Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effect of Addition of Phosvitin and High Pressure Processing on Microbiological Quality and Lipid and Protein Oxidation of Minced Chicken Leg Meat

닭 다리 분쇄육에 초고압 처리시 Phosvitin의 첨가가 미생물학적 품질과 지방 및 단백질 산화에 미치는 효과

  • Jung, Samooel (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Kang, Min-Gu (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Kim, Il-Suk (Department of Animal Resources, Gyungnam National University of Science and Technology) ;
  • Nam, Ki-Chang (Department of Animal Science and Technology, Sunchon National University) ;
  • Ahn, Dong-Uk (Department of Agricultural Biotehcnology, Seoul National University) ;
  • Jo, Cheo-Run (Department of Animal Science and Biotechnology, Chungnam National University)
  • 정사무엘 (충남대학교 동물자원생명과학과) ;
  • 강민구 (충남대학교 동물자원생명과학과) ;
  • 김일석 (경남과학기술대학교 동물소재공학과) ;
  • 남기창 (순천대학교 동물자원과학과) ;
  • 안동욱 (서울대학교 바이오모듈레이션 전공) ;
  • 조철훈 (충남대학교 동물자원생명과학과)
  • Received : 2011.10.19
  • Accepted : 2012.03.21
  • Published : 2012.04.30
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Abstract

The objective of this study was to investigate the effect of high pressure (HP) processing on shelf life, as well as the addition of phosvitin on lipid and protein oxidation stability of minced chicken leg meat. Minced chicken leg meat was mixed with yolk phosvitin at 500 or 1000 mg/kg meat levels, and divided into raw and cooked groups. Then, the samples were subjected to HP at 0.1, 300, and 600 MPa. The total aerobic bacteria, lipid and protein oxidation, along with instrumental meat color ($L^*$, $a^*$, and $b^*$value) of the samples were measured during storage for 7 d at $4^{\circ}C$. In raw meat, the number of total aerobic bacteria was decreased by HP at 300 MPa (4 Log reductions) and 600 MPa (5 Log reductions) after 7 d of storage (p<0.05). HP at 600 MPa increased lipid oxidation of samples at all storage days and protein oxidation of samples during storage at 3 and 7 d. HP induced the changes of meat color by increase of $L^*$ value and decrease of $a^*$ value (p<0.05). The total aerobic bacteria was not detected in the cooked samples, regardless of HP pressure, and the lipid or protein oxidation of the cooked sample treated by 600 MPa was higher than that of the control (0.1 MPa) on day 7 or control on day 3, respectively (p<0.05). The results suggested that HP can improve the shelf life of minced chicken leg meat. However, phosvitin might be a limited antioxidative agent for the improvement of oxidation stability induced by HP.

본 연구는 초고압 처리가 부분육으로 유통되는 닭 다리 분쇄육의 저장 안전성에 미치는 효과와 난황 phosvitin 첨가가 지방 및 단백질의 산화 안정성에 미치는 효과를 확인하기 위하여 수행되었다. 분쇄된 닭 다리육에 phosvitin을 각각 500 및 1000 mg/kg 첨가 후 비가열 또는 가열하고, 초고압 처리(300 및 600 MPa, 5분, $15{\pm}3^{\circ}C$)에 따른 총 호기성 미생물, 지방 및 단백질산화 및 육색의 변화를 $4^{\circ}C$에서 7일간 저장 하면서 측정하였다. 연구 결과 생육에서 300 및 600 MPa 처리에 의해 총 호기성 미생물의 증식이 저장 7일 후 각각 4 및 5 Log CFU/g 정도 억제 되었으며(p<0.05), 600 MPa 처리에 의해 지방 및 단백질산화가 촉진됨이 확인되었다(p<0.05). 초고압 처리압력 증가에 따른 $L^*$ 값의 증가와 $a^*$ 및 $b^*$ 값의 감소가 확인되었다(p<0.05). 가열육은 초고압 처리와 무관하게 총 호기성 미생물의 증식이 일어나지 않았으며, 저장 7일 후 600 MPa 처리에 의한 지방의 산화가 확인되었고, 단백질의 산화는 저장 3일차에서만 600 MPa처리에 의해 유의적으로 증가함이 나타났다(p<0.05). 이상의 결과를 종합해 볼 때 초고압 처리는 부분육으로 유통되는 닭 다리 분쇄육의 저장 안전성을 높일 수 있으며, 초고압 처리시 발생하는 지방 및 단백질의 산화와 육색의 변화를 억제하기 위한 항산화제로서 phosvitin의 이용은 제한적일 것으로 생각된다.

Keywords

References

  1. Ahn, C. N., Chae, H. S., Yoo, Y. M., Cho, J. H., Kim, Y. T., Lee. J. M., and Chio, Y. I. (2003) The effect of different electrical stunning methods on meat quality in broilers. Korean J. Food Sci. An. 23, 221-226.
  2. Bover-Cid, S., Belletti, N., Garriga, M., and Aymerich, T. (2011) Model for Listeria monocytogenes inactivation on dry-cured ham by high hydrostatic pressure processing. Food Microbiol. 28, 804-809. https://doi.org/10.1016/j.fm.2010.05.005
  3. Carlez, A., Veciananogues, T., and Cheftel, J. C. (1995) Changes in Color and Myoglobin of Minced Beef Meat Due to High-Pressure Processing. LWT Food Sci. Technol. 28, 528-538. https://doi.org/10.1006/fstl.1995.0088
  4. Cava, R., Ladero, L., Gonzalez, S., Carrasco, A., and Ramirez, M. R. (2009) Effect of pressure and holding time on colour, protein and lipid oxidation of sliced dry-cured Iberian ham and loin during refrigerated storage. Innov. Food Sci. Emerg. Tehcnol. 10, 76-81. https://doi.org/10.1016/j.ifset.2008.09.005
  5. Cheah, P. B. and Ledward, D. A. (1997) Catalytic mechanism of lipid oxidation following high pressure treatment in pork fat and meat. J. Food Sci. 62, 1135-1139. https://doi.org/10.1111/j.1365-2621.1997.tb12229.x
  6. Cheftel, J. C. and Culioli, J. (1997) Effects of high pressure on meat: A review. Meat Sci. 46, 211-236. https://doi.org/10.1016/S0309-1740(97)00017-X
  7. Choi, I., Jung, C., Seog, H., and Choi, H. (2004) Purification of phosvitin from egg yolk and determination of its physicochemical properties. Food Sci. Biotechnol. 13, 434-437.
  8. Clark, R. C. (1985) The primary structure of avian phosvitins. Contributions through the Edman degradation of methylmercaptovitins prepared from the constituent phosphoproteins. Int. J. Biochem. 17, 983-988. https://doi.org/10.1016/0020-711X(85)90243-5
  9. Fagan, J. M., Sleczka, B. G., and Sohar, I. (1999) Quantitation of oxidative damage to tissue proteins. Int. J. Biochem. Cell B. 31, 751-757. https://doi.org/10.1016/S1357-2725(99)00034-5
  10. Fuentes, V., Ventanas, J., Morcuende, D., Estévez, M., and Ventanas, S. (2010) Lipid and protein oxidation and sensory properties of vacuum-packaged dry-cured ham subjected to high hydrostatic pressure. Meat Sci. 85, 506-514. https://doi.org/10.1016/j.meatsci.2010.02.024
  11. ICMSR (1986) International commission on microbiological specifications for foods. Sampling plans for fish and shellfish. In: ICMSF, Microorganisma in foods. ICMSF (ed) University of toronto Press, Toronto, Vol. 2, pp. 181-196.
  12. Ishikawa, S., Yano, Y., Arihara, K., and Itoh, M. (2004) Egg yolk phosvitin inhibits hydroxyl radical formation from the Fenton reaction. Biosci. Biotech. Biochem. 68, 1324-1331. https://doi.org/10.1271/bbb.68.1324
  13. Jung, S., Ghoul, M., and de Lamballerie-Anton, M. (2003) Influence of high pressure on the color and microbial quality of beef meat. LWT Food Sci. Technol. 36, 625-631. https://doi.org/10.1016/S0023-6438(03)00082-3
  14. Kanatt, S. R., Rao, M. S., Chawla, S. P., and Sharma, A. (2010) Shelf-life extension of convenience meat products sold in Indian supermarkets by radiation processing. Radiat. Phys. Chem. 79, 1259-1263. https://doi.org/10.1016/j.radphyschem.2010.07.008
  15. Kanner, J. (1994) Oxidative precesses in meat and meat products: quality implications. Meat Sci. 36, 169-189. https://doi.org/10.1016/0309-1740(94)90040-X
  16. Khan, M. A. S., Nakamura, S., Ogawa, M., Akita, E., Azakami, H., and Kato, A. (2000) Bactericidal action of egg yolk phosvitin against Escherichia coli under thermal stress. J. Agric. Food Chem. 48, 1503-1506. https://doi.org/10.1021/jf990700r
  17. Kim, B., Yun, H., Jung, S., Jung, Y., Jung, H., Choe, W., and Jo, C. (2011) Effect of atmospheric pressure plasma on inactivation of pathogens inoculated onto bacon using two different gas compositions. Food Microbiol. 28, 9-13. https://doi.org/10.1016/j.fm.2010.07.022
  18. Ko, K. Y., Nam, K. C., Jo, C., Lee, E. J., and Ahn, D. U. (2011) A simple and efficient method for preparing partially purified phosvitin from egg yolk using ethanol and salts. Poultry Sci. 90, 1096-1104. https://doi.org/10.3382/ps.2010-01138
  19. Korea Meat Trade Association (2011). Statistics of current meat import status. Available from: http://www.kmta.or.kr/html/sub6-1.html?scode=233&kej. Accessed Oct. 9, 2011.
  20. Kruk, Z. A., Yun, H., Rutley, D. L., Lee, E. J., Kim, Y. J., and Jo, C. (2011) The effect of high pressure on microbial population, meat quality and sensory characteristics of chicken breast fillet. Food Control 22, 6-12. https://doi.org/10.1016/j.foodcont.2010.06.003
  21. Lee, S. K., Han, J. H., and Decker, E. A. (2002) Antioxidant activity of phosvitin in phosphatidylcholine liposomes and meat model systems. J. Food Sci. 67, 37-41. https://doi.org/10.1111/j.1365-2621.2002.tb11355.x
  22. Lu, C. L. and Baker, R. C. (1986) Characteristics of egg yolk phosvitin as an antioxidant for inhibiting metal-catalyzed phospholipid oxidations. Poultry Sci. 65, 2065-2070. https://doi.org/10.3382/ps.0652065
  23. Lund M. N., Heinonen, M., Baron, C. P., and Estevez, M. (2011) Protein oxidation in muscle foods: a review. Mol. Nutr. Food Res. 55, 83-95. https://doi.org/10.1002/mnfr.201000453
  24. Ma, H. J., Ledward, D. A., Zamri, A. I., Frazier, R. A., and Zhou, G. H. (2007) Effects of high pressure/thermal treatment on lipid oxidation in beef and chicken muscle. Food Chem. 104, 1575-1579. https://doi.org/10.1016/j.foodchem.2007.03.006
  25. Omana, D. A., Plastow, G., and Betti, M. (2011) Effect of different ingredients on color and oxidative characteristics of high pressure processed chicken breast meat with special emphasis on use of beta-glucan as a partial salt replacer. Innov. Food Sci. Emerg. Technol. 12, 244-254. https://doi.org/10.1016/j.ifset.2011.04.007
  26. Orlien, V., Hansen, E., and Skibsted, L. H. (2000) Lipid oxidation in high-pressure processed chicken breast muscle during chill storage: critical working pressure in relation to oxidation mechanism. Eur. Food Res. Technol. 211, 99-104. https://doi.org/10.1007/s002179900118
  27. Park, J., Na, S., and Lee, Y. (2010) Present and future of nonthermal food processing technology. Food Sci. Ind. 75, 1-20.
  28. Pietrzak, D., Cegielka, A., Fonberg-Broczek, M., and Ziarno, M. (2011) Effects of high pressure treatment on the quality of chicken patties. High Pressure Res. 31, 350-357. https://doi.org/10.1080/08957959.2011.552503
  29. Shigehisa, T., Ohmori, T., Saito, A., Taji, S., and Hayashi, R. (1991) Effects of High Hydrostatic-Pressure on Characteristics of Pork Slurries and Inactivation of Microorganisms Associated with Meat and Meat-Products. Int. J. Food Microbiol. 12, 207-216. https://doi.org/10.1016/0168-1605(91)90071-V
  30. Souza, C. M., Boler, D. D., Clark, D. L., Kutzler, L. W., Holmer, S. F., Summerfield, J. W., Cannon, J. E., Smit, N. R., McKeith, F. K., and Killefer, J. (2011) The effects of high pressure processing on pork quality, palatability, and further processed products. Meat Sci. 87, 419-427. https://doi.org/10.1016/j.meatsci.2010.11.023
  31. Stalikas, C. D. and Konidari, C. D. (2001) Analysis of malondialdehyde in biological matrices by capillary gas chromatography with electron-capture detection and mass spectrometry. Anal. Biochem. 290, 108-115. https://doi.org/10.1006/abio.2000.4951
  32. Tarborsky, G. (1963) Interaction between phosvitin and iron and its effect on a rearrangement of phosvitin structure. Biochem.-US. 2, 266-271. https://doi.org/10.1021/bi00902a010

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