role as natural agents to remove the nuisant algal aggregates or seston of eutrophic lake, where occur cyanobacterial bloom in a shallow shore of lake during warm season.