• Korean Journal of Microbiology
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• v.50 no.4
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• pp.345-350
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• 2014

The aim of this study was to investigate the effect of high temperature on the viability of probiotic organisms (Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) mixed with animal feed under controlled conditions by simulating a farm feed bin in the summer. Following inoculation of probiotics into the feed, the pH and probiotic viability were monitored during an 8-day incubation at room temperature. Sterile and non-sterile feeds displayed different patterns of pH changes, with increased pH in non-sterile feed at 2 days, but a pattern of decreasing pH at 4 days. The viabilities of S. cerevisiae and B. subtilis after mono/co-inoculation were maintained without substantial changes during the incubation, whereas L. plantarum viability tended to decline. In both non-sterile and sterile feeds, the probiotics were maintained or grew without any antagonistic effects. Probiotic viability was also tested upon a shift to high temperature ($60^{\circ}C$). There was no distinct change in pH between sterile and non-sterile feeds after the temperature shift. L. plantarum and S. cerevisiae could not survive at the high temperature, whereas B. subtilis displayed normal growth, and it inhibited the growth of contaminant microbes. Fungal growth was not observed in non-sterile feed 2 days after supplementation with B. subtilis. Therefore, heat resistant B. subtilis could be safely used in feed bins to inhibit microbial contamination, even at high temperatures. The prevention of elevated temperature in feed bins is necessary for the utilization of L. plantarum and S. cerevisiae during the summer season.

• Food Science and Preservation
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• v.23 no.3
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• pp.326-334
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• 2016

Omija (Schizandra chinensis) extract (OE) was fermented by using Lactobacillus plantarum EJ2014 to produce a beverage fortified with gamma-aminobutyric acid (GABA). After 2 days of fermentation in the presence of 2% monosodium glutamate (MSG) and 0.5% yeast extract (YE), the four-fold-diluted OE showed a higher viable cell count ($2.2{\times}10^9CFU/mL$) and lower acidity (1.2%) than that of the unfermented OE. In particular, addition of MSG as a precursor resulted in a small increase in the initial pH. MSG (2%) was completely converted to GABA (0.92%) during lactic acid bacteria fermentation for 3 days. Furthermore, the acidity of the fermented OE decreased from 1.74% to 0.56%. In addition, the original red color of the OE disappeared during LAB fermentation. However, when the fermented OE was mixed with 50% of the original OE, the original red color was recovered, with 19.56 and 13.92 for Hunter L and a values, respectively. The mixture of 50% original OE and 50% fermented OE showed the highest sensory score including the highest overall preference. In conclusion, the OE fortified with GABA and probiotics was produced by fermentation with a static culture, L. plantarum EJ2014.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2001.11a
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• pp.61-63
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• 2001

Two experiments were conducted to investigate the feeding influence of wood vinegar and mixed with probiotics on performance and physiological related of broiler chicks for five weeks. Diets contained 21.5%, 3,100kcal/kg and 19.0% CP, 3,100kcal/kg ME for starting and finishing period, respectively. In Expt 1, Four levels of wood vinegar(0, 0.05, 0.10, 0.20%) were fed into two hundred forty chicks with four replicates. In Expt 2, 0, 0.1% wood vinegar(W), 0, 0.1% lactobacillus casei(L) and bacillus subtilis (B) were applied to 2${\times}$2${\times}$2 facotrial design for eight treatments with four replicates. Weight gain, feed intake, feed cholesterol were detected at five weeks old in each experiment. In Expt 1, Weight gain of chicks fed 0.1% wood vinegar(WV) was increased upto 1645.8g, but was not significantly different. However, chicks fed 0.2% WV showed the highest gain and siginificantly different compared to that of control (P＜0.05) for finishing period. Feed intake also showed similar tendency with weight gain. There were no statistical difference in feed conversion. Abdominal fat seemed to be decreased in WV supplemental groups, whereas ND antibody titer tended to increase in those treatments. In Expt 2, 0, 0.1% WV, lactobacillus, bacillus were combined with each other(W$\sub$0/L$\sub$0/B$\sub$0/, W$\sub$0/L$\sub$1/B$\sub$0/, W$\sub$0/L$\sub$0/B$\sub$1/, W$\sub$0/L$\sub$1/B$\sub$1/, W$\sub$1/L$\sub$0/B$\sub$0/, W$\sub$1/L$\sub$1/B$\sub$0/, W$\sub$1/L$\sub$1/ B$\sub$0/, W$\sub$1/L$\sub$0/B$\sub$1/, W$\sub$1/L$\sub$1/B$\sub$1). WV supplemental groups significantly improved feed conversion of all treatments(P＜0.05). There were no interaction in weight gain and feed intake. Total cholesterol was signficantly decreased in WV plus lactobacillus and ladtobacillus plus bacillus treatments. Liver to body ratio(%) of lactobacillus plus WV significantly decreased compared to that of other treatments. The results of these experiments showed that wood vinegar tended to improve the performance of broiler chicks, but was not effect in its combination with lactobacillus or bacillus treatments.

• Journal of Life Science
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• v.20 no.11
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• pp.1729-1737
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• 2010

This study was conducted to establish the optimal medium condition for the animal probiotic Lactobacillus plantarum by using onion juice. Cell yield and antioxidant activity increased in proportion to high additive levels of onion juice in medium. Onion juice, sucrose and yeast extract were selected as media ingredient factors and the effects of their mixed ratio in medium were evaluated. The full factorial design consisted of 24 experimental runs and was employed to estimate the main effects of the factors and their interactions. Significant positive effects on cell yield and antioxidant activity was shown with yeast extract and onion juice, respectively. Significant interaction was found only between sucrose and yeast extract in antioxidant activity. Finally, we selected an optimal medium that was composed of (g/l) onion juice, 600; sucrose, 15; yeast extract, 5. The efficiency of this optimum medium was estimated by using a 5 l jar fermenter. As a result, the maximum cell yield was $9.7\;{\log}_{10}$ (CFU/ml) at 12 hr. Cell yield at the end of incubation (20 hr) was $8.9\;{\log}_{10}$ (CFU/ml) and it was very similar with the predicted value, $9.0\;{\log}_{10}$ (CFU/ml). Antioxidant activity of culture was maintained at about 60~65% during all incubation time, resulting in a higher-than-predicted activity of 47.1%.

• Journal of agriculture & life science
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• v.45 no.2
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• pp.93-101
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• 2011

This study was conducted to investigate the effect of probiotic from pine needle microbes on growth performance, blood characteristics, carcass traits and economy of pigs. One hundred eighty pigs (L${\times}$Y${\times}$D, average body weight=75 kg) were grouped and housed with 15 animals per pen for 3 replications per each treatment. The ingredients and pine needle microbes mixed and fermented on anaerobic condition for 10 days to make the fermented diet. The basal diet was substituted with 0 (CON), 3 (T1), 5 (T2) and 10% (T3) of fermented diet, the pigs were fed for 42 days and slaughtered at the end of the trial to measure the carcass traits. Crude protein concentration of fermented diet on 10 day was higher (p<0.05) than that of on 0 day, while crude fiber concentration and pH were lower (p<0.05). The supplementation of fermented diet did not affect on growth performance and feed intake. Hemoglobin concentration of whole blood in T3 was higher (p<0.05) than that of CON, and hematocrit concentrations in CON and T1 were higher (p<0.05) than that of T2 and T3. The concentration of platelet was higher (p<0.05) in T1 than in T2 and T3. The CON had higher (p<0.05) total cholesterol, LDL-cholesterol and triglycerides concentrations than the treatments, whereas lower (p<0.05) HDL-cholesterol concentration. Carcass weight and dressing in T1 and T3 were higher (p<0.05) than those of CON and T2. The backfat thickness was higher (p<0.05) in CON and T2 than in T3. The carcass grade and economy improved by supplementation of fermented diet compare to CON. In conclusion, the supplementation of fermented diet using probiotics from pine needle microbes could improve health and meat quality and reduce the feed cost of pigs.

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.552-558
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• 2021

Traditional foods are manufactured according to the characteristics of each region for the nations of the world. Korea has mainly farmed, and seasonings have developed around rice and vegetables. In fermented foods, lactic acid bacteria such as Lactobacillus sp. and Pediococcus sp. and Bacillus sp. were isolated and identified from fermented foods. In this study, lactic acid bacteria were isolated and identified from commercially available traditional Korean fermented foods, and candidate strains were selected through antibacterial activity tests on human and fish disease bacteria. Thereafter, the final strain was selected by examining the resistance to simulated gastric and intestinal fluids, and hemolysis. The three (M1, K1, C13) final selected latic acid bacteria were miced with prebiotics and the antibacterial activity of synbiotics was evaluated. As for the fist antibacterial activity result, C13 showed high antibacterial acitivity in human diseases and fish diseases. Then, M1, K1 and C13, which did not produce β-haemolysis and were resistant to simulated gastric and intestinal fluids, were subjected to the second antibacterial activity of synbiotics. When the three prebiotics (FOS, GOS, Inulin) and probiotics (M1, K1, C13) were mixed, the antibacterial activity was increased or inhibited. Based on the 16S rRNA gene sequencing results, K1 and M1 were analyzed as Bacillus tequiensis 99.72%, Bacillus subtilis 99.65%, Bacillus inaquosorum 99.72%, Bacillus cabrialesii 99.72%, Bacillus stercoris 99.58%, Bacillus spizizenii 99.58%, Bacillus halotolerans 99.58%, and Bacillus mojavensis 99.51%. And C13 was analyzed as Bacillus velezensis 99.71%, Bacillus nematocida 99.36%, Bacillus amyloliquefaciens 99.44%, Bacillus atrophaeus 99.22%, and Bacillus nakamurai 99.44%.

• Journal of Animal Science and Technology
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• v.55 no.4
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• pp.273-280
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• 2013

This study was conducted to investigate the effects of fermented king oyster mushroom by-products diet (FMBD) on the growth performance, blood characteristics, and carcass traits of fattening pigs and its economics. The fermented diet mainly contained 40.0% king oyster mushroom (Pleurotus eryngii) by-products, 20.0% corn, 28.0% soybean meal, 0.1% supplemental probiotics and 0.08% cellulase. The mixed ingredients were fermented for 1 d at $37^{\circ}C$ followed by 9 d at room temperature, after which they were dried at $45^{\circ}C$ for 3 d. Pigs (n=96) were divided into eight heads per pen, four diet treatments and three replications. The basal diet (C) was substituted with 20% (T1), 50% (T2) and 80% (T3) FMBD. The concentration of crude protein (CP) was significantly higher (p<0.05) at the end of the fermentation period. The average daily gain (ADG) and feed efficiency were significantly lower (p<0.05) in T3 than C. Additionally, carcass grade was significantly better (p<0.05) in all treatments than C and the ratio of high carcass grade was higher. Although substitution of FMBD decreased growth performance, it improved carcass grade and decreased the feed cost of fattening pigs. Therefore, it was expected that the increase in the utilization ratio of FMBD will reduce the cost of animal production.

• Journal of Dairy Science and Biotechnology
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• v.35 no.1
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• pp.25-31
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• 2017

This study was conducted to find means to reduce the foul smell emitted from chicken manure. Rice water was inoculated with Lactobacillus sakei and then examined to determine the bacterial survival rates during storage, and whether fermentation had occurred. Rice water was an excellent medium for the growth of L sakei, given that a viable cell count was maintained for 15 days during storage at $4^{\circ}C$ and decreased slightly thereafter. Furthermore, microorganisms in chicken manure were separated and 14 species were identified. The antibacterial activity of an L. sakei supernatant against the identified microorganisms was measured using the agar diffusion method. The growth of 11 out of 14 species was inhibited, and only Corynebacterium variabile, Enterococcus faecium, and Raoultella ornithinolytica survived. Rice water was fermented by inoculation with L. sakei and mixed with chicken manure, and the quantities of ammonia, ethyl mercaptan, and hydrogen sulfide were measured after 48 hours. Emissions of ammonia and ethyl mercaptan were reduced significantly.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1163-1170
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• 2015

Two probiotic strains, Lactobacillus johnsonii x-1d-2 and Lactobacillus mucosae x-4w-1, originally isolated from piglet feces, have been demonstrated to possess antimicrobial activities, antibiotic resistances and interleukin-6 induction ability in RAW 267.4 macrophages in our previous study. These characteristics make L. johnsonii x-1d-2 and L. mucosae x-4w-1 good candidates for application in feed probiotics. In this study, soybeal meal, molasses and sodium acetate were selected to optimize the growth medium for cultivation of L. johnsonii x-1d-2 and L. mucosae x-4w-1. These two strains were then freeze-dried and mixed into the basal diet to feed the weaned piglets. The effects of L. johnsonii x-1d-2 and L. mucosae x-4w-1 on the growth performance and fecal microflora of weaned piglets were investigated. The results showed that the bacterial numbers of L. johnsonii x-1d-2 and L. mucosae x-4w-1 reached a maximum of 8.90 and 9.30 log CFU/mL, respectively, when growing in optimal medium consisting of 5.5% (wt/vol) soybean meal, 1.0% (wt/vol) molasses and 1.0% (wt/vol) sodium acetate. The medium cost was 96% lower than the commercial de Man, Rogosa and Sharpe medium. In a further feeding study, the weaned piglets fed basal diet supplemented with freeze-dried probiotic cultures exhibited higher (p<0.05) body weight gain, feed intake, and gain/feed ratio than weaned piglets fed basal diet. Probiotic feeding also increased the numbers of lactobacilli and decreased the numbers of E. coli in the feces of weaned piglets. This study demonstrates that L. johnsonii x-1d-2 and L. mucosae x-4w-1 have high potential to be used as feed additives in the pig industry.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.4
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• pp.114-119
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• 2003

The fermentative conversion of food wastes into probiotic feed was investigated by seeding of mixed inoculum of Lactobacillus acidophilus and Saccharomyces cerevisiae. After grinding finely, optimal fermentation conditions for aeration was investigated at $30^{\circ}C$, The viable cell count of lactic acid bacteria and yeast during fermentation were monitored by controlling aeration rate at each different aeration degree of 0v.v.m 0rpm, 0.25v.v.m 100rpm, 0.5v.v.m 200rpm, and 1v.v.m 500rpm respectively. The most active growth of the yeast was shown at 0.5v.v.m 200rpm as $4.5{\times}10^9CFU/m{\ell}$. By controlling aeration rate, the pH of the probiotics feed could be controlled between 4-5 for the enhancement of preservation characteristics and acceptability for cattle feeding.