• 대한화학회지
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• 제53권6호
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• pp.742-748
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• 2009

정육 사업에서 고기의 원산지와 종을 표기 하는 것은 육질의 판단에 영향을 미친다. 유전자 마커는 종을 판별하기 위한 증거로 사용되므로, 우리는 한우와 젖소 그리고 혼입육을 판단 할 수 있는 유전자 마커의 개발 계획을 수립하였다. 소의 모든 종은 모색 유전자에 의하여 그 종이 결정 되며 모색 유전자의 조절에 의하여 유멜라닌 또는 페오멜라닌이 합성되고, 이로 인하여 모색에 차이가 생기는 점을 이용하여, 종을 판별하는 유전자 마커로 사용된다. 암소와 수소를 판별하기 위하여 Y 염색체 상에 존재하는 성 결정 부위 유전자에 대응하는 프라이머를 제작하였다. 본 연구에서는 모색 유전자와 성 결정 유전자를 다중 중합효소연쇄반응(multiplex-PCR)을 이용하여 증폭하였고, 증폭 산물을 제한 효소 MspA1I로 소화 시켰다. 이 반응물을 변성 고성능 액체 크로마토그래피(denaturing high performance liquid chromatography, DHPLC)를 이용하여 분석하였다. 분석 결과 6가지의 크로마토그램을 확인 할 수 있었고, DHPLC 분석 방법은 한우, 젖소 그리고 혼입육을 쉽게 구별해 낼 수 있었다.

• 한국안광학회지
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• 제18권1호
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• pp.53-59
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• 2013

목적: 40세 이상 한국 성인남녀에서 수정체의 혼탁 종류에 따른 백내장의 유형별 유병률을 산출하고 교정시력을 알아보았다. 방법: 제4기 국민건강영양조사(NHANES) 중 2009년 자료를 이용하여 인구 기반 단면 연구로 디자인하였다. 40세-95세까지 총 4,977명에 대해 연령과 성별에 따라 층화하여 백내장의 유병률과 수정체 혼탁의 종류에 따른 유병률을 조사하였다. 전체 대상자의 평균 연령은 $58.28{\pm}12.07$세 였고, 남자가 2,142명(43.0%), 여자가 2,835명(57.0%)이었다. 교정시력은 0.63 미만인 경우를 산출하였다. 결과: 전체대상자 4,977명 중에서 현재 백내장이 있는 경우는 40.6%(남자 17.5%, 여자 23.1%)로 나타났다. 백내장의 유형별 유병률은 핵형(56.3%), 피질형(23.4%), 혼합형(16.2%), 전낭하(3.0%), 후낭하(1.1%) 순으로 나타났다. 유형별 남녀의 차이는 없었고, 모든 연령대에서 핵형이 가장 많았다. 전낭하 백내장은 대부분의 경우에서 교정시력이 0.63 미만으로 낮았다. 결론: 핵형 백내장의 유병률이 가장 높았고, 수정체의 혼탁을 고려한 역학 연구와 예방 정책의 수립이 필요할 것으로 사료된다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권2호
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• pp.170-177
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• 2005

The pleomorphic adenoma is well recognized as the most common salivary neoplasm. We examined 49 patients who had received surgical excision of the pleomorphic adenoma from 1989 to 1998 with over 5 years follow-up period. We retrospectively evaluated the patients' age, sex, chief complaints, surgical methods, and recurrence or complication rates after analysis of one's clinical and surgical records. The results are as follows : 1. There were 15 cases in parotid gland, 23 cases in palate, 8 cases in submandibular gland, and 3 cases in cheek. The ratio of male to female was 1 : 1.13. The mean age was 44. The tumor of submandibular gland occurred in more younger age than that of other salivary gland. 2. In 15 patients of parotid pleomorphic adenoma, there was 1 case(6.7%, 1/15) of recurrence. That was transformed into the malignant pleomorphic adenoma after 4 years of first surgery. We performed superficial parotidectomy of 9 cases(56.2%, 9/16), total parotidectomy of 6 cases(37.5%, 6/16), and radical parotidectomy of 1 case(6.3%, 1/16). 3. We used the rotational Sternocleidomastoid muscular flap to cover the exposed facial nerve in 12 cases(75%) after parotidectomy(7 cases of superficial parotidectomy and 5 cases of total parotidectomy). We could see 3 cases(18.7%) of facial nerve palsy and 1 case(6.3%) of Frey's syndrome after parotidectomy. We examined Frey's syndrome in only 1 case which was not used SCM muscular flap after parotidectomy. 4. In 23 patients of palatal pleomorphic adenoma, there were 2 cases(8.7%) of recurrence. In recurrence cases, We performed re-excision after 4 and 5 years of first surgery, respectively. We preserved partial thin overlying palatal mucosa during tumor excision in 5 cases(20%), which were proved as benign mixed tumor in preoperative biopsy. That mucosa-preserved cases had thick palatal mucosa, did not show mucosa ulceration and revealed well encapsulated lesions in preoperative CT. 5. In palatal tumors, we could see the 13 cases(52%) of bony invasion in preoperative CT views and the 4 cases(16%) of oro-nasal fistula after tumor excision. In two cases of recurrence, one(20%, 1/5) was in palatal mucosa-preserved group and the other(5.5%, 1/18) was in palatal mucosa-excised group. 6. We excised tumors with submandibular glands in the all cases of submandibular pleomorphic adenoma. There was no specific complication or recurrence in these cases. 7. After excision of the cheek pleomorphic adenomas, we could not see any complication or recurrence.

• Asian-Australasian Journal of Animal Sciences
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• 제28권12호
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• pp.1767-1773
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• 2015

Effects of water-misting sprays with forced ventilation after transport during summer on meat quality, stress parameters, glycolytic potential and microstructures of muscle in broilers were investigated. A total of 105 mixed-sex Arbor Acres broilers were divided into three treatment groups: i) 45-min transport without rest (T group), ii) 45-min transport with 1-h rest (TR group), iii) 45-min transport with 15-min water-misting sprays with forced ventilation and 45-min rest (TWFR group). The results showed the TWFR group significantly increased (p<0.05) initial muscle pH ($pH_i$) and ultimate pH ($pH_u$) and significantly reduced $L^*$ (p<0.05), drip loss, cook loss, creatine kinase, lactate dehydrogenase activity, plasma glucose content, lactate and glycolytic potential when compared with other groups. Microstructure of the muscle from TWFR group broilers under light microscopy showed smaller intercellular spaces among muscle fibers and bundles compared with T group. In conclusion this study indicated water-misting sprays with forced ventilation after transport could relieve the stress caused by transport under high temperature, which was favorable for the broilers' welfare. Furthermore, water-misting sprays with forced ventilation after transport slowed down the postmortem glycolysis rate and inhibited the occurrence of PSE-like meat in broilers. Although rest after transport could also improve the meat quality, the effect was not as significant as water-misting sprays with forced ventilation after transport.

• 한국산림과학회지
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• 제105권1호
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• pp.42-47
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• 2016

천연활엽수림의 천연갱신 가능성을 파악하고자, 당단풍나무의 개화 결실, 종자낙하, 종자활력을 강원도 평창군 중왕산 지역에서 2009년부터 2015년까지 조사하였다. 당단풍나무 꽃은 산방화서에 양성화와 웅성화가 달렸으며, 화서 내에서 성형태간 개화시기가 달랐다. 양성화가 먼저 개화하는 개체에서는 암술이 수분되어 자라기 시작한 꽃에서는 수술은 더 이상 성숙하지 않고 소실되었으며, 웅성화가 먼저 개화하는 수술이 발달한 꽃에는 암술은 흔적으로만 존재하였다. 다른 개체에서의 화분이 꿀벌, 애꽃벌류 및 꽃등에류에 의하여 수분이 이루어지는 이형자웅이숙(heterodichogamy)의 성 형태로 자가수분을 회피하는 기작을 가지고 있었다. 종자 낙하량은 310~234,840 ea/ha의 범위로 평균은 70,780 ea/ha이었다. 종자생산에 있어 연년변동이 심하며, 최대치가 최소치의 760배 가량이었다. 당단풍나무의 연년종자생산은 정규분포하는 것이라 사료된다. 성숙한 종자 중에서 피해 또는 부후종자가 59.3%로 가장 많았고, 건전종자가 23.9%, 미발육 종자 9.2%, 쭉정이종자 7.6% 등으로 나타났다. 건전종자 생산량을 좌우하는 요인은 개화기인 5월 중,하순에 화분매개충의 활동과 어린 자방이 자라는 시기에 흡즙성 해충의 밀도와 활동성이 가장 중요한 요인이라 사료된다. 당단풍나무의 천연갱신은 종자풍년 이듬해에 발아와 생장에 유리한 조건을 갖춘 갭에서 이루어질 것으로 사료된다. 낙엽활엽수의 천연갱신을 이해하기 위해서 개화결실 특성과 비산 전후 종자 피식 문제에 대한 장기간의 연구가 필요할 것이라 사료된다.

• Journal of Acupuncture Research
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• 제23권3호
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• pp.21-35
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• 2006

Objectives : Herniation of Nucleus Pulposus(HNP) of Cervical is the most important reason that causes Cervical pain. The aim of this study is to investigate the effectiveness of Carthmi-Flos Herbal acua-acupuncture therapy for Cervical disc Herniation patients. Methods : To evaluate the effectiveness of Carthmi-Flos Herbal acua-Acupuncture Therapy, 20 patients were treated by Carthmi-Flos Herbal acua-acupuncture therapy. To estimate the efficacy of treatment, we used Quardruple Visual Analog Scale (QVAS). Results & Conclusion : 1. As a objectivity treatment record, they test treatment record good 35%, fair 35%, excellent 30%. 2. After Carthmi-Flos acua-acupuncture therapy, pain rate changed from 8.80 to 5.40 3. By the results which puts out the statistics in sex , a pain rate of male changed from 8.57$({\pm}1.51)$ to 2.71$({\pm}2.63)$, a pain rate of female 8.92$({\pm}0.95)$ to 3.77$({\pm}2.09)$. Intentional difference is none as a therapy. 4. By the results which puts out the statistics in age, after forties changed from 9.07$({\pm}0.88)$ to 3.80$({\pm}2.43)$ and before forties changed from 8.00$({\pm}1.58)$ to 2.20$({\pm}1.30)$. Intentional difference is none as a therapy. 5. By the results which puts out the statistics in Duration, pain rate of the acutest group changed from 8.36$({\pm}1.12)$ to 2.18$({\pm}1.08)$, pain rate of the subacute group changed from 9.80$({\pm}0.45)$, to 5.20$({\pm}2.68)$, pain rate of the chronic group changed from 8.67$({\pm}1.53)$ to 3.33$({\pm}1.53)$. Intentional difference is none as a therapy. 6. By the results which puts out the statistics in the reading of Lumbar MID, pain rate of the Bulging type changed from 9.00$({\pm}1.05)$ to 4.30$({\pm}2.67)$, pain rate of the Protruded type changed from 8.50$({\pm}1.00)$, to 3.50$({\pm}1.29)$, pain rate of the Extruded type changed from 6.00 to 1.00, pain rate of the Mixed type changed from 9.20$({\pm}0.84)$ to 2.00$({\pm}1.23)$, Intentional difference is none as a therapy.

• Journal of Preventive Medicine and Public Health
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• 제31권4호
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• pp.628-643
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• 1998

To investigate the exposure levels of organic solvents and subjective symptoms of dry-cleaning workers, 77 male and 52 female dry-cleaning workers who had been worked in a small city of Chungnam province, and a large city, Taejon were selected for the study group. Air concentrations of organic solvents in the working environment were analyzed, and subjective symptoms of dry-cleaning workers were surveyed, from July to August 1996. The results obtained were as follows : 1. The concentrations of organic solvents in the working environment were within permissible TLV-TWA limits. 2. For the 13 symptom clusters, the most frequently complained symptom clusters were fatigue as 71.3%, and followed by depression and urinary disturbances as 53.5% and 51.9%. Other symptom clusters complained were below 50%. 3. Positive response rates of subjective symptoms were significantly higher in worker groups such as lived in a large city, female, higher education level, more frequently alcohol drinking, higher concentration of organic solvent in working environment, work in alone. 4. Workers who had used solvent B showed 2.3 point higher scores of subjective symptoms than those of solvent A. Of the subjective symptoms scores, amnesia and nervousness were higher in solvent B user group than solvent B user group. 5. As a result of factor analysis, 3 factors such as depression, urinary disturbance and neurologic disturbance were selected. 6. As a result of the logistic regression analysis, sex, the number of fellow workers, working time, region, job tenure, smoking, alcohol drinking, ventilating system, concentration of organic solvent in working environment and place of residence were selected for the related variables. For the conclusion, even though the concentrations of organic solvents in the working environments of dry-cleaning workers were within permissible limit of TLV-TWA, many dry-cleaning workers complained symptoms, such as fatigue, depression, urinary disturbances and so on. And the factors affecting to the symptoms of dry-cleaning workers were the number of fellow workers, work hours, region, job tenure, smoking and alcohol drinking.

• 농업과학연구
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• 제3권2호
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• pp.192-197
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• 1976

IKUTON 발효(醱酵) 고구마 전분박(澱粉粕)의 농후사료대축수준(濃厚飼料代蓄水準) 調査하기 위하여 Large Yorkshire, Landrace, Duroc Jersey 삼품종(三品種) 교잡종(交雜種) 25두(頭)를 공시(供試)하여 농후사료(濃厚飼料)를 급여(給與)한 대조구(對照區)와 IKUTON 발효(醱酵) 고구마 전분박(澱粉粕) 10%, 20%, 30% 및 40%를 대치한 5개처리구(個處理區)를 완전임의배치(完全任意配置)하여 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 사료섭취량(飼料攝取量)은 40% 구(區)를 제외(除外)한 기타(其他)의 각처리구(各處理區)에서는 대조구(對照區)에 비(比)하여 유의적(有意的) (P<0.05)인 차이(差異)를 인정(認定)할 수 없었으며 40% 이상(以上)의 대체수준(代替水準)에서 사료섭취량(飼料攝取量)이 감소(減少)되고 있었다. 사료효율(飼料效率)은 20% 구(區); 3.43, 30% 구(區); 3.80, 10% 구(區) 3.97, 40% 구(區); 4.20, 0% 구(區0; 4.53의 순(順)으로 대조구(對照區)에 비(比)하여 높은 사료효율(飼料效率)을 나타내고 있었다. 증체량과 80kg 도달주수(到達週數)는 10% 구(區); 12주(週), 0%구(區); 13주(週), 20% 및 30%구(區)가 14주(週)였고 40%구(區)는 15주(週)까지도 80kg에 도달(到達)치 못하고 있었으며 대조구(對照區)에 비(比)하여 64%의 증체밖에 되지 않었다. 시험사료(試驗飼料)의 소화율(消化率)은 75.2~87.5%였으며 급수율(給水率)은 사료섭취(飼料攝取) 1kg당(當) $1.95-2.01{\ell}$로 되고 있어 사료급여회수(飼料給與回數)는 소화율(消化率)과 급수율(給水率)에 아무런 영향을 주지 않었다.

• 한국환경보건학회지
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• 제33권5호
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• pp.365-371
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• 2007

To evaluate the effect of air pollution on respiratory health in children, We conducted a longitudinal study in which children were asked to record their daily levels of Peak Expiratory Flow Rate(PEFR) using potable peak flow meter(mini-Wright) for 4 weeks. The relationship between daily PEFR and ambient air particle levels was analyzed using a mixed linear regression models including gender, age in year, weight, the presence of respiratory symptoms, and relative humidity as an extraneous variable. The daily mean concentrations of $PM_{10}$ and $PM_{2.5}$ over the study period were $64.9{\mu}g/m^3$ and $46.1{\mu}g/m^3$, respectively. The range of daily measured PEFR in this study was $182{\sim}481\;l/min$. Daily mean PEFR was regressed with the 24-hour average $PM_{10}(or\;PM_{2.5})$ levels, weather information such as air temperature and relative humidity, and individual characteristics including sex, weight, and respiratory symptoms. The analysis showed that the increase of air particle concentrations was negatively associated with the variability in PEFR. We estimated that the IQR increment of $PM_{10}$ or $PM_{2.5}$ were associated with 1.5 l/min (95% Confidence intervals -3.1, 0.1) and 0.8 l/min(95% CI -1.8, 0.1) decline in PEFR. Even though this study showed negative findings on the relationship between respiratory function and air particles, it was worth noting that the findings must be interpreted cautiously because exposure measurement based on monitoring of ambient air likely resulted in misclassification of true exposure levels and this was the first Korean study that $PM_{2.5}$ measurement was applied as an index of air quality.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1717-1725
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• 2000

The objective was to determine the relationship between feed intake levels and patterns, and growth performance and body composition of barrows and gilts using automatic feed intake recording equipment (F.I.R.E.). This system records the time of visits to the feeder and the duration and size of meals for individual animals housed in groups. Ninety-six crossbred pigs were grown from $33.4{\pm}0.51$ to $109.7{\pm}1.39kg$ live weight over a 13-week period. Eight mixed-sex groups of 12 pigs were used and 4 dietary treatments were compared giving 2 pens per treatment. The dietary treatments consisted of corn-soybean meal diets with differing protein levels which ranged from 14.7% to 19% between 30 to 55 kg, from 13.3% to 16.9% between 56 and 85 kg, and from 12.3% to 16.8% for the remainder of the study. Animals were ultrasonically scanned to measure loin-eye area and backfat thickness to estimate carcass fat-free lean content at the beginning and end of the study. Barrows had higher daily feed intake than gilts (2.67 vs. 2.46 kg resp. p<0.05) which was the result of a longer feeder occupation time per visit (4.77 vs. 4.54 min, resp. p<0.05), higher feed consumption rates (30.4 vs. 29.0 g/min, resp. p<0.05), and higher feed intakes per visit (136.9 vs. 126.8 g, resp. p<0.01). Gilts had less backfat and greater loin-eye area than barrows (p<0.05). Diet had no significant effect on growth performance and had limited impact on feeding patterns. Body weight showed high correlations with ADG (r=0.74), feed intake per visit (r=0.51) and feed consumption rate (r=0.69). Positive correlation were also found between daily feed intake and feed intake per visit (r=0.45), feeder occupation time per day (r=0.56), and feed consumption rate (r=0.55), and between daily feed intake and backfat thickness (r=0.32) and feed consumption rate and loin-eye area (r=0.32). There were negative correlations between number of feeder visit per day and daily feed intake (r=-0.54), and between feed intake per visit and number of feeder visits per day (r=-0.43). However, correlations between feed intake traits and carcass traits were generally low. Visits to the feeder were greatest during the morning (0700 to 1100 h) and lowest during the evening and nighttime. These results highlight limited variation among the sexes in feeding patterns and suggest important relationships between feeding behavior and feed intake.