• Title/Summary/Keyword: mixed sugars

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Production of DagA, a ${\beta}$-Agarase, by Streptomyces lividans in Glucose Medium or Mixed-Sugar Medium Simulating Microalgae Hydrolysate

  • Park, Juyi;Hong, Soon-Kwang;Chang, Yong Keun
    • Journal of Microbiology and Biotechnology
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    • v.24 no.12
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    • pp.1622-1628
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    • 2014
  • DagA, a ${\beta}$-agarase, was produced by cultivating a recombinant Streptomyces lividans in a glucose medium or a mixed-sugar medium simulating microalgae hydrolysate. The optimum composition of the glucose medium was identified as 25 g/l glucose, 10 g/l yeast extract, and $5g/l\;MgCl_2{\cdot}6H_2O$. With this, a DagA activity of 7.26 U/ml could be obtained. When a mixed-sugar medium containing 25 g/l of sugars was used, a DagA activity of 4.81 U/ml was obtained with very low substrate utilization efficiency owing to the catabolic repression of glucose against the other sugars. When glucose and galactose were removed from the medium, an unexpectedly high DagA activity of about 8.7 U/ml was obtained, even though a smaller amount of sugars was used. It is recommended for better substrate utilization and process economics that glucose and galactose be eliminated from the medium, by being consumed by some other useful applications, before the production of DagA.

The Changes in Quality of Sugars Chestnuts by Sugars and Heating Methods (당의 종류 및 가열방법에 따른 당침밤 제품의 품질 변화)

  • 홍순갑;황태영
    • Food Science and Preservation
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    • v.4 no.2
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    • pp.173-180
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    • 1997
  • The effects of sugars and heating methods on the sugar infilteration, hardness, color and organoleptic characteristics were investigated for quality improvement of sugared chestnuts. Among the tested sugars, isomaltooligosugar was the most effective on the rate of sugar infilteration. High temperature increased the rate of sugar infilteration during sugaring process, but color and flavor were deteriorated at 9$0^{\circ}C$. The most suitable temperature for sugaring process was 7$0^{\circ}C$. The product sugared with fructooligosugar recoreded the highest score in hardness, odor and preference than any other sugars tested and increased the rate of sugar infilteration when mixed with sugar at same amount. The changes of soluble solids in chestnuts boiled with microwave oven were ranged from 18。Brix to 32。Brix, while chestnuts heated in general were from 18。Brix to 28。Brix. Chestnuts boiled with microwave heating were sugared rapidly. The hardness of boiled and sugared chestnuts was lower when treated with microwave than with general heating. Hunter's L and b value of sugared chestnut treated with microwave decreased during processing but a value somewhat increased.

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Study on Improvement of Kraft Paper sack Mixed with Recycled Paper for Sugars and Assorted Feeds (설탕 및 사료의 파지혼용 크라프트 지대 개선에 관한 연구)

  • Lee, Soo-Keun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.5 no.2
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    • pp.38-41
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    • 1999
  • This study was carried out to investigate improvement of kraft paper sack mixed with recycled paper for sugars and assorted feeds. Four Plies of kraft paper mixed with recycled paper(basis weight : $80g/m^2$) were taken place of two plies of kraft paper made from all pulp(basis weight : $98g/m^2$) and one ply of kraft paper mixed with recycled paper(basis weight : $80g/m^2$) for sugar 30kg. Three Plies of kraft paper mixed with recycled paper(basis weight : $80g/m^2$) were taken place of two plies of kraft paper made from all pulp(basis weight $98g/m^2$) for sugar 15kg and assorted feed 25kg. Physical properties, tensile strength, internal tearing strength and bursting strength, were improved by exchanging kraft paper mixed with recycled paper for kraft paper from all pulp and so kraft paper sacks made from all pulp were no damage in handling. They had so high friction coefficient that there was no danger of collapse on pallet by slipperiness in transportation and handling. And they had high printability so disappearance of printing by the contact with other paper sack in transportation was not found.

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Changes on the Components of Free Polysaccharide from Cell Wall of Persimmon Fruit by Treatments of Cell Wall Degrading Enzymes (세포벽분해효소의 처리에 따른 감과실의 세포벽 유리 다당류의 변화)

  • 신승렬;김미현
    • Food Science and Preservation
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    • v.2 no.1
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    • pp.173-183
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    • 1995
  • This paper was carried out to investigate changes in chromatograms of polysacctatides and soluble pectins on Sephadex G-50 and non-cellulosic neutral sugars of polysaccharides isolated from cell wall of persimmon fruits treated with polygalacturonase and $\beta$-galactosidase in vitro. The chromatogram pattern of soluble pectins extracted from cell wall treated with $\beta$-galactosidase on Sephacryl S-500 column were similar to those of untreatment, but contents of soluble pectins treated with $\beta$-galactosidase were different from those of untreatment. The patterns of chromatograms In soluble pectins extracted from cell wall treated with polygalacturonase were more complex and lower molecular polymer than those of other cell wall-degrading enzyme treatments. Non-cellulosic neutral sugar of polysaccharides in fraction I of soluble material treated with polygalacturonase was rhamnose, those in fraction II were similar to those in fraction III and contents of arabinose, xylose and glucose were higher than contents of other non-cellulosic neutral sugars. Non-cellulosic neutral sugars of polysaccharides in fraction I in soluble material by $\beta$-galactosidase treatment were rhamnose, arabinose, galactose and mannose. Content of glucose of polysaccharides in fraction II was higher than that in fraction I . Non-cellulosic neutral sugars treated with mixed enzyme were rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose. Compositions of non-cellulosic neutral sugars of polysaccharides in fraction I were similar to those in fraction II and III.

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Evaluation of Secondary Acid and Enzymatic Hydrolysis of Hemicellulose in Hot Water Pre-Pulping Extract of Mixed Hardwoods

  • Um, Byung-Hwan
    • Journal of the Korean Wood Science and Technology
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    • v.40 no.2
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    • pp.123-132
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    • 2012
  • Pre-pulping extracts were found to contain a dilute amount of xylo-oligosaccharides and acetic acid as the major components, and many minor components including other organic acids, lignin-derived phenolics, and sugar degradation products. Once separated from the pulp, a secondary hydrolysis step was required to hydrolyze oligomeric hemicellulose sugars into monomeric sugars before fermentation. The following study detailed the extent of hemicellulose recovery by pre-pulping using hot water extraction and characterized the hydrolysis of the extract with respect to comparing acid and enzymatic hydrolysis. The secondaryhydrolysis of hot water extracts made at an H-Factor of 800 was tested for a variety of acid and enzyme loading levels using the sulfuric acid and xylanases. The maximum fermentable sugar yield from acid and enzyme hydrolysis of the extract was 18.7 g/${\ell}$ and 17.7 g/${\ell}$ representing 84.6% and 80.1% of the maximum possible yield, respectively.

Fermentation of Glucose, Xylose and Cellobiose by Pichia stipitis (Pichia stipitis에 의한 Glucose, Xylose 및 Cellobiose의 발효)

  • 이유석;권윤중;변유량
    • Microbiology and Biotechnology Letters
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    • v.20 no.1
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    • pp.91-95
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    • 1992
  • The hydrolyzates of lignocellulosic biomass contain a mixture of glucose, xylose and cellobiose. The yeast which can produce ethanol efficiently from xylose and cellobiose was selected and its growth and ethanol formation behavior on each sugar and their mixture were investigated. Ethanol yields during batch culture of Pichia stipitis CBS 5776 were 0.4. 0.36 and 0.23 g/g substrate on glucose, xylose and cellobiose, respectively. Mixed sugar fermentation data indicate that glucose causes catabolite regulation on xylose and cellobiose utilization. However, xylose and cellobiose were utilized simultaneously. Ethanol yields on mixtures of sugars were generally additive for each of the substrates.

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Flavor Modification of Mideoduck (Styela clava) Drips by Maillard Reaction (Maillard 반응에 의한 미더덕 체액의 풍미개선)

  • Kang, Seok-Joong;Jung, Sung-Ju;Choi, Yeung-Joon;Choi, Byeong-Dae
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1829-1837
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    • 2010
  • Mideoduck drips were mixed with amino acids (Met, Tau, Gly, Ala, Thr, Cys), thiamine and sugars (Glucose, Ribose) for flavor modification and evaluation using the Maillard reaction. To mask the seafood flavor, onions, spring onions, garlic, ginger, citric orange and green tea were mixed with Mideoduck drips at $160^{\circ}C$ for 2.5 hr in a stainless still reaction bomb. The glucose/thiamine model reaction system was estimated to be lower than the ribose/thiamine model system, and an extreme case is the ribose/Met model system. Mixed system of glucose, ribose and taurine containing sulfur compounds showed fair results. Among the Mideoduck drips mixed with sugars and amino groups, only thiamine model systems were estimated to be normal. The flavor composition of Mideoduck drips/sugars model system, and long chain fatty acids were composed of 31.32~62.71% total flavor content. The 1,2-benzenedicarboxylic acid dibutylester contents made up more than 20% of the model system in groups A, B and C. From the model system in this study, drip/glucose, drip/ribose, drip/glucose/citric orange, and drip/glucose/glycine/cystine groups showed most intense good flavor.

Physicochemical Characteristics of $Consomm{\acute{e}}$ Prepared with Different Ratios of Beef and Chicken (쇠고기와 닭고기의 배합비에 따른 $Consomm{\acute{e}}$의 이화학적 특성)

  • Kim, Young-Sik;Jang, Myung-Sook
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.5
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    • pp.564-572
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    • 2006
  • The application of Consomm prepared with different ingredients of ground beef and ground chicken ingredients to improve the taste preferences and reduce saving the food cost of Consomm was attempted by reviewing fundamental data and its examining the effect on the physicochemical characteristics of treatment. These treatments were prepared with the following different ratios (9:1, 7:3, 5:5, 3:7, 1:9 ; w/w) of ground beef to and ground chicken: 9:1, 7:3, 5:5, 3:7, and 1:9, w/w, respectively. Gelatin contents were increased significantly with by increasing the mixed ratio of ground chicken. The free sugars identified from Consomm were, glucose, fructose, and sucrose. Free sugar contents, increased significantly by with increasing the mixed ratio of ground beef. (Ed- what are the highlighted treatments? You need to explain in this abstract otherwise the reader doesn't know. For simplicity I suggest you don't use such nomenclaturehere in the abstract and simplify with merely the weight ratios. Confirm the changes that I've made) The highest and lowest free sugars werewas shown at A9:1 and 1:9 treatments, respectively whereas the lowest value was shown at I treatment. As many as 20 different kinds of free amino acids were detected infrom the Consomm and the amount of total amino acids waswere increased with by increasing the mixed ratio of ground chicken. The contents of arginine, glutamic acid and alanine contents were high in the free amino acids of Cconsomm prepared with different ingredients. The In the changes of mineral contents in the Consomm prepared with different ingredients, the mineral showed high contents increased according to in the following order of: K, Na, P, Mg, Ca, Fe, and Zn. From the The results of analysis results, in the Relatin contents, free sugars, free amino acids and mineral contents were all increased with by increasing the mixed ratio of ground chicken. The 7:3 treatment showed From above results, it was showed that B treatment prepared with a ratio of 1 ground beef to 3 ground chicken was the bestmost preferred in physicochemical qualities.

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Succinic Acid Production by Anaerobiospirillum succiniciproducens ATCC 29305 Growing on Galactose, Galactose/Glucose, and Galactose/Lactose

  • Lee, Pyung-Cheon;Lee, Sang-Yup;Chan, Ho-Nam
    • Journal of Microbiology and Biotechnology
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    • v.18 no.11
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    • pp.1792-1796
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    • 2008
  • Succinic acid-producing Anaerobinspirillum succiniciproducens was anaerobically grown on galactose, galactose/glucose, or galactose/lactose in order to study its galactose fermentation. Unlike a previous report, A. succiniciproducens was found to efficiently metabolize galactose as the sole carbon source at a rate of 2.4 g/g-DCW/h and produced succinic acid with as high a yield of 87% as with using glucose. When glucose and galactose were present, A. succiniciproducens metabolized both sugars simultaneously. Furthermore, when lactose and galactose coexisted, lactose did not inhibit the galactose fermentation of A. succiniciproducens. Therefore, co-utilization of galactose and other sugars can improve the productivity and economy of bio-based succinic acid processes.

Cellulose Utilization and Protein Productivity of Some Cellulolytic Fungal Co-cultures

  • Eyini, M.;Babitha, S.;Lee, Min-Woong
    • Mycobiology
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    • v.30 no.3
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    • pp.166-169
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    • 2002
  • Protein productivity by the cellulolytic fungi, Trichoderma viride(MTCC 800), Chaetomium globosum and Aspergillus terreus was compared in co-culture and mixed culture fermentations of cashewnut bran. Co-cultures were more effective in substrate saccharification, which ranged between $85{\sim}88%$ compared to the $62{\sim}67%$ saccharification shown by the monocultures. Maximum saccharification was induced by T. viride and C. globosum co-culture resulting in the highest 34% release of reducing sugars. The maximum 16.4% biomass protein and the highest protein productivity(0.58%) were shown by T. viride and A. terreus co-culture. A. terreus performed better in co-culture in the presence of T. viride rather than with C. globosum. Among the cellulolytic enzymes, FPase(Filter Paper Cellulase) activity was significantly higher in all the co-cultures and in the mixed culture than in their respective monocultures. Mixed culture fermentation involving all the three fungi was not effective in increasing the per cent saccharification or the biomass protein content over the co-cultures.