• BMB Reports
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• 제28권2호
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• pp.118-123
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• 1995

Biodegradative threonine dehydratase was purified to homogeneity from Serratia marcescens ATCC 25419 by streptomycin sulfate treatment, Sephadex G-200 gel filtration chromatography followed by AMP-Sepharose 4B affinity chromatography. The molecular weight of the purified enzyme was 118,000 by fast protein liquid chromatography using superose 6-HR. The enzyme was determined to be a homotetrameric protein with subunit molecular weights of 30,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was inhibited by ${\alpha}-Keto$ acids and their derivatives such as ${\alpha}-ketobutyrate$, pyruvate, glyoxlyate, and phosphoenol pyruvate, but not by ${\alpha}-aminobutyrate$ and ${\alpha}-hydroxybutyrate$. The inhibition of the enzyme by pyruvate and glyoxylate was observed in the presence of AMP. The inhibitory effect of glyoxylate was decreased at high enzyme concentration, whereas the inhibition by pyruvate was independent of the enzyme concentration. The kinetics of inhibition of the enzyme by pyruvate and glyoxylate revealed a noncompetitive and mixed-type inhibition by the two inhibitors with respect to L-threonine and AMP, respectively.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.573-578
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• 2005

Pyrimidine nucleotide N-ribosidase (pyrimidine 5'-nucleotide phosphoribo(deoxyribo)hydrolase/pyrimidine 5'-nucleotide nucleosidase, EC 3.2.2.10) catalyzes the breakdown of pyrimidine 5'-nucleotide into pyrimidine base and ribose(deoxyribo)-5-phosphate. However, detailed characteristics of the enzyme have not yet been reported. The enzyme was purified to homogeneity 327.9-fold with an overall yield of $6.1\%$ from Pseudomonas oleovorans ATCC 8062. The enzyme catalyzed cytidine monophosphate (CMP) and uridine monophosphate (UMP), but not adenosine monophosphate (AMP) and guanosine monophosphate (GMP). The enzyme optimally metabolized CMP at pH 6.0 and UMP at around 8.5, and the optimum temperature for the overall enzyme reaction was found to be $37^{\circ}C$. The $K_m$ values of the enzyme for CMP (at pH 6.0) and UMP (at pH 8.5) were 1.6 mM and 1.1 mM, respectively. AMP, deoxyCMP, and deoxyUMP were very effective inhibitors of the reaction. Double-reciprocal plots obtained in the absence and in the presence of AMP revealed that this inhibitory effect was of the mixed competitive type with respect to the breakdown of CMP and of the noncompetitive type with respect to the breakdown of UMP. In the presence of AMP, the enzyme followed sigmoid kinetics with respect to each substrate.

• 한국경영과학회지
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• 제40권4호
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• pp.145-156
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• 2015

Incorporation of individual learning and forgetting behaviors within worker-task assignment models produces a mixed integer nonlinear program (MINLP) problem, which is difficult to solve as a NP hard due to its nonlinearity in the objective function. Previous studies commonly assume homogeneity among workers in workforce scheduling that takes account of learning and forgetting characteristics. This paper expands previous researches by considering heterogeneous individual learning/forgetting, and investigates the impact of worker heterogeneity in initial expertise, steady-state productivity, learning and forgetting on system performance to assist manager's decision-making in worker-task assignments without tackling complex MINLP models. In order to understand the performance implications of workforce heterogeneity, this paper examines analytically how heterogeneity in each of the four parameters of the exponential learning and forgetting (L/F) model affects system performance in three cases : consecutive assignments with no break, n breaks of s-length each, and total b break-periods occurred over T periods. The study presents the direction of change in worker performance under different assignment schedules as the variance in initial expertise, steady-state productivity, learning or forgetting increases. Thus, it implies whether having more heterogenous workforce in terms of each of four parameters in the L/F model is desired or not in different schedules from the perspective of system productivity measurement.

• 한국지리정보학회지
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• 제17권2호
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• pp.121-135
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• 2014

최근 픽셀기반분류보다 더 많은 정보를 이용할 수 있는 객체기반에 대한 연구가 활발히 진행 중이다. 따라서, 본 연구는 인제군 Demilitarized Zone(DMZ)지역 일원을 대상으로 객체기반 분류기법을 이용한 토지피복분류를 실시하였다. 분류항목은 환경부 기준의 대분류 항목 7개, 중분류 항목 13개로 선정하였고, 사용된 인자는 분광 값의 평균과 표준편차, Grey Level Co-occurrence Matrix(GLCM)의 Homogeneity를 사용하여 감독분류방법 중 최근린기법을 이용하여 계층적 토지피복도를 구축하였다. 구축된 토지피복도를 이용하여 남방한계선으로부터의 거리와 Digital elevation model(DEM)을 통해 지형특성에 따른 분류항목 별 분포 특성을 분석하였다. 객체기반 분류를 위한 최적 가중치는 Scale 72, Shape 0.2. Color 0.8, Compactness 0.5, Smoothness 0.5로 선정하였고, 가중치 선정과정에서 Scale, Shape, Color가 가장 많은 영향을 주었다. 대분류 토지피복분류는 산림, 초지, 시가지의 순으로 각각 약 92%, 약 5%, 약 2%였으며, 중분류 토지피복분류는 활엽수림, 혼효림, 침엽수림의 순으로 각각 약 44%, 약 42%, 약 6% 순으로 분포하였다. 토지피복형태에 따른 분포특성을 보면, 남방한계선으로부터 2km이내의 지역에서 공공시설 지역과 도로의 이용이 높았으며, 남방한계선 6km이상의 지역에서는 논과 밭, 나지의 형태가 많았다. 산림은 표고 600m, 경사 $30^{\circ}$이상의 지역에서는 면적분포가 높았고, 농업지역과 나지, 초지는 표고 600m, 경사 $30^{\circ}$이하의 지역에서 면적분포가 높았다.

• 공업화학
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• 제24권2호
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• pp.165-170
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• 2013

초고온가스로 핵연료 구형 UCO (uranium oxycarbide) 입자 제조과정 중 중간화합물 제조에서 적용하고 있는 내부겔화공정을 대체하기 위해 외부겔화공정을 도입하는 연구가 진행 중에 있다. 외부겔화공정을 이용해서 구형 UCO입자를 제조하기 위한 사전실험으로, 중간화합물인 ADU (ammonium di-uranate) 겔 입자를 제조하기 위한 원료용액인 모사 broth 용액을 제조하여 카본블랙 입자를 분산시키는 실험이 수행되었다. 다양한 종류의 카본블랙 입자를 사용해서 모사 broth 용액에 분산실험을 수행한 결과, Cabot G 제품이 용액상에서 분산안정성과 균일한 분산상태를 나타내고 있어서, 본 연구의 카본블랙으로 선정되었다. 또한 나노크기 입자로 응집된 카본블랙 입자를 고점성 액상물질에 효율적으로 분산시키기 위해서는, 금속염용액에 카본블랙 입자를 투입하고 ultrasonic force를 이용해서 응집입자를 해체한 다음, 고점성 물질인 PVA (poly vinyl alcohol)를 투입하여 강력한 기계식혼합기를 이용해서 6000 rpm으로 2차 분산 혼합시키는 경우, ultrasonic force에 의한 broth 용액의 물성이 유지되면서 카본블랙 입자의 분산안정성과 분산상태가 양호한 broth용액을 얻을 수 있었다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2013년도 춘계학술대회 논문집
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• pp.842-848
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• 2013

Developments of Solid-State Gyroscopy during last decades are impressive and were based on thin-walled shell resonators like HRG or CRG made from fused quartz or leuko-sapphire. However, a number of design choices for inertial-grade gyroscopes, which can be used for high-g applications and for mass- or middle-scale production, is still very limited. So, considerations of fundamental physical effects in solids that can be used for development of a miniature, completely solid-state, and lower-cost sensor look urgent. There is a variety of different types of bulk acoustic (elastic) waves (BAW) in anisotropic solids. Shear waves with different variants of their polarization have to be studied especially carefully, because shear sounds in glasses and crystals are sensitive to a turn of the solid as a whole, and, so, they can be used for development of gyroscopic sensors. For an isotropic medium (for a glass or a fine polycrystalline body), classic Lame's theorem (so-called, a general solution of Elasticity Theory or Green-Lame's representation) has been modified for enough general case: an elastic medium rotated about an arbitrary set of axes. Travelling, standing, and mixed shear waves propagating in an infinite isotopic medium (or between a pair of parallel reflecting surfaces) have been considered too. An analogy with classic Foucault's pendulum has been underlined for the effect of a turn of a polarizational plane (i.e., an integration effect for an input angular rate) due to a medium's turn about the axis of the wave propagation. These cases demonstrate a whole-angle regime of gyroscopic operation. Single-crystals are anisotropic media, and, therefore, to reflect influence of the crystal's rotation, classic Christoffel-Green's tensors have been modified. Cases of acoustic axes corresponding to equal velocities for a pair of the pure-transverse (shear) waves have of an evident applied interest. For such a special direction in a crystal, different polarizations of waves are possible, and the gyroscopic effect of "polarizational precession" can be observed like for a glass. Naturally, formation of a wave pattern in a massive elastic body is much more complex due to reflections from its boundaries. Some of these complexities can be eliminated. However, a non-homogeneity has a fundamental nature for any amorphous medium due to its thermodynamically-unstable micro-structure, having fluctuations of the rapidly-frozen liquid. For single-crystalline structures, blockness (walls of dislocations) plays a similar role. Physical nature and kinematic particularities of several typical "drifts" in polarizational BAW gyros (P-BAW) have been considered briefly too. They include irregular precessions ("polarizational beats") due to: non-homogeneity of mass density and elastic moduli, dissymmetry of intrinsic losses, and an angular mismatch between propagation and acoustic axes.

• Bulletin of the Korean Chemical Society
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• 제34권6호
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• pp.1656-1662
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• 2013

The ternary phase $EuZn_xIn_{4-x}$ has been identified as the main product of reactions of Eu, Zn, and In by using the In-flux method and characterized by both powder and single-crystal X-ray diffraction. The structure belongs to the common $BaAl_4$-type (tetragonal space group I4/mmm, Pearson code tI10) with lattice parameters of a = 4.5610(9) ${\AA}$, c = 12.049(3) ${\AA}$ for composition $EuZn_{1.10(12)}In_{2.90}$ and a = 4.5463(3) ${\AA}$, c = 12.028(2) ${\AA}$ for composition $EuZn_{1.18(2)}In_{2.82}$, respectively. In this structure, the Eu atoms are situated at the center of 18-vertex Fedorov polyhedra made of Zn and In atoms, where the 4d site is preferentially occupied by In and the 4e site is occupied by randomly mixed Zn and In atoms. Theoretical investigations using tight-binding linear muffintin orbital (TB-LMTO) method provide rationale for the observed site preferences and suggest potentially wider homogeneity range than the experimentally established for $EuZn_xIn_{4-x}$ ($x{\approx}1.1$).

• 한국분말재료학회지
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• 제16권5호
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• pp.326-335
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• 2009

Fe based (Fe$_{68.2}$C$_{5.9}$Si$_{3.5}$B$_{6.7}$P$_{9.6}$Cr$_{2.1}$Mo$_{2.0}$Al$_{2.0}$) amorphous powder, which is a composition of iron blast cast slag, were produced by a gas atomization process, and sequently mixed with ductile Cu powder by a mechanical ball milling process. The Fe-based amorphous powders and the Fe-Cu composite powders were compacted by a spark plasma sintering (SPS) process. Densification of the Fe amorphous-Cu composited powders by spark plasma sintering of was occurred through a plastic deformation of the each amorphous powder and Cu phase. The SPS samples milled by AGO-2 under 500 rpm had the best homogeneity of Cu phase and showed the smallest Cu pool size. Micro-Vickers hardness of the as-SPSed specimens was changed with the milling processes.

• 한국습지학회지
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• 제12권3호
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• pp.79-97
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• 2010

본 연구의 목적은 연 증발접시 증발량의 수문학적 분해를 위하여 신경망모형을 적용하는데 있다. 신경망 모형은 각각 다층 퍼셉트론 신경망모형(MLP-NNM)과 지지벡터기구 신경망모형(SVM-NNM)으로 구성되어 있다. 그리고 신경망모형의 수행평가를 위하여 훈련 및 테스트과정으로 구성되었다. 신경망모형의 훈련과정을 위하여 실측, 모의 및 혼합자료와 같은 세 가지 형태의 자료가 사용되었으며, 테스트과정을 위해서는 실측자료만 이용되었다. 평가를 위하여 4가지의 통계학적 지표(CC, RMSE, E, AARE)가 각각 제시되었으며, ANOVA 및 Mann-Whitney U 검증을 이용하여 실측 및 계산된 월 증발접시 증발량자료에 동질성검증을 실시하였다. 본 연구를 통하여 비선형 시계열자료의 수문학적 분해를 위해서 MLP-NNM과 SVM-NNM의 적용성을 평가하였다. 게다가 연 증발접시 증발량 자료의 수문학적 분해로부터 신뢰성있는 월 증발접시 증발량자료를 구축할 수 있을 것이며, 관개배수 네트워크 시스템의 평가를 위한 이용가능한 자료를 제공할 수 있을 것이다.

• 대한화학회지
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• 제33권1호
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• pp.65-69
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• 1989

방사성 바륨 또는 방사성 란탄 추적자를 함유하고 있는 Ba(NO$_3)_2$ 와 TiO(NO$-3)_2$의 무기혼합용액을 옥살산의 에탄올 용액으로 적정하므로써 바륨티탄닐의 옥살산 염을 합성하였고, 이를 1000$^{\circ}$C에서 하소시켜 BaTiO$_3$를 만들었다. 옥살산염의 분석결과는 BaTiO(C$_2O_4)_2{\cdot}4H_2O$이며, 무기혼합용액중 Ba/Ti의 몰비가 0.950∼1.05 범위 내에서 화학양론적 결합으로 합성됨을 방사성 바륨 추적자의 도움으로 쉽게 확인하였고, Perovskite형의 구조임을 XRD로 확인하였다. 그리고 란탄 첨가제가 침전에 화학적으로 균일하게 혼입됨은 방사성 란탄의 추적자 실험으로 발견하였다. 이와 같은 실험적 사실로부터 침전물의 구성 성분의 결합이 분자준위에서 일어나고 또 티탄산바륨이 단일상의 결정임을 설명하였다.