• Korean Journal of Soil Science and Fertilizer
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• v.20 no.3
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• pp.209-216
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• 1987

The experiments were carried out in lab. as well as in pots, to develop the agricultural usage of water swellable polymer, a kind of polyacrylic acid(K-sorb) synthesized by the Korea Advanced Institute of Science & Technology (KAIST) recently. The changes of soil physical properties and the influences to crops were investigated with various levels of K-sorb. When the K-sorb mixed with soils and soaked up distilled water, the volume of soils increased with the increase of soil available water contents and increase of K-sorb application levels. The rate increase of soil available water was higher in the coarse textured soils than in the fine while the swelling rate of soil volume showed adverse tendencies. A positive linear regression was observed between the contents of available soil water and levels of K-sorb. K-sorb application decreased bulk density and hardness due to the increase of porosity after soybean cultivation. The permeability in coarser textured soils such as sandy and coarse loamy families was decreased with the increase of K-sorb but in the medium textured soils it was opposite. At higher levels of K-sorb, about 0.5%, the permeability abruptly decreased due to dispersion and vertical movement in silty soils, while it was not changed in fine clayey soils but has the same trend with silty soils. In the plot of 0.3% of K-sorb application, the growth of soybean such as number of pods and stem length etc. increased and the yield also increased about 1.2-1.8 times of control. The optimum amounts of K-sorb were slightly different according to soil texture but estimated from regression curves were about 0.2% to 0.35% of soils in dry weight bases.

• Journal of Chest Surgery
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• v.35 no.3
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• pp.171-176
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• 2002

Background: Blood-foreign interaction cause activation of coagulation and inflammatory process that may lead to multiorgan dysfunction and determine the surgical outcomes. Of the methods for assessing the biocompatibility, the platelet adhesion study is considered as the most valuable evaluation step in blood-foreign interaction. As the most studies have used in-vitro or ex-vivo conditions, we have developed a technique of quantification for platelet adhesion on the blood contact surface by using in-vivo injection of radioactive platelets. Material and Method: A coupled bypass circuit was designed to connect the proximal and descending thoracic aorta in 6 piglets(20∼25 Kg). One side of the circuit tube was consisted of a heparin coated PVC tube(10mm in ID, n=6, Experimental group), and the other, a non-heparin coated PVC tube(10mm in ID, n=6, Control group). After cannulation, the blood was circulated through the circuit for 2 hours. Platelet concentrate was prepared from homologous pig blood 24 hours before the experiment. The platelet concentrate was incubated with Tc-99m-HMPAO for 30 min and then centrifuged for 10 min. The supernatant was discarded and the radio-labeling efficacy was measured. The radio-labeled platelet concentrate was mixed with the autologous plasma to make the volume 5 ml, and the mixture was injected intravenously into the experimental animal. After 2 hour circulation, 5 pieces of the specimen(10mm in length each) were obtained from each PVC tube. The radioisotopes were counted with a gamma counter(Cobra ll, Packard, USA), and the ratio of radioisotope count was compared between the control and experimental group. Result: The radioisotope count number was 537.3221.1 Ci/min in the control group and 311.1 184.5 Ci/min in the experimental group(p=0.0104). The ratio between the groups was 1 to 0.58 (p=0.004). Conclusion: In vivo quantification using technetium-99m-HMPAO labeled platelets is simple and reproducible in evaluating platelet adhesion on a foreign surface. We suggest this technique to be a useful tool for blood compatibility test.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.9 no.2
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• pp.162-168
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• 2006

Purpose: Microbial colonization of the intestine begins just after birth and development of the normal flora is a gradual process. The first bacteria colonizing the intestine in newborns are Staphylococcus, Enterobacteriaceae and Streptococcus. For several days after birth, the number of Bifidobacterium spp. increase. The aim of this study was to investigate the changes of microflora for seven days postnatally in neonatal stool. Methods: Fifteen neonates (breast : formula : mixed feeding 1 : 8 : 6, vaginal delivery : cesarean section 3 : 12) who were born at the Kangdong Sacred Heart Hospital, Hallym University were enrolled. First meconium and stools of postnatal 1-, 3-, and 7-day were innoculated. Blood agar plates for total aerobes, trypton bile X-glucuronide agar for E. coli, phenylethyl alcohol agar for gram positive anaerobes, MRS agar for Lactobacillus spp., bifidobacterium selective agar for Bifidobacterium spp. and cefoxitin-cycloserine-fructose agar for Clostridium difficile were used in the general incubator ($CO_2$ free incubator), $CO_2$ incubator or the anaerobic chamber for 48 or 72 hours at $37^{\circ}C$ and then colony forming units were counted. Results: No microflora was identified in the first meconium. Total aerobes, E. coli, and gram positive anaerobes were significantly increased with advancing postnatal days. In only one baby, Lactobacillus acidophilus was detected $2{\times}10^5CFU/g$ in the seven-day stool. Bifidobacterium spp. was detected in two babies. Clostridium difficile was not detected during the seven days. There were no significant differences in the bowel flora depending on the delivery pattern and feeding method. Conclusion: This study shows many changes in the intestinal normal flora in neonatal stool during seven days postnatally. If these findings are confirmed with larger studies, the data may be preliminary findings to support use of probiotics in neonates.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.3
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• pp.311-319
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• 2007

This study was conducted to estimate effects of application of controlled release complex fertilizer with latex coated urea (LCU-complex) on growth and grain quality of rice under direct seeded on dry paddy (DS) and transplanted on flooding paddy (TP). Three types of latex coated urea different nitrogen (N) releasing were LCU40, LCU80 and LCU100. The time of N releasing of LCU formulations in water at both 20 and $30^{\circ}C$ was faster in the order of LCU40, LCU80, LCU blend (LCU40, LCU80 and LCU100 was mixed in ratio of 2:2:1), and LCU100. The number of tillers and dry matter weight were great in order of LCU-complex 100% > LCU-complex80% > urea and plant height was not significant. Grain yields at LCU-complex80% in both DS and TP plot were similar to those of urea application. N recovery of LCU-complex80% and 100% was improved 8 and 6% compared to that of conventional urea split application in DS plot and 9 and 4% in TP. Content of protein of grain at applied LCU-complex was less 0.8% and $0.1{\sim}0.7%$ than that of urea in DS and TP, respectively. Content of amylose and Mg/K ratio in rice grain was not significant. Consequently application of LCU-complex blended types of coated urea different N releasing can be reduced 20% of N without yield reduction and improved grain quality compared with urea application.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.813-818
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• 2003

This study was carried out to survey operation system of self-making TMR and particle size of self-making and commercial TMR, then to compare TMR particle size recommended by Penn State Particle Size Separator. Daily mixing time in self-making TMR averaged 48.6${\pm}$28.0min., 2 augar type of mixer was most by 55.5% and daily 2 times of mixing in summer and other season was 44.4 and 22.2%, respectively. Percentage of residual feed in upper sieve(19mm diameter), middle sieve(8 to 18mm diameter) and lower pen was 21.5~25.6%, 16.0~25.8%, 52.8~61.4%, respectively, at self-making TMR mixer. Percentage of particle sizes of more than 19mm in self-making and commercial TMR was 24.9${\pm}$1.4 and 26.2${\pm}$1.7%, that of 8-10mm 22.8${\pm}$1.0 and 12.8${\pm}$1.2 and that of less than 8mm 52.3${\pm}$1.7 and 61.0${\pm}$1.5, respectively, which in particle length of 8~13mm in self-making and commercial TMR was lower by 82.6 and 100% compared to that recommended by Penn State particle separator, respectively. Especially there was not particle length of more than 19mm in commercial TMR at all. It is necessary to check the number of cows ruminating in a farm in order to estimate the particle size of TMR; it is recommended to change the TMR mixing time or the TMR formulae if the proportion of ruminating cows in a farm is less than 40 %.

• Korean Journal of Environment and Ecology
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• v.25 no.3
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• pp.358-364
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• 2011

To examine the possibility of natural regeneration of six Acer species in broadleaved mixed forest in Korean peninsula, samples of six Acer species' samaras were collected at several seed sources from September to October 2009 and 2010, and seed morphological characteristics and viability were studied using cutting method. Inflorescense type of Acer mandshuricum(AM), A. pseudosieboldianum(APS) and A. palmatum(AP) is corymb, that of A. pictum subsp. mono(APSM) and A. okamotoanum(AO) is flat-topped panicle, and that of A. ukurunduense(AU) is raceme. Number of seed-set per inflorescense proves the highest value 38.5 sets in AU and the lowest value 2.4 sets in APS. Diameter of the seeds proves highest value 13.5mm in AO, and the lowest value 4.7mm in APS. Angles between the wings proves the highest values $130.05^{\circ}$ in APS, and the lowest value $48.60^{\circ}$ in AU. Air dry weight of 20 seed-sets proves highest value 3,900mg, in AO, and the lowest value 404mg in AU. Viable seed ratio of AP proves 50%, and that of AU does 43.2%. Those of other four species ranges 8.6~22.2%. Considering postdispersal seed predators and disturbance of litter, viable seeds of APSM, AO, AM, and APS supplied in the natural forest in Korea might be insufficient for seedling establishment. This study showed that sound viable seed supply might be key factors of natural regeneration of major Acer species in Korea. The openings made by insect pests were observed on the seed-coat of APSM, AM, AU, and APS samara, and Bradybatus sharpi were observed in the samaras of APSM and AO. Further study on the seed insect fauna, pre- and post-dispersal seed viability, and annual variation on these factor should be needed.

• The Korean Journal of Mycology
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• v.24 no.1 s.76
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• pp.38-48
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• 1996

The survival or colonization of beneficial organsisms and suppression of root rot of ginseng (Panax ginseng) by two distinct bacteria, Pseudomonas cepacia, Bacillus cereus and three mycorrhiza in pot soil were investigated and compared with uninoculated root. In separate inoculation, colonization of roots by P. cepacia was maintained at 6.25 (log cfu/g root) during growth for 10 days under pot culture conditions comparing to $5.62{\sim}6.19$ by mixed treatment with other organisms. Colonizations of P. cepacia were gradually decreased from 6.25 (log cfu/g root) in 10 days growth to 3.01 (log cfu/g root) in 270 days incubation period. This reduction was also investgated in combination treatments by B. cereus or F. solani. The numbers of Fusarium spp. were colonized high number in rhizosphere soil from 3.33 to 3.67 (log cfu/g root) in control within $10{\sim}60$days after treatment of pathogen F. solani, but it's numbers were markedly decreased in 270 days cultivation of plant from 3.33 to 1.02 (log cfu/g root) after treatment. In treatment of beneficial strains of P. cepacia and B. cereus, P. cepacia significantly suppressed the development of root rot from 4.3 in control to 1.2 in treatment, whereas B. cereus alone had no effect on the rate of disease suppression. The disease index $(1.8{\sim}2.3)$ in combination of two bacteria was reduced in plants inoculated with both P. cepacia and B. cereus comparing to the index (4.3) of control. As an effect of inoculation with mycorrhiza on disease suppression, suppression of root rot by F. solani was reduced to $1.2{\sim}1.6$ in disease index in treatment of Glomus albidum and Acaulospora longular comparing to 4.3 of control. In the treatment of bacterial strain P. cepacia and mycorrhizal fungus Glomus albidum, the disease suppression was apparent to 1.2 and 1.2 comparing to 4.3 of control in disease index respectively.

• Journal of Veterinary Clinics
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• v.28 no.3
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• pp.297-302
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• 2011

Avian pathogenic Escherichia coli (APEC) causes a number of extraintestinal diseases in poultry. A virulence factor, P-fimbriae is firmly associated with the diseases. In this study, to develop an effective vaccine for the prevention of APEC, recombinant attenuatted Salmonella Typhimurium vaccines expressing PapA and PapG of P-fimbriae were evaluated whether these induced protective immune responses in murine models. Female BALB/c mice were primed and boosted orally at 7 and 10 weeks of age. In all immunized mice, the antigen-specific serum IgG levels were remained higher than those in the control mice from the fourth week post inoculation till the end of this study. In addition, antigen-specific serum IgG levels in the prime-booster immunized mice were enhanced as compared to the single immunized mice among each immunized group. The antigen-specific mucosal IgA levels in the mice immunized with each strain also induced higher than those in control mice. In addition, serum IgG and fecal IgA levels in mice administered with the combination of both strains were highly induced compared to those in mice immunized with each strain alone. These results indicated that PapA and PapG worked together for inducing high immune responses. To partly discern the nature of immunity induced by the strains, we quantified serum IgG subtypes IgG1 and IgG2a specific to antigens. The PapA and PapG strains biased the immunity to the Th1-type, as determined by the IgG2a/IgG1 ratio. On the other hand, the immunization with the both strains in combination produced mixed Th1- and Th2-type immune responses. These indicated that immunization with the combination of PapA and PapG could elicit both humoral and cell-mediated immunities.

• Food Science of Animal Resources
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• v.23 no.3
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• pp.250-261
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• 2003

This experiment was carried out to examine the fermentation properties of yogurt with Lycii fructus, Lycii folium and Lycii cortex powder, and extract additives at concentrations of 0.5, 1.0, 2.0, 4.0, and 6.0%. Lactic acid bacteria was used in a mixed starter culture of Streptococcus salivarius ssp. thermophilus(ST36) and Lactobacillus delbrueckii ssp. bulgaricus(LB12). When the boxthorn was added with extract types, the changes of pH, acidity and lactic acid bacteria counts of yogurt during the fermenation of 3 hours were pH 5.64, titratable acidity 0.85%, 5.80xl0$\^$6/cfu/ml of viable cell counts for control yogurt, whereas those were pH 4.10∼5.06, titratable acidity 0.98∼1.27%, 1.80∼9.60x10$\^$7/ cfu/ml of viable cell counts for Lycii fructus extract yogurt. The lactose hydrolysis ratio was better for 1.0% Lycii fructus extract yogurt(42.00%) and 1.0% Lycii folium extract yogurt(41.46%) than for control yogurt(28.40%). Also, content of lactic acid of 1.0% Lycii fructus(11.9 times) and 1.0% Lycii folium extract yogurt(10.6 times) produced more than control yogurt(7.3 times). The viscosity of yogurt was better for boxthorn extract yogurt(1,027∼1,382 cps) than for control yogurt(975cps). The sensory scores of color, taste and overall acceptability of yogurt with 0.5, and 1.0% Lycii fructus extract additive were better than other groups. The yogurts made with increased Lycii fructus extract concentration(0.5∼6.0%), showed the increase of lactic acid, titratable acidity, number of lactic acid bacteria, viscosity and lactose hydrolysis rate compared to the treatments of 0.5, 1.0, 2.0, and 4.0% Lycii folium and Lycii cortex extract and powder yogurt. We gained excellent results from the yogurt to which Lycii fructus extract was added with 0.51.0% concentration.

• Food Science of Animal Resources
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• v.32 no.2
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• pp.212-219
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• 2012

The objective of this study was to investigate the effect of high pressure (HP) processing on shelf life, as well as the addition of phosvitin on lipid and protein oxidation stability of minced chicken leg meat. Minced chicken leg meat was mixed with yolk phosvitin at 500 or 1000 mg/kg meat levels, and divided into raw and cooked groups. Then, the samples were subjected to HP at 0.1, 300, and 600 MPa. The total aerobic bacteria, lipid and protein oxidation, along with instrumental meat color ($L^*$, $a^*$, and $b^*$value) of the samples were measured during storage for 7 d at $4^{\circ}C$. In raw meat, the number of total aerobic bacteria was decreased by HP at 300 MPa (4 Log reductions) and 600 MPa (5 Log reductions) after 7 d of storage (p<0.05). HP at 600 MPa increased lipid oxidation of samples at all storage days and protein oxidation of samples during storage at 3 and 7 d. HP induced the changes of meat color by increase of $L^*$ value and decrease of $a^*$ value (p<0.05). The total aerobic bacteria was not detected in the cooked samples, regardless of HP pressure, and the lipid or protein oxidation of the cooked sample treated by 600 MPa was higher than that of the control (0.1 MPa) on day 7 or control on day 3, respectively (p<0.05). The results suggested that HP can improve the shelf life of minced chicken leg meat. However, phosvitin might be a limited antioxidative agent for the improvement of oxidation stability induced by HP.